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Genetic parameter estimates for growth traits at early stage of Pacific abalone,Haliotis discus hannai Ino 被引量:13
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作者 DENG Yuewen LIU Xiao +1 位作者 ZHANG Guofan ZHAO Hongen 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2007年第5期90-95,共6页
Heritability and genetic and phenotypic correlations were estimated for juvenile growth traits of Pacific abalone Haliotis discus hannai Ino. The estimates were calculated from shell length and shell width measurement... Heritability and genetic and phenotypic correlations were estimated for juvenile growth traits of Pacific abalone Haliotis discus hannai Ino. The estimates were calculated from shell length and shell width measurements on progeny resulting from 12 half-sib families and 36 full-sib families obtained using artificial fertilization of mating three females to each male. The measurements were taken at 10, 20 and 30 d after fertilization. It was found that heritability estimates based on sire component ranged from 0.23 to 0.36 for shell length and 0.21 to 0.32 for shell width. Heritability estimates from dam component were larger than those from sire component at three ages, indicating presence of maternal effects, non-additive genetic effects and common environmental effects. Phenotypic correlations were significant at three ages (P 〈 0.05), with values of 0.92, 0.93 and 0.92, respectively. Genetic correlations from the paternal half-sib correlation analysis were highly positive at three ages, with values of 0.50, 0.78 and 0.81, respectively. The results suggest that selective breeding is an effective approach to improving growth traits of Pacific abalone stocks. 展开更多
关键词 Haliotis discus hannai Ino growth traits HERITABILITY genetic correlations
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Cultivation of marine sponges 被引量:3
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作者 曲翊 张卫 +2 位作者 李华 虞星炬 金美芳 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2005年第2期194-198,共5页
Sponges are the most primitive of multicellular animals, and are major pharmaceutical sources of marine secondary metabolites. A wide variety of new compounds have been isolated from sponges. In order to produce suffi... Sponges are the most primitive of multicellular animals, and are major pharmaceutical sources of marine secondary metabolites. A wide variety of new compounds have been isolated from sponges. In order to produce sufficient amounts of the compounds of the needed, it is necessary to obtain large amount of sponges. The production of sponge biomass has become a focus of marine biotechnology. 展开更多
关键词 SPONGE CULTURE
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A Pt/CeO_(2)Hybrid Nanozyme with Stable Peroxidase Activity for the Detection of Acetylcholine
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作者 CUI Xulian MA Baofu +6 位作者 PAN Hui XIA Yu LIU Li ZHAO Baofeng LIANG Zhen ZHANG Lihua ZHANG Yukui 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2024年第2期268-271,共4页
Acetylcholine(ACh)is a common neurotransmitterl)that is secreted by cholinergic neurons[2]and is found mainly in the peripheral and central nervous systems.[3]ACh transmits nerve signals from presynaptic to postsynapt... Acetylcholine(ACh)is a common neurotransmitterl)that is secreted by cholinergic neurons[2]and is found mainly in the peripheral and central nervous systems.[3]ACh transmits nerve signals from presynaptic to postsynaptic cells via synapses. 展开更多
关键词 Acetylcholine/choline Nanozyme Hydrogen peroxide Alzheimer’s disease
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Current status of greenhouse gas emissions from aquaculture in China 被引量:2
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作者 Congjun Xu Guohuan Su +5 位作者 Kangshun Zhao Xiaoqi Xu Ziqi Li Qiang Hu Ying Xue Jun Xu 《Water Biology and Security》 2022年第3期10-17,共8页
Aquaculture and mariculture are becoming an increasingly important source of food supply in many countries and regions.However,with the expansion of aquaculture and mariculture comes increasing emissions of greenhouse... Aquaculture and mariculture are becoming an increasingly important source of food supply in many countries and regions.However,with the expansion of aquaculture and mariculture comes increasing emissions of greenhouse gases(GHG)which contribute to global warming and climate change.China leads the world in aquaculture and mariculture production,but there are no studies that systematically assess China's overall carbon footprint from these industries.This study quantified GHG emissions from aquaculture and mariculture by four source phases(feed,energy use,nitrous oxide and fertilizers),and then analyzed the carbon footprint of each of these phases for GHG production of nine major species groups over the past ten years to show the spatial distribution of GHG emissions from aquaculture and mariculture in China.Our results showed that the production of feed materials contributed most to the GHG emissions and found that crop energy use,crop land use changes(LUC),fertilizer production,crop nitrous oxide production and rice methane production were the main sources of feed emissions.The total GHG emissions of the nine species groups were 112 Mt(10^(9) kg)CO_(2)e,the nine species accounting for approximately 86%of aquaculture and mariculture production.GHG emissions of cyprinids had the highest contribution at 47%.Spatial analysis based on our study showed Guangdong,Hubei,Jiangsu and Shandong had the highest GHG emissions of all the provinces in this study,and they accounted for approximately 46%of all emissions.The regional Gross Domestic Product(GDP)was significantly positively correlated with GHG emissions in every province,with a correlation coefficient higher than 0.6.Our results showed for the first time the relationship between the relative production by species composition and spatial distribution of GHG emissions from aquaculture and mariculture in China.Our findings provide the scientific basis for reduction of GHG emissions within a broader context of expanding aquaculture in the future. 展开更多
关键词 Greenhouse gas emissions AQUACULTURE MARICULTURE Species group FEED Carbon footprint Regional gross domestic product
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Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China 被引量:2
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作者 MA Yuexin TAO Wei +4 位作者 LIU Changfa LIU Jiao YANG Zhiping LI Jin LIU Jichen 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2017年第6期80-88,共9页
The effects of nitrogen (N) addition on microbial biomass, bacterial abundance, and community composition in sediment colonized by Suaeda heteroptera were examined by chloroform fumigation extraction method, real-ti... The effects of nitrogen (N) addition on microbial biomass, bacterial abundance, and community composition in sediment colonized by Suaeda heteroptera were examined by chloroform fumigation extraction method, real-time quantitative polymerase chain reaction, and denaturing gradient gel electrophoresis (DGGE) in a salt marsh located in Shuangtai Estuary, China. The sediment samples were collected from plots treated with different amounts of a single N fertilizer (urea supplied at 0.1, 0.2, 0.4 and 0.8 g/kg (nitrogen content in sediment) and different forms of N fertilizers (urea, (NH4)2SO4, and NH4NO3, each supplied at 0.2 g/kg (calculated by nitrogen). The fertilizers were applied 1-4 times during the plant-growing season in May, luly, August, and September of 2013. Untreated plots were included as a control. The results showed that both the amount and form of N positively influenced microbial biomass carbon, microbial biomass nitrogen, and bacterial abundance. The DGGE profiles revealed that the bacterial community composition was also affected by the amount and form of N. Thus, our findings indicate that short-term N amendment increases microbial biomass and bacterial abundance, and alters the structure of bacterial community. 展开更多
关键词 FERTILIZATION microbial biomass 16S rRNA gene abundance bacterial community salt marsh
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Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region 被引量:1
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作者 ZHANG Xichang LIANG Yubo +5 位作者 FAN Jingfeng ZHANG We PU Hongyu LIANG Bin CHEN Hongxing SONG Lichao 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2005年第5期139-144,共6页
Genomic DNA was extracted from hypnospores of Perkinsus-like parasite of Manila clam Ruditapes philippinarum collected at the fishing grounds in Huanghai Sea coast Shicheng Island and East China Sea coast Ningbo, Chin... Genomic DNA was extracted from hypnospores of Perkinsus-like parasite of Manila clam Ruditapes philippinarum collected at the fishing grounds in Huanghai Sea coast Shicheng Island and East China Sea coast Ningbo, China. The internal transcribed spacer(ITS) in rDNA was PCR-amplified, cloned, sequenced, and compared with that of five Perkinsus species in GenBank. The fragment amplified from DNA of parasite of either Shicheng Island or Ningbo contained 649 bp, including partial ssrRNA(51 bp) and ITS(+5.8 S) (598 bp) regions. The ITS(+5.SS) sequences of Perkinsus-like parasite of both Shicheng Island and Ningbo were all 99% identical to those ofPerkinsis atlanticus, and were not more than 95% identical to those of other four Perkinsus species including P. marinus, P. andrewsi, P. qugwadi and P. medierraneus.The ITS (+5.8S) sequence of Perkinsus-like parasite of Shicheng Island was 99% identical to that of Ningbo. These facts about nucleotide sequences suggested that the Perkinsus-like parasite in Manila clam, Ruditapes philippinarum collected from either the Huanghai Sea coast or the East China Sea coast was P. atlanticus, and might reflect P. atlanticus strains of distinct geographic distribution. 展开更多
关键词 PERKINSUS PCR ITS Manila clam
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Biological Fingerprinting Analysis of Interaction Between Taxoids in Taxus and Microtubule Protein by Microdialysis Coupled with High-performance Liquid Chromatography/Mass Spectrometry for Screening Antimicrotubule Agents 被引量:1
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作者 LEI Xiao-yuan KONG Liang +2 位作者 SU Xing-ye GUO Ming ZOU Han-fa 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2008年第4期411-419,共9页
Some natural products, such as traditional Chinese medicines(TCMs), contain compounds with anticancer activity and have attracted a great interest in recent years as alternative anticancer therapies. A quick and con... Some natural products, such as traditional Chinese medicines(TCMs), contain compounds with anticancer activity and have attracted a great interest in recent years as alternative anticancer therapies. A quick and convenient assay for screening antimicrotubule compounds in which in vitro microdialysis/high-performance liquid chromatography (HPLC) is used to monitor the binding of the compounds extracted from TCM Taxus cuspidata Siebold & Zucc(Taxus) to microtubules is reported. It was observed that the extract of Taxus contains at least five compounds which have affinity interaction with microtubules by biological fingerprinting analysis, and they were identified as the taxoids of taxol, baccatin III, 10-deacetylbaccatin Ⅲ(10-DAB), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT) based on the comparison of their high-performance liquid chromatographic/mass spectrometric and UV spectra with those of the standard samples, both assembly-promoting and disassembly-inhibiting characteristics of those compounds were evaluated. It was observed that baccatin Ⅲ and 10-DAB bound to microtubules and the binding degrees were influenced by GTP. Competitive binding behavior of taxol with other four taxoids to microtubules was also investigated. 展开更多
关键词 Microdialysis/high-performance liquid chromatography(HPLC) Biological fingerprinting analysis TAXOIDS MICROTUBULE TAXUS
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Reproductive biology of Schizopygopsis younghusbandi Regan 1905(Cyprinidae:Schizothoracinae)in the middle reaches of Yarlung Tsangpo River,China 被引量:1
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作者 DUAN Youjian HUO Bin +2 位作者 MA Baoshan YANG Xin XIE Congxin 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2018年第5期1825-1834,共10页
The reproductive biology of Schizopygopsis younghusbandi(Cyprinidae: Schizothoracinae) was studied by 719 individuals that collected from August 2008 to August 2009 in the middle reaches of Yarlung Tsangpo River, Chin... The reproductive biology of Schizopygopsis younghusbandi(Cyprinidae: Schizothoracinae) was studied by 719 individuals that collected from August 2008 to August 2009 in the middle reaches of Yarlung Tsangpo River, China. We coupled monthly variations of the gonadosomatic index(GSI), monthly proportions of the macroscopic maturity stages with size distribution of oocytes to evaluate spawning seasons. Taken as a set, these results indicated that S. younghusband i spawned from March to April with high degree of the spawning synchronicity. The standard lengths and the ages at 50% maturity for male were 222 mm and 4.4 year, and 308 mm and 7.0 year for female. In addition, the estimated mean fecundity and mean relative fecundity were 18682 and 57.8 eggs per g body weight. The fecundity of S. younghusbandi increased linearly with increasing of standard length and body weight. This study provides details about the S. younghusbandi reproduction suggesting that may be this species is vulnerable to exploitation in the middle reaches of Yarlung Tsangpo River. 展开更多
关键词 REPRODUCTION FECUNDITY spawning season Tibet
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Response of ammonia-oxidizing betaproteobacteria to short- term fertilization in a salt marsh in China 被引量:2
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作者 MA Yuexin TAO Wei +3 位作者 LIU Jiao LIU Changfa LI Jin LIU Jichen 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2018年第2期351-361,共11页
This study examines the impacts of short-term(6 months) fertilization on the community structure and abundance of ammonia-oxidizing betaproteobacteria(β-AOB) and the potential nitrification rate in sediment colonized... This study examines the impacts of short-term(6 months) fertilization on the community structure and abundance of ammonia-oxidizing betaproteobacteria(β-AOB) and the potential nitrification rate in sediment colonized by S uaeda heteroptera in a saltmarsh located in Shuangtai estuary, China. The sediment samples were collected from plots treated with different amounts of an N fertilizer(urea supplied at 0.1, 0.2, 0.4, and 0.8 g/kg(nitrogen content in dry sediment)), and with different forms of N fertilizers(urea,(NH4) 2 SO 4, and NH_4NO_3, each supplied at 0.2 g/kg). The fertilizers were applied 1–4 times during the plant-growing season in May, July, August and September of 2013. Untreated plots were included as a control. As revealed in denaturing gradient gel electrophoresis of the 16 S r RNA gene, the β-AOB community responded to both the amount and form of N. Real-time quantitative PCR indicated that both abundance and potential nitrification rate of β-AOB increased after N addition, regardless of concentration and form(except NH_4NO_3). These results provide evidence that short-term N application influences the sediment β-AOB community, β-AOB abundance and potential nitrification rate in a saltmarsh ecosystem. 展开更多
关键词 ammonia-oxidizing betaproteobacteria (β-AOB) denaturing gradient gel electrophoresis (DGGE) nitrification FERTILIZATION SALTMARSH
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A psychophysical-based fuzzy image interpolation algorithm
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作者 余庆军 余报楚 《Journal of Chongqing University》 CAS 2009年第2期145-150,共6页
To produce a smoother and more natural interpolated image, and to preserve and enhance original image details, we defined three perception-based local statistic parameters, namely contrast, noise visibility, and edge ... To produce a smoother and more natural interpolated image, and to preserve and enhance original image details, we defined three perception-based local statistic parameters, namely contrast, noise visibility, and edge strength based on three psychophysical principles, including Weber’s Law, Fechner’s Law, and Stevens’ Power Law, and integrated these parameters into a fuzzy logic system to set up an advanced image interpolation algorithm. Application of this algorithm to detect edge behaviors and local statistical information of images demonstrated better noise removal ability and obtained sharper edges than traditional image interpolation algorithems such as nearest neighbor, bilinear and bicubic interpolation methods. 展开更多
关键词 image interpolation PSYCHOPHYSICS noise visibility and fuzzy logic
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