In this paper, the mimetic biomineralization of CaC2O4 in lecithin water(PC H2O) ordered systems has been discussed. First, the effect of the aggregation state of PC aqueous solutions on the crystal structure of CaC2O...In this paper, the mimetic biomineralization of CaC2O4 in lecithin water(PC H2O) ordered systems has been discussed. First, the effect of the aggregation state of PC aqueous solutions on the crystal structure of CaC2O4 has been studied. In all experiments, the only precipitated crystal forms obtained are calcium oxalate monohydrate(COM) and calcium oxalate dihydrate(COD). It have been shown that the relative amount of COD in PC H2O systems is much higher than that of in H2O system, and furthermore the aggregation state of PC influences the composition of coprecipitations to some extent. When PC distributing in aqueous solution as single molecules, it acts as inhibitor of COM , and then favors the formation of COD. While PC in micelle existence, the relative amount of COM increases , but is still lower than that of in H2O system . And when PC aggregates as vesicles, COD forms predominantly. Second, the morphology of CaC2O4 obtained in different reaction systems under different conditions has been also investigated. In H2O system, we observed thin, plate like crystals with serrated edges underISL(low ionic strength) condition and oriented clusters under ISH(high ionic strength) condition. Crystals with two layers arrangement were obtained in micelleordered PC H2O system under ISL condition. And in vesicle ordered PC H2O system, clusters with butterfly like shape were observed. The results obtained revealed a complex dependence of calcium oxalate formation,crystal growth, structure and morphology on the aggregation state of PC H2O systems.展开更多
A novel Hadamard transform(HT) microscopic image detection system which can generate 511× 512 pixel format images of fluorescence or absorptive light for small samples has been developed, when spatial multiplexin...A novel Hadamard transform(HT) microscopic image detection system which can generate 511× 512 pixel format images of fluorescence or absorptive light for small samples has been developed, when spatial multiplexing is performed with a 511 slit one dimensional Hadamard mask and a highly sensitive linear CCD. The weak native fluorescence imaging for Zephyranthes candida (Lindl.) Herb. Pollen cells can be realized within 1 min. The spatial resolution of 0 6 μm per pixel has been achieved with a 40× objective. The imaging speed, sensitivity and spatial resolution are satisfactory, hence the system has a good application prospect in cell biology and medicine. Some factors that influence imaging speed and quality have been discussed preliminarily.展开更多
The imaging capability(sensitivity, signal-to-noise ratio, imaging speed and spatial resolution) of a domestic-made high-resolution Hadamrd transform(HT) microscopic fluorescence imaging system was discussed. The resu...The imaging capability(sensitivity, signal-to-noise ratio, imaging speed and spatial resolution) of a domestic-made high-resolution Hadamrd transform(HT) microscopic fluorescence imaging system was discussed. The results show that the system can be applied for high-resolution single-cell imaging sensitively in a short time, the cell image provided by the system has a high-resolution up to 511×512 pixel. The results also shows that this system has a more excellent resolving power than conventional optical microscope, under a 100× objective(oil lens) the spatial resolution up to 0.24 μm per pixel in X direction was obtained, which is close to the resolution limit of conventional optical microscope. The system was used for multi-parameter evaluation of breast tumor malignancy and malignant grade for a single tumor cell based on nuclei DNA ploidy measurements, and satisfactory results were obtained.展开更多
文摘In this paper, the mimetic biomineralization of CaC2O4 in lecithin water(PC H2O) ordered systems has been discussed. First, the effect of the aggregation state of PC aqueous solutions on the crystal structure of CaC2O4 has been studied. In all experiments, the only precipitated crystal forms obtained are calcium oxalate monohydrate(COM) and calcium oxalate dihydrate(COD). It have been shown that the relative amount of COD in PC H2O systems is much higher than that of in H2O system, and furthermore the aggregation state of PC influences the composition of coprecipitations to some extent. When PC distributing in aqueous solution as single molecules, it acts as inhibitor of COM , and then favors the formation of COD. While PC in micelle existence, the relative amount of COM increases , but is still lower than that of in H2O system . And when PC aggregates as vesicles, COD forms predominantly. Second, the morphology of CaC2O4 obtained in different reaction systems under different conditions has been also investigated. In H2O system, we observed thin, plate like crystals with serrated edges underISL(low ionic strength) condition and oriented clusters under ISH(high ionic strength) condition. Crystals with two layers arrangement were obtained in micelleordered PC H2O system under ISL condition. And in vesicle ordered PC H2O system, clusters with butterfly like shape were observed. The results obtained revealed a complex dependence of calcium oxalate formation,crystal growth, structure and morphology on the aggregation state of PC H2O systems.
文摘A novel Hadamard transform(HT) microscopic image detection system which can generate 511× 512 pixel format images of fluorescence or absorptive light for small samples has been developed, when spatial multiplexing is performed with a 511 slit one dimensional Hadamard mask and a highly sensitive linear CCD. The weak native fluorescence imaging for Zephyranthes candida (Lindl.) Herb. Pollen cells can be realized within 1 min. The spatial resolution of 0 6 μm per pixel has been achieved with a 40× objective. The imaging speed, sensitivity and spatial resolution are satisfactory, hence the system has a good application prospect in cell biology and medicine. Some factors that influence imaging speed and quality have been discussed preliminarily.
文摘The imaging capability(sensitivity, signal-to-noise ratio, imaging speed and spatial resolution) of a domestic-made high-resolution Hadamrd transform(HT) microscopic fluorescence imaging system was discussed. The results show that the system can be applied for high-resolution single-cell imaging sensitively in a short time, the cell image provided by the system has a high-resolution up to 511×512 pixel. The results also shows that this system has a more excellent resolving power than conventional optical microscope, under a 100× objective(oil lens) the spatial resolution up to 0.24 μm per pixel in X direction was obtained, which is close to the resolution limit of conventional optical microscope. The system was used for multi-parameter evaluation of breast tumor malignancy and malignant grade for a single tumor cell based on nuclei DNA ploidy measurements, and satisfactory results were obtained.
