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水杉孑遗居群AFLP遗传变异的空间分布 被引量:21
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作者 李作洲 龚俊杰 +1 位作者 王瑛 黄宏文 《生物多样性》 CAS CSCD 2003年第4期265-275,共11页
本研究采用空间自相关分析方法对水杉 (Metasequoiaglyptostroboides)孑遗居群AFLP遗传变异的空间结构进行了研究 ,以探讨水杉孑遗居群遗传变异的分布特征及其形成机制。根据 6对AFLP选择性引物扩增的 46个多态性位点 ,选择了其表型频率... 本研究采用空间自相关分析方法对水杉 (Metasequoiaglyptostroboides)孑遗居群AFLP遗传变异的空间结构进行了研究 ,以探讨水杉孑遗居群遗传变异的分布特征及其形成机制。根据 6对AFLP选择性引物扩增的 46个多态性位点 ,选择了其表型频率在 2 5 %~ 75 %的 2 7个AFLP标记 ,运用等样本频率方法和等地理距离间隔方法分别对 3 9株和 3 7株原生母树进行了空间自相关系数Moran’sI值计算。结果表明 :水杉孑遗居群缺乏空间结构 ,绝大多数AFLP位点变异为随机分布的空间模式 ,但也有少数位点存在显著性随机相关 ,在 4~ 8km地理距离间隔显示负相关 ,说明该间隔可能是水杉孑遗居群的部分基因交流的有效屏障。水杉原生母树分布存在 12~ 2 8km的明显距离间隔空挡 ,说明人类从迁入该区域起就影响着水杉孑遗居群的原始生境 ,导致其生境片断化、景观破碎 ,进而形成岛屿状分布格局 ,并引起了水杉残留居群的随机遗传漂变。根据本研究结果 ,结合水杉孑遗居群较低的遗传多样性 ,分析探讨了水杉孑遗居群濒危的机理 ,并提出了相应的保育策略 。 展开更多
关键词 空间自相关 保护遗传学 孑遗植物 遗传结构 水杉 遗传变异 空间分布
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一株多种耐药结核菌在纽约全市的暴发流行 被引量:1
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作者 A.R.Moss D.Alland +17 位作者 E.Telzak D.HewlettJr V.Sharp P.Chiliade V.LaBombardi D.Kabus B.Hanna L.Palumbo K.Brudney A.Weltman K.Stoeckle K.Chirgwin M.Simberkoff S.Moghazeh W.Eisner M.Lutfey B.Kreiswirth 丁北川 《国际结核病与肺部疾病杂志》 1997年第2期46-52,共7页
背景:1992年纽约市医院耐两种或更多种药物的活动性结核病情况。目的:检查纽约市1992年结核发病高峰期间,经鉴定命名为公共卫生研究所(PHRI)W株的具有高度耐药且有17条IS6110印迹带的结核菌在全市的分布情况。同时还将此W株与其它纽约... 背景:1992年纽约市医院耐两种或更多种药物的活动性结核病情况。目的:检查纽约市1992年结核发病高峰期间,经鉴定命名为公共卫生研究所(PHRI)W株的具有高度耐药且有17条IS6110印迹带的结核菌在全市的分布情况。同时还将此W株与其它纽约市常见菌株加以比较。设计:应用双盲回归法对保存的结核菌培养物进行限定性片段长度多态性(RFLP)DNA指纹分析。结果:1992年中,我们在21所医院的住院病人中共分离112株W印迹株和8个变株。几乎所有的分离株均对四种一线药物和卡那霉素(KAN)耐药。1992年此单一菌株至少占纽约市多种耐药(MDR)结核病的22%,远高于其它任何一株。几乎所有的W株病例均是AIDS患者。该菌群为纽约市耐药程度最高且迄今为止与其它地区比较所鉴定出的最大的具有IS6110指纹相似性的菌群。结论:由于推荐的四药化疗方案对此高度耐药的菌群不能有效杀灭,因此90年代初在纽约AIDS患者中发生因W株感染而引起的结核暴发流行。其它常见菌株并非呈现高度耐药,甚至于令人惊奇的是表现为全敏感。单一多种耐药菌株能够在AIDS和结核均常见的地区引起广泛的播散。 展开更多
关键词 结核 耐药 多种耐药(MDR) 暴发 纽约
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Incipient sexual isolation in Laupala cerasina: Females discriminate population-level divergence in acoustic characters 被引量:1
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作者 Jaime L. GRACE Kerry L. SHAW 《Current Zoology》 SCIE CAS CSCD 2012年第3期416-425,共10页
Sexual selection by female choice can shape the evolution of male traits within populations, since the most attractive males experience an increase in fitness through elevated mating success. Speciation by sexual sele... Sexual selection by female choice can shape the evolution of male traits within populations, since the most attractive males experience an increase in fitness through elevated mating success. Speciation by sexual selection occurs when evolution in traits and preferences within populations causes differentiation among populations, such that females in alternative populations prefer sexual signals of their own population relative to others. Differentiated traits and preferences thereby play an active role in limiting gene flow between divergent populations. The effectiveness of differentiated preferences in maintaining differentiated male signals against the homogenizing effects of gene flow across populations will be limited by both the degree to which fe- males can discriminate against non-local males, and the breeding values of traits and preferences. Populations of the Hawaiian cricket Laupala cerasina have diverged in pulse rate, a sexually selected male signal, and female acoustic preference for pulse rate. Gene flow between neighboring populations may be reduced if migrants from sexually diverged populations experience re- duced mating success. We show that females discriminate among divergent songs characteristic of neighboring populations, that differences among populations in song and preference breed true in a common environment, and that mean preferences for each population closely match the mean pulse rates. Divergence in preference was observed only between populations that also dif- fered in song. Along with a striking ability to discriminate slight differences in song, correlated evolution of song and preference within populations could be a mechanism that promotes assortative mating among populations, thereby reducing gene flow, and leading to speciation in Laupala [Current Zoology 58 (3): 416-425, 2012]. 展开更多
关键词 Sexual selection Phenotypic divergence Behavioral isolation Preference functions SPECIATION
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Spectral Target-Detecting System Using Sine-Wave Modulation 被引量:1
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作者 DENG Wei ZHAO Chun-jiang +2 位作者 ZHANG Lu-da CHENG Li-ping Andrew Landers 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2011年第10期2771-2777,共7页
Target detection is one of the key technology of precision chemical application.Previously the digital coding modulation technique was commonly used to emit and receive the optical signal in the target detection syste... Target detection is one of the key technology of precision chemical application.Previously the digital coding modulation technique was commonly used to emit and receive the optical signal in the target detection systems previously in China.It was difficult to adjust the output power,and the anti-interference ability was weak in these systems.In order to resolve these problems,the target detection method based on analog sine-wave modulation was studied.The spectral detecting system was set up in the aspects of working principle,electric circuit,and optical path.Lab testing was performed.The results showed that the reflected signal from the target varied inversely with detection distances.It indicated that it was feasible to establish the target detection system using analog sine-wave modulation technology.Furthermore,quantitative measurement of the reflected optical signal for near-infrared and visible light could be achieved by using this system.The research laid the foundation for the future development of the corresponding instrument. 展开更多
关键词 Sine-wave modulation ANALOG Target detection Optical spectrum
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Pentacene-based nanorods on Au(111) single crystals: Charge transfer, diffusion, and step-edge barriers
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作者 Sabine-Antonia Savu Sabine Abb +7 位作者 Simon Schundelmeier Jonathan D. Saathoff James M. Stevenson Christina Tonshoff Holger F. Bettinger Paulette Clancy M. Benedetta Casu Thomas Chasse 《Nano Research》 SCIE EI CAS CSCD 2013年第6期449-459,共11页
We investigate nanorod assemblies of two 64-substituted pentacenes, namely (2,3-X2-9,10-Y2)-substituted pentacenes with X -- Y = OCH3 (MOP) and with X = F, Y-- OCH3 (MOPF), grown on Au(111) single crystals. By... We investigate nanorod assemblies of two 64-substituted pentacenes, namely (2,3-X2-9,10-Y2)-substituted pentacenes with X -- Y = OCH3 (MOP) and with X = F, Y-- OCH3 (MOPF), grown on Au(111) single crystals. By using a multi-technique approach based on ultraviolet photoelectron spectroscopy X-ray photoelectron spectroscopy; and X-ray absorption, we find evidence for charge transfer screening at the interface with gold. Furthermore, the MOP and MOPF nanorods show a rough surface morphology, which was investigated with atomic force microscopy. We use molecular simulation techniques to investigate the energetic barriers to diffusion and to traverse step-edges to estimate their influence on the nanorod roughness. We find that barriers to surface diffusion on a terrace are anisotropic and that their direction favors the formation of nanorods in these materials. 展开更多
关键词 nanorod assembly substituted pentacene electronic structures/ processes/mechanisms organic electronics charge transfer diffusion and step-edge barrier
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Spectroscopic Evaluation of Effects of Heat Treatments on the Structures and Emulsifying Properties of Caseins
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作者 ZHANG Hao WANG Peng-jie +4 位作者 LEI Xin-gen YANG Hong-ju ZHANG Lu-da REN Fa-zheng ZHENG Li-min 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2013年第5期1275-1280,共6页
The effects of heat treatment(heating temperature and pH) on the structures and emulsifying properties of caseins were systematically studied by spectroscopy.Heat treatment from 60to 100℃resulted in an increase in th... The effects of heat treatment(heating temperature and pH) on the structures and emulsifying properties of caseins were systematically studied by spectroscopy.Heat treatment from 60to 100℃resulted in an increase in their fluorescence intensity,hydrodynamic diameter,turbidity and emulsifying activity index,but decreased the size polydispersity of caseins.In the pH range of 5.5to 7.0,the fluorescence intensity,hydrodynamic diameter,turbidity and emulsifying properties decreased with increased heating pH,but the size polydispersity of caseins increased with increased pH.The relationship between the surface fluorescence intensity and emulsifying activity was also investigated,revealing a correlation coefficient of 0.90.These results suggested that heat treatment could be used to modify the structures and emulsifying properties of caseins by appropriately selecting heating conditions. 展开更多
关键词 CASEINS Heat treatment STRUCTURES Emulsifying properties Fluorescence spectrometry Dynamic light scattering TURBIDITY
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