Current research on bone marrow stem cell transplantation and autologous or xenogenic nerve transplantation for peripheral nerve regeneration has mainly focused on the repair of peripher-al nerve defects in rodents. I...Current research on bone marrow stem cell transplantation and autologous or xenogenic nerve transplantation for peripheral nerve regeneration has mainly focused on the repair of peripher-al nerve defects in rodents. In this study, we established a standardized experimental model of radial nerve defects in primates and evaluated the effect of repair on peripheral nerve injury. We repaired 2.5-cm lesions in the radial nerve of rhesus monkeys by transplantation of autografts, acellular allografts, or acellular allografts seeded with autologous bone marrow stem cells. Five months after surgery, regenerated nerve tissue was assessed for function, electrophysiology, and histomorphometry. Postoperative functional recovery was evaluated by the wrist-extension test. Compared with the simple autografts, the acellular allografts and allografts seeded with bone marrow stem cells facilitated remarkable recovery of the wrist-extension functions in the rhesus monkeys. This functional improvement was coupled with radial nerve distal axon growth, a higher percentage of neuron survival, increased nerve fiber density and diameter, increased myelin sheath thickness, and increased nerve conduction velocities and peak amplitudes of compound motor action potentials. Furthermore, the quality of nerve regeneration in the bone marrow stem cells-laden allografts group was comparable to that achieved with autografts. The wrist-extension test is a simple behavioral method for objective quantification of peripheral nerve regeneration.展开更多
目的:观察交叉迁移联合肌电生物反馈干预对亚急性期脑卒中患者腕背伸功能的影响。方法:将45例亚急性期脑卒中患者随机分为交叉迁移组、肌电生物反馈组、联合组,每组各15例。3组患者均接受常规康复训练,交叉迁移组在此基础上进行交叉迁...目的:观察交叉迁移联合肌电生物反馈干预对亚急性期脑卒中患者腕背伸功能的影响。方法:将45例亚急性期脑卒中患者随机分为交叉迁移组、肌电生物反馈组、联合组,每组各15例。3组患者均接受常规康复训练,交叉迁移组在此基础上进行交叉迁移治疗,肌电生物反馈组在此基础上进行肌电生物反馈治疗,联合组在此基础上进行交叉迁移联合肌电生物反馈治疗。以上每组治疗需持续6周,并于治疗前、治疗后采用主动关节活动度(active joint range of motion,AROM)、徒手肌力评定(manual muscle test,MMT)、上肢Fugl-Meyer评分(FMA-UE)、改良Barthel指数(modified Barthel index,MBI)对患者腕背伸功能、上肢运动功能及日常生活活动能力进行评定。结果:治疗前,3组患者的FMA-UE评分、腕背伸AROM及肌力评分、MBI评分比较均无显著性差异(P>0.05)。治疗6周后,3组患者的FMA-UE评分、腕背伸AROM及肌力评分、MBI评分与治疗前相比均明显提高(P<0.05)。其中,联合组与交叉迁移组、肌电生物反馈组相比,除MBI评分比较无显著性差异(P>0.05)外,其他指标评分比较均有显著性差异(P<0.05),而交叉迁移组和肌电生物反馈组各项指标评分比较均无显著性差异(P>0.05)。结论:交叉迁移联合肌电生物反馈干预在改善亚急性期脑卒中患者腕背伸功能、上肢运动功能及日常生活活动能力方面优于单一的交叉迁移或肌电生物反馈疗法。展开更多
基金supported by the National High-Technology Research and Development Program of China(863 Program),No.2006AA02A130the National Natural Science Foundation of China,No.81372041,31070869,30700847
文摘Current research on bone marrow stem cell transplantation and autologous or xenogenic nerve transplantation for peripheral nerve regeneration has mainly focused on the repair of peripher-al nerve defects in rodents. In this study, we established a standardized experimental model of radial nerve defects in primates and evaluated the effect of repair on peripheral nerve injury. We repaired 2.5-cm lesions in the radial nerve of rhesus monkeys by transplantation of autografts, acellular allografts, or acellular allografts seeded with autologous bone marrow stem cells. Five months after surgery, regenerated nerve tissue was assessed for function, electrophysiology, and histomorphometry. Postoperative functional recovery was evaluated by the wrist-extension test. Compared with the simple autografts, the acellular allografts and allografts seeded with bone marrow stem cells facilitated remarkable recovery of the wrist-extension functions in the rhesus monkeys. This functional improvement was coupled with radial nerve distal axon growth, a higher percentage of neuron survival, increased nerve fiber density and diameter, increased myelin sheath thickness, and increased nerve conduction velocities and peak amplitudes of compound motor action potentials. Furthermore, the quality of nerve regeneration in the bone marrow stem cells-laden allografts group was comparable to that achieved with autografts. The wrist-extension test is a simple behavioral method for objective quantification of peripheral nerve regeneration.
文摘目的:观察交叉迁移联合肌电生物反馈干预对亚急性期脑卒中患者腕背伸功能的影响。方法:将45例亚急性期脑卒中患者随机分为交叉迁移组、肌电生物反馈组、联合组,每组各15例。3组患者均接受常规康复训练,交叉迁移组在此基础上进行交叉迁移治疗,肌电生物反馈组在此基础上进行肌电生物反馈治疗,联合组在此基础上进行交叉迁移联合肌电生物反馈治疗。以上每组治疗需持续6周,并于治疗前、治疗后采用主动关节活动度(active joint range of motion,AROM)、徒手肌力评定(manual muscle test,MMT)、上肢Fugl-Meyer评分(FMA-UE)、改良Barthel指数(modified Barthel index,MBI)对患者腕背伸功能、上肢运动功能及日常生活活动能力进行评定。结果:治疗前,3组患者的FMA-UE评分、腕背伸AROM及肌力评分、MBI评分比较均无显著性差异(P>0.05)。治疗6周后,3组患者的FMA-UE评分、腕背伸AROM及肌力评分、MBI评分与治疗前相比均明显提高(P<0.05)。其中,联合组与交叉迁移组、肌电生物反馈组相比,除MBI评分比较无显著性差异(P>0.05)外,其他指标评分比较均有显著性差异(P<0.05),而交叉迁移组和肌电生物反馈组各项指标评分比较均无显著性差异(P>0.05)。结论:交叉迁移联合肌电生物反馈干预在改善亚急性期脑卒中患者腕背伸功能、上肢运动功能及日常生活活动能力方面优于单一的交叉迁移或肌电生物反馈疗法。
