将白藜芦醇添加至丹阳黄酒中,形成含有质量浓度为0.1 mg/m L白藜芦醇的新型丹阳黄酒,以此为研究对象,对其清除DPPH、羟基、ABTS自由基和总还原能力进行测定,来评价白藜芦醇丹阳黄酒产品的抗氧化活性及稳定性。结果表明,0.1 mg/m L的白...将白藜芦醇添加至丹阳黄酒中,形成含有质量浓度为0.1 mg/m L白藜芦醇的新型丹阳黄酒,以此为研究对象,对其清除DPPH、羟基、ABTS自由基和总还原能力进行测定,来评价白藜芦醇丹阳黄酒产品的抗氧化活性及稳定性。结果表明,0.1 mg/m L的白藜芦醇丹阳黄酒对DPPH、羟基、ABTS自由基的半抑制浓度(IC_(50)值)依次为0.40 m L/m L、0.80 m L/m L、0.51 m L/m L,普通丹阳黄酒IC_(50)值依次0.70 m L/m L、0.81 m L/m L、0.80 m L/m L,新型丹阳黄酒的总还原力明显高于普通丹阳黄酒。综合比较,含白藜芦醇丹阳黄酒抗氧化能力高于普通丹阳黄酒。同时,新型丹阳黄酒在常温下存放前3 d的白藜芦醇含量快速下降,之后趋于稳定。展开更多
孵化酶是后生动物卵孵化过程中起关键作用的一类蛋白酶。基于生物信息学和RACE方法,从柞蚕胚胎中克隆了一个998 bp的孵化酶样基因全长cDNA,命名为ApHEL(GenBank登录号:JN205047)。ApHEL由15 bp 5'-UTR,98 bp 3'-UTR和885 bp OR...孵化酶是后生动物卵孵化过程中起关键作用的一类蛋白酶。基于生物信息学和RACE方法,从柞蚕胚胎中克隆了一个998 bp的孵化酶样基因全长cDNA,命名为ApHEL(GenBank登录号:JN205047)。ApHEL由15 bp 5'-UTR,98 bp 3'-UTR和885 bp ORF组成。ORF编码294个氨基酸残基,预测蛋白分子质量为33.51 kD,等电点为5.50。在ApHEL N-端存在16个氨基酸的信号肽。ApHEL蛋白酶功能区中含有锌结合序列(HEWMHILGFLHMQSTHNR)和Met-转角基序(YDYVSCLHY)等孵化酶保守功能区域。ApHEL与其他物种孵化酶氨基酸序列的相似度为30.0%～85.0%。基于孵化酶氨基酸序列的进化分析表明,柞蚕、家蚕、库蚊和果蝇聚为一类,亲缘关系较近。ApHEL在早期胚胎中没有转录,至催青第6天开始转录并维持较低水平,在催青第9天剧增至最高值直至孵化,显示ApHEL在柞蚕卵孵化过程中起重要作用。ApHEL在5龄期幼虫的中肠组织中特异性表达,暗示ApHEL可能还有其他生物学功能。展开更多
[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extru...[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extruding and centrifugal method, and then the semen was injected into other virgin moths with trypsinase. [ Result] A high-effective collection technology of spermatids from silkworm was established successfully, 50 μl semen could be collected by only one person in each hour. The survival rate of spermatids was over 80% in vito after collected from bursa copulatrix, while the obtained semen was quite pure and the average fertilization rate of silkworm was 76,5%. [ Conclusion] The establishment of high-effective semen extraction technique of silkworm provides the technical basis for studies on other related techniques for silkworm sperm.展开更多
Mulberry yellow dwarf(MYD)disease is an quarantine disease and the causal agent is a phytoplasma.Two pairs of published universal primer,P1/P7 and Rm16F2/Rm16R1,based on the 16S-23S rDNA sequence of phytoplasma and to...Mulberry yellow dwarf(MYD)disease is an quarantine disease and the causal agent is a phytoplasma.Two pairs of published universal primer,P1/P7 and Rm16F2/Rm16R1,based on the 16S-23S rDNA sequence of phytoplasma and total DNA extracted from infected mulberry tissues were employed for PCR and nested-PCR detection.The results revealed that a phytoplasma-specific 1 830 bp fragment with a G+C content of 46.01% was sequenced(GenBank accession No.GQ249410).The sequence shared 99.7% and 99.8% identity with aster yellows,the representatiive phytoplasma in 16SrI group,and mulberry dwarf phytoplasma classified into subgroup B in 16SrI group and named as the MYD phytoplasma strain Anhui(MYD-Anh).A phylogenetic tree based on 16S rDNA sequences was constructed and showed that MYD-Anh was clustered into 16SrI group.Identity of 16S rDNA sequence between MYD-Anh and mulberry yellow dwarf phytoplasma strain Zhenjiang(MD-zj) was nearly 100%,and they might belong to the same strain.Nested-PCR was used to detect the pathogenic phytoplasma from the differential tissues of mulberry infected with MYD-Anh.The results showed that a phytoplasma-specific 1.4 kb fragment was amplified with total DNA extracted from bark and vein.Nested-PCR was more sensitive than PCR for detecting MYD phytoplasma.展开更多
文摘将白藜芦醇添加至丹阳黄酒中,形成含有质量浓度为0.1 mg/m L白藜芦醇的新型丹阳黄酒,以此为研究对象,对其清除DPPH、羟基、ABTS自由基和总还原能力进行测定,来评价白藜芦醇丹阳黄酒产品的抗氧化活性及稳定性。结果表明,0.1 mg/m L的白藜芦醇丹阳黄酒对DPPH、羟基、ABTS自由基的半抑制浓度(IC_(50)值)依次为0.40 m L/m L、0.80 m L/m L、0.51 m L/m L,普通丹阳黄酒IC_(50)值依次0.70 m L/m L、0.81 m L/m L、0.80 m L/m L,新型丹阳黄酒的总还原力明显高于普通丹阳黄酒。综合比较,含白藜芦醇丹阳黄酒抗氧化能力高于普通丹阳黄酒。同时,新型丹阳黄酒在常温下存放前3 d的白藜芦醇含量快速下降,之后趋于稳定。
文摘孵化酶是后生动物卵孵化过程中起关键作用的一类蛋白酶。基于生物信息学和RACE方法,从柞蚕胚胎中克隆了一个998 bp的孵化酶样基因全长cDNA,命名为ApHEL(GenBank登录号:JN205047)。ApHEL由15 bp 5'-UTR,98 bp 3'-UTR和885 bp ORF组成。ORF编码294个氨基酸残基,预测蛋白分子质量为33.51 kD,等电点为5.50。在ApHEL N-端存在16个氨基酸的信号肽。ApHEL蛋白酶功能区中含有锌结合序列(HEWMHILGFLHMQSTHNR)和Met-转角基序(YDYVSCLHY)等孵化酶保守功能区域。ApHEL与其他物种孵化酶氨基酸序列的相似度为30.0%～85.0%。基于孵化酶氨基酸序列的进化分析表明,柞蚕、家蚕、库蚊和果蝇聚为一类,亲缘关系较近。ApHEL在早期胚胎中没有转录,至催青第6天开始转录并维持较低水平,在催青第9天剧增至最高值直至孵化,显示ApHEL在柞蚕卵孵化过程中起重要作用。ApHEL在5龄期幼虫的中肠组织中特异性表达,暗示ApHEL可能还有其他生物学功能。
文摘[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extruding and centrifugal method, and then the semen was injected into other virgin moths with trypsinase. [ Result] A high-effective collection technology of spermatids from silkworm was established successfully, 50 μl semen could be collected by only one person in each hour. The survival rate of spermatids was over 80% in vito after collected from bursa copulatrix, while the obtained semen was quite pure and the average fertilization rate of silkworm was 76,5%. [ Conclusion] The establishment of high-effective semen extraction technique of silkworm provides the technical basis for studies on other related techniques for silkworm sperm.
文摘Mulberry yellow dwarf(MYD)disease is an quarantine disease and the causal agent is a phytoplasma.Two pairs of published universal primer,P1/P7 and Rm16F2/Rm16R1,based on the 16S-23S rDNA sequence of phytoplasma and total DNA extracted from infected mulberry tissues were employed for PCR and nested-PCR detection.The results revealed that a phytoplasma-specific 1 830 bp fragment with a G+C content of 46.01% was sequenced(GenBank accession No.GQ249410).The sequence shared 99.7% and 99.8% identity with aster yellows,the representatiive phytoplasma in 16SrI group,and mulberry dwarf phytoplasma classified into subgroup B in 16SrI group and named as the MYD phytoplasma strain Anhui(MYD-Anh).A phylogenetic tree based on 16S rDNA sequences was constructed and showed that MYD-Anh was clustered into 16SrI group.Identity of 16S rDNA sequence between MYD-Anh and mulberry yellow dwarf phytoplasma strain Zhenjiang(MD-zj) was nearly 100%,and they might belong to the same strain.Nested-PCR was used to detect the pathogenic phytoplasma from the differential tissues of mulberry infected with MYD-Anh.The results showed that a phytoplasma-specific 1.4 kb fragment was amplified with total DNA extracted from bark and vein.Nested-PCR was more sensitive than PCR for detecting MYD phytoplasma.
