The present paper covers a novel technology for the concentration of trace amounts of target oligonucleotide from the solution. This technique is based on a super-paramagnetic DNA nano-enricher constructed with a sing...The present paper covers a novel technology for the concentration of trace amounts of target oligonucleotide from the solution. This technique is based on a super-paramagnetic DNA nano-enricher constructed with a single strand DNA probe immobilized onto the surface of the super-paramagnetic nanoparticles prepared by using the water-in-oil microemulsion technique, employing silica as the shell and iron oxide as the core of the super-paramagnetic nanoparticles. The silica coated magnetic nanoparticles are (40±4) nm in size. And the magnetic nanoparticle is super-paramagnetic. Biotin labeled ssDNA(Biotin-5-(A)10-GAT-TCA-CGA-GGC-CCT-AGT-CG-3) was immobilized on the surface of silica coated magnetic nanoparticles. The complementary ssDNA could be enriched effectively and the characteristics of the enriched ssDNA have not changed, which will provide a novel technique and measurement for gene transfection, mutation detection, gene diagnosis, gene therapy and so on.展开更多
An attempt was made to combine molecular imprinting technique and the electropolymerization of self-assembled o-amino thiophenol（o-AT） to prepare imprinted film using cinchonine as the model template analyte. The pr...An attempt was made to combine molecular imprinting technique and the electropolymerization of self-assembled o-amino thiophenol（o-AT） to prepare imprinted film using cinchonine as the model template analyte. The procedure of forming recognition cavity and the effect of the ratio of monomers to templates on the imprinted film, together with sensitivity and selectivity to cinchonine on the imprinted electrode, were demonstrated. An indirect and rapid detection was carried out using potassium ferricyande as a probe. Stable response is achieved within 4 min, covering a linear range (5.0×10-6\)4.0×10-5 mol/L. From the results, the main driving force for recognition is supposed to be hydrophobic interaction, complementary cavity effect and phenyl group for π-π interactions with the enaphthyl residue.展开更多
文摘The present paper covers a novel technology for the concentration of trace amounts of target oligonucleotide from the solution. This technique is based on a super-paramagnetic DNA nano-enricher constructed with a single strand DNA probe immobilized onto the surface of the super-paramagnetic nanoparticles prepared by using the water-in-oil microemulsion technique, employing silica as the shell and iron oxide as the core of the super-paramagnetic nanoparticles. The silica coated magnetic nanoparticles are (40±4) nm in size. And the magnetic nanoparticle is super-paramagnetic. Biotin labeled ssDNA(Biotin-5-(A)10-GAT-TCA-CGA-GGC-CCT-AGT-CG-3) was immobilized on the surface of silica coated magnetic nanoparticles. The complementary ssDNA could be enriched effectively and the characteristics of the enriched ssDNA have not changed, which will provide a novel technique and measurement for gene transfection, mutation detection, gene diagnosis, gene therapy and so on.
文摘An attempt was made to combine molecular imprinting technique and the electropolymerization of self-assembled o-amino thiophenol（o-AT） to prepare imprinted film using cinchonine as the model template analyte. The procedure of forming recognition cavity and the effect of the ratio of monomers to templates on the imprinted film, together with sensitivity and selectivity to cinchonine on the imprinted electrode, were demonstrated. An indirect and rapid detection was carried out using potassium ferricyande as a probe. Stable response is achieved within 4 min, covering a linear range (5.0×10-6\)4.0×10-5 mol/L. From the results, the main driving force for recognition is supposed to be hydrophobic interaction, complementary cavity effect and phenyl group for π-π interactions with the enaphthyl residue.
