Artemisia argyi Le´vl.et Vant.,a perennial Artemisia herb with an intense fragrance,is widely used in traditional medicine in China and many other Asian countries.Here,we present a chromosome-scale genome assembl...Artemisia argyi Le´vl.et Vant.,a perennial Artemisia herb with an intense fragrance,is widely used in traditional medicine in China and many other Asian countries.Here,we present a chromosome-scale genome assembly of A.argyi comprising 3.89 Gb assembled into 17 pseudochromosomes.Phylogenetic and comparative genomic analyses revealed that A.argyi underwent a recent lineage-specificwhole-genomeduplication(WGD)event after divergence fromArtemisia annua,resulting in two subgenomes.Wedeciphered the diploid ancestral genome of A.argyi,and unbiased subgenome evolution was observed.The recent WGD led to a large number of duplicated genes in the A.argyi genome.Expansion of the terpene synthase(TPS)gene family through various types of gene duplication may have greatly contributed to the diversity of volatile terpenoids in A.argyi.In particular,we identified a typical germacrene D synthase gene cluster within the expanded TPS gene family.The entire biosynthetic pathways of germacrenes,(+)-borneol,and(+)-camphor were elucidated in A.argyi.In addition,partial deletion of the amorpha-4,11-diene synthase(ADS)gene and loss of function of ADS homologs may have resulted in the lack of artemisinin production in A.argyi.Our study provides newinsights into the genome evolution of Artemisia and lays a foundation for further improvement of the quality of this important medicinal plant.展开更多
A field experiment was conducted at Hudeiba Research Station Farm, located at Ed-Damer, Sudan during 2011/2012 and 2012/2013 winter seasons to investigate the effect of different irrigation regimes and varieties on ch...A field experiment was conducted at Hudeiba Research Station Farm, located at Ed-Damer, Sudan during 2011/2012 and 2012/2013 winter seasons to investigate the effect of different irrigation regimes and varieties on chickpea (Cicer arietinum L.) yield, yield components and water productivity. The treatments include three irrigation regimes;irrigation every 10 days (I1 = full irrigation), irrigation every 15 days (I2 = moderate stress) and irrigation every 20 days (I3 = severe stress) and two varieties (Borgieg and Wad Hamid). The treatments were arranged in factorial randomized complete block design (RCBD) with 3 replications. Irrigation water being applied, grain yield, yield components (number of pods per plant, number of seeds per pod and the 100 seeds weight) and crop water productivity (CWP) and irrigation water productivity (IWP) were recorded. Results showed that the number of pods per plant, number of seeds per pod, 100-seeds weight, grain yield and irrigation water applied were significantly (p ≤ 0.001) affected by irrigation regimes. The highest values of these traits obtained with full irrigation, whereas the lowest values were recorded under severe water stress conditions. Results also indicated that, moderate and severe water stress regimes saved irrigation water by 24% and 32%, respectively compared with full irrigation. This study indicated that treatment I1 which was irrigated every 10-days did not produce the highest IWP, while treatment I2 which irrigated every 15-days gave the highest IWP. The lowest IWP occurred at severe water stress regime (I3). It could be concluded that moderate water stress might be adopted. Contrarily, the adoption of severe water stressed that produce high water savings would lead to yield losses that might be economically not acceptable. The late maturing chickpea variety of Borgieg significantly (p ≤ 0.05) out-yielded the early maturing variety Wad Hamid by 11%. Borgieg displayed the highest values of CWP and IWP.展开更多
基金supported by the National Natural Science Foundation of China(81973422 and 31570302)the Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(2021-I2M-1-071).
文摘Artemisia argyi Le´vl.et Vant.,a perennial Artemisia herb with an intense fragrance,is widely used in traditional medicine in China and many other Asian countries.Here,we present a chromosome-scale genome assembly of A.argyi comprising 3.89 Gb assembled into 17 pseudochromosomes.Phylogenetic and comparative genomic analyses revealed that A.argyi underwent a recent lineage-specificwhole-genomeduplication(WGD)event after divergence fromArtemisia annua,resulting in two subgenomes.Wedeciphered the diploid ancestral genome of A.argyi,and unbiased subgenome evolution was observed.The recent WGD led to a large number of duplicated genes in the A.argyi genome.Expansion of the terpene synthase(TPS)gene family through various types of gene duplication may have greatly contributed to the diversity of volatile terpenoids in A.argyi.In particular,we identified a typical germacrene D synthase gene cluster within the expanded TPS gene family.The entire biosynthetic pathways of germacrenes,(+)-borneol,and(+)-camphor were elucidated in A.argyi.In addition,partial deletion of the amorpha-4,11-diene synthase(ADS)gene and loss of function of ADS homologs may have resulted in the lack of artemisinin production in A.argyi.Our study provides newinsights into the genome evolution of Artemisia and lays a foundation for further improvement of the quality of this important medicinal plant.
文摘A field experiment was conducted at Hudeiba Research Station Farm, located at Ed-Damer, Sudan during 2011/2012 and 2012/2013 winter seasons to investigate the effect of different irrigation regimes and varieties on chickpea (Cicer arietinum L.) yield, yield components and water productivity. The treatments include three irrigation regimes;irrigation every 10 days (I1 = full irrigation), irrigation every 15 days (I2 = moderate stress) and irrigation every 20 days (I3 = severe stress) and two varieties (Borgieg and Wad Hamid). The treatments were arranged in factorial randomized complete block design (RCBD) with 3 replications. Irrigation water being applied, grain yield, yield components (number of pods per plant, number of seeds per pod and the 100 seeds weight) and crop water productivity (CWP) and irrigation water productivity (IWP) were recorded. Results showed that the number of pods per plant, number of seeds per pod, 100-seeds weight, grain yield and irrigation water applied were significantly (p ≤ 0.001) affected by irrigation regimes. The highest values of these traits obtained with full irrigation, whereas the lowest values were recorded under severe water stress conditions. Results also indicated that, moderate and severe water stress regimes saved irrigation water by 24% and 32%, respectively compared with full irrigation. This study indicated that treatment I1 which was irrigated every 10-days did not produce the highest IWP, while treatment I2 which irrigated every 15-days gave the highest IWP. The lowest IWP occurred at severe water stress regime (I3). It could be concluded that moderate water stress might be adopted. Contrarily, the adoption of severe water stressed that produce high water savings would lead to yield losses that might be economically not acceptable. The late maturing chickpea variety of Borgieg significantly (p ≤ 0.05) out-yielded the early maturing variety Wad Hamid by 11%. Borgieg displayed the highest values of CWP and IWP.
