目的检测7个Y染色体单核苷酸多态性(Y chromosome single nucleotide polymorphism,Y-SNP)位点在内蒙古自治区蒙古族无关男性中的分布,为其法医学应用提供基础数据。方法从HapMap等数据库及相关文献进行筛选,获得在蒙古族中二等位基因...目的检测7个Y染色体单核苷酸多态性(Y chromosome single nucleotide polymorphism,Y-SNP)位点在内蒙古自治区蒙古族无关男性中的分布,为其法医学应用提供基础数据。方法从HapMap等数据库及相关文献进行筛选,获得在蒙古族中二等位基因频率预期接近0.50:0.50的7个Y-SNP,以连接酶检测反应法对95名蒙古族无关男性DNA的7个Y-SNP进行检测,用Arlequin 3.5软件进行统计分型。结果7个Y-SNP均检测到2个等位基因,在95名个体中共检测到7个Y-SNP单倍型,单倍型差异度值为0.7990;rs17316007位点的2个等位基因频率为0.832:0.168、偏离0.50:0.50较多,基因差异度值为0.2825;其余6个Y-SNP位点的2个等位基因的频率均接近0.50:0.50,基因差异度值不低于0.4375。结论在7个Y-SNP中,除rs17316007外,其余6个位点具有较好的基因差异度和遗传多态性,可用于内蒙古地区蒙古族男性的个体识别和亲权鉴定。展开更多
A method for determination of nilestriol in the fish by coupling direct insertion probe with mass spectrometer was established. When the fish be dewatered, nilestriol can be drawed with chloroform and the analysis can...A method for determination of nilestriol in the fish by coupling direct insertion probe with mass spectrometer was established. When the fish be dewatered, nilestriol can be drawed with chloroform and the analysis can be made by coupling direct insertion probe with mass spectrometer as the primary temperature at 100℃ and maintains for 30s, then the temperation rised to 300℃ at the rate of 150℃ · min^-1 and kept for 30s. The ratio of signal to noise(S/N) of nilestriol is 14 : 1 as the sample content is lng. The method issimple in operation and sensitive.展开更多
文摘A method for determination of nilestriol in the fish by coupling direct insertion probe with mass spectrometer was established. When the fish be dewatered, nilestriol can be drawed with chloroform and the analysis can be made by coupling direct insertion probe with mass spectrometer as the primary temperature at 100℃ and maintains for 30s, then the temperation rised to 300℃ at the rate of 150℃ · min^-1 and kept for 30s. The ratio of signal to noise(S/N) of nilestriol is 14 : 1 as the sample content is lng. The method issimple in operation and sensitive.
