期刊文献+
共找到29篇文章
< 1 2 >
每页显示 20 50 100
Comparison of conformal and intensity-modulated techniques for simultaneous integrated boost radiotherapy of upper esophageal carcinoma 被引量:92
1
作者 Wei-HuaFu Lu-HuaWang Zong-MeiZhou Jian-RongDai Yi-MinHu Lu-JunZhao 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第8期1098-1102,共5页
AIM:To compare intensity-modulated radiotherapy (IMRT) with conformal radiotherapy (CRT) by investigating the dose profiles of primary tumors,electively treated regions,and the doses to organs at risk. METHODS:CRT and... AIM:To compare intensity-modulated radiotherapy (IMRT) with conformal radiotherapy (CRT) by investigating the dose profiles of primary tumors,electively treated regions,and the doses to organs at risk. METHODS:CRT and IMRT plans were designed for five patients with upper esophageal carcinoma.For each patient, target volumes for primary lesions (67.2 Gy) and electively treated regions (50.4 Gy) were predefined.An experienced planner manually designed one CRT plan.Four IMRT plans were generated with the same dose-volume constraints,but with different beam arrangements.Indices including dose distributions,dose volume histograms (DVHs) and conformity index were compared. RESULTS:The plans with three intensity-modulated beams were discarded because the doses to spinal cord were lager than the tolerable dose 45Gy,and the dose on areas near the skin was up to 50Gy.When the number of intensity beams increased to five,IMRT plans were better than CRT plans in terms of the dose conformity and homogeneity of targets and the dose to OARs.The dose distributions changed little when the beam number increased from five to seven and nine. CONCLUSION:IMRT is superior to CRT for the treatment of upper esophageal carcinoma with simultaneous integrated boost (SIB).Five equispaced coplanar intensity-modulated beams can produce desirable dose distributions.The primary tumor can get higher equivalent dose by SIB technique. The SIB-IMRT technique shortens the total treatment time, and is an easier,more efficient,and perhaps a less error- prone way in delivering IMRT. 展开更多
关键词 上食管癌 放射疗法 等角增强调节技术 综合治疗 影像学检查
下载PDF
Therapeutic effects and prognostic factors in three-dimensional conformal radiotherapy combined with transcatheter arterial chemoembolization for hepatocellular carcinoma 被引量:45
2
作者 De-HuaWU LiLiu Long-HuaChen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第15期2184-2189,共6页
AIM: To evaluate the therapeutic efficacy of threedimensional conformal radiotherapy (3D-CRT) combined with transcatheter arterial chernoembolization (TACE) on the patients with hepatocellular carcinoma (HCC).METHODS:... AIM: To evaluate the therapeutic efficacy of threedimensional conformal radiotherapy (3D-CRT) combined with transcatheter arterial chernoembolization (TACE) on the patients with hepatocellular carcinoma (HCC).METHODS: Between 1998 and 2001, 94 patients with HCC received 3D-CRT combined with TACE. A total 63 patients had a Okuda stage Ⅰ lesion and 31 patients had stage Ⅱ. The median tumor size was 10.7 cm (range 3.0-18 cm), and liver drrhosis was present in all the patients. There were 43 cases of class A and 51 dass B. TACE was performed using lipiodol,5-fluorouracil, cisplatin, doxorubicin hydrochloride and mitomycin, followed by gelatin sponge cubes. Fifty-nine patients received TACE only one time, while the others 2 to 3 times. 3D-CRT was started 3-4 wk after TACE. All patients were irradiated with a stereotactic body frame and received 4-8 Gy single high-dose radiation for 8-12 times at the isocenter during a period of 17-26 d (median 22 d).RESULTS: The median follow-up was 37 mo (range 10-48 mo) after diagnosis. The response rate was 90.5%. The overallsurvival rate at 1-, 2-, and 3- year was 93.6%, 53.8% and 26.0% respectively, with the median survival of 25 too. On univariate analysis, age (P=-0.026), Child-Pugh classification for cirrhosis of liver (P=0.010), Okuda stage (P=-0.026),tumor size (P=0.000), tumor type (P=0.029), albuminemia (P=0.035), and radiation dose (P=0.000) proved to be significant factors for survival. On multivariate analysis,age (P=-0.024), radiation dose(P=-0.001), and tumor size (P=0.000) were the significant factors.CONCLUSION: 3D-CRT combined with TACE is an effective and feasible approach for HCC. Age, radiation dose and tumor size were found to be significant prognostic factors for survival of patients with HCC treated by 3D-CRT combined with TACE. Further study for HCC is needed to improve the treatment efficacy. 展开更多
关键词 治疗作用 预兆因素 3D-CRT 三维放射线疗法 输导管 肝细胞癌 肿瘤 动脉 HCC
下载PDF
KAI1 gene expression in colonic carcinoma and its clinical significances 被引量:18
3
作者 De-HuaWu LiLiu +1 位作者 Long-HuaChen Yan-QingDing 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第15期2245-2249,共5页
AIM: To investigate KAI1 gene expression in the progression of human colonic carcinoma and its clinical significances.METHODS: KAI1 expression was detected by in situ hybridization and immunohistochemistry in the 4 es... AIM: To investigate KAI1 gene expression in the progression of human colonic carcinoma and its clinical significances.METHODS: KAI1 expression was detected by in situ hybridization and immunohistochemistry in the 4 established cell lines of colorectal carcinoma with different metastatic potentials, and in 80 specimens of colonic carcinoma, 21 colonic carcinoma specimens with lymphatic metastasis and 20 controls of normal colonic mucosa.RESULTS: The expressions of KAI1 in HT29 and SW480 cell lines were higher than those in LoVo and SW620. Theexpression of KAI1 gene was significantly higher in colorectal carcinoma compared with normal colonic mucosa andlymphatic metastasis (X^2=46.838, P<0.01). The expression of KAI1 gene had no relationship with histological grade.The KAI1 expressions in Dukes A and B carcinoma were higher at both mRNA and protein levels compared to Dukes C carcinoma (72=16.061, P<0.05). The expression of KAI1 in colonic carcinoma specimens with lymphatic metastasis was almost lost. The results of in situ hybridization were in concordance with immunohistochemistry.CONCLUSION: KAI1 is highly related to the metastasis of colonic carcinoma and may be a useful indicator of metastasis in colonic carcinoma. 展开更多
关键词 KAI1基因 基因表达 结肠癌 直肠癌 临床经验 肿瘤
下载PDF
Tiaml gene expression and its significance in colorectal carcinoma 被引量:16
4
作者 LiLiu De-HuaWu Yan-QingDing 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第5期705-707,共3页
AIM: To explore the expression of Tiam1 gene in colorectal carcinoma and its correlation with tumor metastasis. METHODS: Expressions of Tiaml gene in 8 colorectal carcinoma cell lines were detected by reverse transcri... AIM: To explore the expression of Tiam1 gene in colorectal carcinoma and its correlation with tumor metastasis. METHODS: Expressions of Tiaml gene in 8 colorectal carcinoma cell lines were detected by reverse transcriptase-polymerase chain reaction. In vitro invasiveness was determined by means of Matrigel invasion assay. The correlation of Tiaml expression with the invasive ability was also analyzed. RESULTS: Tiaml gene was highly expressed in LoVo and SW620, which were established from metastatic colorectal carcinomas in comparison with LS174T, SW480, HCT116, LST, HRT-18 and Hee8693, which were established from primary colorectal carcinomas. In vitro cell invasivion demonstrated that LoVo and SW620 had a higher invasive ability than LS174T, SW480, HCT116, LST, HRT-18 and Hee8693. The expression of Tiaml gene was highly related to the metastatic potential of colorectal carcinoma cells. CONCLUSION: Tiaml gene may play an important role in invasion and metastasis of colorectal carcinoma and is a metastasis-related gene. 展开更多
关键词 Colorectal carcinoma Tiam1 gene Gene expression Tumor metastasis
下载PDF
Impact of simultaneous assay, the PCNA, cyclinDl, and DNA content with specimens before and after preoperative radiotherapy on prognosis of esophageal cancer-possible incorporation into clinical TNM staging system 被引量:17
5
作者 Shu-ChaiZhu RenLi Yu-XiangWang WeiFeng JuanLi RongQiu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第25期3823-3829,共7页
AIM: The aim of the present study is to use immunohisto chemical methods to investigate the clinical implications of tumor markers in esophageal squamous cell carcinoma and evaluate their impact on prognosis. METHODS:... AIM: The aim of the present study is to use immunohisto chemical methods to investigate the clinical implications of tumor markers in esophageal squamous cell carcinoma and evaluate their impact on prognosis. METHODS: From November 1990 to December 1996, 47 patients were treated with preoperative radiation followed by radical esophagectomy. All patients were confirmed pathologically as suffering from squamous cell carcinoma. Immunohistochemical stain was done for PCNA, cyclinDl protein expression and DNA content analyzed by image cytometry. Kaplan-Meier method for single prognostic factor and log-rank test was used to test the significant difference. Cox stepwise regression model and prognosis index model were used for survival analysis with multiple prognostic factors. RESULTS: Radio-pathological change, T stage and N stage, as the traditional prognostic factors had statistical difference in 3-, 5- and 10-year survival rates. While, tumor cell proliferating marked PCNA, cyclinDl and DNA content served as independent prognostic factors of esophageal carcinoma. There was definitely an identity between the single and multiple factor analyses. PI was more accurate to evaluate the prognosis of esophageal carcinoma. CONCLUSION: It is possible that tumor cell proliferating marked PCNA, cyclinD1 and DNA content would become the endpoints for evaluating the prognosis of esophageal carcinoma. 展开更多
关键词 Esophageal carcinoma RADIOTHERAPY Cell proliferating marker
下载PDF
一种新的高转移膀胱癌动物模型的建立 被引量:14
6
作者 李凌 武文森 +7 位作者 徐萍 王丰 谢匡成 陈霞芳 易苗英 顾青 李川源 黄倩 《中华实验外科杂志》 CAS CSCD 北大核心 2002年第1期78-79,共2页
目的 建立一种简便、可靠的小鼠膀胱癌模型。方法 将绿色荧光蛋白 (GFP)表达质粒导入小鼠膀胱癌细胞株BTT T73 9,G418筛选稳定表达GFP的克隆。然后接种于同系小鼠膀胱壁内及后腿皮下 ,荧光显微镜直接观察肿瘤细胞生长、浸润与微转移 ... 目的 建立一种简便、可靠的小鼠膀胱癌模型。方法 将绿色荧光蛋白 (GFP)表达质粒导入小鼠膀胱癌细胞株BTT T73 9,G418筛选稳定表达GFP的克隆。然后接种于同系小鼠膀胱壁内及后腿皮下 ,荧光显微镜直接观察肿瘤细胞生长、浸润与微转移 ,流式细胞术定量分析转移的肿瘤细胞数 ,并与常规石蜡切片苏木素 伊红 (HE)染色比较。结果 经GFP标记的肿瘤细胞在荧光显微镜下发出绿色荧光 ,膀胱原位接种 (2~ 4)× 10 5后 1周即出现膀胱肿大、血尿 ,以后膀胱肿大加重、出现输尿管阻塞、肾盂积水 ,以及肾、腹腔、肺、肝、脾等远处转移。结论 GFP标记的BTT T73 9膀胱癌细胞和膀胱癌动物模型可十分简便、准确判断膀胱癌肿瘤细胞生长、浸润与微转移等。 展开更多
关键词 膀胱肿瘤 肿瘤转移 动物模型 绿色荧光蛋白
原文传递
Effects of KAI1/CD82 on biological behavior of human colorectal carcinoma cell line 被引量:15
7
作者 LiLiu De-HuaWu +2 位作者 Zu-GuoLi Guang-ZhiYang Yan-QingDing 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第6期1231-1236,共6页
AIM: To investigate the effects of KAI1/CD82 on biological behavior of colorectal carcinoma cells. METHODS: KAI1 cDNA was transfected into highly malignant colorectal carcinoma cell line, LoVo, which had low level of ... AIM: To investigate the effects of KAI1/CD82 on biological behavior of colorectal carcinoma cells. METHODS: KAI1 cDNA was transfected into highly malignant colorectal carcinoma cell line, LoVo, which had low level of endogenous KAI1 expression, and established stable transfectant clones with high KAI1/CD82 expression.The cell-cell adhesion, cell aggregation, cell-matrix adhesion and cell invasion assay were performed to determine whether KAI1 transfectant could have an effect on proliferation,adhesion and tumor metastasis in comparison with the control transfectant cells. RESULTS: KAI1 expression did not alter in vitro cell proliferation. But the KAI1 transfectant cells exhibited significantly increased homotypic cell-cell adhesion and cell aggregation in comparison with the control transfectant cells (P<0.05). Furthermore, KAI1 expression significantly suppressed the cell adhesion to extracellular matrix components and in vitro cell invasion in KAI1-transfected LoVo cells. The data indicated that KAI1 expression significantly suppressed the metastatic potential of KAI1-transfected LoVo cells. CONCLUSION: Our results suggest that KAI1 might function as a negative regulator of colorectal carcinoma metastasis. 展开更多
关键词 结肠癌 直肠癌 KAI1 肿瘤转移
下载PDF
Antitumor effects and radiosensitization of cytosine deaminase and thymidine kinase fusion suicide gene on colorectal carcinoma cells 被引量:11
8
作者 De-HuaWu LiLiu Long-HuaChen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第20期3051-3055,共5页
AIM: To investigate the killing effect and radiosensitization of double suicide gene mediated by adenovirus on colorectal carcinoma cells. METHODS: Colorectal carcinoma cell line SW480 was transfected with adenovirus ... AIM: To investigate the killing effect and radiosensitization of double suicide gene mediated by adenovirus on colorectal carcinoma cells. METHODS: Colorectal carcinoma cell line SW480 was transfected with adenovirus expression vector containing cytosine deaminase (CD) and thymidine kinase (TK) fusion gene. The expression of CD-TK fusion gene was detected by reverse transcriptase-polymerase chain reaction. The toxic effect of ganciclovir (GCV) and 5-fluorocytosine (5-FC) on infected cells was determined by MTT assay. The radiosensitization of double suicide gene was evaluated by clonogenic assay. RESULTS: After prodrugs were used, the survival rate of colorectal carcinoma cells was markedly decreased. When GCV and 5-FC were used in combination, the cytotoxicity and bystander effect were markedly superior to a single prodrug (X2 = 30.371, P<0.01). Both GCV and 5-FC could sensitize colorectal carcinoma cells to the toxic effect of radiation, and greater radiosensitization was achieved when both prodrug were used in combination. CONCLUSION: CD-TK double suicide gene can kill and radiosensitize colorectal carcinoma cells. 展开更多
关键词 CD-TK Suicide gene RADIOSENSITIZATION Colorectal carcinoma
下载PDF
电穿孔介导质粒DNA肿瘤内转移抑制恶性肿瘤生长与转移 被引量:11
9
作者 王丰 陈霞芳 +4 位作者 田毓华 吴继红 李凌 李川源 黄倩 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2002年第5期734-740,共7页
利用携带绿色荧光蛋白 (greenfluorescentprotein ,GFP)编码基因的表达质粒 ,测试电穿孔方法介导目的基因活体组织内转移的效率并优化电击参数 .在此基础上采用电穿孔技术直接将编码白介素 12 (IL 12 )、白介素 2 (IL 2 )、粒单细胞克... 利用携带绿色荧光蛋白 (greenfluorescentprotein ,GFP)编码基因的表达质粒 ,测试电穿孔方法介导目的基因活体组织内转移的效率并优化电击参数 .在此基础上采用电穿孔技术直接将编码白介素 12 (IL 12 )、白介素 2 (IL 2 )、粒单细胞克隆刺激因子 (GM CSF)等免疫调节因子或反义血管内皮细胞生长因子 12 1(VEGF12 1)、可溶性血管内皮细胞膜受体 (sFlk 1及ExTek)等血管生成抑制因子表达质粒转移至肿瘤局部 .实验结果表明电穿孔介导GFP表达质粒肌肉内转移的效率较高 ,GFP可在肌细胞内持续高水平表达 3周以上 ,而在肿瘤细胞内只能表达 4~ 6d ,但高电压短脉冲电击组肿瘤内GFP阳性细胞数比低电压长脉冲组高 2 6 8倍 .多次电击介导IL 12表达质粒转移至肿瘤组织内 ,可有效地抑制小鼠膀胱癌BTT gfp、人乳腺癌MCF 7及肝癌SMMC 772 1 gfp的生长 .MCF 7对血管生成抑制因子基因转移治疗较敏感 ,单独应用反义VEGF12 1、sFlk 1或ExTek即显示明确的治疗效果 .SMMC 772 1 gfp单独应用sFlk 1有效 .小鼠膀胱癌对单独应用反义VEGF12 1、sFlk 1或ExTek治疗效果不理想 ,但联合应用sFlk 1和ExTek仍然可以有效地抑制肿瘤生长与转移 ,甚至使肿瘤缩小或消失 .提示电穿孔技术是一项高效、安全。 展开更多
关键词 电穿孔 质粒DNA 肿瘤内转移 抑制 恶性肿瘤生长 基因治疗 免疫调节 血管生成 肿瘤转移
下载PDF
Docetaxel inhibits SMMC-7721 human hepatocellular carcinoma cells growth and induces apoptosis 被引量:13
10
作者 Chang-XinGeng Zhao-ChongZeng Ji-YaoWang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第4期696-700,共5页
AIM: To investigate the in vitro anti-hepatocellular carcinoma (HCC) activity of docetaxel against SMMC-7721 HCC cells and its possible mechanism.METHODS: The HCC cells were given different concentrations of docetaxel... AIM: To investigate the in vitro anti-hepatocellular carcinoma (HCC) activity of docetaxel against SMMC-7721 HCC cells and its possible mechanism.METHODS: The HCC cells were given different concentrations of docetaxel and their growth was measured by colony forming assay. Cell cycle and apoptosis were analyzed by flow cytometry and fluorescence microscopy (acridine orange/ethidium bromide double staining, AO/EB), as well as electronic microscopy. The SMMC-7721 HCC cell reactive oxygen species (ROS) and glutathione (GSH) were measured after given docetaxel.RESULTS: Docetaxel inhibited the hepatocellular carcinoma cells growth in a concentration dependent manner with IC505×10-10 M. Marked cell apoptosis and G2/M phase arrest were observed after treatment with docetaxel ≥10-8M.Docetaxel promoted SMMC-7721 HCC cells ROS generation and GSH deletion.CONCLUSION: Docetaxel suppressed the growth of SMMC7721 HCC cells in vitro by causing apoptosis and G2/M phase arrest of the human hepatoma cells, and ROS and GSH may play a key role in the inhibition of growth and induction of apoptosis. 展开更多
关键词 多舍他西 肝细胞癌 化疗 细胞凋亡 SMMC-7721细胞系
下载PDF
Effect of intraoperative radiotherapy combined with external beam radiotherapy following internal drainage for advanced pancreatic carcinoma 被引量:12
11
作者 Hong-BingMa Zheng-LiDi +3 位作者 Xi-JingWang Hua-FenKang Huai-CiDeng Ming-HuaBai 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第11期1669-1671,共3页
AIM: To determine the survival of advanced pancreatic cancer patients treated with intraoperative radiotherapy (IORT) combined with external beam radiation therapy (EBRT) following internal drainage (cholecystojejunos... AIM: To determine the survival of advanced pancreatic cancer patients treated with intraoperative radiotherapy (IORT) combined with external beam radiation therapy (EBRT) following internal drainage (cholecystojejunostomy or choledochojejunostomy). METHODS: Eighty-one patients with advanced pancreatic cancer who received IORT combined with EBRT following internal drainage (ID) between 1996 and 2001 were retrospectively analyzed. Among the 81 patients, 18 underwent ID+IORT, 25 ID+IORT+EBRT (meanwhile, given 5-Fu 300 mg/m^2 iv drip, 2f/w), 16 EBRT, 22 had undergone simple internal drainage. The IORT dose was 15-25Gy in a single fraction. The usual EBRT dose was 30-40Gy with a daily fraction of 1.8-2.0 Gy. RESULTS: The complete remission rate, partial remission rate of patients with backache and abdominal pain treated with ID+IORT were 55.5%, 33.3% respectively. Alleviation of pain was observed 2 or 3 wk after IORT. The median survival time (MST) of ID+IORT group was 10.7 mo. The pain remission rate of patients treated with ID+IORT+EBRT was 92%, and their MST was 12.2 mo. The MST of patients treated with EBRT and simple internal drainage was 5.1 mo and 7.0 mo, respectively. The survival curve of ID+IORT group and ID+IORT+EBRT group was significantly better than that of EBRT group (P<0.05). The difference between the ID+IORT+EBRT group and ID group was significant (P<0.05). CONCLUSION: IORT combined with EBRT following internal drainage can alleviate pain, improve quality of life and prolong survival time of patients with advanced pancreatic cancer. 展开更多
关键词 外科手术 放射线疗法 胰腺癌 晚期 IORT EBRT 物理治疗
下载PDF
Human γδT-cell subsets and their involvement in tumor mmunity 被引量:12
12
作者 Dang W u Pin Wu +2 位作者 Fuming Qiu Qichun Wei Jian Huang 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2017年第3期245-253,共9页
γδT cells are a conserved population of innate lymphocytes with diverse structural and functional heterogeneity that participate in various immune responses during tumor progression. γδT cells perform potent immun... γδT cells are a conserved population of innate lymphocytes with diverse structural and functional heterogeneity that participate in various immune responses during tumor progression. γδT cells perform potent immunosurveillance by exerting direct cytotoxicity, strong cytokine production and indirect antitumor immune responses. However, certain γδT-cell subsets also contribute to tumor progression by facilitating cancer-related inflammation and immunosuppression. Here, we review recent observations regarding the antitumor and protumor roles of major structural and functional subsets of human γδT cells, describing how these subsets are activated and polarized, and how these events relate to subsequent function in tumor immunity. These studies provide insights into the manipulation of γδT-cell function to facilitate more targeted approaches for tumor therapy. 展开更多
关键词 ANTITUMOR γδT cells protumor SUBSETS tumor immunity
原文传递
早期下咽癌手术和(或)放射治疗的临床研究 被引量:9
13
作者 祁超 吴维平 +1 位作者 崔可飞 F.Baillet 《中华放射肿瘤学杂志》 CSCD 北大核心 2000年第4期229-231,共3页
目的 比较 132例T1、T2 期下咽癌患者手术 +放射治疗与单纯放射治疗的疗效。方法  132例T1、T2 期下咽癌患者中 ,5 1.5 %肿瘤直径在 2~ 4cm范围内 ,83.3%来源于梨状窝。颈部淋巴结阴性 (N0 )者占总数的 5 0 %。治疗分为 3个组 ,即部... 目的 比较 132例T1、T2 期下咽癌患者手术 +放射治疗与单纯放射治疗的疗效。方法  132例T1、T2 期下咽癌患者中 ,5 1.5 %肿瘤直径在 2~ 4cm范围内 ,83.3%来源于梨状窝。颈部淋巴结阴性 (N0 )者占总数的 5 0 %。治疗分为 3个组 ,即部分咽喉切除术 +术后放射治疗 (PPL +RX)组 (4 4例 ) ,全咽喉切除术 +术后放射治疗 (TPL +RX)组 (4 0例 ) ,单纯放射治疗 (RX)组 (4 8例 )。术后放射治疗原发灶区和 (或 )颈淋巴引流区的放射剂量为 45~ 5 5Gy。单纯放射治疗的照射剂量原发灶区为 75Gy,颈淋巴引流区为 45~ 5 5Gy ,颈转移淋巴结处总量达 75Gy。生存率计算采用Kaplan Meier法 ,显著性检验采用Logrank法。结果 总 1、3、5年生存率分别为 71%、45 %、34%。单纯放射治疗组与术后放射治疗组间的 1、3、5年生存率比较 ,后者治疗效果均优于前者 (PPL +RX与RX :χ2 =7.0 1,P <0 .0 1;TPL +RX与RX :χ2 =4.0 1,P <0 .0 5 )。咽喉全切除术或部分切除 +术后放射治疗组间的 1、3、5年生存率差异无显著性意义 (χ2 =1.85 ,P >0 .10 )。N0 者行颈清扫 +术后放射治疗与单纯放射治疗的颈淋巴区的控制率差异也无显著性意义 (χ2 =1.96 ,P >0 .10 ) ,但术前颈淋巴结有临床转移者 (N+)则颈清扫具有明显优势 (χ2 =12 .2 1。 展开更多
关键词 下咽癌 综合治疗 放射治疗 外科手术
原文传递
Patients with brain metastases from gastrointestinal tract cancer treated with whole brain radiation therapy:Prognostic factors and survival 被引量:10
14
作者 SusanneBartelt FelixMomm ChristianWeissenberger JohannesLutterbach 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第22期3345-3348,共4页
AIM: To identify the prognostic factors with regard to survival for patients with brain metastasis from primary tumors of the gastrointestinal tract. METHODS: Nine hundred and sixteen patients with brain metastases, t... AIM: To identify the prognostic factors with regard to survival for patients with brain metastasis from primary tumors of the gastrointestinal tract. METHODS: Nine hundred and sixteen patients with brain metastases, treated with whole brain radiation therapy (WBRT) between January 1985 and December 2000 at the Department of Radiation Oncology, University Hospital Freiburg, were analyzed retrospectively. RESULTS: Fifty-seven patients presented with a primary tumor of the gastrointestinal tract (esophagus: n=0, stomach: n=10, colorectal: n=47). Twenty-six patients had a solitary brain metastasis, 31 patients presented with multiple brain metastases. Surgical resection was performed in 25 patients. WBRT was applied with daily fractions of 2 Gray (Gy) or 3Gy to a total dose of 50Gy or 30Gy, respectively. The interval between diagnoses of the primary tumors and brain metastases was 22.6mo vs 8.0mo for patients with primary tumors of the colon/rectum vs other primary tumors, respectively (P<0.01, log-rank). Median overall survival for all patients with brain metastases (n=916) was 3.4mo and 3.2mo for patients with gastrointestinal neoplasms. Patients with gastrointestinal primary tumors presented significantly more often with a solitary brain metastasis than patients with other primary tumors (P<0.05, log-rank). In patients with gastrointestinal neoplasms (n=57), the median overall survival was 5.8 mo for patients with solitary brain metastasis vs 2.7mo for patients with multiple brain metastases (P<0.01, log-rank). The median overall survival for patients with a Karnofsky performance status (KPS) ≥70 was 5.5mo vs 2.1mo for patients with KPS <70 (P<0.01, log-rank). At multivariate analysis (Cox Model) the performance status and the number of brain metastases were identified as independent prognostic factors for overall survival. CONCLUSION: Brain metastases occur late in the course of gastrointestinal tumors. Pretherapeutic variables like KPS and the number of brain metastases have a profound influence on treatment o 展开更多
关键词 ADULT Aged Aged 80 and over Brain Neoplasms FEMALE Gastrointestinal Neoplasms Humans MALE Middle Aged Prognosis Retrospective Studies Risk Factors Survival Analysis
下载PDF
Cytokine and Immuno-Gene Therapy for Solid Tumors 被引量:10
15
作者 Chuan-YuanLi QianHuang Hsiang-FuKung 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2005年第2期81-91,共11页
Despite recent progress in our understanding of cancer biology and in many areas of cancer treatment,the success rate for cancer therapy remains dismal.Immunotherapy for cancer has long been an exciting field for many... Despite recent progress in our understanding of cancer biology and in many areas of cancer treatment,the success rate for cancer therapy remains dismal.Immunotherapy for cancer has long been an exciting field for many cancer researchers due to the possibility to mobilize the body's own immune system to eradicate cancer not only locally but also systemically.Since its initial discovery,cytokine-based immunotherapy has been vigorously and extensively investigated for cancer treatment due to the perception of it as a relatively easily purifiable,injectable form of cancer treatment agent.However,so far most cytokine-based therapy trials have fallen short of expectations.One of main obstacles is the difficulty to achieve therapeutically relevant dosage in patients without generating excessive normal tissue toxicity.The emergence of novel gene therapy approach to deliver therapeutic cytokine to tumors locally generated great excitement since it has the potential of generating sustained high local concentration of immunostimulatory cytokine without raising the systemic levels of the cytokines,which is responsible for most of the observed toxicity.In this review,we will attempt to provide an overview of the field and discuss some of the problems associated with cytokine-based immuno-gene therapy and potential solutions. Cellular & Molecular Immunology.2005;2(2):81-91. 展开更多
关键词 CYTOKINE gene therapy CANCER IMMUNOTHERAPY
原文传递
Post-radiation survival time in hepatocellular carcinoma based on predictors for CT-determined, transarterial embolization and various other parameters 被引量:9
16
作者 Ting-KaiLeung Chi-MingLee +3 位作者 Li-KuoShen Hsi-ChiChen Yu-ChengKuo Jeng-FongChiou 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第11期1697-1699,共3页
AIM: In this retrospective study of unresectable hepatocellular carcinoma (HCC), we have investigated the efficacy of CT-derived parameters, laboratory measurements, clinical assessment and associated transarterial em... AIM: In this retrospective study of unresectable hepatocellular carcinoma (HCC), we have investigated the efficacy of CT-derived parameters, laboratory measurements, clinical assessment and associated transarterial embolization (TAE) as predictors of post-radiotherapy survival time. METHODS: Sixty-six patients diagnosed with unresectable HCC that had undergone radiotherapy at two medical university hospitals in Taipei were enrolled in the study. Using multivariant analysis, pre-treatment parameters including tumor number and CT confirmation of PVT and ascites were compared. Multivariant analysis was also used for comparison of the mean pretreatment values for laboratory measurements, including alpha-fetoprotein, direct/total bilirubin and GOT/GPT levels, and clinical history of chronic hepatitis across the three survival-time categories. The x2 was used to test the significance of the relationship between survival time and TAE procedure. The P values for the above tests were deemed statistically significant where P<0.05. RESULTS: Portal vein thrombosis (P= 0.032) and ascites (P><0.05) were negative predictors of post-radiation survival time. Low-grade liver cirrhosis (A or B), lower tumor volume and low levels of AFT, GOT/GPT, and total bilirubin were predictors of longer post-radiation survival time (P<0.05). CONCLUSION: The CT and clinical and laboratory assessment provide a reference for, and enable estimation of, probable survival times in HCC patients after radiotherapy. Tumor volume, severity of liver cirrhosis, status with respect to portal vein thrombosis and ascites and AFT, GOT/GPT and total bilirubin values were significant predictors of survival in this study. 展开更多
关键词 Hepatocellular carcinoma HCC Radiotherapy CT-determined parameters Transarterial embolization Post-radiation survival time
下载PDF
Intensity modulated radiation therapy and chemotherapy for Ioca advanced pancreatic cancer:Results of feasibility study 被引量:5
17
作者 Yong-RuiBai Guo-HuaWu +5 位作者 Wei-JianGuo Xu-GongWu YuanYao YinChen Ren-HuaZhou Dong-QinLu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第11期2561-2564,共4页
AIM: To explore whether intensity modulated radiation therapy (IMRT) in combination with chemotherapy could increase radiation dose to gross tumor volume without severe acute radiation related toxicity by decreasing t... AIM: To explore whether intensity modulated radiation therapy (IMRT) in combination with chemotherapy could increase radiation dose to gross tumor volume without severe acute radiation related toxicity by decreasing the dose to the surrounding normal tissue in patients with locally advanced pancreatic cancer.METHODS: Twenty-one patients with locally advanced pancreatic cancer were evaluated in this clinical trial,Patients would receive the dose of IMRT from 21Gy to 30Gy in 7 to 10 fractions within two weeks after conventional radiotherapy of 30Gy in 15 fractions over 3 weeks. The total escalation tumor dose would be 51, 54,57, 60Gy, respectively. 5-fluororacil (5-FU) or gemcitabine was given concurrently with radiotherapy during the treatment course.RESULTS: Sixteen patients who had completed the radiotherapy plan with doses of 51Gy (3 cases), 54Gy (3 cases), 57Gy (3 cases) and 60Gy (7 cases) were included for evaluation. The median levels of CA19-9 prior to and after radiotherapy were 716 U/ml and 255 U/ml respectively (P<0.001) in 13 patients who demonstrated high levels of CA19-9 before radiotherapy. Fourteen patients who suffered from pain could reduce at least 1/3-1/2 amount of analgesic intake and 5 among these patients got complete relief of pain. Ten patients improved in Kamofsky performance status (KPS). The median follow-up period was 8 months and one-year survival rate was 35 %. No patient suffered more than grade Ⅲ acute toxicities induced by radiotherapy.CONCLUSION: Sixty Gy in 25 fractions over 5 weeks with late course IMRT technique combined with concurrent 5-FU chemotherapy can provide a definitely palliative benefit with tolerable acute radiation related toxicity for patients with advanced pancreatic cancer. 展开更多
关键词 增强放射疗法 化学疗法 局限性胰腺癌 进展期 可行性 临床疗效
下载PDF
Expression of a novel immunoglobulin gene SNC73 in human cancer and non-cancerous tissues 被引量:5
18
作者 Jian-BinHu ShuZheng Yong-ChuanDeng 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第5期1054-1057,共4页
AIM: To investigate the expression of immunoglobulin gene SNC73 in malignant tumors and non-cancerous normal tissues.METHODS: Expression level of SNC73 in tumors and non-cancerous tissues from the same patient was det... AIM: To investigate the expression of immunoglobulin gene SNC73 in malignant tumors and non-cancerous normal tissues.METHODS: Expression level of SNC73 in tumors and non-cancerous tissues from the same patient was determined by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay (RT-PCR-ELISA) in 90 cases of malignant tumors, including colorectal cancer, gastric cancer, breast cancer, lung cancer and liver cancer, Analysis on the correlation of SNC73 expression with sex, age, site,grade of differentiation, depth of invasion, and metastases in colorectal cancer patients was made.RESULTS: Expression level of SNC73 in non-cancerous colorectal mucosa and colorectal cancerous tissues was 1.234±0.842 and 0.737±0.731, respectively (P<0.01), with the mean ratio of 7.134±14.092 (range, 0.36-59.54).Expression of SNC73 showed no significant difference among gastric cancer, breast cancer, lung cancer and liver cancer when compared with non-cancerous tissues (P>0.05). No correlation was found between SNC73 expression level and various clinicopathological factors, including sex, age, site,grade of differentiation, depth of invasion and metastases of CRC patients.CONCLUSION: Down-regulation of SNC73 expression may be a relatively specific phenomenon in colorectal cancer.SNC73 is a potential genetic marker for the carcinongenesis of colorectal cancer. The relationship of SNC73 expression and carcinogenesis of colorectal cancer merits further study. 展开更多
关键词 免疫球蛋白 基因表达 肿瘤 SNC73基因 聚合酶链反应 ELISA
下载PDF
利用绿色荧光蛋白动态观察活体内肿瘤细胞生长及瘤体形成过程 被引量:2
19
作者 黄倩 李川源 《中华病理学杂志》 CAS CSCD 北大核心 2000年第3期230-232,共3页
关键词 肿瘤细胞 绿色荧光蛋白 瘤体形成 肿瘤浸润
原文传递
Docetaxel shows radiosensitization in human hepatocellular carcinoma cells 被引量:3
20
作者 Chang-XinGeng Zhao-ChongZeng +2 位作者 Ji-YaoWang Shi-YingXuan Chong-MaoLin 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第19期2990-2993,共4页
AIM: To determine the radiosensitizing potential of docetaxel in human hepatocellular carcinoma SMMC-7721 cells and its mechanisms.METHODS: SMMC-7721 cells were incubated with docetaxel at 0.125, 0.25, and 0.5 nmoL/L ... AIM: To determine the radiosensitizing potential of docetaxel in human hepatocellular carcinoma SMMC-7721 cells and its mechanisms.METHODS: SMMC-7721 cells were incubated with docetaxel at 0.125, 0.25, and 0.5 nmoL/L for 24 h and at 0.125 and 0.25 nmol/L for 48 h before irradiation. Radiation doses were given from 0 to 10 Gy. Cell survival was measured by a standard clonogenic assay after a 9-d incubation. The reactive oxygen species (ROS) and glutathione (GSH) are detected after being given the same dose of docetaxel for the same time. RESULTS: The sensitization enhancement ratios (SER) for SMMC-7721 cells determined at the 50% survival level were 1.15, 1.21 and 1.49 at 0.125, 0.25, and 0.5 nmol/L for pre-incubation of 24 h, respectively; the SER were 1.42, 1.67 at 0.125 and 0.25 nmol/L, for pre-incubation of 48 h, respectively. The ROS of SMMC-7721 cells increased and GSH decreased after pretreatment with the same doses of docetaxel for 24 or 48 h.CONCLUSION: A radiosensitizing effect of docetaxel could be demonstrated unambiguously in this cell line used. In addition, our data showed that the mechanism of radiopotentiation by docetaxel probably does not involve a G2/M block in SMMC-7721 cells, and ROS generation and GSH deletion may play a key role in the radiosensitizing effect of docetaxel. 展开更多
关键词 DOCETAXEL Hepatocellular carcinoma SMMC-7721cell line RADIOSENSITIZATION Reactive oxygen species
下载PDF
上一页 1 2 下一页 到第
使用帮助 返回顶部