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利用RNAi技术抑制籽粒PPO合成改良小麦面粉白度的研究 被引量:7
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作者 孙重霞 杨凤萍 +5 位作者 张婷 隋晓燕 梁荣奇 LIU Qing 张晓东 李保云 《中国农业科学》 CAS CSCD 北大核心 2013年第6期1104-1113,共10页
【目的】通过RNAi策略抑制小麦籽粒ppo的表达,获得籽粒PPO酶活性低、白度高的转基因小麦新种质。【方法】构建了以小麦胚乳特异表达启动子1Dx5启动子驱动的籽粒ppo的RNAi载体pBAC47P-ppoIR,利用基因枪将该载体与含bar的表达载体基因枪... 【目的】通过RNAi策略抑制小麦籽粒ppo的表达,获得籽粒PPO酶活性低、白度高的转基因小麦新种质。【方法】构建了以小麦胚乳特异表达启动子1Dx5启动子驱动的籽粒ppo的RNAi载体pBAC47P-ppoIR,利用基因枪将该载体与含bar的表达载体基因枪共转化中优9507幼胚愈伤组织,获得T0转基因植株。通过分子鉴定(PCR、Southern杂交、半定量RT-PCR、Northern杂交)、酶活筛选和白度测定等方法对转基因植株及其后代株系进行筛选。【结果】获得了27株阳性T0转基因植株。转基因植株及其后代经PCR和Southern杂交检测表明,RNAi构件已经稳定整合到T1的12个株系中。半定量RT-PCR和Northern杂交结果表明有20株T2籽粒中ppo表达水平明显低于对照。对转基因T2灌浆期籽粒进行PPO同功酶活性分析表明,有6个株系的PPO活性有所减弱。对T4籽粒的面片白度测定表明,5个株系的白度均比对照有所提高,其中2个株系效果显著。【结论】RNAi技术的表达能抑制籽粒ppo表达,降低PPO同功酶的活性,明显提高小麦面片白度,从而为小麦面粉白度的改良、品质育种提供了材料。 展开更多
关键词 小麦 多酚氧化酶(PPO) RNAI 白度
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Ocular manifestation and their associated factors among HIV/AIDS patients receiving highly active antiretroviral therapy in Southern Ethiopia 被引量:2
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作者 Anteneh Amsalu Kindie Desta +1 位作者 Demiss Nigussie Demoze Delelegne 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2017年第5期776-781,共6页
AIM:To assess the pattern of ocular manifestation and associated factors among human immunodeficiency virus(HIV)/acquired immunodeficiency syndrome(AIDS) patients on highly active antiretroviral therapy(HAART) ... AIM:To assess the pattern of ocular manifestation and associated factors among human immunodeficiency virus(HIV)/acquired immunodeficiency syndrome(AIDS) patients on highly active antiretroviral therapy(HAART) at Hawassa University Referral Hospital, Southern Ethiopia. METHODS:A cross sectional study was conducted from January 2014 to April 2015. After obtaining informed written consent, 240 adult HIV/AIDS patients on HAART were randomly selected regardless of their ophthalmic symptoms, WHO status or CD4 count. Data were collected using structured questionnaires and ophthalmologic clinical examination. Data were entered and analyzed using SPSS version 20.0 software. RESULTS:The mean duration of HAART was 62.5mo. The prevalence of HIV related ocular manifestation was 14.2%. Seborrheic blepharitis(5%) was the most common ocular manifestation, followed by squamoid conjunctival growth(3.8%). The rate of ocular manifestation was significantly higher among study participants who had CD4+ count 〈200 cells/μL(AOR=3.83; 95%CI:1.315-11.153), low duration of HAART(AOR=3.0; 95%CI:1.305-6.891) and who had primary school education [odds ratio(OR) =2.8; 95%CI:1.105-7.099]. Prevalence of visual impairment and blindness was 10.9% and 5.8%, respectively.CONCLUSION:HAART may be the reason for the decline in the prevalence of ocular manifestation in HIV/AIDS patients in the study area. Ophthalmologic screening of HIV/AIDS patients, especially those with CD4 counts of 〈200/μL cells and in the first five years of HAART followup is recommended to reduce visual impairment and/or blindness. 展开更多
关键词 human immunodeficiency virus acquired immunodeficiency syndrome ocular manifestation highlyactive antiretroviral therapy
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坦桑尼亚耐热抗旱水稻无公害高产栽培技术试验初报 被引量:3
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作者 王骞 文守云 +7 位作者 Sophia Kashenge Jackson Madulu Nkuba 陈华林 邹彪 李贤辉 甘义 钟水明 崔玉 《南方农业》 2017年第28期57-59,共3页
水源充裕和适宜稻作的非洲国家,水稻是主要农作物之一,在东非坦桑尼亚,水稻是第二大粮食作物,Txd306是当地种植面积最大的主推品种,农业推广机构试验产量通常为5~6 t·hm^(-2)。为了验证中国水稻无公害高产栽培技术在坦桑尼亚的应... 水源充裕和适宜稻作的非洲国家,水稻是主要农作物之一,在东非坦桑尼亚,水稻是第二大粮食作物,Txd306是当地种植面积最大的主推品种,农业推广机构试验产量通常为5~6 t·hm^(-2)。为了验证中国水稻无公害高产栽培技术在坦桑尼亚的应用表现,专家组于2016年2—7月进行试验示范,在栽培上采用了合理密植、配方施肥、科学用水和病虫防治等无公害水稻栽培集成技术,理论产量达到9.079 t·hm^(-2),实收产量9.825 t,试验结果表明,在非洲推广水稻无公害高产栽培技术具有较好前景。 展开更多
关键词 水稻 耐热抗旱 无公害 高产栽培 坦桑尼亚
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Forecasting the Ethiopian Coffee Price Using Kalman Filtering Algorithm 被引量:2
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作者 Tesfahun Berhane Nurilign Shibabaw +2 位作者 Aemiro Shibabaw Molalign Adam Abera A.Muhamed 《Journal of Resources and Ecology》 CSCD 2018年第3期302-305,共4页
Ethiopian coffee price is highly fluctuated and has significant effect on the economy of the country. Conducting a research on forecasting coffee price has theoretical and practical importance.This study aims at forec... Ethiopian coffee price is highly fluctuated and has significant effect on the economy of the country. Conducting a research on forecasting coffee price has theoretical and practical importance.This study aims at forecasting the coffee price in Ethiopia. We used daily closed price data of Ethiopian coffee recorded in the period 25 June 2008 to 5 January 2017 obtained from Ethiopia commodity exchange(ECX) market to analyse coffee prices fluctuation. Here, the nature of coffee price is non-stationary and we apply the Kalman filtering algorithm on a single linear state space model to estimate and forecast an optimal value of coffee price. The performance of the algorithm for estimating and forecasting the coffee price is evaluated by using root mean square error(RMSE). Based on the linear state space model and the Kalman filtering algorithm, the root mean square error(RMSE) is 0.000016375, which is small enough, and it indicates that the algorithm performs well. 展开更多
关键词 coffee price forecasting state space model Kalman filtering algorithm ETHIOPIAN
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Response of soil mite abundance and diversity to a monospecific timber Tectona grandis plantation in Ivory Coast 被引量:3
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作者 Julien Kouadio N'DRI Henri Marc ANDRE +2 位作者 Jan LAGERLOEF Jerome Ebagnerin TONDOH Thierry HANCE 《Current Zoology》 SCIE CAS CSCD 2013年第5期633-643,共11页
This study aims to assess the impact of monospecific Tectona grandis forest plantation on the soil mite abundance and diversity. To achieve these objectives, two sites situated in Ivory Coast were investigated. The fi... This study aims to assess the impact of monospecific Tectona grandis forest plantation on the soil mite abundance and diversity. To achieve these objectives, two sites situated in Ivory Coast were investigated. The first, a primary forest was characterized by a very weak human activities whereas the second, a teak plantation was characterized by a high disturbance performed during the planting. After extracting, sorted and description, 116 mite species were described in the two sites. Mite densities were lower in teak plantation and also higher in the litter and decreased to the depth in both sites. Species richness re- corded in teak plantation (52 species) was significantly lower compared to primary forest (98 species). The same trend was ob- served for Oribatida but not for Gamasida. The lower Oribatida (5 vs. 17) and higher Oribatida (24 vs. 41) were recorded respec- tively in teak plantation and primary forest. Mite Shannon index and evenness were significantly different between sites. High Jaccard index values and the appearance of exclusive species in both habitats showed that the sites are very distinct. Total number of species recorded corresponded to 58%-63% of the total number of species estimated by ACE and Chao 1&2 estimators, indi- cating that the sampling effort was not sufficient. Mite abundance and diversity varied depending on the characteristics of habi- tats. Chemical element (Corn, Ctot, Ntot, and SOM) values were lower in teak plantation (disturbed habitat) and significantly dif- ferent to primary forest in the topsoil. Apart from litter height, soil depth, pH and C/N ratio, others variables were strongly corre- lated to mite abundance and diversity [Current Zoology 59 (5): 633-643, 2013]. 展开更多
关键词 Soil mite Teak plantation Diversity and community structure Disturbance.
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Dynamics of risers for earthquake resistant designs 被引量:1
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作者 Duan Menglan Wang Yi +2 位作者 Yue Zhiyong Estefen Segen Yang Xiaogang 《Petroleum Science》 SCIE CAS CSCD 2010年第2期273-282,共10页
It is well known that no criterion about seismic design for risers is available, and relevant research has not been reported. A comprehensive study of riser dynamics during earthquakes is performed in this paper. A dy... It is well known that no criterion about seismic design for risers is available, and relevant research has not been reported. A comprehensive study of riser dynamics during earthquakes is performed in this paper. A dynamic model for seismic analysis of risers is developed in accordance with the working environment of the risers and the influence of inertia force of the pipelines. The dynamic equations for the developed model are derived and resolved on the basis of the energy theory of beams. Numerical simulation for an engineering project in the Bohai Oil Field, China shows that the fundamental frequency of the riser plays the major role in the seismic responses, and for platforms in shallow water in Bohai Bay, the risers demonstrate a much lower stress response due to prominent differences between the riser frequency and the earthquake wave frequency. The presented model and its corresponding method for seismic analysis are practical and important for riser design resistant to earthquake waves. 展开更多
关键词 Subsea pipeline RISER seismic design dynamic response earthquake wave Hamilton theory
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使用荧光、UV和DOC测量法监测废水中DOM的生物降解能力
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作者 Ibrahim Saadi 葛秀珍(翻译) 苑惠明(校对) 《水文地质工程地质技术方法动态》 2007年第5期1-9,共9页
用一系列试验评价废水中DOM(溶解性有机物)的微生物降解的潜力。废水样从Haifa废水处理站和Qishon水库采集,以2-4个月为一个周期,或者用废水或者用土壤微生物对水样进行培养,其特征用溶解性有机碳含量(DOC)、UV254吸光率和激发荧... 用一系列试验评价废水中DOM(溶解性有机物)的微生物降解的潜力。废水样从Haifa废水处理站和Qishon水库采集,以2-4个月为一个周期,或者用废水或者用土壤微生物对水样进行培养,其特征用溶解性有机碳含量(DOC)、UV254吸光率和激发荧光-辐射基质表示。根据腐殖质/棕黄酸成分和似蛋白质结构,确定了三个主要的荧光峰值。在生物降解过程中,不同程度地增加了三个特殊荧光峰值,本文建议选择非发光成分。在一些实例中,发现一些废水中的荧光物增加,因而提出(1)生成新的与DOM生物降解有关的荧光物质和(2)降解某些有能力抑制DOM荧光物的有机物。根据荧光物强度和UV254的比值,描述了比其他UV吸收成分发光的DOM成分的不同的生物降解动态。总而言之,大约一半的总的DOM很容易降解,剩余的DOM的浓度在8.10毫克/升之间。灌溉土壤的废水中残留的DOM浓度的升高可能有助于地下水中污染物的DOM的聚集。 展开更多
关键词 废水 溶解有机物(DOM) 激发排出物(EEM) 生物降解 Qishon(Kishon)
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Light curve inversion of asteroid(585)Bilkis with Lommel-Seeliger ellipsoid method
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作者 Ao Wang Xiao-Bin Wang +2 位作者 Karri Muinonen Xianming L.Han Yi-Bo Wang 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2016年第12期19-26,共8页
The basic physical parameters of asteroids, such as spin parameters, shape and scattering parameters, can provide us with information on the formation and evolution of both the asteroids themselves and the entire sola... The basic physical parameters of asteroids, such as spin parameters, shape and scattering parameters, can provide us with information on the formation and evolution of both the asteroids themselves and the entire solar system. In a majority of asteroids, the disk-integrated photometry measurement constitutes the primary source of the above knowledge. In the present paper, newly observed photometric data and existing data on(585) Bilkis are analyzed based on a Lommel-Seeliger ellipsoid model. With a Markov chain Monte Carlo(MCMC) method, we have determined the spin parameters(period, pole orientation)and shape(b/a, c/a) of(585) Bilkis and their uncertainties. As a result, we obtained a rotational period of 8.5738209 h with an uncertainty of 9×10^-7h, and derived a pole of(136.46°, 29.0°) in the ecliptic frame of J2000.0 with uncertainties of 0.67°and 1.1°in longitude and latitude respectively. We also derived triaxial ratios b/a and c/a of(585) Bilkis as 0.736 and 0.70 with uncertainties of 0.003 and 0.03 respectively. 展开更多
关键词 asteroids -- photometric observation -- spin parameter -- shape -- MCMC method
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二氧化碳地质储存渗漏对淡水资源的潜在风险——间歇反应试验
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作者 Jiemin Lu 葛秀珍(翻译) 何雪洲(校对) 《水文地质工程地质技术方法动态》 2011年第1期17-27,共11页
在评价广泛进行的二氧化碳地质储存可行性时,对二氧化碳渗漏到储存间隔以上地下水源的失误或意外事件的潜在件后果进行首次评估非常谨慎。为设计地下水脆测项日,需要二氧化碳介入到地下水系统的敏感性资料。进行实验室的间歇试验足为... 在评价广泛进行的二氧化碳地质储存可行性时,对二氧化碳渗漏到储存间隔以上地下水源的失误或意外事件的潜在件后果进行首次评估非常谨慎。为设计地下水脆测项日,需要二氧化碳介入到地下水系统的敏感性资料。进行实验室的间歇试验足为了探测二氧化碳对美国墨西哥湾沿岸典型含水层地下水质的影响范围。结果表明,二氧化碳在数小时或数天之内,可以增加许多阳离予的浓度。根据它们的浓度趋势,分辨出两种类型的阳离子:第一种类型的阳离子是Ca、Mg、Si、K、Sr、Mn、Ba、Co、B、Zn,这种类型的阳离了往间歇试验之前或者结束时可以快速增加下述的二氧化碳初始通量,并达到稳定浓度。第二种类型的阳离子是Fe、Al、Mo、U、V、As、Cr、Cs、Rb、Ni和Cu,这砦离子浓度在二氧化碳通量起点增加,但是,在多数的实例中都比以前的二氧化碳浓度低。白云石和方解石分解导致Ca、Mg、Mn、Ba和Sr的浓度大幅增高。在矿物质缓冲期间,由于PH值增加,阳离予释放速率呈线性降低。试验结果指出,碳酸盐矿物对地下水质变化起主要作用。地下水降解的潜征风险评价和监测策略应该主要针对这些快速起反应的矿物。而第二种阳离子移动的风险可能会自动缓解,因为PH值吲弹时,出现吸附现象。 展开更多
关键词 碳储存 二氧化碳-岩石-水的作用 碳酸盐分解 水污染 地下水临测
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Search for possible exomoons with the FAST telescope
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作者 Dragan V.Luki 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2017年第12期27-30,共4页
Our knowledge of the solar system encourages us to believe that we might expect exomoons to be present around some known exoplanets. With present hardware and existing optical astronomy methods, we do not expect to be... Our knowledge of the solar system encourages us to believe that we might expect exomoons to be present around some known exoplanets. With present hardware and existing optical astronomy methods, we do not expect to be able to find exomoons for at least 10 years, and even then, it will be a hard task to detect them. Using data from the Exoplanet Orbit Database(EOD) we find stars with Jovian exoplanets within 50 light years. Most of them will be fully accessible by the new radio telescope, the Five-hundred-meter Aperture Spherical radio Telescope(FAST), under construction which is now in the test phase. We suggest radio astronomy based methods to search for possible exomoons around two exoplanets. 展开更多
关键词 exoplanets:general exoplanets:radioastronomy exomoons
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A new ichnotaxon of fossil bird track from the Early Cretaceous Tianjialou Formation (Barremian- Albian), Shandong Province, China 被引量:11
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作者 LIRihui MartinGLockley LIUMingwei 《Chinese Science Bulletin》 SCIE EI CAS 2005年第11期1149-1154,共6页
A single set of 5 bird tracks was discovered in the Tianjialou Formation (Barremian-Albian) of Junan County, Shandong Province, China. After careful study we infer that it is a new type of bird tracks, and a new ichno... A single set of 5 bird tracks was discovered in the Tianjialou Formation (Barremian-Albian) of Junan County, Shandong Province, China. After careful study we infer that it is a new type of bird tracks, and a new ichnoge- nus was erected and the name Shandongornipes was given. The characteristics of the new bird track are as follows: Moderately large tetradactyl footprints with well-developed hallux impression. Track width (about 5.8 cm) almost equal to track length (excluding hallux); total length of track (in- cluding hallux) 8.7 cm. The digit impressions are slender, with distal ends showing long sharp claw marks. Hallux pos- teriorly-directed, and hallux impressions well-developed. Digits II, III and IV point anteriorly, exhibiting mesaxonic asymmetry: divarication angle between II and III much lar- ger than that of III and IV, the former ranging from 91° to 118°, and the latter less than 30°. Divarication between digits II and IV ranging from 128° to 142°. Without web structures. Digital pads recognizable, usually at least three in digits III and IV, but sometimes only two due to preservation. The trackway shows slight positive (inward) rotation of feet. The bird tracks occur in association with abundant dinosaur tracks, and are believed to have formed in flood plain environments. 展开更多
关键词 四趾鸟类足迹 鸟类化石 白垩纪时代 中国 山东
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Application of a fourth-order relaxation scheme to hyperbolic systems of conservation laws 被引量:7
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作者 Jianzhong Chen Zhongke Shi 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2006年第1期84-92,共9页
A fourth-order relaxation scheme is derived and applied to hyperbolic systems of conservation laws in one and two space dimensions. The scheme is based on a fourthorder central weighted essentially nonoscillatory (CW... A fourth-order relaxation scheme is derived and applied to hyperbolic systems of conservation laws in one and two space dimensions. The scheme is based on a fourthorder central weighted essentially nonoscillatory (CWENO) reconstruction for one-dimensional cases, which is generalized to two-dimensional cases by the dimension-by-dimension approach. The large stability domain Runge-Kutta-type solver ROCK4 is used for time integration. The resulting method requires neither the use of Riemann solvers nor the computation of Jacobians and therefore it enjoys the main advantage of the relaxation schemes. The high accuracy and high-resolution properties of the present method are demonstrated in one- and two-dimensional numerical experiments. 展开更多
关键词 Hyperbolic systems of conservation laws Relaxation schemes CWENO reconstruction
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Investigation of the thermodynamic processes of a floe-lead system in the central Arctic during later summer 被引量:4
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作者 LEI RuiBo LI ZhiJun +2 位作者 CHENG Bin YANG QingHua LI Na 《Chinese Journal of Polar Science》 2011年第1期10-16,共7页
Thermodynamic processes of a system involving a floe and a small lead in the central Arctic were investigated during the ice-camp period of the third Chinese National Arctic Research Expedition from 20 to 28 August, 2... Thermodynamic processes of a system involving a floe and a small lead in the central Arctic were investigated during the ice-camp period of the third Chinese National Arctic Research Expedition from 20 to 28 August, 2008. The measurements included surface air temperatures above the floe, spectral albedo of the lead, seawater temperatures in the lead and under the ice cover, and the lateral and bottom mass balance of the floe. The surface air temperature at 1.15 m remained below 0~Cthroughout the observation period and sea ice had commenced its annual cycle of growth in response to autumn cooling during the study. The surface of the lead was frozen by 23 August, after which the spectral albedo of the thin-ice-covered lead in the band of 320-950 nm was 0.46 -0.03, the seawater temperatures both in the lead and under the ice cover, as well as the vertical seawater-temperature gradient in the lead decreased gradually, and the oceanic heat under the ice was maintained at a low level approaching 0 W/m2. By the end of the measurement, the thickness of the investigated floe had reached its annual minimum, while the lateral of the floe was still in the melting phase, with a mean melting rate of 1.0±0.3 cm/d during the measurement, responding to an equivalent latent heat flux of 21 ±6 W/m2. The lateral melting of the floe had made a more significant contribution to the sea-ice mass balance than the surface and bottom melting in the end of August. 展开更多
关键词 Sea ice LEAD thermodynamic process TEMPERATURE thickness ARCTIC
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Evaluation of Average Wall Thickness of Organically Modified Mesoporous Silica
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作者 YanJunGONG ZhiHongLI BaoZhongDONG 《Chinese Chemical Letters》 SCIE CAS CSCD 2005年第1期139-142,共4页
The small angle X-ray scattering of organically modified MSU-X silica prepared by co-condensation of tetraethoxysilane (TEOS) and methyltriethoxysilane (MTES) show negative deviation from Debye’s theory due to the ... The small angle X-ray scattering of organically modified MSU-X silica prepared by co-condensation of tetraethoxysilane (TEOS) and methyltriethoxysilane (MTES) show negative deviation from Debye’s theory due to the existence of the organic interface layer. By exerting correction of the scattering negative deviation, Debye relation may be recovered, and the average wall thickness of the material may be evaluated. 展开更多
关键词 Organically modified mesoporous silica average wall thickness small angle X-ray scattering (SAXS).
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Disks and outflows in the S255IR area of high mass star formation from ALMA observations
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作者 Igor Zinchenko Sheng-Yuan Liu +1 位作者 Yu-Nung Su Yuan Wang 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2018年第8期41-44,共4页
We describe the general structure of the well known S255IR high mass star forming region, as revealed by our recent ALMA observations. The data indicate a physical relation exists between the major clumps SMA1 and SMA... We describe the general structure of the well known S255IR high mass star forming region, as revealed by our recent ALMA observations. The data indicate a physical relation exists between the major clumps SMA1 and SMA2. The driving source of the extended high velocity, well collimated bipolar outflow, is not the most pronounced disk-like SMA1 clump harboring a 20M⊙ young star (S255 NIRS3), as was assumed earlier. Apparently, it is the less evolved SMA2 clump, which drives the outflow and contains a compact rotating structure (probably a disk). At the same time, the SMA 1 clump drives another outflow, with a larger opening angle. The molecular line data do not show an outflow from the SMA3 clump (NIRS 1), which was suggested by IR studies of this region. 展开更多
关键词 STARS formation - stars massive - ISM clouds - ISM molecules - ISM individual (S255IR)
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Experimental Study and Heat Transfer Analysis on the Boiling of Saturated Liquid Nitrogen under Transient Pulsed Laser Irradiation
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作者 ZhaoyiDONG XiulanHUAI 《Journal of Thermal Science》 SCIE EI CAS CSCD 2005年第1期62-68,共7页
The boiling behavior of the liquid nitrogen (LN2) under the transient high heat flux urgently needs to be researched systematically. In this paper, the high power short pulse duration laser was used to heat the satura... The boiling behavior of the liquid nitrogen (LN2) under the transient high heat flux urgently needs to be researched systematically. In this paper, the high power short pulse duration laser was used to heat the saturated LN2 rapidly, and the high-speed photography aided by the spark light system was employed to take series of photos which displayed the process of LN2's boiling behavior under such conditions. Also, a special temperature measuring system was applied to record the temperature variation of the heating surface. The experiments indicated that an explosive boiling happened within LN2 by the laser heating, and a conventional boiling followed up after the newly-defined changeover time. By analyzing the temperature variation of the heating surface, it is found that the latent heat released by the crack of the bubbles in the bubble cluster induced by the explosive boiling is an important factor that greatly influences the boiling heat transfer mechanism. 展开更多
关键词 explosive boiling pulsed laser heating liquid nitrogen changeover time.
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Microstructure and properties of sputtered thermal barrier coatings
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作者 YangGao LunXie +1 位作者 FengPan MengchengChen 《Journal of University of Science and Technology Beijing》 CSCD 2004年第6期524-528,共5页
A 3 kW radio frequency (RF) magnetron-sputtering unit was used to producezirconia ceramic coatings on hollow turbine blades and vanes, which had been deposited a NiCrAlYbond coat layer by cathodic arc deposition. The ... A 3 kW radio frequency (RF) magnetron-sputtering unit was used to producezirconia ceramic coatings on hollow turbine blades and vanes, which had been deposited a NiCrAlYbond coat layer by cathodic arc deposition. The NiCrAlY coating surface was shot-peened, and theresidual stress in the bond coat layer and the effects of heat treatment on the residual stress arepresented. After shot peening porosities and microgaps disappear in the NiCrAlY bond coat, the wholedepth profile is residual compressive stress. Coarseness tests show that the roughness value (Rz)decreases from 16.4 to 3.3 mu m. The microstructure and phase composition of the coatings wereinvestigated using electron probe microanalysis (EPMA) and X-ray diffraction (XRD). The results showthat the/ NiCrAlY bond coat is composed of gamma and Cr phases, and the A1_2O_3 scales are formednear the interface between the ZrO_2 ceramic layer and the NiCrAlY bond coat. No degradationoccurred to RF sputtered ceramic coatings after oxidating at 1150 deg C for 100 h, heating at 1150deg C for 5 min and then air-cooling for 500 thermal cycles. 展开更多
关键词 magnetron sputtering thermal barrier coating MICROSTRUCTURE shot peening high temperature oxidation
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