A new Arthrospira strain named Arthrospira sp.ZJWST-S1 was isolated from a local digested piggery wastewater(DPW)storage pool in Jiaxing City,Zhejiang Province,China.It possessed good stain resistance against contamin...A new Arthrospira strain named Arthrospira sp.ZJWST-S1 was isolated from a local digested piggery wastewater(DPW)storage pool in Jiaxing City,Zhejiang Province,China.It possessed good stain resistance against contaminants in DPW,which was pretreated with a membrane bioreactor(MBR).The strain was identified as Arthrospira platensis(A.platensis)based on its morphological characteristics and the 16S rDNA sequencing analysis.The effect of chrominance on growth of A.platensis ZJWST-S1 was investigated in a field raceway pond filled with MBR effluent or MBR effluent decolorized with ozonation.After ozonation,the chrominance of MBR effluent was decreased from 700 mg Pt/L to 150 mg Pt/L.Two runs of cultivation showed that A.platensis ZJWST-S1 grew faster in the ozone decolorized MBR effluent,the averaged biomass concentration being 0.907 g/L after 10 days of cultivation,close to that in a Zarrouk medium(0.969 g/L).By comparison,the biomass grew much slower in the non-decolorized MBR effluent(0.624 g/L).The pollutant removal was also benefited from the accelerative growth of A.platensis ZJWST-S1 in the decolorized MBR effluent.Almost all ammonium,61.2%of nitrate and 68.1%of phosphate were removed by the A.platensis ZJWST-S1 in the decolorized MBR effluent,much higher than the corresponding values of almost all ammonium,25.4%of nitrate and 36.5%of phosphate in the MBR effluent.Furthermore,the Arthrospira biomass harvested from the ozone decolorized MBR effluent after 10 d cultivation was with crude protein content of 59.1%±3.5%in dry algae powder.The content of Pb,As,Cd and Hg in biomass was also low enough to meet the Chinese Arthrospira Standard for Animal Feed(GB/T 17243-1998).This study showed that the new strain A.platensis ZJWST-S1 possessed potential to be used for producing animal feed and simultaneous removal of nitrogen and phosphorus in DPW.展开更多
A biofilm membrane bioreactor （BF-MBR） and a conventional membrane bioreactor （MBR） were parallelly operated for treating digested piggery wastewater. The removal performance of COD, TN, NH4＋-N, TP as well as ant...A biofilm membrane bioreactor （BF-MBR） and a conventional membrane bioreactor （MBR） were parallelly operated for treating digested piggery wastewater. The removal performance of COD, TN, NH4＋-N, TP as well as antibiotics were simultaneously studied when the hydraulic retention time （HRT） was gradually shortened from 9 d to 1 d and when the ratio ofinfluent COD to TN was changed. The results showed that the effluent quality in both reactors was poor and unstable at an influent COD/ TN ratio of 1.0±0.2. The effluent quality was signifcantly improved as the influent COD/TN ratio was increased to 2.3±0.5. The averaged removal rates of COD, NH4＋-N, TN and TP were 92.1%, 97.1%, 35.6% and 54.2%, respectively, in the BF-MBR, significantly higher than the corresponding values of 91.7%, 90.9%, 17.4% and 31.9% in the MBR. Analysis of 11 typical veterinary antibiotics （from the tetracycline, sulfonamide, quinolone, and macrolide families） revealed that the BF-MBR removed more antibiotics than the MBR. Although the antibiotics removal decreased with a shortened HRT, high antibiotics removals of 86.8%, 80.2% and 45.3% were observed in the BF-MBR at HRT of 5~4 d, 3-2 d and 1 d, respectively, while the corresponding values were only 83.8%, 57.0% and 25.5% in the MBR. Moreover, the BF-MBR showed a 15% higher retention rate of antibiotics and consumed 40% less alkalinity than the MBR. Results above suggest that the BF-MBR was more suitable for digested piggery wastewater treatment.展开更多
基金Zhejiang Provincial Key Research Project(2014C03022).
文摘A new Arthrospira strain named Arthrospira sp.ZJWST-S1 was isolated from a local digested piggery wastewater(DPW)storage pool in Jiaxing City,Zhejiang Province,China.It possessed good stain resistance against contaminants in DPW,which was pretreated with a membrane bioreactor(MBR).The strain was identified as Arthrospira platensis(A.platensis)based on its morphological characteristics and the 16S rDNA sequencing analysis.The effect of chrominance on growth of A.platensis ZJWST-S1 was investigated in a field raceway pond filled with MBR effluent or MBR effluent decolorized with ozonation.After ozonation,the chrominance of MBR effluent was decreased from 700 mg Pt/L to 150 mg Pt/L.Two runs of cultivation showed that A.platensis ZJWST-S1 grew faster in the ozone decolorized MBR effluent,the averaged biomass concentration being 0.907 g/L after 10 days of cultivation,close to that in a Zarrouk medium(0.969 g/L).By comparison,the biomass grew much slower in the non-decolorized MBR effluent(0.624 g/L).The pollutant removal was also benefited from the accelerative growth of A.platensis ZJWST-S1 in the decolorized MBR effluent.Almost all ammonium,61.2%of nitrate and 68.1%of phosphate were removed by the A.platensis ZJWST-S1 in the decolorized MBR effluent,much higher than the corresponding values of almost all ammonium,25.4%of nitrate and 36.5%of phosphate in the MBR effluent.Furthermore,the Arthrospira biomass harvested from the ozone decolorized MBR effluent after 10 d cultivation was with crude protein content of 59.1%±3.5%in dry algae powder.The content of Pb,As,Cd and Hg in biomass was also low enough to meet the Chinese Arthrospira Standard for Animal Feed(GB/T 17243-1998).This study showed that the new strain A.platensis ZJWST-S1 possessed potential to be used for producing animal feed and simultaneous removal of nitrogen and phosphorus in DPW.
文摘A biofilm membrane bioreactor （BF-MBR） and a conventional membrane bioreactor （MBR） were parallelly operated for treating digested piggery wastewater. The removal performance of COD, TN, NH4＋-N, TP as well as antibiotics were simultaneously studied when the hydraulic retention time （HRT） was gradually shortened from 9 d to 1 d and when the ratio ofinfluent COD to TN was changed. The results showed that the effluent quality in both reactors was poor and unstable at an influent COD/ TN ratio of 1.0±0.2. The effluent quality was signifcantly improved as the influent COD/TN ratio was increased to 2.3±0.5. The averaged removal rates of COD, NH4＋-N, TN and TP were 92.1%, 97.1%, 35.6% and 54.2%, respectively, in the BF-MBR, significantly higher than the corresponding values of 91.7%, 90.9%, 17.4% and 31.9% in the MBR. Analysis of 11 typical veterinary antibiotics （from the tetracycline, sulfonamide, quinolone, and macrolide families） revealed that the BF-MBR removed more antibiotics than the MBR. Although the antibiotics removal decreased with a shortened HRT, high antibiotics removals of 86.8%, 80.2% and 45.3% were observed in the BF-MBR at HRT of 5~4 d, 3-2 d and 1 d, respectively, while the corresponding values were only 83.8%, 57.0% and 25.5% in the MBR. Moreover, the BF-MBR showed a 15% higher retention rate of antibiotics and consumed 40% less alkalinity than the MBR. Results above suggest that the BF-MBR was more suitable for digested piggery wastewater treatment.
