用微卫星和扩增片段长度多态性（amplmed fragment lengh polymorphism，AFLP）分子标记对海南、广州和青岛吉富品系尼罗罗非鱼的遗传多样性和遗传分化进行了研究。9对微卫星引物和4对AFLP引物的分析结果一致。微卫星分析表明，青岛吉...用微卫星和扩增片段长度多态性（amplmed fragment lengh polymorphism，AFLP）分子标记对海南、广州和青岛吉富品系尼罗罗非鱼的遗传多样性和遗传分化进行了研究。9对微卫星引物和4对AFLP引物的分析结果一致。微卫星分析表明，青岛吉富鱼的平均等位基因数（4．8）、平均观测杂合度（0．528）、平均多态信息含量（0．605）最高，海南吉富鱼的平均等位基因数（4．4）、平均观测杂合度（0．479）、平均多态信息含量（0．549）最低。AFLP分析显示，青岛吉富鱼的多态位点比例（48．4％）和基因多样性（0．245）最高，海南吉富鱼的多态位点比例（36．3％）和基因多样性（0．147）最低。这些表明，青岛吉富鱼的遗传多样性最高，海南吉富鱼最低。遗传分化分析表明，两两群体间遗传分化显著（微卫星以，为0．07—0．11，P〈0．01；AFLP峙为0．24—0．29，P〈0．01）。AMOVA分析显示，大部分遗传变异（微卫星的分析结果为91．26％；AFLP的为67．6％）来源于群体内个体间，表明吉富鱼选育品系尚具有进一步选育的潜力。展开更多
The full length cDNA encoding growth hormone of a freshwater fish, Lepomis cyanellus, (LcGH) was cloned from pituitary RNA with RT-PCR, 3′and 5′ RACE (rapid amplification of cDNA ends). The LcGH cDNA (Genbank No. AY...The full length cDNA encoding growth hormone of a freshwater fish, Lepomis cyanellus, (LcGH) was cloned from pituitary RNA with RT-PCR, 3′and 5′ RACE (rapid amplification of cDNA ends). The LcGH cDNA (Genbank No. AY530822), about 989nt (nucleotide) long, consisted of a open reading frame with 615nt long, 5′and 3′untranslated regions with 93nt and 224nt long respectively, and a 57nt poly (A) tail. The DNA sequence analysis showed that there are typical Kozak sequence and polyadenylation signal. The pregrowth hormone peptide of 204aa deduced from LcGH cDNA included a putative signal peptide (17aa) locating in its N-terminal. There exist a Asn-Cys-Thr glycosylation site at amino acid 201, and 4 cysteine residues (No. 69, 177, 194, 202) that are essential to construct two S-S bonds in this pregrowth hormone peptide. Homological comparision among LcGH and other species growth hormones showed that There is high homology (more than 85%) between growth hormone of Lepomis cyanellus and that of most perciformes fish, but low homology (less than 70%) in comparison with other species such as Siluriformes and Cypriniformes fish.展开更多
对不同来源的养殖群体进行种质鉴定,探索在种苗阶段即区分新吉富罗非鱼(New GIFT strainOreochromis niloticus)、吉诺玛罗非鱼(GenoMar strainO.niloticus)和奥杂罗非鱼(O.niloticus♀×O.aureus♂)3个养殖群体具有重要的意义。笔...对不同来源的养殖群体进行种质鉴定,探索在种苗阶段即区分新吉富罗非鱼(New GIFT strainOreochromis niloticus)、吉诺玛罗非鱼(GenoMar strainO.niloticus)和奥杂罗非鱼(O.niloticus♀×O.aureus♂)3个养殖群体具有重要的意义。笔者选取了5对AFLP(amplified fragment length polymorphism)引物组合对3个罗非鱼养殖群体共60尾个体进行比较分析,引物组合E-ACA/M-CAT表现出较好的区分能力,有7条标记的分布在群体间表现出"有和无"的差别,引物组合E-ACA/M-CAG的扩增图谱中有2条带表现出"有和无"的差别。遗传多样性方面,平均基因多样性在新吉富罗非鱼和吉诺玛罗非鱼分别为0.1605和0.1595,而在奥尼罗非鱼为0.2214,多态位点百分数和Shannnon多样性指数也表现出相近的变化趋势。分析表明新吉富罗非鱼群体在遗传上已较为稳定,吉诺玛罗非鱼遗传多样性偏低。展开更多
文摘用微卫星和扩增片段长度多态性（amplmed fragment lengh polymorphism，AFLP）分子标记对海南、广州和青岛吉富品系尼罗罗非鱼的遗传多样性和遗传分化进行了研究。9对微卫星引物和4对AFLP引物的分析结果一致。微卫星分析表明，青岛吉富鱼的平均等位基因数（4．8）、平均观测杂合度（0．528）、平均多态信息含量（0．605）最高，海南吉富鱼的平均等位基因数（4．4）、平均观测杂合度（0．479）、平均多态信息含量（0．549）最低。AFLP分析显示，青岛吉富鱼的多态位点比例（48．4％）和基因多样性（0．245）最高，海南吉富鱼的多态位点比例（36．3％）和基因多样性（0．147）最低。这些表明，青岛吉富鱼的遗传多样性最高，海南吉富鱼最低。遗传分化分析表明，两两群体间遗传分化显著（微卫星以，为0．07—0．11，P〈0．01；AFLP峙为0．24—0．29，P〈0．01）。AMOVA分析显示，大部分遗传变异（微卫星的分析结果为91．26％；AFLP的为67．6％）来源于群体内个体间，表明吉富鱼选育品系尚具有进一步选育的潜力。
文摘The full length cDNA encoding growth hormone of a freshwater fish, Lepomis cyanellus, (LcGH) was cloned from pituitary RNA with RT-PCR, 3′and 5′ RACE (rapid amplification of cDNA ends). The LcGH cDNA (Genbank No. AY530822), about 989nt (nucleotide) long, consisted of a open reading frame with 615nt long, 5′and 3′untranslated regions with 93nt and 224nt long respectively, and a 57nt poly (A) tail. The DNA sequence analysis showed that there are typical Kozak sequence and polyadenylation signal. The pregrowth hormone peptide of 204aa deduced from LcGH cDNA included a putative signal peptide (17aa) locating in its N-terminal. There exist a Asn-Cys-Thr glycosylation site at amino acid 201, and 4 cysteine residues (No. 69, 177, 194, 202) that are essential to construct two S-S bonds in this pregrowth hormone peptide. Homological comparision among LcGH and other species growth hormones showed that There is high homology (more than 85%) between growth hormone of Lepomis cyanellus and that of most perciformes fish, but low homology (less than 70%) in comparison with other species such as Siluriformes and Cypriniformes fish.
文摘对不同来源的养殖群体进行种质鉴定,探索在种苗阶段即区分新吉富罗非鱼(New GIFT strainOreochromis niloticus)、吉诺玛罗非鱼(GenoMar strainO.niloticus)和奥杂罗非鱼(O.niloticus♀×O.aureus♂)3个养殖群体具有重要的意义。笔者选取了5对AFLP(amplified fragment length polymorphism)引物组合对3个罗非鱼养殖群体共60尾个体进行比较分析,引物组合E-ACA/M-CAT表现出较好的区分能力,有7条标记的分布在群体间表现出"有和无"的差别,引物组合E-ACA/M-CAG的扩增图谱中有2条带表现出"有和无"的差别。遗传多样性方面,平均基因多样性在新吉富罗非鱼和吉诺玛罗非鱼分别为0.1605和0.1595,而在奥尼罗非鱼为0.2214,多态位点百分数和Shannnon多样性指数也表现出相近的变化趋势。分析表明新吉富罗非鱼群体在遗传上已较为稳定,吉诺玛罗非鱼遗传多样性偏低。
