[Objective] The aim of this study is to investigate the chromosome pairing of F1 from Aegilops Ventricosa-Aegilops Cylindrica amphiploid × common wheat.[Method] Microsporogenesis and pollen development of Yannong...[Objective] The aim of this study is to investigate the chromosome pairing of F1 from Aegilops Ventricosa-Aegilops Cylindrica amphiploid × common wheat.[Method] Microsporogenesis and pollen development of Yannong15,SDAU18 and their hybrid F1 were observed cytologically by squash method.[Result] The results showed that microsporogenesis and pollen development of two parents including Yannong15 and SDAU18 were basically normal,and their seed setting was also basically normal.However,the microsporogenesis of their hybrid F1 was chaotic.Univalent and bivalent with high frequency of 10.11 and 18.29 per cell respectively occurred in meiotic MI（metphaseI） of PMC（pollen mother cell）.Nevertheless,only quite low frequency of multivalents occurred in meiotic PMC MI.The laggards at meiotic AI（anaphaseI） were 5 per cell on average.The number of micronucleoli in tetrad was 4.43 per cell averagely.The aborted pollen with different frequency occurred at every stage of hybrid F1 pollen development but mainly after single nucleus pollen stage.The fertile pollen rate of hybrid F1 was only 31% at 3-nucleated stage.[Conclusion] This study provides cytogenetics basis for further analysis and utilization of the amphiploid.展开更多
基金Supported by Doctor Scientific Research Fund from Zaozhuang University~~
文摘[Objective] The aim of this study is to investigate the chromosome pairing of F1 from Aegilops Ventricosa-Aegilops Cylindrica amphiploid × common wheat.[Method] Microsporogenesis and pollen development of Yannong15,SDAU18 and their hybrid F1 were observed cytologically by squash method.[Result] The results showed that microsporogenesis and pollen development of two parents including Yannong15 and SDAU18 were basically normal,and their seed setting was also basically normal.However,the microsporogenesis of their hybrid F1 was chaotic.Univalent and bivalent with high frequency of 10.11 and 18.29 per cell respectively occurred in meiotic MI（metphaseI） of PMC（pollen mother cell）.Nevertheless,only quite low frequency of multivalents occurred in meiotic PMC MI.The laggards at meiotic AI（anaphaseI） were 5 per cell on average.The number of micronucleoli in tetrad was 4.43 per cell averagely.The aborted pollen with different frequency occurred at every stage of hybrid F1 pollen development but mainly after single nucleus pollen stage.The fertile pollen rate of hybrid F1 was only 31% at 3-nucleated stage.[Conclusion] This study provides cytogenetics basis for further analysis and utilization of the amphiploid.
