Purpose:To assess the efficacy and safety of a novel ophthalmic viscosurgical device-free(OVD-free)method for posterior chamber phakic intraocular lens(PIOL)implantation in myopic eyes.Methods:In this retrospective co...Purpose:To assess the efficacy and safety of a novel ophthalmic viscosurgical device-free(OVD-free)method for posterior chamber phakic intraocular lens(PIOL)implantation in myopic eyes.Methods:In this retrospective cohort study,the medical records of myopic eyes that underwent PIOL(Implantable Collamer Lens,ICL)implantation for myopia correction at the Eye Hospital of Wenzhou Medical University between May 2015 and March 2017 were reviewed.A total of 49 eyes with complete data that met follow up requirements(2 h,1 day,1 week,3 months postoperatively)were recruited.Based on the surgical techniques used,the eyes were divided into the OVD-free method group and the standard method group.The clinical data,including intraocular pressure(IOP),corrected distance visual acuity(CDVA)and spherical equivalent(SE),at each follow-up were collected for comparison.Endothelial cell loss and complications were also investigated.Results:Twenty-one eyes received the standard method,and 28 eyes received the OVD-free method.A rise in IOP>22 mmHg at 2 h was noted in 14 eyes(66.7%)in the standard group and none(0%)in the OVD-free group(p<0.001).The rise in IOP from baseline was significantly higher at 2 h in the standard group(10.5±5.2 mmHg vs.2.2±3.3 mmHg,difference:8.3,95%CI 5.8 to 10.8;p<0.001).There was a significant difference in the time course of LogMAR CDVA changes between the two groups(p=0.047).The LogMAR CDVA was significantly better in the OVD-free method group compared to the standard group at 1 day(−0.076,95%CI−0.134 to−0.018;p=0.012),1 week(−0.071,95%CI−0.135 to−0.007;p=0.03),but not at 3 months(−0.046,95%CI−0.107 to 0.015;p=0.134).There was no significant difference in the time course of SE changes between the two groups(p=0.471;p=0.705).In the OVD-free group,mean endothelial cell loss was 4.6%at 3 months(2522±281 vs.2407±226 cells/mm^(2),difference:-115,95%CI−295 to 65;p=0.187).No complications were reported in both groups except for the early IOP elevation in the standard group during the observat展开更多
AIM:To report the clinical prognosis and pathological findings of accidental lens vacuolar changes in eyes with intraoperative exposure to a dispersive ophthalmic viscosurgical device(OVD).METHODS:Two patients who dev...AIM:To report the clinical prognosis and pathological findings of accidental lens vacuolar changes in eyes with intraoperative exposure to a dispersive ophthalmic viscosurgical device(OVD).METHODS:Two patients who developed transient lens vacuolar changes during uneventful persistent pupillary membrane(PPM)removal surgery were presented and followed up.This event was speculated to be associated with an intraoperative dispersive OVD DisCoVisc(hyaluronic acid 1.6%-chondroitin sulfate 4.0%)exposure.Then,to provide the pathological basis for our speculation,another four cataract patients were randomly exposed to different OVDs,and their anterior lens capsules were investigated with transmission electron microscopy(TEM).RESULTS:After months,the subcapsular vacuoles in both PPM cases were gradually disappeared without visual deterioration.For the cataract patients,similar lens changes were observed intraoperatively in those exposed to a dispersive DisCoVisc but not a cohesive OVD IVIZ(sodium hyaluronate gel 1.0%).In addition,marked ultrastructural changes,including chromatin condensation,extensive cytoplasmic vacuoles,and obvious intercellular space between lens epithelial cells in the anterior lens capsules of all eyes exposed to DisCoVisc,were observed by TEM.CONCLUSION:The lens vacuolar changes may be associated with a dispersive OVD exposure.Therefore,it is not preferable to use dispersive OVDs in patients with transparent lenses or without the intention of lens extraction.In addition,close follow-ups instead of immediate lens extraction are recommended for the occurrence of similar lens lesions.展开更多
AIM： To compare characteristics of preloaded and nonpreloaded intraocular lens（IOL） delivery systems during IOL delivery procedures. METHODS： Total 101 human eyes were included in this prospective observational ca...AIM： To compare characteristics of preloaded and nonpreloaded intraocular lens（IOL） delivery systems during IOL delivery procedures. METHODS： Total 101 human eyes were included in this prospective observational case series. Delivery characteristics of 5 types of IOLs including i Sert250 NC60（NC60）, En Vista MX60（MX60）, Acry Sof IQ SN60 WF（SN60 WF）, TECNIS ZCB00（ZCB00）, and TECNIS PCB00（PCB00） were investigated. NC60 and PCB00 were injected via preloaded delivery systems and other IOLs were injected via nonpreloaded systems. In the human trial, time taken from IOL loading to completion of implantation was measured in all eyes undergoing conventional cataract surgery. Using 4 excised porcine eyes, dynamics of ophthalmic viscosurgical device（OVD） between an IOL injector and a porcine eye was analyzed using fluorescein sodiumstained OVD. RESULTS： The average time for IOL implantation was 22.0 s for NC60, 43.2 s for MX60, 32.3 s for SN60 WF, 41.4 s for ZCB00, and 14.6 s for PCB00 respectively. The number of cases with IOL manipulation with a second instrument was 6 for MX60, 2 for ZCB00, 0 for SN60 WF, NC60, and PCB00. Amount of OVD pushed into a porcine eye was smaller with a preloaded system than with non-preloaded systems. CONCLUSION： IOL delivery with preloaded systems is faster and more predictable. Moreover, a preloaded delivery system shows relatively less OVD pushed into a porcine eye than non-preloaded systems.展开更多
样品经50mmol·L^(-1) NaH_2PO_4-0.1mol·L^(-1) NaCl混合溶液溶解后,首先采用凝胶渗透色谱与多角度激光光散射联用仪确定透明质酸钠和硫酸软骨素钠的分子量,依据分子量分布确定水凝胶色谱柱(OH pak SB-806HQ L012028与OH pak ...样品经50mmol·L^(-1) NaH_2PO_4-0.1mol·L^(-1) NaCl混合溶液溶解后,首先采用凝胶渗透色谱与多角度激光光散射联用仪确定透明质酸钠和硫酸软骨素钠的分子量,依据分子量分布确定水凝胶色谱柱(OH pak SB-806HQ L012028与OH pak p8514-804L102028串联)为分离柱,OH pak SB-G G008146为保护柱,流动相采用50mmol·L^(-1) NaH_2PO_4-0.1mol·L^(-1) NaCl混合溶液,流量为0.5mL·min^(-1),以示差折光检测器进行测定。透明质酸钠和硫酸软骨素钠的质量浓度在0.10~1.0g·L^(-1)内与其峰面积呈线性关系,检出限(3S/N)分别为0.051,0.059g·L^(-1)。加标回收率在83.3%~110%之间,测定值的相对标准偏差(n=6)小于2.2%。展开更多
基金This study is supported by the National Natural Science Foundation of China(grant numbers 81570869)Zhejiang Provincial Natural Science Foundation of China(grant numbers Y2110784)+4 种基金Zhejiang Provincial Foundation of China for Distinguished Young Talents in Medicine and Health(grant numbers 2010QNA018)Wenzhou Key Team of Scientific and Technological Innovation(grant number C20170002)Foundation of Wenzhou City Science&Technology Bureau(grant numbers Y20140705)Engineering Development Project of Ophthalmology and Optometry(grant number GCKF201601)The funding organizations have no role in the design or conduct of this study.
文摘Purpose:To assess the efficacy and safety of a novel ophthalmic viscosurgical device-free(OVD-free)method for posterior chamber phakic intraocular lens(PIOL)implantation in myopic eyes.Methods:In this retrospective cohort study,the medical records of myopic eyes that underwent PIOL(Implantable Collamer Lens,ICL)implantation for myopia correction at the Eye Hospital of Wenzhou Medical University between May 2015 and March 2017 were reviewed.A total of 49 eyes with complete data that met follow up requirements(2 h,1 day,1 week,3 months postoperatively)were recruited.Based on the surgical techniques used,the eyes were divided into the OVD-free method group and the standard method group.The clinical data,including intraocular pressure(IOP),corrected distance visual acuity(CDVA)and spherical equivalent(SE),at each follow-up were collected for comparison.Endothelial cell loss and complications were also investigated.Results:Twenty-one eyes received the standard method,and 28 eyes received the OVD-free method.A rise in IOP>22 mmHg at 2 h was noted in 14 eyes(66.7%)in the standard group and none(0%)in the OVD-free group(p<0.001).The rise in IOP from baseline was significantly higher at 2 h in the standard group(10.5±5.2 mmHg vs.2.2±3.3 mmHg,difference:8.3,95%CI 5.8 to 10.8;p<0.001).There was a significant difference in the time course of LogMAR CDVA changes between the two groups(p=0.047).The LogMAR CDVA was significantly better in the OVD-free method group compared to the standard group at 1 day(−0.076,95%CI−0.134 to−0.018;p=0.012),1 week(−0.071,95%CI−0.135 to−0.007;p=0.03),but not at 3 months(−0.046,95%CI−0.107 to 0.015;p=0.134).There was no significant difference in the time course of SE changes between the two groups(p=0.471;p=0.705).In the OVD-free group,mean endothelial cell loss was 4.6%at 3 months(2522±281 vs.2407±226 cells/mm^(2),difference:-115,95%CI−295 to 65;p=0.187).No complications were reported in both groups except for the early IOP elevation in the standard group during the observat
基金Supported by the National Key R&D Program of China(No.2020YFC2008200)the National Natural Science Foundation of China(No.81970778,No.82271066,No.81970813)+1 种基金the Natural Science Foundation of Guangdong Province(No.2023A1515011198)Guangzhou Municipal Science and Technology Project(No.SL2022A03J00553).
文摘AIM:To report the clinical prognosis and pathological findings of accidental lens vacuolar changes in eyes with intraoperative exposure to a dispersive ophthalmic viscosurgical device(OVD).METHODS:Two patients who developed transient lens vacuolar changes during uneventful persistent pupillary membrane(PPM)removal surgery were presented and followed up.This event was speculated to be associated with an intraoperative dispersive OVD DisCoVisc(hyaluronic acid 1.6%-chondroitin sulfate 4.0%)exposure.Then,to provide the pathological basis for our speculation,another four cataract patients were randomly exposed to different OVDs,and their anterior lens capsules were investigated with transmission electron microscopy(TEM).RESULTS:After months,the subcapsular vacuoles in both PPM cases were gradually disappeared without visual deterioration.For the cataract patients,similar lens changes were observed intraoperatively in those exposed to a dispersive DisCoVisc but not a cohesive OVD IVIZ(sodium hyaluronate gel 1.0%).In addition,marked ultrastructural changes,including chromatin condensation,extensive cytoplasmic vacuoles,and obvious intercellular space between lens epithelial cells in the anterior lens capsules of all eyes exposed to DisCoVisc,were observed by TEM.CONCLUSION:The lens vacuolar changes may be associated with a dispersive OVD exposure.Therefore,it is not preferable to use dispersive OVDs in patients with transparent lenses or without the intention of lens extraction.In addition,close follow-ups instead of immediate lens extraction are recommended for the occurrence of similar lens lesions.
基金Supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) by the Ministry of Education, Science and Technology (No.NRF2016R1A2B4009626)
文摘AIM： To compare characteristics of preloaded and nonpreloaded intraocular lens（IOL） delivery systems during IOL delivery procedures. METHODS： Total 101 human eyes were included in this prospective observational case series. Delivery characteristics of 5 types of IOLs including i Sert250 NC60（NC60）, En Vista MX60（MX60）, Acry Sof IQ SN60 WF（SN60 WF）, TECNIS ZCB00（ZCB00）, and TECNIS PCB00（PCB00） were investigated. NC60 and PCB00 were injected via preloaded delivery systems and other IOLs were injected via nonpreloaded systems. In the human trial, time taken from IOL loading to completion of implantation was measured in all eyes undergoing conventional cataract surgery. Using 4 excised porcine eyes, dynamics of ophthalmic viscosurgical device（OVD） between an IOL injector and a porcine eye was analyzed using fluorescein sodiumstained OVD. RESULTS： The average time for IOL implantation was 22.0 s for NC60, 43.2 s for MX60, 32.3 s for SN60 WF, 41.4 s for ZCB00, and 14.6 s for PCB00 respectively. The number of cases with IOL manipulation with a second instrument was 6 for MX60, 2 for ZCB00, 0 for SN60 WF, NC60, and PCB00. Amount of OVD pushed into a porcine eye was smaller with a preloaded system than with non-preloaded systems. CONCLUSION： IOL delivery with preloaded systems is faster and more predictable. Moreover, a preloaded delivery system shows relatively less OVD pushed into a porcine eye than non-preloaded systems.
文摘样品经50mmol·L^(-1) NaH_2PO_4-0.1mol·L^(-1) NaCl混合溶液溶解后,首先采用凝胶渗透色谱与多角度激光光散射联用仪确定透明质酸钠和硫酸软骨素钠的分子量,依据分子量分布确定水凝胶色谱柱(OH pak SB-806HQ L012028与OH pak p8514-804L102028串联)为分离柱,OH pak SB-G G008146为保护柱,流动相采用50mmol·L^(-1) NaH_2PO_4-0.1mol·L^(-1) NaCl混合溶液,流量为0.5mL·min^(-1),以示差折光检测器进行测定。透明质酸钠和硫酸软骨素钠的质量浓度在0.10~1.0g·L^(-1)内与其峰面积呈线性关系,检出限(3S/N)分别为0.051,0.059g·L^(-1)。加标回收率在83.3%~110%之间,测定值的相对标准偏差(n=6)小于2.2%。
