It was considered that mounting surroundings played a important role in affecting the quality and yield of silk through mounting comparison test. In view of the lack of good ventilation in rural area,authors pointed o...It was considered that mounting surroundings played a important role in affecting the quality and yield of silk through mounting comparison test. In view of the lack of good ventilation in rural area,authors pointed out that mounting management should be emphasized for a great increase in raw silk rate.展开更多
目的了解湖南省湘西自治州黄牛泰勒虫感染率和遗传变异。方法2018年8月至2019年8月,从湘西自治州凤凰县、花垣县和保靖县采集184份黄牛血液样品,用基于泰勒虫属18S核糖体RNA(18S r RNA)基因的PCR方法对所有血液样品进行检测。将部分阳...目的了解湖南省湘西自治州黄牛泰勒虫感染率和遗传变异。方法2018年8月至2019年8月,从湘西自治州凤凰县、花垣县和保靖县采集184份黄牛血液样品,用基于泰勒虫属18S核糖体RNA(18S r RNA)基因的PCR方法对所有血液样品进行检测。将部分阳性产物基因序列与GenBank数据库中收录的相应虫株序列进行同源性比对,以卵形疟原虫18S rRNA基因作为外群构建种系发育树。结果共143份血液样品检测为泰勒虫阳性,平均检出率为77.7%。凤凰县、花垣县和保靖县黄牛血液样品均检出泰勒虫,检出率分别为85.0%、88.3%和61.0%;湘西黄牛和普通黄牛泰勒虫检出率分别为77.2%和79.5%(χ^(2)=0.08,P>0.05);舍饲牛群与散养牛群检出率分别为68.9%和89.7%(χ^(2)=22.36,P<0.01)。随机挑选18个PCR阳性样品进行测序与序列分析,发现所有样品与已知吕氏泰勒虫分离株同源性超过99.0%;系统进化树分析发现18个样品与吕氏泰勒虫聚为同一分支,但与其他虫株所属分支相隔较远。结论湖南省湘西地区黄牛泰勒虫感染率较高,吕氏泰勒虫可能为优势虫种。展开更多
文摘It was considered that mounting surroundings played a important role in affecting the quality and yield of silk through mounting comparison test. In view of the lack of good ventilation in rural area,authors pointed out that mounting management should be emphasized for a great increase in raw silk rate.
文摘目的了解湖南省湘西自治州黄牛泰勒虫感染率和遗传变异。方法2018年8月至2019年8月,从湘西自治州凤凰县、花垣县和保靖县采集184份黄牛血液样品,用基于泰勒虫属18S核糖体RNA(18S r RNA)基因的PCR方法对所有血液样品进行检测。将部分阳性产物基因序列与GenBank数据库中收录的相应虫株序列进行同源性比对,以卵形疟原虫18S rRNA基因作为外群构建种系发育树。结果共143份血液样品检测为泰勒虫阳性,平均检出率为77.7%。凤凰县、花垣县和保靖县黄牛血液样品均检出泰勒虫,检出率分别为85.0%、88.3%和61.0%;湘西黄牛和普通黄牛泰勒虫检出率分别为77.2%和79.5%(χ^(2)=0.08,P>0.05);舍饲牛群与散养牛群检出率分别为68.9%和89.7%(χ^(2)=22.36,P<0.01)。随机挑选18个PCR阳性样品进行测序与序列分析,发现所有样品与已知吕氏泰勒虫分离株同源性超过99.0%;系统进化树分析发现18个样品与吕氏泰勒虫聚为同一分支,但与其他虫株所属分支相隔较远。结论湖南省湘西地区黄牛泰勒虫感染率较高,吕氏泰勒虫可能为优势虫种。
