本研究运用RT-PCR技术,首次从大熊猫(Ailuropoda melanoleuca)的肌肉组织总RNA中成功克隆了RPS24(Ribosomal Protein S24)基因的表达序列,并对其进行了测序及初步分析.结果表明:大熊猫RPS24基因的表达序列全长为431bp,开放阅读框(ORF)为...本研究运用RT-PCR技术,首次从大熊猫(Ailuropoda melanoleuca)的肌肉组织总RNA中成功克隆了RPS24(Ribosomal Protein S24)基因的表达序列,并对其进行了测序及初步分析.结果表明:大熊猫RPS24基因的表达序列全长为431bp,开放阅读框(ORF)为399bp,编码132个氨基酸的蛋白质,该蛋白的分子量为15.3251kD,pI为10.92,含有7种类型共14个功能位点:即1个N-糖基化位点、2个cAMP和cGMP依赖性蛋白激酶磷酸化位点、6个蛋白激酶C磷酸化位点、1个酪蛋白激酶Ⅱ磷酸化位点、3个N-酰基化位点、1个酰胺化位点及1个RPS24e sig-nature.进一步分析发现,大熊猫RPS24基因与已报道的人、牛、苏门答腊猩猩、褐家鼠和小家鼠5个哺乳动物物种的表达序列及其编码的氨基酸序列具有很高的相似性:编码序列同源性分别为94.1%、92.4%、94.7%、89.9%和90.4%;与人RPS24蛋白的氨基酸序列同源性为98.48%,其余均为99.24%.展开更多
The thermooxidative degradation of methyl methacry late-graft-natura l rubber(MG) in air was studied by Thermogravimetric Analysis(TGA). The results indicate that the thermooxidative degradation of MG-30 in air has on...The thermooxidative degradation of methyl methacry late-graft-natura l rubber(MG) in air was studied by Thermogravimetric Analysis(TGA). The results indicate that the thermooxidative degradation of MG-30 in air has one weight lo ss step change on the whole. The degradation temperatures and the degradation ra tes increase linearly along with the increment of heating rates. The temperature of initial degradation ( t 0) is 0 667 B +351 7 ℃, the temperature at maximum deg radation rate ( t p) is 0 923+385 4 ℃, and the temperature of final degra dation ( t f) is 0 923+385 4 ℃. The degradation rate at t p is not affected by B and its a verage value is 46 7%. However, the degradation rate at t f gains linearly with the increase of B and its average value is 93 0%.展开更多
文摘本研究运用RT-PCR技术,首次从大熊猫(Ailuropoda melanoleuca)的肌肉组织总RNA中成功克隆了RPS24(Ribosomal Protein S24)基因的表达序列,并对其进行了测序及初步分析.结果表明:大熊猫RPS24基因的表达序列全长为431bp,开放阅读框(ORF)为399bp,编码132个氨基酸的蛋白质,该蛋白的分子量为15.3251kD,pI为10.92,含有7种类型共14个功能位点:即1个N-糖基化位点、2个cAMP和cGMP依赖性蛋白激酶磷酸化位点、6个蛋白激酶C磷酸化位点、1个酪蛋白激酶Ⅱ磷酸化位点、3个N-酰基化位点、1个酰胺化位点及1个RPS24e sig-nature.进一步分析发现,大熊猫RPS24基因与已报道的人、牛、苏门答腊猩猩、褐家鼠和小家鼠5个哺乳动物物种的表达序列及其编码的氨基酸序列具有很高的相似性:编码序列同源性分别为94.1%、92.4%、94.7%、89.9%和90.4%;与人RPS24蛋白的氨基酸序列同源性为98.48%,其余均为99.24%.
文摘The thermooxidative degradation of methyl methacry late-graft-natura l rubber(MG) in air was studied by Thermogravimetric Analysis(TGA). The results indicate that the thermooxidative degradation of MG-30 in air has one weight lo ss step change on the whole. The degradation temperatures and the degradation ra tes increase linearly along with the increment of heating rates. The temperature of initial degradation ( t 0) is 0 667 B +351 7 ℃, the temperature at maximum deg radation rate ( t p) is 0 923+385 4 ℃, and the temperature of final degra dation ( t f) is 0 923+385 4 ℃. The degradation rate at t p is not affected by B and its a verage value is 46 7%. However, the degradation rate at t f gains linearly with the increase of B and its average value is 93 0%.
