The diversity of sulfate-reducing bacteria （SRB） from deep layers of deep-sea sediments [ more than 2 m bsf （below seafloor） ] of two sites （WO1 -3 and WPO1 -4） in a tropical West Pacific warm pool region was ch...The diversity of sulfate-reducing bacteria （SRB） from deep layers of deep-sea sediments [ more than 2 m bsf （below seafloor） ] of two sites （WO1 -3 and WPO1 -4） in a tropical West Pacific warm pool region was characterized by using molecular phylogenetic analysis. The results of culture-independent samples demonstrated that the dominant clones from both sites were related to Grampositive spore forming genus, Desulfotomaculum, which accounted for 36.8% of all the sequencing clones from Site WP01 - 3 and 62.8% from Site WP01 -4. However, the other SRB group which was generally reported to be predominant in the deep-sea sediments of other regions, δ- subclass of the proteobacteria was found to be in very low percentages. Therefore, it could be speculated that there existed a unique chemical environment in the deep-sea sediment of this warm pool region. When comparing the Desulfotomaculum sp. related sequences from both sites, it was revealed that though the Desulfotomaculum-like sequences from Site WP01 -3 were more diverse than those from Site WP01 -4, all these sequences from both sites showed high similarity and formed a new phylogenetically homogeneous cluster in the Desulfotomaculum genus which had never been reported before. Successful enrichment of SRB was only achieved from samples of Site WP01 -4 and the sequence analysis of culture-dependent samples further confirmed the dominance of Desulfotomaculum genus. But Desulfotomaculum-related sequences from culture-dependent and culture-independent samples belonged to two different clusters respectively. This difference showed the choice of cultivation to the microorganisms.展开更多
为了研究西太平洋暖池核心区表层古菌群落结构及多样性,应用16S rDNA文库技术对KX08-973-22站位(取名为CWP)水体样构建基因文库,得到了561个克隆,经分析处理后得到50个OUT(OperationalTaxoni mic Units)。通过对16SrRNA基因序列的同源...为了研究西太平洋暖池核心区表层古菌群落结构及多样性,应用16S rDNA文库技术对KX08-973-22站位(取名为CWP)水体样构建基因文库,得到了561个克隆,经分析处理后得到50个OUT(OperationalTaxoni mic Units)。通过对16SrRNA基因序列的同源性比较,构建系统发育树分析及统计学分析,得出的结果:本次研究中的古菌类群都属于广古菌(Euryarchaeota)和泉古(Crenarchaeota)。广古菌以marine group II(MG II)(49.0%)为主,marine group III(MGIII)占一小部分(5.7%)。泉古菌以Marine Group I(MG I)为主(45.1%),Miscellaneous Crenarchaeotic Group(MCG)占极少数(0.2%)。本次研究的西太平洋暖池核心区表层古菌多样性不高,群落结构在小尺度上存在垂直变化。展开更多
文摘The diversity of sulfate-reducing bacteria （SRB） from deep layers of deep-sea sediments [ more than 2 m bsf （below seafloor） ] of two sites （WO1 -3 and WPO1 -4） in a tropical West Pacific warm pool region was characterized by using molecular phylogenetic analysis. The results of culture-independent samples demonstrated that the dominant clones from both sites were related to Grampositive spore forming genus, Desulfotomaculum, which accounted for 36.8% of all the sequencing clones from Site WP01 - 3 and 62.8% from Site WP01 -4. However, the other SRB group which was generally reported to be predominant in the deep-sea sediments of other regions, δ- subclass of the proteobacteria was found to be in very low percentages. Therefore, it could be speculated that there existed a unique chemical environment in the deep-sea sediment of this warm pool region. When comparing the Desulfotomaculum sp. related sequences from both sites, it was revealed that though the Desulfotomaculum-like sequences from Site WP01 -3 were more diverse than those from Site WP01 -4, all these sequences from both sites showed high similarity and formed a new phylogenetically homogeneous cluster in the Desulfotomaculum genus which had never been reported before. Successful enrichment of SRB was only achieved from samples of Site WP01 -4 and the sequence analysis of culture-dependent samples further confirmed the dominance of Desulfotomaculum genus. But Desulfotomaculum-related sequences from culture-dependent and culture-independent samples belonged to two different clusters respectively. This difference showed the choice of cultivation to the microorganisms.
文摘为了研究西太平洋暖池核心区表层古菌群落结构及多样性,应用16S rDNA文库技术对KX08-973-22站位(取名为CWP)水体样构建基因文库,得到了561个克隆,经分析处理后得到50个OUT(OperationalTaxoni mic Units)。通过对16SrRNA基因序列的同源性比较,构建系统发育树分析及统计学分析,得出的结果:本次研究中的古菌类群都属于广古菌(Euryarchaeota)和泉古(Crenarchaeota)。广古菌以marine group II(MG II)(49.0%)为主,marine group III(MGIII)占一小部分(5.7%)。泉古菌以Marine Group I(MG I)为主(45.1%),Miscellaneous Crenarchaeotic Group(MCG)占极少数(0.2%)。本次研究的西太平洋暖池核心区表层古菌多样性不高,群落结构在小尺度上存在垂直变化。
