In Arabidopsis thaliana,canonical auxin-dependent gene regulation is mediated by 23 transcription factors from the AUXIN RESPONSE FACTOR(ARF)family that interact with auxin/indole acetic acid repressors(Aux/IAAs),whic...In Arabidopsis thaliana,canonical auxin-dependent gene regulation is mediated by 23 transcription factors from the AUXIN RESPONSE FACTOR(ARF)family that interact with auxin/indole acetic acid repressors(Aux/IAAs),which themselves form co-receptor complexes with one of six TRANSPORT INHIBITOR*!/AUXIN-SIGNALLING F-BOX(TIR1/AFB)proteins.Different combinations of co-receptors drive specific sensing outputs,allowing auxin to control a myriad of processes.ARF6 and ARF8 are positive regulators of adventitious root initiation upstream of jasmonate,but the exact auxin co-receptor complexes controlling the transcriptional activity of these proteins has remained unknown.Here,using loss-of-function mutants we show that three Aux/IAA genes,IAA6,IAA9,and IAA17,act additively in the control of adventitious root(AR)initiation.These three IAA proteins interact with ARF6 and/or ARF8 and likely repress their activity in AR development.We show that TIR1 and AFB2 are positive regulators of AR formation and TIR1 plays a dual role in the control of jasmonic acid(JA)biosynthesis and conjugation,as several JA biosynthesis genes are up-regulated in the tir1-1 mutant.These results lead us to propose that in the presence of auxin,TIR1 and AFB2 form specific sensing complexes with IAA6,IAA9,and/or IAA17 to modulate JA homeostasis and control AR initiation.展开更多
Human-wildlife conflict(HWC)is a conservation concern that increasingly threatens the continued existence of some of the world’s most endangered species.With an increase in human population,urban sprawl and sub...Human-wildlife conflict(HWC)is a conservation concern that increasingly threatens the continued existence of some of the world’s most endangered species.With an increase in human population,urban sprawl and subsequent encroachment on wild land,human and wildlife interaction has become inevitable.In the majority of cases,this interaction results in a negative outcome for humans,wildlife or both.In China,these key elements,along with a decrease in wild prey species,have resulted in the expansion of HWC encounters,and the need for alleviating this conflict has become a conservation priority.Loss of human life,livestock and/or crops is most often the catalysts that fuel HWC.Techniques to alleviate conflict around the world have included preventative measures and mitigation techniques,such as financial compensation and other incentive programs.Both types of measures have had variable success.We review the current status of human-carnivore conflict management in China,and,drawing lessons from around the globe,we make recommendations for improving conservation management in China.For example,an increase in law enforcement in nature reserves is vital to reducing human disturbance in prime carnivore habitat,thereby reducing conflict encounters.Also,modifications to current wildlife compensation programs,so that they are linked with preventative measures,will ensure that moral hazards are avoided.Furthermore,investigating the potential for a community self-financed insurance scheme to fund compensation and increasing efforts to restore wild prey populations will improve the outcome for wildlife conservation.Ultimately,HWC management in China will greatly benefit from an integrative approach.展开更多
Background: The interaction of the gut microbiota with key metabolic and physiological processes may be associated with poor growth outcomes in animals born with intrauterine growth restriction(IUGR).Results: Growth p...Background: The interaction of the gut microbiota with key metabolic and physiological processes may be associated with poor growth outcomes in animals born with intrauterine growth restriction(IUGR).Results: Growth performance, plasma hormone concentrations, and intestinal microbiota composition were analyzed in IUGR pigs and in normal birth weight(NBW) pigs when the NBW pigs reached 25, 50, and 100 kg of body weight(BW). Compared to NBW pigs, IUGR pigs had lower initial, weaned, and final BW, and lower average daily gain and average daily feed intake in all the considered time points. In the 25 kg BW group, IUGR pigs had higher concentrations of plasma ghrelin and pancreatic polypeptide(PP), but lower insulin concentration than NBW pigs, while the situation was reversed in the 50 kg BW group. As compared to NBW pigs, IUGR pigs had higher microbial alpha diversity in the jejunum and ileum;in the 50 and 100 kg BW groups, IUGR pigs had higher Firmicutes abundance but lower Proteobacteria abundance in the jejunum, and lower Lactobacillus abundance in the jejunum and ileum;in the 25 kg BW group, IUGR pigs showed higher unclassified Ruminococcaceae abundance in the ileum;and in 25 and 50 kg BW groups, IUGR pigs showed lower Ochrobactrum abundance in the jejunum.Spearman's correlation revealed that Lactobacillus was negatively correlated with growth performance, while unclassified Ruminococcaceae was positively correlated. Predictive metagenomic analysis detected significantly different expression of genes in the intestinal microbiota between IUGR and NBW pigs, suggesting different metabolic capabilities between the two groups.Conclusions: Growing-finishing IUGR pigs showed lower growth performance, higher microbial alpha diversity, and differences in plasma hormone concentrations compared to NBW pigs. Alterations in the abundance of Firmicutes,Proteobacteria, Ruminococcaceae, Lactobacillus, and Ochrobactrum in the small intestine may be associated with IUGR, and may therefore serve as a future target for gut microbio展开更多
Several components of predator functional diversity have been hypothesized to influence prey suppression through either niche complementarity or mass ratio effects.Nevertheless,most studies have used a functional grou...Several components of predator functional diversity have been hypothesized to influence prey suppression through either niche complementarity or mass ratio effects.Nevertheless,most studies have used a functional group approach when assessing the role of these predators in ecosystem functioning.By adopting a trait-based approach,we evaluated the relative contributions of carabid diversity components in predicting prey suppression.Our results highlight the importance of both taxonomic and functional diversity components of carabids as key drivers of prey suppression.Prey suppression was best predicted by carabid densities,with the dominance of Poecilus cupreus potentially driving the positive effect of community total abundance through the mass ratio effect.Prey suppression increased with increasing the density of large carabids.In addition,carabid eye diameter and antennal length were key functional traits for predicting prey suppression.Furthermore,prey suppression increased with increasing carabid functional richness following the niche complementarity effect.In contrast to functional richness,functional evenness and functional divergence of carabid communities were weakly correlated with prey suppression.By identifying which diversity components of carabid communities contribute the most to increase prey suppression,our results can guide efforts aiming to predict the relationship between diversity of these predators and ecosystem functioning.展开更多
BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases. To achieve this objective, overlapping actions of complex cellular processes, such as ...BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases. To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required. These events are longitudinally and tightly controlled by numerous factors including a wide range of distinct regulatory proteins. However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.AIM To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced colitis.METHODS C57 BL/6 male mice were given 3.5% dextran sodium sulfate(DSS) in drinking water for 5 d. They were euthanized 2(day 7), 5(day 10), 8(day 13), and 23(day28) d after DSS removal. The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0. Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16 S rRNA sequencing.RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa. It was recorded 2 d after termination of the DSStreatment, followed by a progressive return to values similar to those of control mice. Although signs of colitis were severe(inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations(hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of cert展开更多
Bayesian inference model is an optimal processing of incomplete information that, more than other models, better captures the way in which any decision-maker learns and updates his degree of rational beliefs about pos...Bayesian inference model is an optimal processing of incomplete information that, more than other models, better captures the way in which any decision-maker learns and updates his degree of rational beliefs about possible states of nature, in order to make a better judgment while taking new evidence into account. Such a scientific model proposed for the general theory of decision-making, like all others in general, whether in statistics, economics, operations research, A.I., data science or applied mathematics, regardless of whether they are time-dependent, have in common a theoretical basis that is axiomatized by relying on related concepts of a universe of possibles, especially the so-called universe (or the world), the state of nature (or the state of the world), when formulated explicitly. The issue of where to stand as an observer or a decision-maker to reframe such a universe of possibles together with a partition structure of knowledge (i.e. semantic formalisms), including a copy of itself as it was initially while generalizing it, is not addressed. Memory being the substratum, whether human or artificial, wherein everything stands, to date, even the theoretical possibility of such an operation of self-inclusion is prohibited by pure mathematics. We make this blind spot come to light through a counter-example (namely Archimedes’ Eureka experiment) and explore novel theoretical foundations, fitting better with a quantum form than with fuzzy modeling, to deal with more than a reference universe of possibles. This could open up a new path of investigation for the general theory of decision-making, as well as for Artificial Intelligence, often considered as the science of the imitation of human abilities, while being also the science of knowledge representation and the science of concept formation and reasoning.展开更多
Since its discovery as a bacterial adaptive immune system and its development for genome editing in eukaryotes,the CRISPR technology has revolutionized plant research and precision crop breeding.The CRISPR toolbox hol...Since its discovery as a bacterial adaptive immune system and its development for genome editing in eukaryotes,the CRISPR technology has revolutionized plant research and precision crop breeding.The CRISPR toolbox holds great promise in the production of crops with genetic disease resistance to increase agriculture resilience and reduce chemical crop protection with a strong impact on the environment and public health.In this review,we provide an extensive overviewon recent breakthroughs in CRISPR technology,including the newly developed prime editing system that allows precision gene editing in plants.We present how each CRISPR tool can be selected for optimal use in accordance with its specific strengths and limitations,and illustrate how the CRISPR toolbox can foster the development of genetically pathogen-resistant crops for sustainable agriculture.展开更多
Modified BCR sequential extraction, single equilibrium-based EDTA extraction and kinetic fractionation were used for estimating the Pb and Cd availability in a series of soil samples from 3 sites located in urban area...Modified BCR sequential extraction, single equilibrium-based EDTA extraction and kinetic fractionation were used for estimating the Pb and Cd availability in a series of soil samples from 3 sites located in urban areas of Hanoi (Vietnam). These schemes were compared to identify a simple, rapid and cheap protocol for routine estimation of Pb and Cd remobilizable fraction and the related potential risk. The comparison of these three approaches revealed their convergence in terms of mobility patterns observed for Pb and Cd. Cd was characterized by higher extractibality and mobility whatever the approach. Pb was distinguished of Cd by its high association to Feoxides, lower extractability, lower rate of desorption and then lower mobility. For this environmental scenario, EDTA single scheme at equilibrium could be suggested as the best suited and a simple protocol for determination of the labile pool of Cd and Pb. The pseudo-total concentrations of Pb and Cd are actually below the Vietnamese standard level, contrary to the results obtained by other authors for agricultural soils at other urban sites of Hanoi.展开更多
Algae have become an area of intensive research in many fields of study. Areas of application are becoming increasingly diverse with the advent of technologies particularly in the mass production of algae biomass. Alg...Algae have become an area of intensive research in many fields of study. Areas of application are becoming increasingly diverse with the advent of technologies particularly in the mass production of algae biomass. Algae contain complex bioactive compounds and these are gaining importance in emerging technologies with nutritional and environmental applications. In this study, a preliminary investigation evaluated 15 species of algae from the major categories of marine and fresh water algae for their potential as inclusions in ruminant diets for management of greenhouse gas emissions. It was hypothesized that algae would positively affect rumen fermentation and gas production while reducing methane production. The hypothesis was tested using an Ankom automated gas monitoring system and rumen fluid from Bos indicus steers fed tropical forage diets. The results were variable between algae species with some showing a significant reduction in total gas and methane production, with others increasing gas and fermentation. The red and brown algae stand out as having potential for greenhouse gas mitigation with the brown alga Cystoseira having the most prominent effect. The effects observed on fermentation may be manipulated through dosage management and beneficial effects could be potentially maximized by preparing combinations of algal supplements. It has been demonstrated in this study that algae have the potential to assist in rumen fermentation management for improved gas production, and greenhouse gas abatement.展开更多
Plant defense responses involve several biological processes that allow plants to fight against pathogenic attacks.How these different processes are orchestrated within organs and depend on specific cell types is poor...Plant defense responses involve several biological processes that allow plants to fight against pathogenic attacks.How these different processes are orchestrated within organs and depend on specific cell types is poorly known.Here,using single-cell RNA sequencing(scRNA-seq)technology on three independent biological replicates,we identified several cell populations representing the core transcriptional responses of wild-type Arabidopsis leaves inoculated with the bacterial pathogen Pseudomonas syringae DC3000.Among these populations,we retrieved major cell types of the leaves(mesophyll,guard,epidermal,companion,and vascular S cells)with which we could associate characteristic transcriptional reprogramming and regulators,thereby specifying different cell-type responses to the pathogen.Further analyses of transcriptional dynamics,on the basis of inference of cell trajectories,indicated that the different cell types,in addition to their characteristic defense responses,can also share similar modules of gene reprogramming,uncovering a ubiquitous antagonism between immune and susceptible processes.Moreover,it appears that the defense responses of vascular S cells,epidermal cells,and mesophyll cells can evolve along two separate paths,one converging toward an identical cell fate,characterized mostly by lignification and detoxification functions.As this divergence does not correspond to the differentiation between immune and susceptible cells,we speculate that this might reflect the discrimination between cellautonomous and non-cell-autonomous responses.Altogether our data provide an upgraded framework to describe,explore,and explain the specialization and the coordination of plant cell responses upon pathogenic challenge.展开更多
Several internet-based surveillance systems have been created to monitor the web for animal health surveillance.These systems collect a large amount of news dealing with outbreaks related to animal diseases.Automatica...Several internet-based surveillance systems have been created to monitor the web for animal health surveillance.These systems collect a large amount of news dealing with outbreaks related to animal diseases.Automatically identifying news articles that describe the same outbreak event is a key step to quickly detect relevant epidemiological information while alleviating manual curation of news content.This paper addresses the task of retrieving news articles that are related in epidemiological terms.We tackle this issue using text mining and feature fusion methods.The main objective of this paper is to identify a textual representation in which two articles that share the same epidemiological content are close.We compared two types of representations(i.e.,features)to represent the documents:(i)morphosyntactic features(i.e.,selection and transformation of all terms from the news,based on classical textual processing steps)and(ii)lexicosemantic features(i.e.,selection,transformation and fusion of epidemiological terms including diseases,hosts,locations and dates).We compared two types of term weighing(i.e.,Boolean and TF-IDF)for both representations.To combine and transform lexicosemantic features,we compared two data fusion techniques(i.e.,early fusion and late fusion)and the effect of features generalisation,while evaluating the relative importance of each type of feature.We conducted our analysis using a corpus composed of a subset of news articles in English related to animal disease outbreaks.Our results showed that the combination of relevant lexicosemantic(epidemiological)features using fusion methods improves classical morphosyntactic representation in the context of disease-related news retrieval.The lexicosemantic representation based on TF-IDF and feature generalisation(F-measure=0.92,r-precision=0.58)outperformed the morphosyntactic representation(F-measure=0.89,r-precision=0.45),while reducing the features space.Converting the features into lower granular features(i.e.,generalisation)contributed to impr展开更多
Sugarcane bagasse was pyrolyzed using a laboratory fixed bed reactor to produce char and its by-product(pyrolysis liquid).The pyrolysis experiments were carried out using different temperatures(400℃ and 500℃),heatin...Sugarcane bagasse was pyrolyzed using a laboratory fixed bed reactor to produce char and its by-product(pyrolysis liquid).The pyrolysis experiments were carried out using different temperatures(400℃ and 500℃),heating rate(1℃/min and 10℃/min),and holding time(30 min and 60 min).Char was characterized according to its thermal properties,while the pyrolysis liquid was tested for its anti-fungal and anti-termite activities.Pyrolysis temperature and heating rate had a significant influence on the char properties and the yield of char and pyrolysis liquid,where a high-quality char and high yield of pyrolysis liquid can be obtained at a temperature of 500℃ and a heating rate of 10℃/min.The yield of char and pyrolysis liquid was 28.97%and 55.46%,respectively.The principal compounds of pyrolysis liquid were water,acetic acid,glycolaldehyde,1-hydroxy-2-propanone,methanol,formic acid,levoglucosan,furfural,followed by some phenol compounds and guaiacol derivatives.Pyrolysis liquid at a concentration of 0.20%and 0.25%(v/v)caused a 100%inhibition of Coniophora puteana and Trametes versicolor,respectively,when performing inhibition growth tests in Petri dishes.Filter paper treated with 10%of pyrolysis liquid caused 100%of termite mortality,while only 5.65%–7.03%of the treated filter papers consumed by termites at such concentration.Pyrolysis liquid is potentially effective to be used in the formulation of wood protection against fungi and termites.展开更多
Quantitative disease resistance(QDR)remains the most prevalent form of plant resistance in crop fields and wild habitats.Genome-wide association studies(GWAS)have proved to be successful in deciphering the quantitativ...Quantitative disease resistance(QDR)remains the most prevalent form of plant resistance in crop fields and wild habitats.Genome-wide association studies(GWAS)have proved to be successful in deciphering the quantitative genetic basis of complex traits such as QDR.To unravel the genetics of QDR to the devastating worldwide bacterial pathogen Ralstonia solanacearum,we performed a GWAS by challenging a highly polymorphic local mapping population of Arabidopsis thaliana with four R.solanacearum type III effector(T3E)mutants,identified as key pathogenicity determinants after a first screen on an A.thaliana core collection of 25 accessions.Although most quantitative trait loci(QTLs)were highly specific to the identity of the T3E mutant(ripAC,ripAG,ripAQ,and ripU),we finely mapped a common QTL located on a cluster of nucleotide-binding domain and leucine-rich repeat(NLR)genes that exhibited structural variation.We functionally validated one of these NLRs as a susceptibility factor in response to R.solanacearum,named it Bacterial Wilt Susceptibility 1(BWS1),and cloned two alleles that conferred contrasting levels of QDR.Further characterization indicated that expression of BWS1 leads to suppression of immunity triggered by different R.solanacearum effectors.In addition,we showed a direct interaction between BWS1 and RipAC T3E,and BWS1 and SUPPRESSOR OF G2 ALLELE OF skp1(SGT1b),the latter interaction being suppressed by RipAC.Together,our results highlight a putative role for BWS1 as a quantitative susceptibility factor directly targeted by the T3E RipAC,mediating negative regulation of the SGT1-dependent immune response.展开更多
The continuation of the isolated Amur tiger(Panthera tigris altaica)population living along the China-Russia border is facing serious challenges due to factors such as its small size(including 38 individuals)and canin...The continuation of the isolated Amur tiger(Panthera tigris altaica)population living along the China-Russia border is facing serious challenges due to factors such as its small size(including 38 individuals)and canine distemper virus(CDV).We use a population viability analysis metamodel,which consists of a traditional individual-based demographic model linked to an epidemiological model,to assess options for controlling the impact of negative factors through domestic dog management in protected areas,increasing connectivity to the neighboring large population(including more than 400 individuals),and habitat expansion.Without intervention,under inbreeding depression of 3.14,6.29,and 12.26 lethal equivalents,our metamodel predicted the extinction within 100 years is 64.4%,90.6%,and 99.8%,respectively.In addition,the simulation results showed that dog management or habitat expansion independently will not ensure tiger population viability for the next 100 years,and connectivity to the neighboring population would only keep the population size from rapidly declining.However,when the above three conservation scenarios are combined,even at the highest level of 12.26 lethal equivalents inbreeding depression,population size will not decline and the probability of extinction will be<5.8%.Our findings highlight that protecting the Amur tiger necessitates a multifaceted synergistic effort.Our key management recommendations for this population underline the importance of reducing CDV threats and expanding tiger occupancy to its former range in China,but re-establishing habitat connectivity to the neighboring population is an important long-term objective.展开更多
Several research studies have proven that eliciting and predicting the impact of human activity on ecosystem services will be crucial to support stakeholders’ awareness and to decide how to interact with the environm...Several research studies have proven that eliciting and predicting the impact of human activity on ecosystem services will be crucial to support stakeholders’ awareness and to decide how to interact with the environment in a more sustainable manner. In this sense, the ecosystems known as road verges are particularly important because of their length and surface at an international scale, and their role in mitigating the damage done by roads. Plant pollination by insects is one of the most important ecosystem services. Because of its nature and the fact that they extend across a variety of landscapes, roadside can contribute to the maintenance of healthy ecosystems, under the condition of adapted management practices. This research is the first attempt to develop a System Dynamics-based aiming to estimate the ecological and economic impact of maintenance on the road verge pollination service in France. Maintenance strategies of road verges are simulated to compare their performance. The results show that there are ways to improve current maintenance strategies in terms of pollination value, but also that the model needs to consider other ecosystem services and synergistic effects that could further affect pollination to obtain more accurate estimations.展开更多
Soil erosion is a complex process involving multiple natural and anthropic agents,causing the deterio-ration of multiple components comprising soil health.Here,we provide an estimate of the spatial pat-terns of cropla...Soil erosion is a complex process involving multiple natural and anthropic agents,causing the deterio-ration of multiple components comprising soil health.Here,we provide an estimate of the spatial pat-terns of cropland susceptibility to erosion by sheet and rill,gully,wind,tillage,and root crops harvesting and report the co-occurrence of these processes using a multi-model approach.In addition,to give a global overview of potential future changes,we identify the locations where these multiple concurrent soil erosion processes may be expected to intersect with projected dry/wet climate changes by 2070.Of a modelled 1.48 billion hectares(B ha)of global cropland,our results indicate that 0.56 B ha(-36%of the total area)are highly susceptible(classes 4 and 5)to a single erosion process,0.27 B ha(-18%of the total area)to two processes and 0.02 B ha(1.4%of the total area)to three or more processes.An estimated 0.82 B ha of croplands are susceptible to possible increases in water(0.68 B ha)and wind(0.14 B ha)erosion.We contend that the presented set of estimates represents a basis for enhancing our founda-tional knowledge on the geography of soil erosion at the global scale.The generated insight on multiple erosion processes can be a useful starting point for decision-makers working with ex-post and ex-ante policy evaluation of the UN Sustainable Development Goal 15(Life on Land)activities.Scientifically,this work provides the hitherto most comprehensive assessment of soil erosion risks at the global scale,based on state-of-the-art models.展开更多
BACKGROUND Chronic abdominal pain is the most common cause for gastroenterology consultation and is frequently associated with functional gastrointestinal disorders including irritable bowel syndrome and inflammatory ...BACKGROUND Chronic abdominal pain is the most common cause for gastroenterology consultation and is frequently associated with functional gastrointestinal disorders including irritable bowel syndrome and inflammatory bowel disease. These disorders present similar brain/gut/microbiota trialogue alterations, associated with abnormal intestinal permeability, intestinal dysbiosis and colonic hypersensitivity(CHS). Intestinal dysbiosis can alter colon homeostasis leading to abnormal activation of the innate immunity that promotes CHS, perhaps involving the toll-like receptors(TLRs), which play a central role in innate immunity.AIM To understand the mechanisms between early life event paradigm on intestinal permeability, fecal microbiota composition and CHS development in mice with TLRs expression in colonocytes.METHODS Maternal separation model(NMS) CHS model, which mimics deleterious events in childhood that can induce a wide range of chronic disorders during adulthood were used. Colonic sensitivity of NMS mice was evaluated by colorectal distension(CRD) coupled with intracolonic pressure variation(IPV) measurement. Fecal microbiota composition was analyzed by 16S rRNA sequencing from weaning to CRD periods. TLR mRNA expression was evaluated in colonocytes.Additionally, the effect of acute intrarectal instillation of the TLR5 agonist flagellin(FliC) on CHS in adult naive wildtype mice was analyzed.RESULTS Around 50% of NMS mice exhibited increased intestinal permeability and CHS associated with intestinal dysbiosis, characterized by a significant decrease of species richness, an alteration of the core fecal microbiota and a specific increased relative abundance of flagellated bacteria. Only TLR5mRNA expression was increased in colonocytes of NMS mice with CHS. Acute intrarectal instillation of FliC induced transient increase of IPV, reflecting transient CHS appearance.CONCLUSION Altogether, these data suggest a pathophysiological continuum between intestinal dysbiosis and CHS, with a role for TLR5.展开更多
Although egg color polymorphism has evolved as an effective defensive adaptation to brood parasitism,spatial variations in egg color polymorphism remain poorly characterized.Here,we investigated egg polymorphism in 64...Although egg color polymorphism has evolved as an effective defensive adaptation to brood parasitism,spatial variations in egg color polymorphism remain poorly characterized.Here,we investigated egg polymorphism in 647 host species(68 families and 231 gen era)parasitized by 41 species of Old Word cuckoos(1 family and 11 gen era)across Asia,Europe,Africa,and Australia.The diversity of parasitic cuckoos differs among continents,reflecting the continent-specific intensities of parasitic selection pressure on hosts.Therefore,host egg polymorphism is expected to evolve more frequently on continents with higher cuckoo diversity.We identified egg polymorphism in 24.1%of all host species and 47.6%of all host families.The common cuckoo Cuculus canorus utilized 184 hosts(28.4%of all host species).Hosts of the common cuckoo and of Chrysococcyx species were more likely to have polymorphic eggs than hosts parasitized by other cuckoos.Both the number of host species and the host families targeted by the cuckoo species were positively correlated with the frequency of host egg polymorphism.Most host species and most hosts exhibiting egg color polymorphism were located in Asia and Africa.Host egg polymorphism was observed less frequently in Australia and Europe.Our results also suggested that egg polymorphism tends to occur more frequently in hosts that are utilized by several cuckoo species or by generalist cuckoo species.We suggest that selecti on pressure on hosts from a given contin ent in creases proportionally to the number of cuckoo species,and that this selection pressure may,in turn,favor the evolution of host egg polymorphism.展开更多
Egg discrimination by cavity-nesting birds that build nests under dim light conditions was presumed to depend on nest luminance,although this hypothesis has rarely been tested.Tests of egg discrimination ability by ca...Egg discrimination by cavity-nesting birds that build nests under dim light conditions was presumed to depend on nest luminance,although this hypothesis has rarely been tested.Tests of egg discrimination ability by cavity-nesting tits under dim light conditions may reveal the selection pressure from brood parasitism that they encounter under natural interactions.We manipulated the intensity of luminance of nests of the Green-backed Tit(Parus monticolus),a potential cuckoo host that possesses a strong discrimination ability of non-mimetic foreign eggs.We performed experiments to test their egg discrimination ability under different light conditions.Our results showed that Green-backed Tits discriminate against non-mimetic foreign eggs under normal light conditions in nest boxes,and this ability persisted at nest luminance as low as 4.78±1.31 lux that is several times lower than normal luminance(38.11±24.02 lux).However,egg discrimination by Green-backed Tits disappeared when nest luminance was reduced to a minimum of 0.35±0.15 lux.The latter value represents total darkness for humans.The present study shows that nest luminance plays a key role in egg discrimination by Green-backed Tits that build nests under dim light conditions.This study provides strong experimental evidence for nest illumination altering egg rejection behavior in cavity-nesting birds.展开更多
Intercropping is the planned cultivation of species mixtures on agricultural land.Intercropping has many attributes that make it attractive for developing a more sustainable agriculture,such as high yield,high resourc...Intercropping is the planned cultivation of species mixtures on agricultural land.Intercropping has many attributes that make it attractive for developing a more sustainable agriculture,such as high yield,high resource use efficiency,lower input requirements,natural suppression of pests,pathogens and weeds,and building a soil with more organic carbon and nitrogen.Information is needed which species combinations perform best under different circumstances and which management is suitable to bring out the best from intercropping in a given production situation.The literature is replete with case studies on intercropping from across the globe,but evidence synthesis is needed to make this information accessible.Meta-analysis requires a careful choice of metric that is appropriate for answering the question at hand,and which lends itself for a robust meta-analysis.This paper reviews some metrics that may be used in the quantitative synthesis of literature data on intercropping.展开更多
文摘In Arabidopsis thaliana,canonical auxin-dependent gene regulation is mediated by 23 transcription factors from the AUXIN RESPONSE FACTOR(ARF)family that interact with auxin/indole acetic acid repressors(Aux/IAAs),which themselves form co-receptor complexes with one of six TRANSPORT INHIBITOR*!/AUXIN-SIGNALLING F-BOX(TIR1/AFB)proteins.Different combinations of co-receptors drive specific sensing outputs,allowing auxin to control a myriad of processes.ARF6 and ARF8 are positive regulators of adventitious root initiation upstream of jasmonate,but the exact auxin co-receptor complexes controlling the transcriptional activity of these proteins has remained unknown.Here,using loss-of-function mutants we show that three Aux/IAA genes,IAA6,IAA9,and IAA17,act additively in the control of adventitious root(AR)initiation.These three IAA proteins interact with ARF6 and/or ARF8 and likely repress their activity in AR development.We show that TIR1 and AFB2 are positive regulators of AR formation and TIR1 plays a dual role in the control of jasmonic acid(JA)biosynthesis and conjugation,as several JA biosynthesis genes are up-regulated in the tir1-1 mutant.These results lead us to propose that in the presence of auxin,TIR1 and AFB2 form specific sensing complexes with IAA6,IAA9,and/or IAA17 to modulate JA homeostasis and control AR initiation.
基金supported by Hunchun National Nature Reserve and the Hunchun Amur Tiger Tianhe Conservation Association.
文摘Human-wildlife conflict(HWC)is a conservation concern that increasingly threatens the continued existence of some of the world’s most endangered species.With an increase in human population,urban sprawl and subsequent encroachment on wild land,human and wildlife interaction has become inevitable.In the majority of cases,this interaction results in a negative outcome for humans,wildlife or both.In China,these key elements,along with a decrease in wild prey species,have resulted in the expansion of HWC encounters,and the need for alleviating this conflict has become a conservation priority.Loss of human life,livestock and/or crops is most often the catalysts that fuel HWC.Techniques to alleviate conflict around the world have included preventative measures and mitigation techniques,such as financial compensation and other incentive programs.Both types of measures have had variable success.We review the current status of human-carnivore conflict management in China,and,drawing lessons from around the globe,we make recommendations for improving conservation management in China.For example,an increase in law enforcement in nature reserves is vital to reducing human disturbance in prime carnivore habitat,thereby reducing conflict encounters.Also,modifications to current wildlife compensation programs,so that they are linked with preventative measures,will ensure that moral hazards are avoided.Furthermore,investigating the potential for a community self-financed insurance scheme to fund compensation and increasing efforts to restore wild prey populations will improve the outcome for wildlife conservation.Ultimately,HWC management in China will greatly benefit from an integrative approach.
基金jointly supported by the National Natural Science Foundation of China (31772613 and 31572421)Special Funds for Construction of Innovative Provinces in Hunan Province (2019RS3022)。
文摘Background: The interaction of the gut microbiota with key metabolic and physiological processes may be associated with poor growth outcomes in animals born with intrauterine growth restriction(IUGR).Results: Growth performance, plasma hormone concentrations, and intestinal microbiota composition were analyzed in IUGR pigs and in normal birth weight(NBW) pigs when the NBW pigs reached 25, 50, and 100 kg of body weight(BW). Compared to NBW pigs, IUGR pigs had lower initial, weaned, and final BW, and lower average daily gain and average daily feed intake in all the considered time points. In the 25 kg BW group, IUGR pigs had higher concentrations of plasma ghrelin and pancreatic polypeptide(PP), but lower insulin concentration than NBW pigs, while the situation was reversed in the 50 kg BW group. As compared to NBW pigs, IUGR pigs had higher microbial alpha diversity in the jejunum and ileum;in the 50 and 100 kg BW groups, IUGR pigs had higher Firmicutes abundance but lower Proteobacteria abundance in the jejunum, and lower Lactobacillus abundance in the jejunum and ileum;in the 25 kg BW group, IUGR pigs showed higher unclassified Ruminococcaceae abundance in the ileum;and in 25 and 50 kg BW groups, IUGR pigs showed lower Ochrobactrum abundance in the jejunum.Spearman's correlation revealed that Lactobacillus was negatively correlated with growth performance, while unclassified Ruminococcaceae was positively correlated. Predictive metagenomic analysis detected significantly different expression of genes in the intestinal microbiota between IUGR and NBW pigs, suggesting different metabolic capabilities between the two groups.Conclusions: Growing-finishing IUGR pigs showed lower growth performance, higher microbial alpha diversity, and differences in plasma hormone concentrations compared to NBW pigs. Alterations in the abundance of Firmicutes,Proteobacteria, Ruminococcaceae, Lactobacillus, and Ochrobactrum in the small intestine may be associated with IUGR, and may therefore serve as a future target for gut microbio
基金supported by an INRAE/Lorraine Region(France)fellowshipthe“CASDAR Entomophages”Project(French Agricultural Ministry).
文摘Several components of predator functional diversity have been hypothesized to influence prey suppression through either niche complementarity or mass ratio effects.Nevertheless,most studies have used a functional group approach when assessing the role of these predators in ecosystem functioning.By adopting a trait-based approach,we evaluated the relative contributions of carabid diversity components in predicting prey suppression.Our results highlight the importance of both taxonomic and functional diversity components of carabids as key drivers of prey suppression.Prey suppression was best predicted by carabid densities,with the dominance of Poecilus cupreus potentially driving the positive effect of community total abundance through the mass ratio effect.Prey suppression increased with increasing the density of large carabids.In addition,carabid eye diameter and antennal length were key functional traits for predicting prey suppression.Furthermore,prey suppression increased with increasing carabid functional richness following the niche complementarity effect.In contrast to functional richness,functional evenness and functional divergence of carabid communities were weakly correlated with prey suppression.By identifying which diversity components of carabid communities contribute the most to increase prey suppression,our results can guide efforts aiming to predict the relationship between diversity of these predators and ecosystem functioning.
基金grants from the Societe Francaise de Nutrition and the Association Francois AupetitVidal-Lletjos S was a recipient of a PhD grant from INRA-Universite Paris-Saclay(ALIAS program)
文摘BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases. To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required. These events are longitudinally and tightly controlled by numerous factors including a wide range of distinct regulatory proteins. However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.AIM To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced colitis.METHODS C57 BL/6 male mice were given 3.5% dextran sodium sulfate(DSS) in drinking water for 5 d. They were euthanized 2(day 7), 5(day 10), 8(day 13), and 23(day28) d after DSS removal. The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0. Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16 S rRNA sequencing.RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa. It was recorded 2 d after termination of the DSStreatment, followed by a progressive return to values similar to those of control mice. Although signs of colitis were severe(inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations(hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of cert
文摘Bayesian inference model is an optimal processing of incomplete information that, more than other models, better captures the way in which any decision-maker learns and updates his degree of rational beliefs about possible states of nature, in order to make a better judgment while taking new evidence into account. Such a scientific model proposed for the general theory of decision-making, like all others in general, whether in statistics, economics, operations research, A.I., data science or applied mathematics, regardless of whether they are time-dependent, have in common a theoretical basis that is axiomatized by relying on related concepts of a universe of possibles, especially the so-called universe (or the world), the state of nature (or the state of the world), when formulated explicitly. The issue of where to stand as an observer or a decision-maker to reframe such a universe of possibles together with a partition structure of knowledge (i.e. semantic formalisms), including a copy of itself as it was initially while generalizing it, is not addressed. Memory being the substratum, whether human or artificial, wherein everything stands, to date, even the theoretical possibility of such an operation of self-inclusion is prohibited by pure mathematics. We make this blind spot come to light through a counter-example (namely Archimedes’ Eureka experiment) and explore novel theoretical foundations, fitting better with a quantum form than with fuzzy modeling, to deal with more than a reference universe of possibles. This could open up a new path of investigation for the general theory of decision-making, as well as for Artificial Intelligence, often considered as the science of the imitation of human abilities, while being also the science of knowledge representation and the science of concept formation and reasoning.
基金supported by the Investissement d’Avenir program of the French National Agency of Research for the project GENIUS(ANR-11-BTBR-0001_GENIUS)the Institut Carnot Plant2Pro program for the project POTATOCRISPsupported by the ANR project Immunereceptor(ANR-15-CE20-0007).
文摘Since its discovery as a bacterial adaptive immune system and its development for genome editing in eukaryotes,the CRISPR technology has revolutionized plant research and precision crop breeding.The CRISPR toolbox holds great promise in the production of crops with genetic disease resistance to increase agriculture resilience and reduce chemical crop protection with a strong impact on the environment and public health.In this review,we provide an extensive overviewon recent breakthroughs in CRISPR technology,including the newly developed prime editing system that allows precision gene editing in plants.We present how each CRISPR tool can be selected for optimal use in accordance with its specific strengths and limitations,and illustrate how the CRISPR toolbox can foster the development of genetically pathogen-resistant crops for sustainable agriculture.
文摘Modified BCR sequential extraction, single equilibrium-based EDTA extraction and kinetic fractionation were used for estimating the Pb and Cd availability in a series of soil samples from 3 sites located in urban areas of Hanoi (Vietnam). These schemes were compared to identify a simple, rapid and cheap protocol for routine estimation of Pb and Cd remobilizable fraction and the related potential risk. The comparison of these three approaches revealed their convergence in terms of mobility patterns observed for Pb and Cd. Cd was characterized by higher extractibality and mobility whatever the approach. Pb was distinguished of Cd by its high association to Feoxides, lower extractability, lower rate of desorption and then lower mobility. For this environmental scenario, EDTA single scheme at equilibrium could be suggested as the best suited and a simple protocol for determination of the labile pool of Cd and Pb. The pseudo-total concentrations of Pb and Cd are actually below the Vietnamese standard level, contrary to the results obtained by other authors for agricultural soils at other urban sites of Hanoi.
文摘Algae have become an area of intensive research in many fields of study. Areas of application are becoming increasingly diverse with the advent of technologies particularly in the mass production of algae biomass. Algae contain complex bioactive compounds and these are gaining importance in emerging technologies with nutritional and environmental applications. In this study, a preliminary investigation evaluated 15 species of algae from the major categories of marine and fresh water algae for their potential as inclusions in ruminant diets for management of greenhouse gas emissions. It was hypothesized that algae would positively affect rumen fermentation and gas production while reducing methane production. The hypothesis was tested using an Ankom automated gas monitoring system and rumen fluid from Bos indicus steers fed tropical forage diets. The results were variable between algae species with some showing a significant reduction in total gas and methane production, with others increasing gas and fermentation. The red and brown algae stand out as having potential for greenhouse gas mitigation with the brown alga Cystoseira having the most prominent effect. The effects observed on fermentation may be manipulated through dosage management and beneficial effects could be potentially maximized by preparing combinations of algal supplements. It has been demonstrated in this study that algae have the potential to assist in rumen fermentation management for improved gas production, and greenhouse gas abatement.
基金supported by INRAE funding(SCANNER project,BAP Department)the support of Saclay Plant Sciences-SPS(ANR-17-EUR-0007).
文摘Plant defense responses involve several biological processes that allow plants to fight against pathogenic attacks.How these different processes are orchestrated within organs and depend on specific cell types is poorly known.Here,using single-cell RNA sequencing(scRNA-seq)technology on three independent biological replicates,we identified several cell populations representing the core transcriptional responses of wild-type Arabidopsis leaves inoculated with the bacterial pathogen Pseudomonas syringae DC3000.Among these populations,we retrieved major cell types of the leaves(mesophyll,guard,epidermal,companion,and vascular S cells)with which we could associate characteristic transcriptional reprogramming and regulators,thereby specifying different cell-type responses to the pathogen.Further analyses of transcriptional dynamics,on the basis of inference of cell trajectories,indicated that the different cell types,in addition to their characteristic defense responses,can also share similar modules of gene reprogramming,uncovering a ubiquitous antagonism between immune and susceptible processes.Moreover,it appears that the defense responses of vascular S cells,epidermal cells,and mesophyll cells can evolve along two separate paths,one converging toward an identical cell fate,characterized mostly by lignification and detoxification functions.As this divergence does not correspond to the differentiation between immune and susceptible cells,we speculate that this might reflect the discrimination between cellautonomous and non-cell-autonomous responses.Altogether our data provide an upgraded framework to describe,explore,and explain the specialization and the coordination of plant cell responses upon pathogenic challenge.
基金EU grant 874850 MOOD and is catalogued as MOOD009the French General Directorate for Food(DGAL),the French Agricultural Research Centre for International Development(CIRAD),the SONGES Project(FEDER and Occitanie),and the French National Research Agency under the Investments for the Future Program,referred to as ANR-16-CONV-0004(#DigitAg).
文摘Several internet-based surveillance systems have been created to monitor the web for animal health surveillance.These systems collect a large amount of news dealing with outbreaks related to animal diseases.Automatically identifying news articles that describe the same outbreak event is a key step to quickly detect relevant epidemiological information while alleviating manual curation of news content.This paper addresses the task of retrieving news articles that are related in epidemiological terms.We tackle this issue using text mining and feature fusion methods.The main objective of this paper is to identify a textual representation in which two articles that share the same epidemiological content are close.We compared two types of representations(i.e.,features)to represent the documents:(i)morphosyntactic features(i.e.,selection and transformation of all terms from the news,based on classical textual processing steps)and(ii)lexicosemantic features(i.e.,selection,transformation and fusion of epidemiological terms including diseases,hosts,locations and dates).We compared two types of term weighing(i.e.,Boolean and TF-IDF)for both representations.To combine and transform lexicosemantic features,we compared two data fusion techniques(i.e.,early fusion and late fusion)and the effect of features generalisation,while evaluating the relative importance of each type of feature.We conducted our analysis using a corpus composed of a subset of news articles in English related to animal disease outbreaks.Our results showed that the combination of relevant lexicosemantic(epidemiological)features using fusion methods improves classical morphosyntactic representation in the context of disease-related news retrieval.The lexicosemantic representation based on TF-IDF and feature generalisation(F-measure=0.92,r-precision=0.58)outperformed the morphosyntactic representation(F-measure=0.89,r-precision=0.45),while reducing the features space.Converting the features into lower granular features(i.e.,generalisation)contributed to impr
文摘Sugarcane bagasse was pyrolyzed using a laboratory fixed bed reactor to produce char and its by-product(pyrolysis liquid).The pyrolysis experiments were carried out using different temperatures(400℃ and 500℃),heating rate(1℃/min and 10℃/min),and holding time(30 min and 60 min).Char was characterized according to its thermal properties,while the pyrolysis liquid was tested for its anti-fungal and anti-termite activities.Pyrolysis temperature and heating rate had a significant influence on the char properties and the yield of char and pyrolysis liquid,where a high-quality char and high yield of pyrolysis liquid can be obtained at a temperature of 500℃ and a heating rate of 10℃/min.The yield of char and pyrolysis liquid was 28.97%and 55.46%,respectively.The principal compounds of pyrolysis liquid were water,acetic acid,glycolaldehyde,1-hydroxy-2-propanone,methanol,formic acid,levoglucosan,furfural,followed by some phenol compounds and guaiacol derivatives.Pyrolysis liquid at a concentration of 0.20%and 0.25%(v/v)caused a 100%inhibition of Coniophora puteana and Trametes versicolor,respectively,when performing inhibition growth tests in Petri dishes.Filter paper treated with 10%of pyrolysis liquid caused 100%of termite mortality,while only 5.65%–7.03%of the treated filter papers consumed by termites at such concentration.Pyrolysis liquid is potentially effective to be used in the formulation of wood protection against fungi and termites.
基金supported by the Laboratoire d’Excellence(LABEX)TULIP(ANR-10-LABX-41)funded by a grant from the Lebanese University+4 种基金INRAE,Campus France,and the INRAE Plant Health and Environment division(SPE)for their support and fundinga PhD grant co-financed by the Occitanie Regional Council and the INRAE Plant Health and Environment division(SPE)funded by a grant from the French Ministry of National Education and Researchsupported by France Genomique National infrastructurefunded as part of the Investissement d’avenir program managed by Agence Nationale de la Recherche(contract ANR-10-INBS-09).
文摘Quantitative disease resistance(QDR)remains the most prevalent form of plant resistance in crop fields and wild habitats.Genome-wide association studies(GWAS)have proved to be successful in deciphering the quantitative genetic basis of complex traits such as QDR.To unravel the genetics of QDR to the devastating worldwide bacterial pathogen Ralstonia solanacearum,we performed a GWAS by challenging a highly polymorphic local mapping population of Arabidopsis thaliana with four R.solanacearum type III effector(T3E)mutants,identified as key pathogenicity determinants after a first screen on an A.thaliana core collection of 25 accessions.Although most quantitative trait loci(QTLs)were highly specific to the identity of the T3E mutant(ripAC,ripAG,ripAQ,and ripU),we finely mapped a common QTL located on a cluster of nucleotide-binding domain and leucine-rich repeat(NLR)genes that exhibited structural variation.We functionally validated one of these NLRs as a susceptibility factor in response to R.solanacearum,named it Bacterial Wilt Susceptibility 1(BWS1),and cloned two alleles that conferred contrasting levels of QDR.Further characterization indicated that expression of BWS1 leads to suppression of immunity triggered by different R.solanacearum effectors.In addition,we showed a direct interaction between BWS1 and RipAC T3E,and BWS1 and SUPPRESSOR OF G2 ALLELE OF skp1(SGT1b),the latter interaction being suppressed by RipAC.Together,our results highlight a putative role for BWS1 as a quantitative susceptibility factor directly targeted by the T3E RipAC,mediating negative regulation of the SGT1-dependent immune response.
基金This work was supported by the National Natural Science Foundation of China(31971539)the National Science and Technology Basic Resources Survey Program of China(2019FY101700)a scholarship from the China Scholarship Council(202106040062).
文摘The continuation of the isolated Amur tiger(Panthera tigris altaica)population living along the China-Russia border is facing serious challenges due to factors such as its small size(including 38 individuals)and canine distemper virus(CDV).We use a population viability analysis metamodel,which consists of a traditional individual-based demographic model linked to an epidemiological model,to assess options for controlling the impact of negative factors through domestic dog management in protected areas,increasing connectivity to the neighboring large population(including more than 400 individuals),and habitat expansion.Without intervention,under inbreeding depression of 3.14,6.29,and 12.26 lethal equivalents,our metamodel predicted the extinction within 100 years is 64.4%,90.6%,and 99.8%,respectively.In addition,the simulation results showed that dog management or habitat expansion independently will not ensure tiger population viability for the next 100 years,and connectivity to the neighboring population would only keep the population size from rapidly declining.However,when the above three conservation scenarios are combined,even at the highest level of 12.26 lethal equivalents inbreeding depression,population size will not decline and the probability of extinction will be<5.8%.Our findings highlight that protecting the Amur tiger necessitates a multifaceted synergistic effort.Our key management recommendations for this population underline the importance of reducing CDV threats and expanding tiger occupancy to its former range in China,but re-establishing habitat connectivity to the neighboring population is an important long-term objective.
文摘Several research studies have proven that eliciting and predicting the impact of human activity on ecosystem services will be crucial to support stakeholders’ awareness and to decide how to interact with the environment in a more sustainable manner. In this sense, the ecosystems known as road verges are particularly important because of their length and surface at an international scale, and their role in mitigating the damage done by roads. Plant pollination by insects is one of the most important ecosystem services. Because of its nature and the fact that they extend across a variety of landscapes, roadside can contribute to the maintenance of healthy ecosystems, under the condition of adapted management practices. This research is the first attempt to develop a System Dynamics-based aiming to estimate the ecological and economic impact of maintenance on the road verge pollination service in France. Maintenance strategies of road verges are simulated to compare their performance. The results show that there are ways to improve current maintenance strategies in terms of pollination value, but also that the model needs to consider other ecosystem services and synergistic effects that could further affect pollination to obtain more accurate estimations.
基金P.B.was funded by the Horizon Europe project AI4SoilHealth(Grant No.101086179)J.E.Y was funded by the EcoSSSoil Project,Korea Environmental Industry&Technology Institute(KEITI)(Grant No.2019002820004).
文摘Soil erosion is a complex process involving multiple natural and anthropic agents,causing the deterio-ration of multiple components comprising soil health.Here,we provide an estimate of the spatial pat-terns of cropland susceptibility to erosion by sheet and rill,gully,wind,tillage,and root crops harvesting and report the co-occurrence of these processes using a multi-model approach.In addition,to give a global overview of potential future changes,we identify the locations where these multiple concurrent soil erosion processes may be expected to intersect with projected dry/wet climate changes by 2070.Of a modelled 1.48 billion hectares(B ha)of global cropland,our results indicate that 0.56 B ha(-36%of the total area)are highly susceptible(classes 4 and 5)to a single erosion process,0.27 B ha(-18%of the total area)to two processes and 0.02 B ha(1.4%of the total area)to three or more processes.An estimated 0.82 B ha of croplands are susceptible to possible increases in water(0.68 B ha)and wind(0.14 B ha)erosion.We contend that the presented set of estimates represents a basis for enhancing our founda-tional knowledge on the geography of soil erosion at the global scale.The generated insight on multiple erosion processes can be a useful starting point for decision-makers working with ex-post and ex-ante policy evaluation of the UN Sustainable Development Goal 15(Life on Land)activities.Scientifically,this work provides the hitherto most comprehensive assessment of soil erosion risks at the global scale,based on state-of-the-art models.
基金Supported by the Region Auvergne-Rhone-Alpes and FEDER,No.Thématiques émergentes and Pack Ambition Recherchethe French Government IDEX-ISITE Initiative,No.16-IDEX-0001-CAP 20-25the Ministère de la Recherche et de la Technologie,INSERM and University of Clermont Auvergne,No.UMR1071.
文摘BACKGROUND Chronic abdominal pain is the most common cause for gastroenterology consultation and is frequently associated with functional gastrointestinal disorders including irritable bowel syndrome and inflammatory bowel disease. These disorders present similar brain/gut/microbiota trialogue alterations, associated with abnormal intestinal permeability, intestinal dysbiosis and colonic hypersensitivity(CHS). Intestinal dysbiosis can alter colon homeostasis leading to abnormal activation of the innate immunity that promotes CHS, perhaps involving the toll-like receptors(TLRs), which play a central role in innate immunity.AIM To understand the mechanisms between early life event paradigm on intestinal permeability, fecal microbiota composition and CHS development in mice with TLRs expression in colonocytes.METHODS Maternal separation model(NMS) CHS model, which mimics deleterious events in childhood that can induce a wide range of chronic disorders during adulthood were used. Colonic sensitivity of NMS mice was evaluated by colorectal distension(CRD) coupled with intracolonic pressure variation(IPV) measurement. Fecal microbiota composition was analyzed by 16S rRNA sequencing from weaning to CRD periods. TLR mRNA expression was evaluated in colonocytes.Additionally, the effect of acute intrarectal instillation of the TLR5 agonist flagellin(FliC) on CHS in adult naive wildtype mice was analyzed.RESULTS Around 50% of NMS mice exhibited increased intestinal permeability and CHS associated with intestinal dysbiosis, characterized by a significant decrease of species richness, an alteration of the core fecal microbiota and a specific increased relative abundance of flagellated bacteria. Only TLR5mRNA expression was increased in colonocytes of NMS mice with CHS. Acute intrarectal instillation of FliC induced transient increase of IPV, reflecting transient CHS appearance.CONCLUSION Altogether, these data suggest a pathophysiological continuum between intestinal dysbiosis and CHS, with a role for TLR5.
基金We thank Laikun Ma,Tongping Su,and Juan Huo for their assistances.
文摘Although egg color polymorphism has evolved as an effective defensive adaptation to brood parasitism,spatial variations in egg color polymorphism remain poorly characterized.Here,we investigated egg polymorphism in 647 host species(68 families and 231 gen era)parasitized by 41 species of Old Word cuckoos(1 family and 11 gen era)across Asia,Europe,Africa,and Australia.The diversity of parasitic cuckoos differs among continents,reflecting the continent-specific intensities of parasitic selection pressure on hosts.Therefore,host egg polymorphism is expected to evolve more frequently on continents with higher cuckoo diversity.We identified egg polymorphism in 24.1%of all host species and 47.6%of all host families.The common cuckoo Cuculus canorus utilized 184 hosts(28.4%of all host species).Hosts of the common cuckoo and of Chrysococcyx species were more likely to have polymorphic eggs than hosts parasitized by other cuckoos.Both the number of host species and the host families targeted by the cuckoo species were positively correlated with the frequency of host egg polymorphism.Most host species and most hosts exhibiting egg color polymorphism were located in Asia and Africa.Host egg polymorphism was observed less frequently in Australia and Europe.Our results also suggested that egg polymorphism tends to occur more frequently in hosts that are utilized by several cuckoo species or by generalist cuckoo species.We suggest that selecti on pressure on hosts from a given contin ent in creases proportionally to the number of cuckoo species,and that this selection pressure may,in turn,favor the evolution of host egg polymorphism.
基金supported by the Hainan Provincial Natural Science Foundation of China (No.320CXTD437 and 2019RC189 to CY)the National Natural Science Foundation of China (Nos.31772453 and 31970427 to WL)
文摘Egg discrimination by cavity-nesting birds that build nests under dim light conditions was presumed to depend on nest luminance,although this hypothesis has rarely been tested.Tests of egg discrimination ability by cavity-nesting tits under dim light conditions may reveal the selection pressure from brood parasitism that they encounter under natural interactions.We manipulated the intensity of luminance of nests of the Green-backed Tit(Parus monticolus),a potential cuckoo host that possesses a strong discrimination ability of non-mimetic foreign eggs.We performed experiments to test their egg discrimination ability under different light conditions.Our results showed that Green-backed Tits discriminate against non-mimetic foreign eggs under normal light conditions in nest boxes,and this ability persisted at nest luminance as low as 4.78±1.31 lux that is several times lower than normal luminance(38.11±24.02 lux).However,egg discrimination by Green-backed Tits disappeared when nest luminance was reduced to a minimum of 0.35±0.15 lux.The latter value represents total darkness for humans.The present study shows that nest luminance plays a key role in egg discrimination by Green-backed Tits that build nests under dim light conditions.This study provides strong experimental evidence for nest illumination altering egg rejection behavior in cavity-nesting birds.
文摘Intercropping is the planned cultivation of species mixtures on agricultural land.Intercropping has many attributes that make it attractive for developing a more sustainable agriculture,such as high yield,high resource use efficiency,lower input requirements,natural suppression of pests,pathogens and weeds,and building a soil with more organic carbon and nitrogen.Information is needed which species combinations perform best under different circumstances and which management is suitable to bring out the best from intercropping in a given production situation.The literature is replete with case studies on intercropping from across the globe,but evidence synthesis is needed to make this information accessible.Meta-analysis requires a careful choice of metric that is appropriate for answering the question at hand,and which lends itself for a robust meta-analysis.This paper reviews some metrics that may be used in the quantitative synthesis of literature data on intercropping.
