Taura syndrome virus(TSV) is one of the important pathogenic agents of Penaeus vannami,which causes prawn infection with serious disease in China in recent years.245 samples collected from 26 farms of Zhangzhou,Xiamen...Taura syndrome virus(TSV) is one of the important pathogenic agents of Penaeus vannami,which causes prawn infection with serious disease in China in recent years.245 samples collected from 26 farms of Zhangzhou,Xiamen,Shenzhen,Yangjiang,Ningbo and Guangzhou.were detected for TSV with RT-PCR,the positive rates were 100%,33.3%,40.7%,50% and 40%,respectively.Southern blotting and nucleotide sequencing were performed to confirm the specificity of the amplified RT-PCR products.The homology of 231bp nucleotide sequences reached 98.3%-100% when analysed and compared with the GenBank using the BLAST program.Phylogenetic analyses clustered the TSV isolates into two groups: one contained USA and Chinese Taiwan isolates;the other contained Vietnam isolate(YN1) and 21 isolates of China's Mainland.The 21 China isolates clustered into one branch,while the YN1 distributed in another branch.5 strains from Guangzhou and 2 strains from Shenzhen constituted one subcluster of the China branch.展开更多
Invasion of Aeromonas hydrophila strain Ah J-1 isolated from diseased fish to cultured HEp-2cells monolayer was evaluated by the recovery of gentamincin-resistant (Gm r)bacteria from Triton X-100 cell lysates. The inv...Invasion of Aeromonas hydrophila strain Ah J-1 isolated from diseased fish to cultured HEp-2cells monolayer was evaluated by the recovery of gentamincin-resistant (Gm r)bacteria from Triton X-100 cell lysates. The invasive efficiency (IE) could reach to 0.2% at 37℃. In addition, potential signal transduction pathways that precede bacterial internalization were studied by using signal transduction inhibitors. Bacterial internalization of Ah J-1 involved microfilaments and protein tyrosine kinase since cytochalasin D (an inhibitor of microfilament polymerization) and genistein (an inhibitor of protein tyrosine kinase) prevented internalization. Staurosporine(a protein kinase C inhibitor) and Sodium orthovanadate (a protein tyrosine phosphatase inhibitor) accelerated internalization of Ah J-1 into HEp-2 cells.展开更多
文摘Taura syndrome virus(TSV) is one of the important pathogenic agents of Penaeus vannami,which causes prawn infection with serious disease in China in recent years.245 samples collected from 26 farms of Zhangzhou,Xiamen,Shenzhen,Yangjiang,Ningbo and Guangzhou.were detected for TSV with RT-PCR,the positive rates were 100%,33.3%,40.7%,50% and 40%,respectively.Southern blotting and nucleotide sequencing were performed to confirm the specificity of the amplified RT-PCR products.The homology of 231bp nucleotide sequences reached 98.3%-100% when analysed and compared with the GenBank using the BLAST program.Phylogenetic analyses clustered the TSV isolates into two groups: one contained USA and Chinese Taiwan isolates;the other contained Vietnam isolate(YN1) and 21 isolates of China's Mainland.The 21 China isolates clustered into one branch,while the YN1 distributed in another branch.5 strains from Guangzhou and 2 strains from Shenzhen constituted one subcluster of the China branch.
文摘Invasion of Aeromonas hydrophila strain Ah J-1 isolated from diseased fish to cultured HEp-2cells monolayer was evaluated by the recovery of gentamincin-resistant (Gm r)bacteria from Triton X-100 cell lysates. The invasive efficiency (IE) could reach to 0.2% at 37℃. In addition, potential signal transduction pathways that precede bacterial internalization were studied by using signal transduction inhibitors. Bacterial internalization of Ah J-1 involved microfilaments and protein tyrosine kinase since cytochalasin D (an inhibitor of microfilament polymerization) and genistein (an inhibitor of protein tyrosine kinase) prevented internalization. Staurosporine(a protein kinase C inhibitor) and Sodium orthovanadate (a protein tyrosine phosphatase inhibitor) accelerated internalization of Ah J-1 into HEp-2 cells.
