目的:探讨扩散加权成像(DWI)和T2WI体积分析诊断可切除直肠癌壁外血管侵犯(EMVI)和淋巴结转移(LNM)的效能及其相关性。方法:搜集于术前1周内行MRI检查的直肠癌患者95例,所有患者术后均行病理学检查,术前应用DWI和T2WI进行大体肿瘤体积(G...目的:探讨扩散加权成像(DWI)和T2WI体积分析诊断可切除直肠癌壁外血管侵犯(EMVI)和淋巴结转移(LNM)的效能及其相关性。方法:搜集于术前1周内行MRI检查的直肠癌患者95例,所有患者术后均行病理学检查,术前应用DWI和T2WI进行大体肿瘤体积(GTV)分析,判断两者的诊断效能,并与病理学EMVI和LNM进行相关性分析。结果:DWI和T2WI测量的直肠癌GTV与EMVI(r=0.812 vs r=0.726;P<0.0001)和LNM(r=0.821 vs r=0.761;P<0.0001)相关。单变量分析结果显示DWI和T2WI测量的GTV与EMVI和LNM相关(P值均<0.0001);多变量分析结果显示DWI测量的GTV为EMVI(OR=1.315,P=0.005)和LNM(OR=1.472,P=0.005)的独立危险因素。DWI和T2WI测量的GTV均可用于鉴别LNM N0与N1、N0与N1-N2、N0-N1与N2(P<0.0001);ROC曲线分析结果显示DWI测量的GTV鉴别EMVI(0.901 vs 0.890)、N0与N1(0.871 vs 0.833)、N0与N1-N2(0.931 vs 0.920)、N0-N1与N2(0.928 vs 0.916)的曲线下面积均高于T2WI。结论:可切除直肠癌DWI和T2WI测量的GTV与EMVI、LNM相关,DWI测量的GTV对于EMVI和LNM具有更高的诊断效能。展开更多
Near-infrared diffuse reflectance spectroscopy （NIRDRS） has attracted more and more attention in analyzing the components in samples with complex matrices. However, to apply this technique to micro-analysis, there a...Near-infrared diffuse reflectance spectroscopy （NIRDRS） has attracted more and more attention in analyzing the components in samples with complex matrices. However, to apply this technique to micro-analysis, there are still some obstacles to overcome such as the low sensitivity and spectral overlapping associated with this approach. A method for fast determination of bovine serum albumin （BSA） in micro-volume samples was studied using NIRDRS with sample spots and chemometric techniques. 10 p^L of sample spotted on a filter paper substrate was used for the spectral measurements. Quantitative analysis was obtained by partial least squares （PLS） regression with signal processing and variable selection. The results show that the correlation coefficient （R） between the predicted and the reference concentration is 0.9897 and the recoveries are in the range of 87.4%-114.4% for the validation samples in the concentration range of 0.61-8.10 mg/mL. These results suggest that the method has the potential to quickly measure proteins in micro-volume solutions.展开更多
文摘目的:探讨扩散加权成像(DWI)和T2WI体积分析诊断可切除直肠癌壁外血管侵犯(EMVI)和淋巴结转移(LNM)的效能及其相关性。方法:搜集于术前1周内行MRI检查的直肠癌患者95例,所有患者术后均行病理学检查,术前应用DWI和T2WI进行大体肿瘤体积(GTV)分析,判断两者的诊断效能,并与病理学EMVI和LNM进行相关性分析。结果:DWI和T2WI测量的直肠癌GTV与EMVI(r=0.812 vs r=0.726;P<0.0001)和LNM(r=0.821 vs r=0.761;P<0.0001)相关。单变量分析结果显示DWI和T2WI测量的GTV与EMVI和LNM相关(P值均<0.0001);多变量分析结果显示DWI测量的GTV为EMVI(OR=1.315,P=0.005)和LNM(OR=1.472,P=0.005)的独立危险因素。DWI和T2WI测量的GTV均可用于鉴别LNM N0与N1、N0与N1-N2、N0-N1与N2(P<0.0001);ROC曲线分析结果显示DWI测量的GTV鉴别EMVI(0.901 vs 0.890)、N0与N1(0.871 vs 0.833)、N0与N1-N2(0.931 vs 0.920)、N0-N1与N2(0.928 vs 0.916)的曲线下面积均高于T2WI。结论:可切除直肠癌DWI和T2WI测量的GTV与EMVI、LNM相关,DWI测量的GTV对于EMVI和LNM具有更高的诊断效能。
基金supported by the National Natural Science Foundation of China(No.20835002)
文摘Near-infrared diffuse reflectance spectroscopy （NIRDRS） has attracted more and more attention in analyzing the components in samples with complex matrices. However, to apply this technique to micro-analysis, there are still some obstacles to overcome such as the low sensitivity and spectral overlapping associated with this approach. A method for fast determination of bovine serum albumin （BSA） in micro-volume samples was studied using NIRDRS with sample spots and chemometric techniques. 10 p^L of sample spotted on a filter paper substrate was used for the spectral measurements. Quantitative analysis was obtained by partial least squares （PLS） regression with signal processing and variable selection. The results show that the correlation coefficient （R） between the predicted and the reference concentration is 0.9897 and the recoveries are in the range of 87.4%-114.4% for the validation samples in the concentration range of 0.61-8.10 mg/mL. These results suggest that the method has the potential to quickly measure proteins in micro-volume solutions.
