将草莓分别进行壳聚糖1%-柠檬酸2%、壳聚糖1%-柠檬酸3%、壳聚糖1%-柠檬酸4%、空白对照4种方式涂膜处理,于4℃下贮藏5 d,对其采后生理指标(颜色、硬度、腐烂率、可溶性固形物、呼吸强度、过氧化物酶和多酚氧化酶活性等)进行测定。结果表...将草莓分别进行壳聚糖1%-柠檬酸2%、壳聚糖1%-柠檬酸3%、壳聚糖1%-柠檬酸4%、空白对照4种方式涂膜处理,于4℃下贮藏5 d,对其采后生理指标(颜色、硬度、腐烂率、可溶性固形物、呼吸强度、过氧化物酶和多酚氧化酶活性等)进行测定。结果表明:壳聚糖涂膜能减缓草莓贮藏过程中的褐变程度,延缓草莓果肉的软化,降低草莓的腐烂率;壳聚糖处理的草莓样品组的呼吸强度分别由32.65,33.59,32.31 m L·(kg·h)-1升至69.98,62.42,64.63 m L·(kg·h)-1,而对照组的呼吸强度升至83.98m L·(kg·h)-1,其中壳聚糖1%-柠檬酸4%涂膜液的保鲜效果最好。展开更多
[Objective] The aim of this study was to screen Saccharomyces for glutathione over-production. [Method] Ethionine-resistant mutants were obtained through UV mutagenesis and rational screening. [Result] A high GSH-prod...[Objective] The aim of this study was to screen Saccharomyces for glutathione over-production. [Method] Ethionine-resistant mutants were obtained through UV mutagenesis and rational screening. [Result] A high GSH-producing strain HSJB1 was isolated from soil, and the biomass for this strain by flask shaking fermentation was 3.87 g/L while the GSH yield was 91.87 mg/L. According to the morphological, physiological and biochemical characteristics of cells, this strain was primarily identified as Saccharomyces cerevisiae. An ethionine-resistant mutant YBS77 was obtained through UV mutagenesis of the original strain HSJB1, and the biomass for this strain by flask shaking fermentation was 7.60 g dry cell weight/L while the GSH yield was 211.96 mg/L. [Conclusion] The biomass of the mutant obtained by breeding is increased by 96.38% than that of the original strain, and the GSH yield of the mutant obtained by breeding is increased by 130.72% than that from the original strain, which indicates that the breeding method is feasible.展开更多
文摘将草莓分别进行壳聚糖1%-柠檬酸2%、壳聚糖1%-柠檬酸3%、壳聚糖1%-柠檬酸4%、空白对照4种方式涂膜处理,于4℃下贮藏5 d,对其采后生理指标(颜色、硬度、腐烂率、可溶性固形物、呼吸强度、过氧化物酶和多酚氧化酶活性等)进行测定。结果表明:壳聚糖涂膜能减缓草莓贮藏过程中的褐变程度,延缓草莓果肉的软化,降低草莓的腐烂率;壳聚糖处理的草莓样品组的呼吸强度分别由32.65,33.59,32.31 m L·(kg·h)-1升至69.98,62.42,64.63 m L·(kg·h)-1,而对照组的呼吸强度升至83.98m L·(kg·h)-1,其中壳聚糖1%-柠檬酸4%涂膜液的保鲜效果最好。
基金Supported by Scientific Research Project of Liaoning Educational Department(20060154)Initial Funds for Doctors in Dalian Nationalities University(20066206)~~
文摘[Objective] The aim of this study was to screen Saccharomyces for glutathione over-production. [Method] Ethionine-resistant mutants were obtained through UV mutagenesis and rational screening. [Result] A high GSH-producing strain HSJB1 was isolated from soil, and the biomass for this strain by flask shaking fermentation was 3.87 g/L while the GSH yield was 91.87 mg/L. According to the morphological, physiological and biochemical characteristics of cells, this strain was primarily identified as Saccharomyces cerevisiae. An ethionine-resistant mutant YBS77 was obtained through UV mutagenesis of the original strain HSJB1, and the biomass for this strain by flask shaking fermentation was 7.60 g dry cell weight/L while the GSH yield was 211.96 mg/L. [Conclusion] The biomass of the mutant obtained by breeding is increased by 96.38% than that of the original strain, and the GSH yield of the mutant obtained by breeding is increased by 130.72% than that from the original strain, which indicates that the breeding method is feasible.
