大口鲇(Silurus meridionalis Chen),原名南方大口鲇,俗称河鲇,大河鲇,主产于我国长江流域的大江河中,是一种以鱼为食的大型经济鱼类,常见个体重约2～5公斤,最大个体可达40公斤以上。它与分布很广的那种小个子鲇(俗称土鲇)是同属不同种...大口鲇(Silurus meridionalis Chen),原名南方大口鲇,俗称河鲇,大河鲇,主产于我国长江流域的大江河中,是一种以鱼为食的大型经济鱼类,常见个体重约2～5公斤,最大个体可达40公斤以上。它与分布很广的那种小个子鲇(俗称土鲇)是同属不同种。大口鲇肉质细嫩、味道鲜美。我所从1985年起即开始对其进行了移养驯化和池塘人工养殖试验。展开更多
【目的】查明引起达氏鲟(Acipenser dabryanus)发病死亡的病原菌及其耐药性,为该病的临床诊断和科学防治提供参考依据。【方法】以常规方法对患病达氏鲟的鳃、心脏、肝脏和肠道等组织进行病原菌分离纯化,采用形态学观察、生理生化特性...【目的】查明引起达氏鲟(Acipenser dabryanus)发病死亡的病原菌及其耐药性,为该病的临床诊断和科学防治提供参考依据。【方法】以常规方法对患病达氏鲟的鳃、心脏、肝脏和肠道等组织进行病原菌分离纯化,采用形态学观察、生理生化特性鉴定与16S r RNA序列分析相结合的方法进行鉴定,通过人工感染试验明确病原菌的致病性及其病理组织学特征,并以K-B纸片扩散法进行药敏试验。【结果】从患病达氏鲟的肝脏组织中分离获得一株优势菌株(AD-AV201606),经形态学观察、生理生化特性鉴定及16S r RNA序列分析,最终鉴定为维氏气单胞菌(Aeromonas veronii)。以维氏气单胞菌人工感染达氏鲟,可复制出与自然发病相同的临床症状,感染引起的死亡率为50%~80%,且病鱼集中在感染后1~3 d死亡。药敏试验结果表明,达氏鲟维氏气单胞菌对庆大霉素、链霉素、左氧氟沙星、四环素、环丙沙星、阿奇霉素和头孢呋辛等7种抗生素敏感,对青霉素和利福平已产生耐药性。维氏气单胞菌感染对达氏鲟造成的组织病理变化主要集中在肝脏、肠道及鳃组织。【结论】维氏气单胞菌对达氏鲟有较强的致病性,通过引起肝脏、肠道及鳃等器官组织的病理变化而造成机体损伤甚至死亡,生产上可选用庆大霉素、链霉素和四环素进行防治。展开更多
Hepatic cells apoptosis induced by olaquindox in common carp, Cyprinus carpio, were studied. The test was conducted for 30 days, with dose of 200mg·kg-1 feed. Terminal deoxynucleotidy transferase mediated dUTP-Bi...Hepatic cells apoptosis induced by olaquindox in common carp, Cyprinus carpio, were studied. The test was conducted for 30 days, with dose of 200mg·kg-1 feed. Terminal deoxynucleotidy transferase mediated dUTP-Biotin nick-end labeling （TUNEL）, electronic microscopic and flow cytometry were performed to analyze apoptosis. The result showed that hepatic cells apoptosis were indentified under light microscope with TUNEL staining; chromatin condensation, chromosal clumping and margination, and apoptotic bodies were observed under electronic microscope; and sub-G1 PEAK （AP peak） in hepatic cells could be detected by flow cytometric method. The apoptotic rate measured by flow cytomtry increased with dye-poison time, on the 7th,14th,20th,25th and 30th day, the apoptotic rate was 2.05%, 6.25%, 9.49%, 13.31% and 17.44% respectively.展开更多
文摘大口鲇(Silurus meridionalis Chen),原名南方大口鲇,俗称河鲇,大河鲇,主产于我国长江流域的大江河中,是一种以鱼为食的大型经济鱼类,常见个体重约2～5公斤,最大个体可达40公斤以上。它与分布很广的那种小个子鲇(俗称土鲇)是同属不同种。大口鲇肉质细嫩、味道鲜美。我所从1985年起即开始对其进行了移养驯化和池塘人工养殖试验。
文摘【目的】查明引起达氏鲟(Acipenser dabryanus)发病死亡的病原菌及其耐药性,为该病的临床诊断和科学防治提供参考依据。【方法】以常规方法对患病达氏鲟的鳃、心脏、肝脏和肠道等组织进行病原菌分离纯化,采用形态学观察、生理生化特性鉴定与16S r RNA序列分析相结合的方法进行鉴定,通过人工感染试验明确病原菌的致病性及其病理组织学特征,并以K-B纸片扩散法进行药敏试验。【结果】从患病达氏鲟的肝脏组织中分离获得一株优势菌株(AD-AV201606),经形态学观察、生理生化特性鉴定及16S r RNA序列分析,最终鉴定为维氏气单胞菌(Aeromonas veronii)。以维氏气单胞菌人工感染达氏鲟,可复制出与自然发病相同的临床症状,感染引起的死亡率为50%~80%,且病鱼集中在感染后1~3 d死亡。药敏试验结果表明,达氏鲟维氏气单胞菌对庆大霉素、链霉素、左氧氟沙星、四环素、环丙沙星、阿奇霉素和头孢呋辛等7种抗生素敏感,对青霉素和利福平已产生耐药性。维氏气单胞菌感染对达氏鲟造成的组织病理变化主要集中在肝脏、肠道及鳃组织。【结论】维氏气单胞菌对达氏鲟有较强的致病性,通过引起肝脏、肠道及鳃等器官组织的病理变化而造成机体损伤甚至死亡,生产上可选用庆大霉素、链霉素和四环素进行防治。
文摘Hepatic cells apoptosis induced by olaquindox in common carp, Cyprinus carpio, were studied. The test was conducted for 30 days, with dose of 200mg·kg-1 feed. Terminal deoxynucleotidy transferase mediated dUTP-Biotin nick-end labeling （TUNEL）, electronic microscopic and flow cytometry were performed to analyze apoptosis. The result showed that hepatic cells apoptosis were indentified under light microscope with TUNEL staining; chromatin condensation, chromosal clumping and margination, and apoptotic bodies were observed under electronic microscope; and sub-G1 PEAK （AP peak） in hepatic cells could be detected by flow cytometric method. The apoptotic rate measured by flow cytomtry increased with dye-poison time, on the 7th,14th,20th,25th and 30th day, the apoptotic rate was 2.05%, 6.25%, 9.49%, 13.31% and 17.44% respectively.
