The results make it clear that there are total 11 families, 29 genera and 43 species of spiders in the rice field of Dong Fang Hong Farm. Among them, there are 8 families, 19 genera and 28 species in the early rice fi...The results make it clear that there are total 11 families, 29 genera and 43 species of spiders in the rice field of Dong Fang Hong Farm. Among them, there are 8 families, 19 genera and 28 species in the early rice field, and 10 families, 27 genera and 36 species in the late rice field. The spatial distribution pattern of mixed spider populations in rice fields was different during different development stages of rice plant. During the prophase, metaphase and anaphase of early rice plant development, the spatial distribution pattern of mixed spider populations was aggregative, random and aggregative respectively. During the prophase, metaphase and anaphase of late rice plant development, the spatial distribution pattern was uniform, aggregative and uniform respectively.展开更多
Genetic diversity of Yunjie(.Eruca sativa Mill.)in China was assessed by analyses of RAPD(randomly amplified polymorphic DNA)markers. Twenty native cultivars representing Yunjie-growing eco-types in China were selecte...Genetic diversity of Yunjie(.Eruca sativa Mill.)in China was assessed by analyses of RAPD(randomly amplified polymorphic DNA)markers. Twenty native cultivars representing Yunjie-growing eco-types in China were selected as material in this study. Twelve out of the 64 tested random decamer primers were able to identify 131 stable RAPD bands from these Yunjie cultivars. Of them 105 bands, or 80.15% of the total, were polymorphic. Most Yunjie cultivars from the same ecotype had their characteristic DNA bands. Cluster analysis by unweighted pair group method of arithmetic means(UPGMA)suggested that the 20 Yunjie genotypes could be divided into four groups. The genetic distances among the 20 cultivars varied from 0.117 8 between Shuozhou and Shenchi to 0. 499 4 between Hetian and Xiliang. Hetian alone could be a new type of Yunjie identified in China because it had the greatest genetic distance from all the other tested cultivars. These results indicate that Chinese Yunjie have abundant genetic diversity. Classification of Chinese Yunjie based on the RAPD information was in good agreement with the relationships between these Yunjie cultivars in their geographic origins and their plant morphology.展开更多
The grain-filling characteristics of six rice varieties (combinations) and the relationships between their relative biochemistry composition during phytate synthesizing and grain plumpness were studied. Regarding resu...The grain-filling characteristics of six rice varieties (combinations) and the relationships between their relative biochemistry composition during phytate synthesizing and grain plumpness were studied. Regarding results for ISHR1, ISHR2, R198 and JW21, with good grain plumpness, the two-step-filling in superior spikelets and inferior spikelets was not clear, while for ISHR3 and 559, with poor grain plumpness, it was very clear. From booting stage to flowering stage, the contents of phytate and inositol in varieties with good grain plumpness was obviously higher than those in varieties with poor grain plumpness. While at grain filling stage, the content of inorganic phosphorus in varieties with poor grain plumpness was obviously higher than that in varieties with good grain plumpness. The contents of phytate and inositol from booting stage to flowering stage was highly significantly correlated with the initial filling power (R_0), the mean filling rate (R_M) and grain filling percentage (P_(GF)), and the content of inorganic phosphorus at grain filling stage was negatively significantly correlated with R_0, F_M and P_(GF). Furthermore, effective approach to improving grain filling was put forward.展开更多
Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker,and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic ...Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker,and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic transformation system at various osmotica treatments.The results revealed that the calli induced from tomato young leaves and rice young spikelets were the ideal transfomation acceptor.The cells of calli were still vigorous when treated with 0.75 mol/L hyperosmotic solution for 4 hours.The differentiation rates of calli varied from 7.5% to 93.3% in different media.The bud differentiation was apparently inhibited by hyperosmotic treatments.0.75mol/L sucrose hypertonic solution and 0.2mol/L CaCl2 solution were favorable hyperosmoticum and hypcosmoticum respectively.展开更多
Abstract: To explore the mode of the spatio-temporal expression of six newly discovered ginsenoside biosynthesis candidate gene transcripts, both Northern blotting and semi-quantitative reverse transcription-polymeras...Abstract: To explore the mode of the spatio-temporal expression of six newly discovered ginsenoside biosynthesis candidate gene transcripts, both Northern blotting and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) were used to elucidate the mRNA expression levels of the transcripts in various tissues and organs of Panax ginseng C. A. Meyer during different growth development stages. The six gene transcripts were all differentially expressed in cultured callus, root, stem, leaf, and seed. The mRNA expression levels were significantly higher in four-year-old roots than in one-year-old roots, and results of semi-quantitative RT-PCR assays were in accordance with those of Northern blotting analyses. The results strongly suggest that all six genes were differentially expressed at root-specific developmental stages. In particular, when a quiescent early stage culture suspension of P. ginseng cells was exposed to the ginsenoside biosynthesis-promoting elicitor Aspergillus niger polysaccharide, the GBR6 gene transcript response showed time-dependent increments and was parallel with ginsenoside productivity (P < 0.01). Overexpressionof the GBR6 gene is likely to play a critically important role in the biosynthesis of ginsenosides. The results of the present study provided a background for the further elucidation of the structure and physiological function of these six candidate genes.展开更多
The phytophagous insect, Epiblema strenuana, is an effective biocontrol agent against Ambrosia and Parthenium, and was introduced to China in 1990 to control Ambrosia artemisiifolia. After a small scale release in Hun...The phytophagous insect, Epiblema strenuana, is an effective biocontrol agent against Ambrosia and Parthenium, and was introduced to China in 1990 to control Ambrosia artemisiifolia. After a small scale release in Hunan Province, E. strenuana established and begun to spread. Host specificity and feeding studies in Hunan have indicated that there are four host plants in China, A. artemisiifolia, A. trifida, Xanthium sibiricum and Parthenium hysterophorus. These plants are all weeds that need to be controlled. However, there are still some different views among scientists about the merits of this release because of potential effects on non-target species such as Helianthus annuus, and uncertainty about climatic suitability. To address these concerns, the CLIMEX system was used to predict the possibility of establishment of E. strenuana with A. artemisiifolia, A. trifida, and P. hysterophorus at 85 meteorological locations. Also, the probability of E. strenuana co-establishing with A. artemisiifolia, A. trifida or P. hysterophorus was evaluated quantitatively by bioclimatic risk index (BCRI) transferred from ecoclimate index (EI). Moreover, the hypothetical adaptation of E. strenuana to H. annuus as a host, were evaluated by bioclimate matching. These results help to predict the potential spread of A. artemisiifolia, A. trifida, P. hysterophorus and evaluate the merits of further releases of E. strenuana in China.展开更多
s: The current status of AEE in Hunan Province is introduced in brief. The reasons why the AEE was polluted and destroyed are analyzed; the countermeasures for improving AEE are brought forward.
Optimum technical parameters for extracting lycopene from tomatoes are studied with orthogonal experiments.The results show that chloroform has the best effect compared to 4 other groups of solvents:petroleum ether,ac...Optimum technical parameters for extracting lycopene from tomatoes are studied with orthogonal experiments.The results show that chloroform has the best effect compared to 4 other groups of solvents:petroleum ether,acetic add + ethanol,acetic ether,and acetone + diethyl ether,associated with the parameters of 40℃,pH 5 and the ratio of tomato paste to solvent 1:1.展开更多
Four concentrations of CCC were used to treat pepper seedlings.The results indicated that 50-150mg·L-1 CCC decreased the content of malonaldehydic acid (MDA) and increased the content of soluble protein,ascorbic ...Four concentrations of CCC were used to treat pepper seedlings.The results indicated that 50-150mg·L-1 CCC decreased the content of malonaldehydic acid (MDA) and increased the content of soluble protein,ascorbic acid (AsA) and glutathion(GSH),and activity of superoxide dismutase (SOD) and catalase (CAT),whereas 200mg·L-1 CCC caused the soluble protein content and the CAT activity of Xiangyan 1 and the AsA content of Xiangyan 10 to descend.The comprehensive evaluation manifested that 100-150mg·L-1 CCC had obvious effects on water-logging resistance of pepper.Different varieties required different concentrations,eg,l00mg·L-1 CCC suited Xiangyan 1,while 150 mg·L-1 CCC suited Xiangyan 10.展开更多
Through a batch experiment, the mobility and speciation of heavy metals(Cd, Cu, Zn) in two acidic forest soils from Hunan Province were studied. The results showed that the release and potential active speciation of C...Through a batch experiment, the mobility and speciation of heavy metals(Cd, Cu, Zn) in two acidic forest soils from Hunan Province were studied. The results showed that the release and potential active speciation of Cd, Cu, and Zn in the tested contaminated red soil(CRS) and yellow red soil(CYRS) increased significantly with pH decreasing and ion concentrations increasing of simulated acid rain, and these effects were mainly decided by the pH value of simulated acid rain. Cd had the highest potential risk on the environment compared with Cu and Zn. Cd existed mainly in exchangeable form in residual CRS and CYRS, Cu in organically bound and Mn-oxide occluded forms, and Zn in mineral forms due to the high background values.展开更多
Different ploidy ramie materials were studied via microscopic observation on stem sections and macerated fiber cells. Morphologic differences between different ploidy plants were analyzed. Average bark area percentage...Different ploidy ramie materials were studied via microscopic observation on stem sections and macerated fiber cells. Morphologic differences between different ploidy plants were analyzed. Average bark area percentages in ramie stem transection of haploids, tetraploids, diploids and triploids were 28. 91, 27. 05, 26. 97 and 24.77% respectively. Average percentages of fiber layer area of diploids, haploids, tetraploids and triploids were 16.80, 16. 58, 15.52 and 13.78% respectively. Average fiber cell diameter and cell wall thickness were increased along with the increase of the ploidy of the plants. Average fiber length of diploids, triploids and haploids were 8. 49, 7. 96 and 6. 93 cm respectively. Average L/B (length/breadth) of diploids, triploids and haploids were 2 470. 7, 2 390. 6 and 1 616.3 respectively. Average breadths of fiber of haploids, diploids and triploids were 29. 30, 33. 87 and 49. 20μm respectively. However, there were relatively large variations in the above characteristics among the ramie materials of the same ploidy levels. Field performance of different ploidy plants was also investigated. As the chromosome ploidy increasing, there was a tendency of declining in shoot number per plant and increasing in stem diameter. Average shoots per plant of haploids, diploids, triploids and tetraploids were 5.83, 5.30, 3.77 and 3. 65 whereas their average stem diameters were 0.66, 0. 67, 0. 74 and 0. 76 cm respectively. Triploids were the tallest, while haploids were the shortest. Triploids had strong growth vigour, diploids and tetraploids had moderate growth vigour, while haploids appeared to be lack of growth vigour. Cold stress tolerance of tetraploids were the strongest, diploid had the moderate tolerance, while haploids and triploids were the least tolerant to cold stress. Both haploids and triploids were sterile.展开更多
Two G. somalense monosomic alien addition lines were identified from the derived backcross progenies of allohexaploid between G. hirsutum and G. somalense through cytological and morphological observation. Furthermore...Two G. somalense monosomic alien addition lines were identified from the derived backcross progenies of allohexaploid between G. hirsutum and G. somalense through cytological and morphological observation. Furthermore, the alien addition chromosome was identified and distinguished by RAPD analysis. A total of 160 RAPD primers were used for PCR amplification. Primer SBSG11 could produce a spe-cific molecular marker (600 bp) for monosomic alien addi-tion line Ⅰ(MAAL Ⅰ). Primer SBSC03 could produce a specific molecular marker (700 bp) for monosomic alien ad-dition line Ⅱ(MAAL Ⅱ). SBSE07 and SBSE08 could re-spectively produce common molecular marker for mono-somic alien addition lines Ⅰ and Ⅱ. G . somalense alien addition lines could be important for cotton improvement.展开更多
Two hybrid hot pepper varieties Xiangyan 5 and Xiangyan 10, and their parents were analyzed the polymerase chain reaction with MJ /PT 200 Peltrier Themal Cycler and DS 800 White-ultravilot Transilluminator to set up a...Two hybrid hot pepper varieties Xiangyan 5 and Xiangyan 10, and their parents were analyzed the polymerase chain reaction with MJ /PT 200 Peltrier Themal Cycler and DS 800 White-ultravilot Transilluminator to set up a RAPD system adaptable to the purity determination of the hy-brid seeds. Among the 39 random primers, 2 and 4 primers were found to be used effectively in Xiangyan 5 and Xiangyan 10 respectively.展开更多
The expression of Arabidopsis PDF1.2 gene is regulated by jasmonic acid (JA) and ethylene (ET). It also has been well documented that GCC box is an element re- sponsive to ET,however, the responsive mechanism of JA in...The expression of Arabidopsis PDF1.2 gene is regulated by jasmonic acid (JA) and ethylene (ET). It also has been well documented that GCC box is an element re- sponsive to ET,however, the responsive mechanism of JA in such plant defense gene expression is unclear. In this paper, the authors define the essential cis-acting element in PDF1.2 promoter responsive to methyl jasmonate (MeJA) through fragment deletions and site-directed mutageneses combining Agrobacterium-mediated transient reporter gene expression in tobacco leaves. Firstly, the MeJA inducible expression of PDF1.2 was confirmed by using the upstream ?1.86 kb fragment of PDF1.2 gene. Secondly, the upstream –300— ?243 bp fragment of the promoter was evidenced to respond to MeJA. To further characterize this promoter region, three point mutations were introduced into the –300—?243 bp fragment of the promoter. This result showed that the muta- tion of GCC box abolished MeJA induction, whereas the mutations of the G box-like and the imperfect palindrome sequence did not significantly decrease MeJA inducible effect, indicating that GCC box in PDF1.2 is essential for MeJA induction. The sufficient responsiveness to MeJA of this GCC box was further investigated by 4×GCC fused up- stream to the CaMV 35S minimal promoter. This result sug- gested that the fused promoter was able to activate reporter gene expression in response to MeJA. Thus these results in- dicate that the GCC box in PDF1.2 is an essential and suffi- cient element to confer MeJA induction.展开更多
文摘The results make it clear that there are total 11 families, 29 genera and 43 species of spiders in the rice field of Dong Fang Hong Farm. Among them, there are 8 families, 19 genera and 28 species in the early rice field, and 10 families, 27 genera and 36 species in the late rice field. The spatial distribution pattern of mixed spider populations in rice fields was different during different development stages of rice plant. During the prophase, metaphase and anaphase of early rice plant development, the spatial distribution pattern of mixed spider populations was aggregative, random and aggregative respectively. During the prophase, metaphase and anaphase of late rice plant development, the spatial distribution pattern was uniform, aggregative and uniform respectively.
基金funded by the Natural Science Fund of Gansu Province,China(ZS991-A21-043-N)
文摘Genetic diversity of Yunjie(.Eruca sativa Mill.)in China was assessed by analyses of RAPD(randomly amplified polymorphic DNA)markers. Twenty native cultivars representing Yunjie-growing eco-types in China were selected as material in this study. Twelve out of the 64 tested random decamer primers were able to identify 131 stable RAPD bands from these Yunjie cultivars. Of them 105 bands, or 80.15% of the total, were polymorphic. Most Yunjie cultivars from the same ecotype had their characteristic DNA bands. Cluster analysis by unweighted pair group method of arithmetic means(UPGMA)suggested that the 20 Yunjie genotypes could be divided into four groups. The genetic distances among the 20 cultivars varied from 0.117 8 between Shuozhou and Shenchi to 0. 499 4 between Hetian and Xiliang. Hetian alone could be a new type of Yunjie identified in China because it had the greatest genetic distance from all the other tested cultivars. These results indicate that Chinese Yunjie have abundant genetic diversity. Classification of Chinese Yunjie based on the RAPD information was in good agreement with the relationships between these Yunjie cultivars in their geographic origins and their plant morphology.
文摘The grain-filling characteristics of six rice varieties (combinations) and the relationships between their relative biochemistry composition during phytate synthesizing and grain plumpness were studied. Regarding results for ISHR1, ISHR2, R198 and JW21, with good grain plumpness, the two-step-filling in superior spikelets and inferior spikelets was not clear, while for ISHR3 and 559, with poor grain plumpness, it was very clear. From booting stage to flowering stage, the contents of phytate and inositol in varieties with good grain plumpness was obviously higher than those in varieties with poor grain plumpness. While at grain filling stage, the content of inorganic phosphorus in varieties with poor grain plumpness was obviously higher than that in varieties with good grain plumpness. The contents of phytate and inositol from booting stage to flowering stage was highly significantly correlated with the initial filling power (R_0), the mean filling rate (R_M) and grain filling percentage (P_(GF)), and the content of inorganic phosphorus at grain filling stage was negatively significantly correlated with R_0, F_M and P_(GF). Furthermore, effective approach to improving grain filling was put forward.
文摘Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker,and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic transformation system at various osmotica treatments.The results revealed that the calli induced from tomato young leaves and rice young spikelets were the ideal transfomation acceptor.The cells of calli were still vigorous when treated with 0.75 mol/L hyperosmotic solution for 4 hours.The differentiation rates of calli varied from 7.5% to 93.3% in different media.The bud differentiation was apparently inhibited by hyperosmotic treatments.0.75mol/L sucrose hypertonic solution and 0.2mol/L CaCl2 solution were favorable hyperosmoticum and hypcosmoticum respectively.
文摘Abstract: To explore the mode of the spatio-temporal expression of six newly discovered ginsenoside biosynthesis candidate gene transcripts, both Northern blotting and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) were used to elucidate the mRNA expression levels of the transcripts in various tissues and organs of Panax ginseng C. A. Meyer during different growth development stages. The six gene transcripts were all differentially expressed in cultured callus, root, stem, leaf, and seed. The mRNA expression levels were significantly higher in four-year-old roots than in one-year-old roots, and results of semi-quantitative RT-PCR assays were in accordance with those of Northern blotting analyses. The results strongly suggest that all six genes were differentially expressed at root-specific developmental stages. In particular, when a quiescent early stage culture suspension of P. ginseng cells was exposed to the ginsenoside biosynthesis-promoting elicitor Aspergillus niger polysaccharide, the GBR6 gene transcript response showed time-dependent increments and was parallel with ginsenoside productivity (P < 0.01). Overexpressionof the GBR6 gene is likely to play a critically important role in the biosynthesis of ginsenosides. The results of the present study provided a background for the further elucidation of the structure and physiological function of these six candidate genes.
基金supported by National Natural Science Foundation of China(39770510)National 10th“Five-year Plan”Key Research Program(2001BA611B-06-1-5).
文摘The phytophagous insect, Epiblema strenuana, is an effective biocontrol agent against Ambrosia and Parthenium, and was introduced to China in 1990 to control Ambrosia artemisiifolia. After a small scale release in Hunan Province, E. strenuana established and begun to spread. Host specificity and feeding studies in Hunan have indicated that there are four host plants in China, A. artemisiifolia, A. trifida, Xanthium sibiricum and Parthenium hysterophorus. These plants are all weeds that need to be controlled. However, there are still some different views among scientists about the merits of this release because of potential effects on non-target species such as Helianthus annuus, and uncertainty about climatic suitability. To address these concerns, the CLIMEX system was used to predict the possibility of establishment of E. strenuana with A. artemisiifolia, A. trifida, and P. hysterophorus at 85 meteorological locations. Also, the probability of E. strenuana co-establishing with A. artemisiifolia, A. trifida or P. hysterophorus was evaluated quantitatively by bioclimatic risk index (BCRI) transferred from ecoclimate index (EI). Moreover, the hypothetical adaptation of E. strenuana to H. annuus as a host, were evaluated by bioclimate matching. These results help to predict the potential spread of A. artemisiifolia, A. trifida, P. hysterophorus and evaluate the merits of further releases of E. strenuana in China.
文摘s: The current status of AEE in Hunan Province is introduced in brief. The reasons why the AEE was polluted and destroyed are analyzed; the countermeasures for improving AEE are brought forward.
文摘Optimum technical parameters for extracting lycopene from tomatoes are studied with orthogonal experiments.The results show that chloroform has the best effect compared to 4 other groups of solvents:petroleum ether,acetic add + ethanol,acetic ether,and acetone + diethyl ether,associated with the parameters of 40℃,pH 5 and the ratio of tomato paste to solvent 1:1.
文摘Four concentrations of CCC were used to treat pepper seedlings.The results indicated that 50-150mg·L-1 CCC decreased the content of malonaldehydic acid (MDA) and increased the content of soluble protein,ascorbic acid (AsA) and glutathion(GSH),and activity of superoxide dismutase (SOD) and catalase (CAT),whereas 200mg·L-1 CCC caused the soluble protein content and the CAT activity of Xiangyan 1 and the AsA content of Xiangyan 10 to descend.The comprehensive evaluation manifested that 100-150mg·L-1 CCC had obvious effects on water-logging resistance of pepper.Different varieties required different concentrations,eg,l00mg·L-1 CCC suited Xiangyan 1,while 150 mg·L-1 CCC suited Xiangyan 10.
文摘Through a batch experiment, the mobility and speciation of heavy metals(Cd, Cu, Zn) in two acidic forest soils from Hunan Province were studied. The results showed that the release and potential active speciation of Cd, Cu, and Zn in the tested contaminated red soil(CRS) and yellow red soil(CYRS) increased significantly with pH decreasing and ion concentrations increasing of simulated acid rain, and these effects were mainly decided by the pH value of simulated acid rain. Cd had the highest potential risk on the environment compared with Cu and Zn. Cd existed mainly in exchangeable form in residual CRS and CYRS, Cu in organically bound and Mn-oxide occluded forms, and Zn in mineral forms due to the high background values.
文摘Different ploidy ramie materials were studied via microscopic observation on stem sections and macerated fiber cells. Morphologic differences between different ploidy plants were analyzed. Average bark area percentages in ramie stem transection of haploids, tetraploids, diploids and triploids were 28. 91, 27. 05, 26. 97 and 24.77% respectively. Average percentages of fiber layer area of diploids, haploids, tetraploids and triploids were 16.80, 16. 58, 15.52 and 13.78% respectively. Average fiber cell diameter and cell wall thickness were increased along with the increase of the ploidy of the plants. Average fiber length of diploids, triploids and haploids were 8. 49, 7. 96 and 6. 93 cm respectively. Average L/B (length/breadth) of diploids, triploids and haploids were 2 470. 7, 2 390. 6 and 1 616.3 respectively. Average breadths of fiber of haploids, diploids and triploids were 29. 30, 33. 87 and 49. 20μm respectively. However, there were relatively large variations in the above characteristics among the ramie materials of the same ploidy levels. Field performance of different ploidy plants was also investigated. As the chromosome ploidy increasing, there was a tendency of declining in shoot number per plant and increasing in stem diameter. Average shoots per plant of haploids, diploids, triploids and tetraploids were 5.83, 5.30, 3.77 and 3. 65 whereas their average stem diameters were 0.66, 0. 67, 0. 74 and 0. 76 cm respectively. Triploids were the tallest, while haploids were the shortest. Triploids had strong growth vigour, diploids and tetraploids had moderate growth vigour, while haploids appeared to be lack of growth vigour. Cold stress tolerance of tetraploids were the strongest, diploid had the moderate tolerance, while haploids and triploids were the least tolerant to cold stress. Both haploids and triploids were sterile.
文摘Two G. somalense monosomic alien addition lines were identified from the derived backcross progenies of allohexaploid between G. hirsutum and G. somalense through cytological and morphological observation. Furthermore, the alien addition chromosome was identified and distinguished by RAPD analysis. A total of 160 RAPD primers were used for PCR amplification. Primer SBSG11 could produce a spe-cific molecular marker (600 bp) for monosomic alien addi-tion line Ⅰ(MAAL Ⅰ). Primer SBSC03 could produce a specific molecular marker (700 bp) for monosomic alien ad-dition line Ⅱ(MAAL Ⅱ). SBSE07 and SBSE08 could re-spectively produce common molecular marker for mono-somic alien addition lines Ⅰ and Ⅱ. G . somalense alien addition lines could be important for cotton improvement.
文摘Two hybrid hot pepper varieties Xiangyan 5 and Xiangyan 10, and their parents were analyzed the polymerase chain reaction with MJ /PT 200 Peltrier Themal Cycler and DS 800 White-ultravilot Transilluminator to set up a RAPD system adaptable to the purity determination of the hy-brid seeds. Among the 39 random primers, 2 and 4 primers were found to be used effectively in Xiangyan 5 and Xiangyan 10 respectively.
文摘The expression of Arabidopsis PDF1.2 gene is regulated by jasmonic acid (JA) and ethylene (ET). It also has been well documented that GCC box is an element re- sponsive to ET,however, the responsive mechanism of JA in such plant defense gene expression is unclear. In this paper, the authors define the essential cis-acting element in PDF1.2 promoter responsive to methyl jasmonate (MeJA) through fragment deletions and site-directed mutageneses combining Agrobacterium-mediated transient reporter gene expression in tobacco leaves. Firstly, the MeJA inducible expression of PDF1.2 was confirmed by using the upstream ?1.86 kb fragment of PDF1.2 gene. Secondly, the upstream –300— ?243 bp fragment of the promoter was evidenced to respond to MeJA. To further characterize this promoter region, three point mutations were introduced into the –300—?243 bp fragment of the promoter. This result showed that the muta- tion of GCC box abolished MeJA induction, whereas the mutations of the G box-like and the imperfect palindrome sequence did not significantly decrease MeJA inducible effect, indicating that GCC box in PDF1.2 is essential for MeJA induction. The sufficient responsiveness to MeJA of this GCC box was further investigated by 4×GCC fused up- stream to the CaMV 35S minimal promoter. This result sug- gested that the fused promoter was able to activate reporter gene expression in response to MeJA. Thus these results in- dicate that the GCC box in PDF1.2 is an essential and suffi- cient element to confer MeJA induction.
