利用微卫星标记技术,采用8对微卫星引物对凡纳滨对虾3个亲本群体(OIQ、SISQ、Kona Bay Q)及其子一代(OIZ、SISZ、Kona Bay Z)共计120个个体进行遗传分析。结果表明,8对微卫星引物在6个群体中共检测到28个等位基因,每个位点的等位基因数...利用微卫星标记技术,采用8对微卫星引物对凡纳滨对虾3个亲本群体(OIQ、SISQ、Kona Bay Q)及其子一代(OIZ、SISZ、Kona Bay Z)共计120个个体进行遗传分析。结果表明,8对微卫星引物在6个群体中共检测到28个等位基因,每个位点的等位基因数为2～6个,平均等位基因数为3.5;各位点的期望杂合度均比观测杂合度高;多态信息含量(PIC)值为0.4794～0.7699,说明这8个位点在凡纳滨对虾中具有较高的信息含量。在亲本和子代群体遗传结构分析中,子代的有效等位基因数、杂合度和多态信息含量等指标均略低于亲本群体,但子代的遗传多样性并未受到太大影响,仍保持较好的遗传力。展开更多
Genetic variations of three species from primary parent （OI Q,SIS Q,Kona Bay Q） and their first filial generation （OI Z,SIS Z,Kona Bay Z） of Litopenaeus vannamei were detected using microsatellite marker technique...Genetic variations of three species from primary parent （OI Q,SIS Q,Kona Bay Q） and their first filial generation （OI Z,SIS Z,Kona Bay Z） of Litopenaeus vannamei were detected using microsatellite marker technique.The amplified products of genomic DNA with 8 microsatellite makers indicated that 8 loci presents polymorphism,the number of total alleles were 28 in 6 species;the number of allele of every loci was 2 to 6;mean allele number （Na） was 3.5;expected heterozygosity value （He） were higher than observed heterozygosity value （Ho）;mean polymorphism information content （PIC） ranged from 0.479 4 to 0.769 9,which showed these 8 loci posses relatively high information content.Through analysis of genetic structure in primary parent and their first filial,allele and effective allele number,observed heterozygosity and expected heterozygosity values and mean polymorphism information content （PIC） in filial generation were little lower than their primary parents.However,genetic diversity of filial generation is not influenced,and still maintains a good heritability.展开更多
文摘利用微卫星标记技术,采用8对微卫星引物对凡纳滨对虾3个亲本群体(OIQ、SISQ、Kona Bay Q)及其子一代(OIZ、SISZ、Kona Bay Z)共计120个个体进行遗传分析。结果表明,8对微卫星引物在6个群体中共检测到28个等位基因,每个位点的等位基因数为2～6个,平均等位基因数为3.5;各位点的期望杂合度均比观测杂合度高;多态信息含量(PIC)值为0.4794～0.7699,说明这8个位点在凡纳滨对虾中具有较高的信息含量。在亲本和子代群体遗传结构分析中,子代的有效等位基因数、杂合度和多态信息含量等指标均略低于亲本群体,但子代的遗传多样性并未受到太大影响,仍保持较好的遗传力。
基金Supported by Technology Innovation Projects from Science and Tech-nology Department of Guangdong Province (2008A023700011)~~
文摘Genetic variations of three species from primary parent （OI Q,SIS Q,Kona Bay Q） and their first filial generation （OI Z,SIS Z,Kona Bay Z） of Litopenaeus vannamei were detected using microsatellite marker technique.The amplified products of genomic DNA with 8 microsatellite makers indicated that 8 loci presents polymorphism,the number of total alleles were 28 in 6 species;the number of allele of every loci was 2 to 6;mean allele number （Na） was 3.5;expected heterozygosity value （He） were higher than observed heterozygosity value （Ho）;mean polymorphism information content （PIC） ranged from 0.479 4 to 0.769 9,which showed these 8 loci posses relatively high information content.Through analysis of genetic structure in primary parent and their first filial,allele and effective allele number,observed heterozygosity and expected heterozygosity values and mean polymorphism information content （PIC） in filial generation were little lower than their primary parents.However,genetic diversity of filial generation is not influenced,and still maintains a good heritability.
