Chaste tree(Vitex negundo var.heterophylla)witches’-broom was a new disease found in Mountain Taishan and the neighboring region.Phytoplasmal cells could be easily observed in the phloem sieve elements of diseased pl...Chaste tree(Vitex negundo var.heterophylla)witches’-broom was a new disease found in Mountain Taishan and the neighboring region.Phytoplasmal cells could be easily observed in the phloem sieve elements of diseased plants under electron microscope.The 16S rRNA gene of phytoplasma associated with this disease(CTWB)was amplified with nested-PCR with the universal primer pairs R16mF2/R16mR1 and R16F2n/R16R2.An amplified fragment of ca.1.2 kb was sequenced and subjected to identity and phylogenetic analyses.Phytoplasma CTWB shared identities of more than 99.0% with members of Aster yellows group(16SrI)in 16S rRNA genes.It had the highest identity of 99.8% with phytoplasmas associated with Maize bushy stunt(subgroup 16SrI-B),and clustered into a common branch with phytoplasmas of the subgroup in the phylogenetic tree constructed with 16S rRNA genes.These results indicated that CTWB belonged to subgroup B of Candidatus Phytoplasma asteris(16SrI-B).展开更多
【目的】筛查北京地区枣园感染枣疯病植原体的叶蝉种类,确定枣疯病植原体潜在介体昆虫,为预防和控制枣疯病流行提供科学依据。【方法】通过扫网法和黄板诱集法,对昌平区流村镇流村、海淀区西北旺镇唐家岭村、朝阳区奥林匹克森林公园、...【目的】筛查北京地区枣园感染枣疯病植原体的叶蝉种类,确定枣疯病植原体潜在介体昆虫,为预防和控制枣疯病流行提供科学依据。【方法】通过扫网法和黄板诱集法,对昌平区流村镇流村、海淀区西北旺镇唐家岭村、朝阳区奥林匹克森林公园、通州区永于路北京观光南瓜园4个地点枣树上叶蝉种类及枣疯病植原体感染的叶蝉进行调查和分子检测分析。【结果】北京地区枣园枣树上有13种叶蝉发生,其中包括片突菱纹叶蝉( Hishimonus lamellatus Cai et Kuoh)、凹缘菱纹叶蝉( H. sellatus (Uhler))、小绿叶蝉( Empoasca spp.)、斑叶蝉( Erythroneura sp.)、横带叶蝉( Scaphoideus festivus Matsumura)、大青叶蝉( Cicadella viridis (Linnaeus))、新县长突叶蝉( Batracomorphus xinxianensis Cai et Shen)、红闪小叶蝉( Zygina sp.)、白边大叶蝉( Kolla paulula (Walker))、桃一点叶蝉( Singapora shinshana (Matsumura))、一点木叶蝉( Phlogotettix cyclops (Mulsant et Rey))、条沙叶蝉( Psammotettix striatus (Linnaeus))和窗耳叶蝉( Ledra auditura Walker)。通过对采集的叶蝉标本采用植原体通用引物进行PCR检测,片突菱纹叶蝉( H. lamellatus )、凹缘菱纹叶蝉( H. sellatus )、大青叶蝉( C. viridis )、白边大叶蝉( K. paulula ),均发现感染枣疯病植原体,感染率分别为1.4%、3.0%、7.4%、8.3%,其他叶蝉经检测未发现感染植原体。【结论】凹缘菱纹叶蝉( H. sellatus )、片突菱纹叶蝉( H. lamellatus )、大青叶蝉( C. viridis )、白边大叶蝉( K. paulula )可能为潜在的枣疯病植原体介体昆虫。展开更多
文摘Chaste tree(Vitex negundo var.heterophylla)witches’-broom was a new disease found in Mountain Taishan and the neighboring region.Phytoplasmal cells could be easily observed in the phloem sieve elements of diseased plants under electron microscope.The 16S rRNA gene of phytoplasma associated with this disease(CTWB)was amplified with nested-PCR with the universal primer pairs R16mF2/R16mR1 and R16F2n/R16R2.An amplified fragment of ca.1.2 kb was sequenced and subjected to identity and phylogenetic analyses.Phytoplasma CTWB shared identities of more than 99.0% with members of Aster yellows group(16SrI)in 16S rRNA genes.It had the highest identity of 99.8% with phytoplasmas associated with Maize bushy stunt(subgroup 16SrI-B),and clustered into a common branch with phytoplasmas of the subgroup in the phylogenetic tree constructed with 16S rRNA genes.These results indicated that CTWB belonged to subgroup B of Candidatus Phytoplasma asteris(16SrI-B).
文摘【目的】筛查北京地区枣园感染枣疯病植原体的叶蝉种类,确定枣疯病植原体潜在介体昆虫,为预防和控制枣疯病流行提供科学依据。【方法】通过扫网法和黄板诱集法,对昌平区流村镇流村、海淀区西北旺镇唐家岭村、朝阳区奥林匹克森林公园、通州区永于路北京观光南瓜园4个地点枣树上叶蝉种类及枣疯病植原体感染的叶蝉进行调查和分子检测分析。【结果】北京地区枣园枣树上有13种叶蝉发生,其中包括片突菱纹叶蝉( Hishimonus lamellatus Cai et Kuoh)、凹缘菱纹叶蝉( H. sellatus (Uhler))、小绿叶蝉( Empoasca spp.)、斑叶蝉( Erythroneura sp.)、横带叶蝉( Scaphoideus festivus Matsumura)、大青叶蝉( Cicadella viridis (Linnaeus))、新县长突叶蝉( Batracomorphus xinxianensis Cai et Shen)、红闪小叶蝉( Zygina sp.)、白边大叶蝉( Kolla paulula (Walker))、桃一点叶蝉( Singapora shinshana (Matsumura))、一点木叶蝉( Phlogotettix cyclops (Mulsant et Rey))、条沙叶蝉( Psammotettix striatus (Linnaeus))和窗耳叶蝉( Ledra auditura Walker)。通过对采集的叶蝉标本采用植原体通用引物进行PCR检测,片突菱纹叶蝉( H. lamellatus )、凹缘菱纹叶蝉( H. sellatus )、大青叶蝉( C. viridis )、白边大叶蝉( K. paulula ),均发现感染枣疯病植原体,感染率分别为1.4%、3.0%、7.4%、8.3%,其他叶蝉经检测未发现感染植原体。【结论】凹缘菱纹叶蝉( H. sellatus )、片突菱纹叶蝉( H. lamellatus )、大青叶蝉( C. viridis )、白边大叶蝉( K. paulula )可能为潜在的枣疯病植原体介体昆虫。
