Objective： To explore susceptibility genes associated with yang-deficiency constitution using single nucleotide polymorphism （SNP） genotyping. Methods： Based on an epidemiological survey, 30 volunteers with yang-d...Objective： To explore susceptibility genes associated with yang-deficiency constitution using single nucleotide polymorphism （SNP） genotyping. Methods： Based on an epidemiological survey, 30 volunteers with yang-deficiency constitution and 30 volunteers with a balanced constitution were included according to the Classification and Determination Standards of Constitutions in Traditional Chinese Medicine. Peripheral blood was collected and DNA was extracted from white blood cells. A genome-wide association study （GWAS） was conducted by SNP 6.0 genotyping at the Beijing Capital Bio Corporation Ltd. A minimum association P-value （Fisher＇s exact value） of less than 104 in the allele, genotype, dominant, and recessive models served as the standard for significant association of SNP with yang-deficiency constitution. Results： Among the four genetic models, a total of 42 SNPs were significantly associated with yang-deficiency constitution （Fisher＇s exact P-values 〈10^4）. These SNPs were adjacent to more than 20 genes, including RGS6, mGluR5, GAPDHL19, and II〈ZF1. Conclusions： Yang-deficiency constitution exhibits the characteristics of polygenic inheritance. This pilot study suggests that the polymorphisms in RGS6, mGluR5, GAPDHL19, and IKZF1 are associated with changes in cyclic adenosine monophosphate and cyclic guanosine monophosphate levels, memory, metabolic energy status, and immune function, respectively in people with yang-deficiency constitution.展开更多
The Yang-deficiency constitution has been a hot topic of interest in recent years.Important research progress has been made in quantitative diagnostic criteria and auxiliary identification technologies,modern biologic...The Yang-deficiency constitution has been a hot topic of interest in recent years.Important research progress has been made in quantitative diagnostic criteria and auxiliary identification technologies,modern biological interpretations of the constitution characteristics,the association between the Yang-deficiency constitution and diseases,as well as the mechanisms of regulating the constitution.Yet,there are still some deficiencies and limitations.Herein,we systematically reviewed the research progress on the Yang-deficiency constitution by searching articles published in China National Knowledge Infrastructure Database,China Science and Technology Journal Database,Wanfang Database,PubMed,and Embase.The future development direction of the Yangdeficiency constitution should focus on developing objective constitution identification techniques,establishing animal models of Yang-deficiency constitution,conducting constitutional and diseaserelated research with a high level of evidence,combined with research on the full chain of the nine constitutions so as to obtain more scientific and objective conclusions.展开更多
Objective:To study the differences between the serum metabolites in patients with adenomatous polyps of the colon and yang-deficiency constitution and those without colon polyps and with balanced constitution,and look...Objective:To study the differences between the serum metabolites in patients with adenomatous polyps of the colon and yang-deficiency constitution and those without colon polyps and with balanced constitution,and look for biomarkers that can be used to distinguish between the two groups.Methods:General patient information was gathered,and Chinese medicine constitutions were collected in 940 patients who underwent electronic colonoscopy.A total of 119 patients with adenomatous polyps of the colon and yang-deficiency constitution were included in the experimental group,and 150 patients without colon polyps and with balanced constitution were included in the control group.Metabolomics analysis was performed on the fasting venous blood obtained from each patient in both groups.Principal component analysis and orthogonal partial least squares discriminant analysis were performed on the detection results,potential biomarkers were screened,metabolic pathway changes were determined,and the metabolic processes involved were discussed.Results:A total of 59 differential biomarkers between the experimental group and the control group were identified.The differential metabolites were found mainly in the glycerophospholipid metabolism pathway,and the bile acid 3-oxo-4,6-choladienoic acid was the biomarker that distinguished the experimental group from the control group.Conclusions:With the help of metabolomics analysis,the differential metabolites in patients with adenomatous polyps of the colon and yang-deficiency constitution and those in patients without colon polyps and with balanced constitution could be identified.The biomarker 3-oxo-4,6-choladienoic acid may have potential diagnostic value in patients with adenomatous polyp of the colon and yang-deficiency constitution.(Trial Registration No.NCT02986308)展开更多
基金Supported by the National Natural Science Foundation of China(No.81072715)
文摘Objective： To explore susceptibility genes associated with yang-deficiency constitution using single nucleotide polymorphism （SNP） genotyping. Methods： Based on an epidemiological survey, 30 volunteers with yang-deficiency constitution and 30 volunteers with a balanced constitution were included according to the Classification and Determination Standards of Constitutions in Traditional Chinese Medicine. Peripheral blood was collected and DNA was extracted from white blood cells. A genome-wide association study （GWAS） was conducted by SNP 6.0 genotyping at the Beijing Capital Bio Corporation Ltd. A minimum association P-value （Fisher＇s exact value） of less than 104 in the allele, genotype, dominant, and recessive models served as the standard for significant association of SNP with yang-deficiency constitution. Results： Among the four genetic models, a total of 42 SNPs were significantly associated with yang-deficiency constitution （Fisher＇s exact P-values 〈10^4）. These SNPs were adjacent to more than 20 genes, including RGS6, mGluR5, GAPDHL19, and II〈ZF1. Conclusions： Yang-deficiency constitution exhibits the characteristics of polygenic inheritance. This pilot study suggests that the polymorphisms in RGS6, mGluR5, GAPDHL19, and IKZF1 are associated with changes in cyclic adenosine monophosphate and cyclic guanosine monophosphate levels, memory, metabolic energy status, and immune function, respectively in people with yang-deficiency constitution.
基金Supported by the National Natural Science Foundation of China(Mechanism of Regulating the Microbiota-Gut-Brain Axis in Hypodynamic Symptoms of Depression Easily Occurred in YangDeficiency Constitution and Improved by Adjusting the Constitution,No.82074291the Intestinal Microecology Mechanism of Gui Fu Di Huang Pills in Regulating the Energy Metabolism and NervousEndocrine Network of Yang Deficiency Constitution People,No.81774161)the Strategic Research and Consulting Project of Chinese Academy of Engineering:Strategic Research on“Preventive Treatment of Disease”by Traditional Chinese Medicine and Construction of Integrated TCM and Western Medicine Disease Prevention and Control System(No.2021-XBZD-1)。
文摘The Yang-deficiency constitution has been a hot topic of interest in recent years.Important research progress has been made in quantitative diagnostic criteria and auxiliary identification technologies,modern biological interpretations of the constitution characteristics,the association between the Yang-deficiency constitution and diseases,as well as the mechanisms of regulating the constitution.Yet,there are still some deficiencies and limitations.Herein,we systematically reviewed the research progress on the Yang-deficiency constitution by searching articles published in China National Knowledge Infrastructure Database,China Science and Technology Journal Database,Wanfang Database,PubMed,and Embase.The future development direction of the Yangdeficiency constitution should focus on developing objective constitution identification techniques,establishing animal models of Yang-deficiency constitution,conducting constitutional and diseaserelated research with a high level of evidence,combined with research on the full chain of the nine constitutions so as to obtain more scientific and objective conclusions.
文摘Objective:To study the differences between the serum metabolites in patients with adenomatous polyps of the colon and yang-deficiency constitution and those without colon polyps and with balanced constitution,and look for biomarkers that can be used to distinguish between the two groups.Methods:General patient information was gathered,and Chinese medicine constitutions were collected in 940 patients who underwent electronic colonoscopy.A total of 119 patients with adenomatous polyps of the colon and yang-deficiency constitution were included in the experimental group,and 150 patients without colon polyps and with balanced constitution were included in the control group.Metabolomics analysis was performed on the fasting venous blood obtained from each patient in both groups.Principal component analysis and orthogonal partial least squares discriminant analysis were performed on the detection results,potential biomarkers were screened,metabolic pathway changes were determined,and the metabolic processes involved were discussed.Results:A total of 59 differential biomarkers between the experimental group and the control group were identified.The differential metabolites were found mainly in the glycerophospholipid metabolism pathway,and the bile acid 3-oxo-4,6-choladienoic acid was the biomarker that distinguished the experimental group from the control group.Conclusions:With the help of metabolomics analysis,the differential metabolites in patients with adenomatous polyps of the colon and yang-deficiency constitution and those in patients without colon polyps and with balanced constitution could be identified.The biomarker 3-oxo-4,6-choladienoic acid may have potential diagnostic value in patients with adenomatous polyp of the colon and yang-deficiency constitution.(Trial Registration No.NCT02986308)
