Objective:To establish an ovarian carcinoma multidrug resistance (MDR) cell line in order to explore the multidrug resistant mechanism in ovarian carcinoma and to understand the development of MDR and possible ways to...Objective:To establish an ovarian carcinoma multidrug resistance (MDR) cell line in order to explore the multidrug resistant mechanism in ovarian carcinoma and to understand the development of MDR and possible ways to overcome this phenomenon.Methods:SKOV 3/ADM,a MDR cell line,was established by SKOV 3 parent cells exposed to progressively and intermittently increasing higher concentration of adriamycin (ADM) and had been identified.Results:Besides the drug resistance to adriamycin, the SKOV 3/ADM cells exhibited crossresistant to many other chemotherapeutic agents ,such as VM-26,VP-16 and others.The emergence of drug-resistant SKOV 3/ADM cells was mainly caused by the decreased intracellular drug concentration as a result of overexpression of P-glycoprotein encoded by MDR 1 gene.Conclusion:This cell line showed the typical multidrug resistance phenotypes and might serve as an ideal model for studying the mechanism of MDR.展开更多
妊娠期高血压疾病是全世界范围内导致孕产妇和围产儿死亡的重要原因之一,全球发病率在2%~8%。因其影响范围广泛、不良妊娠结局发生率高以及高昂的医疗花费,妊娠期高血压疾病一直是全球产科医生关注的重点。2019年美国妇产科医师学会(Ame...妊娠期高血压疾病是全世界范围内导致孕产妇和围产儿死亡的重要原因之一,全球发病率在2%~8%。因其影响范围广泛、不良妊娠结局发生率高以及高昂的医疗花费,妊娠期高血压疾病一直是全球产科医生关注的重点。2019年美国妇产科医师学会(American College of Obstetricians and Gynecologists,ACOG)发布了'妊娠期高血压和子痫前期指南2019版”[1],本指南主要针对妊娠期高血压和子痫前期,在2013版指南[2]的基础上加入了最近几年该领域的研究进展,包括疾病的背景信息和处理建议两部分内容。现针对该指南第一部分内容解读如下。展开更多
文摘Objective:To establish an ovarian carcinoma multidrug resistance (MDR) cell line in order to explore the multidrug resistant mechanism in ovarian carcinoma and to understand the development of MDR and possible ways to overcome this phenomenon.Methods:SKOV 3/ADM,a MDR cell line,was established by SKOV 3 parent cells exposed to progressively and intermittently increasing higher concentration of adriamycin (ADM) and had been identified.Results:Besides the drug resistance to adriamycin, the SKOV 3/ADM cells exhibited crossresistant to many other chemotherapeutic agents ,such as VM-26,VP-16 and others.The emergence of drug-resistant SKOV 3/ADM cells was mainly caused by the decreased intracellular drug concentration as a result of overexpression of P-glycoprotein encoded by MDR 1 gene.Conclusion:This cell line showed the typical multidrug resistance phenotypes and might serve as an ideal model for studying the mechanism of MDR.
文摘妊娠期高血压疾病是全世界范围内导致孕产妇和围产儿死亡的重要原因之一,全球发病率在2%~8%。因其影响范围广泛、不良妊娠结局发生率高以及高昂的医疗花费,妊娠期高血压疾病一直是全球产科医生关注的重点。2019年美国妇产科医师学会(American College of Obstetricians and Gynecologists,ACOG)发布了'妊娠期高血压和子痫前期指南2019版”[1],本指南主要针对妊娠期高血压和子痫前期,在2013版指南[2]的基础上加入了最近几年该领域的研究进展,包括疾病的背景信息和处理建议两部分内容。现针对该指南第一部分内容解读如下。
