血管内皮生长因子(vascular endothelial growth factor,VEGF)可诱导血管生成,对肿瘤的生长和转移至关重要。目前,VEGF已成为新药研发的主要靶标,这类新药可通过阻断微血管的形成和生长达到抑制肿瘤细胞的生长或是维持肿瘤处于休眠期的...血管内皮生长因子(vascular endothelial growth factor,VEGF)可诱导血管生成,对肿瘤的生长和转移至关重要。目前,VEGF已成为新药研发的主要靶标,这类新药可通过阻断微血管的形成和生长达到抑制肿瘤细胞的生长或是维持肿瘤处于休眠期的目的。该研究制备了中和性VEGF单克隆抗体(monoclonal antibody,Mc Ab)并探讨其对肺癌裸鼠模型血管生成的抑制作用。利用可编码VEGF全长蛋白的重组质粒免疫Balb/c小鼠,获得中和性VEGF Mc Ab,并利用VEGF±VEGF Mc Ab诱导人脐静脉内皮细胞(human umbilical vein endothelial cells,Hu VEC)。通过Western blot法检测VEGFR-2磷酸化水平(活化状态),进而测定VEGF Mc Ab的中和性。VEGF Mc Ab可以抑制VEGF对VEGFR-2酪氨酸磷酸化的激活作用。该研究将A549肺癌细胞接种于裸鼠构建肿瘤模型,探讨了VEGF Mc Ab抗血管生成的效果。结果表明,50μg VEGF Mc Ab治疗组动物模型的瘤重抑制率为51.5%,瘤体抑制率为62.5%。VEGF Mc Ab不仅可以抑制VEGF对人脐静脉内皮细胞VEGFR-2介导的信号转导作用,且可有效抑制小鼠肺癌模型中肿瘤的生长。展开更多
Noncoding RNA(ncRNA) is a kind of transcripts without the ability of coding proteins,which exist widely in highly eukaryotic.The amount of the ncRNA transcripts is up to 98% of the whole genome’s transcripts.The diff...Noncoding RNA(ncRNA) is a kind of transcripts without the ability of coding proteins,which exist widely in highly eukaryotic.The amount of the ncRNA transcripts is up to 98% of the whole genome’s transcripts.The difference in molecular structure and expressive pattern from other kinds of RNA endows them specific biological functions.Although exact functions of most ncRNA are not well known,it has been demonstrated that ncRNA was associated with some major human diseases.It would be helpful to design effective therapeutic strategies,if we understand how ncRNA is involved in the pathogenesis of human major diseases.This review will shed light on the expressive pattern and distribution of ncRNA,the relationship between diseases and ncRNA and finally the application of ncRNA in the therapeutic fields.展开更多
蒽贝素(Embelin)是一种X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)的小分子抑制剂,可以抑制XIAP的生成和活性,从而解除XIAP的抗凋亡作用,使凋亡顺利进行.研究了Embelin抑制肝癌细胞Bel-7404增殖的机制.采用不同...蒽贝素(Embelin)是一种X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)的小分子抑制剂,可以抑制XIAP的生成和活性,从而解除XIAP的抗凋亡作用,使凋亡顺利进行.研究了Embelin抑制肝癌细胞Bel-7404增殖的机制.采用不同浓度梯度,通过荧光显微镜、Hochest33342染色、MTT检测、AnnexinⅤ/PI流式细胞术和Western blot分别检测Embelin对Bel-7404细胞的形态学变化、凋亡小体形成、细胞存活率、细胞凋亡水平、凋亡信号转导相关蛋白表达的影响.结果显示,Embelin能明显抑制Bel-7404细胞增殖,并通过激活caspase通路以及阻断NF-κB信号通路诱导Bel-7404细胞凋亡,为进一步利用Embelin进行肝癌治疗的研究打下一定的基础.展开更多
文摘血管内皮生长因子(vascular endothelial growth factor,VEGF)可诱导血管生成,对肿瘤的生长和转移至关重要。目前,VEGF已成为新药研发的主要靶标,这类新药可通过阻断微血管的形成和生长达到抑制肿瘤细胞的生长或是维持肿瘤处于休眠期的目的。该研究制备了中和性VEGF单克隆抗体(monoclonal antibody,Mc Ab)并探讨其对肺癌裸鼠模型血管生成的抑制作用。利用可编码VEGF全长蛋白的重组质粒免疫Balb/c小鼠,获得中和性VEGF Mc Ab,并利用VEGF±VEGF Mc Ab诱导人脐静脉内皮细胞(human umbilical vein endothelial cells,Hu VEC)。通过Western blot法检测VEGFR-2磷酸化水平(活化状态),进而测定VEGF Mc Ab的中和性。VEGF Mc Ab可以抑制VEGF对VEGFR-2酪氨酸磷酸化的激活作用。该研究将A549肺癌细胞接种于裸鼠构建肿瘤模型,探讨了VEGF Mc Ab抗血管生成的效果。结果表明,50μg VEGF Mc Ab治疗组动物模型的瘤重抑制率为51.5%,瘤体抑制率为62.5%。VEGF Mc Ab不仅可以抑制VEGF对人脐静脉内皮细胞VEGFR-2介导的信号转导作用,且可有效抑制小鼠肺癌模型中肿瘤的生长。
文摘Noncoding RNA(ncRNA) is a kind of transcripts without the ability of coding proteins,which exist widely in highly eukaryotic.The amount of the ncRNA transcripts is up to 98% of the whole genome’s transcripts.The difference in molecular structure and expressive pattern from other kinds of RNA endows them specific biological functions.Although exact functions of most ncRNA are not well known,it has been demonstrated that ncRNA was associated with some major human diseases.It would be helpful to design effective therapeutic strategies,if we understand how ncRNA is involved in the pathogenesis of human major diseases.This review will shed light on the expressive pattern and distribution of ncRNA,the relationship between diseases and ncRNA and finally the application of ncRNA in the therapeutic fields.
