High myopia has long been highly prevalent worldwide with a largely yet unexplained genetic contribution.To identify novel susceptibility genes for axial length(AL)in highly myopic eyes,a genome-wide association study...High myopia has long been highly prevalent worldwide with a largely yet unexplained genetic contribution.To identify novel susceptibility genes for axial length(AL)in highly myopic eyes,a genome-wide association study(GWAS)was performed using the genomic dataset of 350 deep whole-genome sequencing data from highly myopic patients.Top single nucleotide polymorphisms(SNPs)were functionally annotated.Immunofluorescence staining,quantitative polymerase chain reaction,and western blot were performed using neural retina of form-deprived myopic mice.Enrichment analyses were further performed.We identified the four top SNPs and found that ADAM Metallopeptidase With Thrombospondin Type 1 Motif 16(ADAMTS16)and Phosphatidylinositol Glycan Anchor Biosynthesis Class Z(PIGZ)had the potential of clinical signifi-cance.Animal experiments confirmed that PIGZ expression could be observed and showed higher expression level in form-deprived mice,especially in the ganglion cell layer.The messenger RNA(mRNA)levels of both ADAMTS16 and PIGZ were significantly higher in the neural retina of form-deprived eyes(p=0.005 and 0.007 respectively),and both proteins showed significantly upregulated expression in the neural retina of deprived eyes(p=0.004 and 0.042,respectively).Enrichment analysis revealed a significant role of cellular adhesion and signal transduction in AL,and also several AL-related pathways including circadian entrainment and inflammatory mediator regulation of transient receptor potential channels were proposed.In conclusion,the current study identified four novel SNPs associated with AL in highly myopic eyes and confirmed that the expression of ADAMTS16 and PIGZ was significantly upregulated in neural retina of deprived eyes.Enrichment analyses provided novel insight into the etiology of high myopia and opened avenues for future research interest.展开更多
This study views pedagogical translation as a means of developing language skills, especially oral communication. Contrary to the claim that translation focuses only on reading and writing, this study presents anecdot...This study views pedagogical translation as a means of developing language skills, especially oral communication. Contrary to the claim that translation focuses only on reading and writing, this study presents anecdotal evidence showing that a learner-centered approach to translation can help foreign language students improve their speaking skill through group work, problem-solving, and cooperative learning. To this end, the paper presents practical examples of lexical, structural, textual, and cultural issues that students discussed in their process-oriented Arabic-English-Arabic pedagogical translation courses at the university level. The students were divided into groups of three or four to work together at all stages of the translation process. They spent most of the class time comprehending the source text, translating it, and discussing each other＇s target versions. In the remaining class time, the whole class discussed a target text translated by any one of the groups. The students were instructed to use English in their group and whole-class deliberations. They were also instructed to use the appropriate language functions, structures, and expressions to achieve objectivity in their arguments. The study calls for further empirical research to verify the usefulness of the proposed learner-centered translation-based technique of teaching speaking展开更多
基金Funding was provided by the National Natural Science Foundation of China,82122017:Xiangjia Zhu,81970780:Yi Lu,81870642:Xiangjia Zhu,81670835:Yi LuScience and Technology Innovation Plan of Shanghai Science and Technology Commission,19441900700:Xiangjia Zhu,21S31904900:Xiangjia Zhu+3 种基金Shanghai Hospital Development Center,SHDC12019X08:Xiangjia ZhuShanghai Shenkang Hospital Development Center,SHDC2020CR4078:Xiangjia ZhuDouble-E Plan of Eye&ENT Hospital,SYA202006:Xiangjia ZhuShanghai Municipal Key Clinical Specialty Program,shslczdzk01901:Xiangjia Zhu.
文摘High myopia has long been highly prevalent worldwide with a largely yet unexplained genetic contribution.To identify novel susceptibility genes for axial length(AL)in highly myopic eyes,a genome-wide association study(GWAS)was performed using the genomic dataset of 350 deep whole-genome sequencing data from highly myopic patients.Top single nucleotide polymorphisms(SNPs)were functionally annotated.Immunofluorescence staining,quantitative polymerase chain reaction,and western blot were performed using neural retina of form-deprived myopic mice.Enrichment analyses were further performed.We identified the four top SNPs and found that ADAM Metallopeptidase With Thrombospondin Type 1 Motif 16(ADAMTS16)and Phosphatidylinositol Glycan Anchor Biosynthesis Class Z(PIGZ)had the potential of clinical signifi-cance.Animal experiments confirmed that PIGZ expression could be observed and showed higher expression level in form-deprived mice,especially in the ganglion cell layer.The messenger RNA(mRNA)levels of both ADAMTS16 and PIGZ were significantly higher in the neural retina of form-deprived eyes(p=0.005 and 0.007 respectively),and both proteins showed significantly upregulated expression in the neural retina of deprived eyes(p=0.004 and 0.042,respectively).Enrichment analysis revealed a significant role of cellular adhesion and signal transduction in AL,and also several AL-related pathways including circadian entrainment and inflammatory mediator regulation of transient receptor potential channels were proposed.In conclusion,the current study identified four novel SNPs associated with AL in highly myopic eyes and confirmed that the expression of ADAMTS16 and PIGZ was significantly upregulated in neural retina of deprived eyes.Enrichment analyses provided novel insight into the etiology of high myopia and opened avenues for future research interest.
文摘This study views pedagogical translation as a means of developing language skills, especially oral communication. Contrary to the claim that translation focuses only on reading and writing, this study presents anecdotal evidence showing that a learner-centered approach to translation can help foreign language students improve their speaking skill through group work, problem-solving, and cooperative learning. To this end, the paper presents practical examples of lexical, structural, textual, and cultural issues that students discussed in their process-oriented Arabic-English-Arabic pedagogical translation courses at the university level. The students were divided into groups of three or four to work together at all stages of the translation process. They spent most of the class time comprehending the source text, translating it, and discussing each other＇s target versions. In the remaining class time, the whole class discussed a target text translated by any one of the groups. The students were instructed to use English in their group and whole-class deliberations. They were also instructed to use the appropriate language functions, structures, and expressions to achieve objectivity in their arguments. The study calls for further empirical research to verify the usefulness of the proposed learner-centered translation-based technique of teaching speaking
