Objective:To investigate the anti-diabetic and anti-cholesterolemic activity of methanol extracts of leaves of Amaranthus caudatus,Amaranthus spinosus and Amaranthus viridis in normal and streptozotocin(STZ) induced d...Objective:To investigate the anti-diabetic and anti-cholesterolemic activity of methanol extracts of leaves of Amaranthus caudatus,Amaranthus spinosus and Amaranthus viridis in normal and streptozotocin(STZ) induced diabetic rats.Methods:In this study,the anti-diabetic and anti-cholesterolemic activity of methanol extracts of leaves of all three plants was evaluated by using normal and STZ induced diabetic rots at a dose of 200 mg/kg and 400 mg/kg p.o.daily for 21 days.Blood glucose levels and body weight were monitored at specific intervals,and different biochemical parameters,serum cholesterol,serum triglyceride,high density lipoprotein, low density lipoprotein and very low density lipoprotein were also assessed in the experimental animals.Histology of pancreas was performed.Results:It was found that all the three plants at 400 mg/kg dose showed significant anti-diabetic and anti-cholesterolemic activity(P<0.01),while at 200 mg/kg dose less significant anti-diabetic activity(P<0.05) was observed.Concluslons: Methanol extracts of Amaranthus caudatus,Amaranlhus spinosus and Amaranthus viridis showed significant anti-diabetic and anti-cholesterolemic activity,which provides the scientific proof for their traditional claims.展开更多
Oceanic uptake of anthropogenic carbon dioxide results in a decrease in seawater pH, a process known as "ocean acidification". The pearl oyster Pinctada fucata, the noble scallop Chlamys nobilis, and the gre...Oceanic uptake of anthropogenic carbon dioxide results in a decrease in seawater pH, a process known as "ocean acidification". The pearl oyster Pinctada fucata, the noble scallop Chlamys nobilis, and the green-lipped mussel Perna viridis are species of economic and ecological importance along the southern coast of China. We evaluated the effects of seawater acidification on clearance, respiration, and excretion rates in these three species. The animals were reared in seawater at pH 8.1 (control), 7.7, or 7.4. The clearance rate was highest at pH 7.7 for P. fucata and at pH 8.1 for C. nobilis and P. viridis. The pH had little effecton the respiration rate of P. fucata and P. viridis. In contrast, the respiration rate was significantly lower atpH 7.4 in C. nobilis. The excretion rate was significantly lower at pH 7.4 than pH 8.1 for all species. Theresults indicate that the reduction in seawater pH likely affected the metabolic process (food intake, oxygenconsumption, and ammonia excretion) of these bivalves. Different species respond differently to seawateracidification. Further studies are needed to demonstrate the exact mechanisms for this effect and evaluateadaptability of these bivalves to future acidified oceans.展开更多
Pollution of marine environment has become an issue of major concern in recent years. Serious environmental pollution by heavy metals results from their increasing utilization in industrial processes and because most ...Pollution of marine environment has become an issue of major concern in recent years. Serious environmental pollution by heavy metals results from their increasing utilization in industrial processes and because most heavy metals are transported into the marine environment and accumulated without decomposition. The aim of the present study is to investigate the effects on growth, pigments, lipid peroxidation, and some antioxidant enzyme activities of marine microalga Pavlova viridis, in response to elevated concentrations of cobalt （Co） and manganese （Mn）, especially with regard to the involvement of antioxidative defences against heavy metal-induced oxidative stress. In response to Co^2＋, lipid peroxidation was enhanced compared to the control, as an indication of the oxidative damage caused by metal concentration assayed in the microalgal cells but not Mn^2＋. Exposure of Pavlova viridis to the two metals caused changes in enzyme activities in a different manner, depending on the metal assayed： after Co^2＋ treatments, total superoxide dismutase （SOD） activity was irregular, although it was not significantly affected by Mn^2＋ exposure. Co^2＋ and Mn^2＋ stimulated the activities of catalase （CAT） and glutathione （GSH）, whereas, glutathione peroxidase （GPX） showed a remarkable increase in activity in response to Co^2＋ treatments and decreased gradually with Mn^2＋ concentration, up to 50 μmol/L, and then rose very rapidly, reaching to about 38.98% at 200 μmol/L Mn^2＋. These results suggest that an activation of some antioxidant enzymes was enhanced, to counteract the oxidative stress induced by the two metals at higher concentration.展开更多
Wastewater stabilization ponds generate low cost by-products that are useful for agriculture. The utilization of these by-products for soil amendment and as a source of nutrients for plants requires a high level of sa...Wastewater stabilization ponds generate low cost by-products that are useful for agriculture. The utilization of these by-products for soil amendment and as a source of nutrients for plants requires a high level of sanitation and stabilization of the organic matter, to maintain acceptable levels of soil, water and air quality. In this study, two aquaculture wastewater treatment systems; recirculating system and a floating plant bed system were designed to improve the quality of irrigation water in local communities with low income. In both systems the grass species Lolium perenne Lam was used as a plant biofilter while vegetable specie Amaranthus viridis was used to evaluate the performance of the system and the suitability of the phyto-treated water for irrigation. It was found that the harmful material removal rate for recirculating system was 88.9% for TAN （total ammonia nitrogen）, 90% for NO2--N, 64.8% for NO3--N while for floating plant bed system 82.7% for TAN, 82% for NO2--N and 60.5% for NO3--N. Comparative analysis of the efficiency of waste element removal between the two systems revealed that both systems performed well, however, plant growth was not robust for floating plant bed system while recirculating system is energy consuming. Although both systems did not attain sufficient levels of TN （total nitrogen） and TP （total phosphorus） load reduction, the treatment with L. perenne remarkably improved the irrigation water quality. A. viridis plants irrigated with the phyto-treated discharge water had lesser concentrations of heavy metals in their tissues compared to those irrigated with untreated discharge. The control plants irrigated with untreated discharge were also found to be highly lignified with few stems and small leaves.展开更多
This research was carried out to study the heavy metals removal like arsenic (As), lead (Pb), cadmium (Cd), nickel (Ni) and mercury (Hg) from Perna viridis. The trisodium citrate with Ru/Mn (25:75)-Al2O3 catalyst were...This research was carried out to study the heavy metals removal like arsenic (As), lead (Pb), cadmium (Cd), nickel (Ni) and mercury (Hg) from Perna viridis. The trisodium citrate with Ru/Mn (25:75)-Al2O3 catalyst were used for the treatment medium with optimum treatment conditions at 500 μg/g of dosage, at temperature of 35°C ± 1°C, at pH 7, for 5 hours of duration and in the presence of compressed air (flow rate 895.50 mL/min). The introduction of Ru/Mn (25:75)-Al2O3 catalyst at 100°C calcination temperature on the trisodium citrate medium indicates the reduction of heavy metals which achieved the permissible limit by Malaysian Food Regulations (1985) and Commission Regulation of EU (2006). The original concentrations of As: 1.65 ± 0.89 μg/g, Hg: 12.42 ± 0.53 μg/g, Pb: 6.25 ± 0.69 μg/g, Cd: 1.27 ± 0.25 μg/g and Ni: 2.00 ± 1.05 μg/g were reduced to As: 0.75 ± 0.04 μg/g (54.34%), Hg: 0.4 ± 0.02 μg/g (96.79%), Pb: 1.07 ± 0.02 μg/g (82.89%), Cd: 0.32 ± 0.10 μg/g (75.02%) and Ni: 0.76 ± 0.01 μg/g (61.83%). ICP-MS and MHS-AAS techniques were used for the metals analysis and the catalyst was characterized through X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-Ray (EDX) and Nitrogen Absorption (NA).展开更多
Green foxtail （Setaria viridis L.） is a common weed species in temperate regions. Research on the effect of delayed reproduction on the phenotypic plasticity and regularity of the vegetative and reproductive growth ...Green foxtail （Setaria viridis L.） is a common weed species in temperate regions. Research on the effect of delayed reproduction on the phenotypic plasticity and regularity of the vegetative and reproductive growth is of vital significance for understanding population regulation and control of the weed in the growing season. Green foxtail seeds were sown every 10 days from 25 June to 24 August of 2004. The growth and production metrics were measured via harvesting tufts and statistical analysis was carried out. The results showed that the reproductive tillers, seed number, seed biomass and one thousand-seed weight of plants at the first sowing （25 June） approximately increased 28.8, 7 827.0, 1 104,0 and 12.3 times compared with that at the last sowing （24 August）, respectively. Total tillers, reproductive tillers and height increased linearly as the reproductive period delayed, however, biomass increased exponentially. Quadratic equations best explained the relationships between the delayed reproductive period and seed number, inflorescence length, one thousand-seed weight, seed biomass. Based on the quantity and quality of seed production, weeding young seedlings emerging before July can be the most effective weed-control strategy in the Songnen Plain.展开更多
Green-lipped mussels (Perna viridis) were collected from a local mariculture site and placed in pre-cleaned sea water tanks containing 0, 0.2, 0.5 and 1.3 μg/ml CdCl2. The level of Cd in the gill, viscera and gonad w...Green-lipped mussels (Perna viridis) were collected from a local mariculture site and placed in pre-cleaned sea water tanks containing 0, 0.2, 0.5 and 1.3 μg/ml CdCl2. The level of Cd in the gill, viscera and gonad was measured. The concentration of Cd in the 3 tissues increased linearly over 4 days' of exposure. The rate of uptake depends on the concentration of Cd in the water. In the 3 tissues studied, the maximum rate of uptake (Vmax) was highest in the gill (500μg/g dry weight/day), followed by viscera (100μg/g dry weight/day) and gonad (56.8 μg/g dry weight/day). The majority of the Cd taken up was bound to a fraction of the heat-stable proteins similar to metallothioneins. The rate of Cd depuration from the tissues was poor. There was no change in Cd concentration over 8 days' depuration in clean water展开更多
In the present study, two new trinor-guaiane sesquiterpenes, named clavuridins B(1), and A(2), along with three known sesquiterpenes(3-5), were isolated from the Xisha soft coral Clavularia viridis. Their structures a...In the present study, two new trinor-guaiane sesquiterpenes, named clavuridins B(1), and A(2), along with three known sesquiterpenes(3-5), were isolated from the Xisha soft coral Clavularia viridis. Their structures and absolute configurations were determined on the basis of spectroscopic analysis, X-ray diffraction analysis with Cu Kα radiation and by comparison with related model compounds. Compounds 1 and 3–5 were evaluated for their cytotoxic activity.展开更多
文摘Objective:To investigate the anti-diabetic and anti-cholesterolemic activity of methanol extracts of leaves of Amaranthus caudatus,Amaranthus spinosus and Amaranthus viridis in normal and streptozotocin(STZ) induced diabetic rats.Methods:In this study,the anti-diabetic and anti-cholesterolemic activity of methanol extracts of leaves of all three plants was evaluated by using normal and STZ induced diabetic rots at a dose of 200 mg/kg and 400 mg/kg p.o.daily for 21 days.Blood glucose levels and body weight were monitored at specific intervals,and different biochemical parameters,serum cholesterol,serum triglyceride,high density lipoprotein, low density lipoprotein and very low density lipoprotein were also assessed in the experimental animals.Histology of pancreas was performed.Results:It was found that all the three plants at 400 mg/kg dose showed significant anti-diabetic and anti-cholesterolemic activity(P<0.01),while at 200 mg/kg dose less significant anti-diabetic activity(P<0.05) was observed.Concluslons: Methanol extracts of Amaranthus caudatus,Amaranlhus spinosus and Amaranthus viridis showed significant anti-diabetic and anti-cholesterolemic activity,which provides the scientific proof for their traditional claims.
基金Supported by National Natural Science Foundation of China (No.41006090)the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-YW-Q07-03)the National High Technology Research and Development Program of China (863 Program) (No.2006AA10A409)
文摘Oceanic uptake of anthropogenic carbon dioxide results in a decrease in seawater pH, a process known as "ocean acidification". The pearl oyster Pinctada fucata, the noble scallop Chlamys nobilis, and the green-lipped mussel Perna viridis are species of economic and ecological importance along the southern coast of China. We evaluated the effects of seawater acidification on clearance, respiration, and excretion rates in these three species. The animals were reared in seawater at pH 8.1 (control), 7.7, or 7.4. The clearance rate was highest at pH 7.7 for P. fucata and at pH 8.1 for C. nobilis and P. viridis. The pH had little effecton the respiration rate of P. fucata and P. viridis. In contrast, the respiration rate was significantly lower atpH 7.4 in C. nobilis. The excretion rate was significantly lower at pH 7.4 than pH 8.1 for all species. Theresults indicate that the reduction in seawater pH likely affected the metabolic process (food intake, oxygenconsumption, and ammonia excretion) of these bivalves. Different species respond differently to seawateracidification. Further studies are needed to demonstrate the exact mechanisms for this effect and evaluateadaptability of these bivalves to future acidified oceans.
基金Project supported by the Natural Science Foundation of Jiangsu Province(No.95021301)China Postdoctoral Science Foundation(No.2005037121)Jiangsu Planned Projects for Postdoctoral Research Funds(No.0401001C)
文摘Pollution of marine environment has become an issue of major concern in recent years. Serious environmental pollution by heavy metals results from their increasing utilization in industrial processes and because most heavy metals are transported into the marine environment and accumulated without decomposition. The aim of the present study is to investigate the effects on growth, pigments, lipid peroxidation, and some antioxidant enzyme activities of marine microalga Pavlova viridis, in response to elevated concentrations of cobalt （Co） and manganese （Mn）, especially with regard to the involvement of antioxidative defences against heavy metal-induced oxidative stress. In response to Co^2＋, lipid peroxidation was enhanced compared to the control, as an indication of the oxidative damage caused by metal concentration assayed in the microalgal cells but not Mn^2＋. Exposure of Pavlova viridis to the two metals caused changes in enzyme activities in a different manner, depending on the metal assayed： after Co^2＋ treatments, total superoxide dismutase （SOD） activity was irregular, although it was not significantly affected by Mn^2＋ exposure. Co^2＋ and Mn^2＋ stimulated the activities of catalase （CAT） and glutathione （GSH）, whereas, glutathione peroxidase （GPX） showed a remarkable increase in activity in response to Co^2＋ treatments and decreased gradually with Mn^2＋ concentration, up to 50 μmol/L, and then rose very rapidly, reaching to about 38.98% at 200 μmol/L Mn^2＋. These results suggest that an activation of some antioxidant enzymes was enhanced, to counteract the oxidative stress induced by the two metals at higher concentration.
基金Project supported by the Technology Department Zhejiang Province of China(No. 2005C22060).
文摘Wastewater stabilization ponds generate low cost by-products that are useful for agriculture. The utilization of these by-products for soil amendment and as a source of nutrients for plants requires a high level of sanitation and stabilization of the organic matter, to maintain acceptable levels of soil, water and air quality. In this study, two aquaculture wastewater treatment systems; recirculating system and a floating plant bed system were designed to improve the quality of irrigation water in local communities with low income. In both systems the grass species Lolium perenne Lam was used as a plant biofilter while vegetable specie Amaranthus viridis was used to evaluate the performance of the system and the suitability of the phyto-treated water for irrigation. It was found that the harmful material removal rate for recirculating system was 88.9% for TAN （total ammonia nitrogen）, 90% for NO2--N, 64.8% for NO3--N while for floating plant bed system 82.7% for TAN, 82% for NO2--N and 60.5% for NO3--N. Comparative analysis of the efficiency of waste element removal between the two systems revealed that both systems performed well, however, plant growth was not robust for floating plant bed system while recirculating system is energy consuming. Although both systems did not attain sufficient levels of TN （total nitrogen） and TP （total phosphorus） load reduction, the treatment with L. perenne remarkably improved the irrigation water quality. A. viridis plants irrigated with the phyto-treated discharge water had lesser concentrations of heavy metals in their tissues compared to those irrigated with untreated discharge. The control plants irrigated with untreated discharge were also found to be highly lignified with few stems and small leaves.
文摘This research was carried out to study the heavy metals removal like arsenic (As), lead (Pb), cadmium (Cd), nickel (Ni) and mercury (Hg) from Perna viridis. The trisodium citrate with Ru/Mn (25:75)-Al2O3 catalyst were used for the treatment medium with optimum treatment conditions at 500 μg/g of dosage, at temperature of 35°C ± 1°C, at pH 7, for 5 hours of duration and in the presence of compressed air (flow rate 895.50 mL/min). The introduction of Ru/Mn (25:75)-Al2O3 catalyst at 100°C calcination temperature on the trisodium citrate medium indicates the reduction of heavy metals which achieved the permissible limit by Malaysian Food Regulations (1985) and Commission Regulation of EU (2006). The original concentrations of As: 1.65 ± 0.89 μg/g, Hg: 12.42 ± 0.53 μg/g, Pb: 6.25 ± 0.69 μg/g, Cd: 1.27 ± 0.25 μg/g and Ni: 2.00 ± 1.05 μg/g were reduced to As: 0.75 ± 0.04 μg/g (54.34%), Hg: 0.4 ± 0.02 μg/g (96.79%), Pb: 1.07 ± 0.02 μg/g (82.89%), Cd: 0.32 ± 0.10 μg/g (75.02%) and Ni: 0.76 ± 0.01 μg/g (61.83%). ICP-MS and MHS-AAS techniques were used for the metals analysis and the catalyst was characterized through X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-Ray (EDX) and Nitrogen Absorption (NA).
基金the National Natural Science Foundation of China (30770397and 30470272).
文摘Green foxtail （Setaria viridis L.） is a common weed species in temperate regions. Research on the effect of delayed reproduction on the phenotypic plasticity and regularity of the vegetative and reproductive growth is of vital significance for understanding population regulation and control of the weed in the growing season. Green foxtail seeds were sown every 10 days from 25 June to 24 August of 2004. The growth and production metrics were measured via harvesting tufts and statistical analysis was carried out. The results showed that the reproductive tillers, seed number, seed biomass and one thousand-seed weight of plants at the first sowing （25 June） approximately increased 28.8, 7 827.0, 1 104,0 and 12.3 times compared with that at the last sowing （24 August）, respectively. Total tillers, reproductive tillers and height increased linearly as the reproductive period delayed, however, biomass increased exponentially. Quadratic equations best explained the relationships between the delayed reproductive period and seed number, inflorescence length, one thousand-seed weight, seed biomass. Based on the quantity and quality of seed production, weeding young seedlings emerging before July can be the most effective weed-control strategy in the Songnen Plain.
文摘Green-lipped mussels (Perna viridis) were collected from a local mariculture site and placed in pre-cleaned sea water tanks containing 0, 0.2, 0.5 and 1.3 μg/ml CdCl2. The level of Cd in the gill, viscera and gonad was measured. The concentration of Cd in the 3 tissues increased linearly over 4 days' of exposure. The rate of uptake depends on the concentration of Cd in the water. In the 3 tissues studied, the maximum rate of uptake (Vmax) was highest in the gill (500μg/g dry weight/day), followed by viscera (100μg/g dry weight/day) and gonad (56.8 μg/g dry weight/day). The majority of the Cd taken up was bound to a fraction of the heat-stable proteins similar to metallothioneins. The rate of Cd depuration from the tissues was poor. There was no change in Cd concentration over 8 days' depuration in clean water
基金supported by the Natural Science Foundation of China(Nos.81520108028,21672230,41506187,and 816013016)the SKLDR/SIMM Project(No.SIMM1501ZZ-03)
文摘In the present study, two new trinor-guaiane sesquiterpenes, named clavuridins B(1), and A(2), along with three known sesquiterpenes(3-5), were isolated from the Xisha soft coral Clavularia viridis. Their structures and absolute configurations were determined on the basis of spectroscopic analysis, X-ray diffraction analysis with Cu Kα radiation and by comparison with related model compounds. Compounds 1 and 3–5 were evaluated for their cytotoxic activity.
