The effect of culture media,temperature and humidity on the mycelium growth,spores germination of Metarhizium MS01 strain,a high virulent strain from the soil and the pathogenicity against Anoplophora glabripennis lar...The effect of culture media,temperature and humidity on the mycelium growth,spores germination of Metarhizium MS01 strain,a high virulent strain from the soil and the pathogenicity against Anoplophora glabripennis larvae was tested. The result showed that the optimum culture medium was PPDA,the optimum temperature for the mycelium growth and spore germination was 26 ℃,and A. glabripennis larvae had the highest mortality from the fungus at this temperature. The optimum RH for the mycelium growth ranged from 95%to 100%. The speed and rate of spore germination increased with increasing of RH. The larvae died from the fugus most quickly and the mortality reached the highest under 100% RH.展开更多
According to the morphological taxonomy,the bethylid,which was regarded as Scleroderma guani Xiao et Wu,1983 and used widely for the control of cerambycid larvae in forest of China,was revised as junior synonym of Scl...According to the morphological taxonomy,the bethylid,which was regarded as Scleroderma guani Xiao et Wu,1983 and used widely for the control of cerambycid larvae in forest of China,was revised as junior synonym of Sclerodermus harmandi (Buysson,1903).Unfortunately,we could not find the type of S.guani,so we could not know whether the bethylid used in forest is the same as the type of S.guani or not.展开更多
以中华蜜蜂13个不同日龄的工蜂头部为材料,提取总RNA,经mRNA分离纯化,反转录合成双链cDNA,以pBluescript II SK(+)为载体,构建成中蜂头部cDNA文库。该文库的滴度为8×104pfu/mL,插入片段长500～3000bp,重组频率在90%以上,表明文库...以中华蜜蜂13个不同日龄的工蜂头部为材料,提取总RNA,经mRNA分离纯化,反转录合成双链cDNA,以pBluescript II SK(+)为载体,构建成中蜂头部cDNA文库。该文库的滴度为8×104pfu/mL,插入片段长500～3000bp,重组频率在90%以上,表明文库容量好、重组率高、插入片段较大,为规模化EST测序,并进一步研制基因芯片奠定了基础。展开更多
目的:建立大头金蝇(Chrysomyia megacephala,C.megacephala)成虫头壳蝶啶含量的分析方法。方法:取C.mega-cephala头壳于3 mL Tris-HCl缓冲液(pH 8.0)中匀浆,离心,应用荧光分光光度计分析上清液的荧光强度。结果:当蝶啶溶液浓度低于0.8...目的:建立大头金蝇(Chrysomyia megacephala,C.megacephala)成虫头壳蝶啶含量的分析方法。方法:取C.mega-cephala头壳于3 mL Tris-HCl缓冲液(pH 8.0)中匀浆,离心,应用荧光分光光度计分析上清液的荧光强度。结果:当蝶啶溶液浓度低于0.8个头壳/3 mL时,其荧光强度随溶液浓度的增长呈线性增加(r2=0.9907,P<0.01)。浓度较高时,蝶啶溶液对其荧光有着显著的浓度淬灭效应,其荧光强度随溶液浓度增加逐渐降低(P<0.01)。光照条件下,蝶啶溶液的荧光强度随放置时间的延长而显著增加(r2=0.9676,P<0.01);黑暗条件下,虽然蝶啶溶液的荧光强度也随时间的延长而显著增加(P<0.05),但其增长速率比光照条件下小得多。结论:在进行蝶啶荧光分析时,必须控制蝶啶溶液的终浓度,注意避光,并缩短从匀浆到荧光分析的时间间隔,以减少分析误差。展开更多
文摘The effect of culture media,temperature and humidity on the mycelium growth,spores germination of Metarhizium MS01 strain,a high virulent strain from the soil and the pathogenicity against Anoplophora glabripennis larvae was tested. The result showed that the optimum culture medium was PPDA,the optimum temperature for the mycelium growth and spore germination was 26 ℃,and A. glabripennis larvae had the highest mortality from the fungus at this temperature. The optimum RH for the mycelium growth ranged from 95%to 100%. The speed and rate of spore germination increased with increasing of RH. The larvae died from the fugus most quickly and the mortality reached the highest under 100% RH.
文摘According to the morphological taxonomy,the bethylid,which was regarded as Scleroderma guani Xiao et Wu,1983 and used widely for the control of cerambycid larvae in forest of China,was revised as junior synonym of Sclerodermus harmandi (Buysson,1903).Unfortunately,we could not find the type of S.guani,so we could not know whether the bethylid used in forest is the same as the type of S.guani or not.
文摘以中华蜜蜂13个不同日龄的工蜂头部为材料,提取总RNA,经mRNA分离纯化,反转录合成双链cDNA,以pBluescript II SK(+)为载体,构建成中蜂头部cDNA文库。该文库的滴度为8×104pfu/mL,插入片段长500～3000bp,重组频率在90%以上,表明文库容量好、重组率高、插入片段较大,为规模化EST测序,并进一步研制基因芯片奠定了基础。
