目的观察清热化瘀法联合纳米银凝胶治疗精囊炎的临床疗效。方法 2014年1月至2015年3月,选取住院精囊炎患者62例,按就诊顺序随机分为治疗组32例和对照组30例,治疗组(内外合治)内服清热化瘀中药200 m L,2次/天;同时予纳米银凝胶纳肛,1支/...目的观察清热化瘀法联合纳米银凝胶治疗精囊炎的临床疗效。方法 2014年1月至2015年3月,选取住院精囊炎患者62例,按就诊顺序随机分为治疗组32例和对照组30例,治疗组(内外合治)内服清热化瘀中药200 m L,2次/天;同时予纳米银凝胶纳肛,1支/天。对照组(內治)内服清热化瘀中药200 m L,2次/天。治疗3周后复查评价疗效。结果治疗组(内外合治)疗效明显优于对照组(內治)疗效。两组疗效比较差异极显著(P=0.002<0.01)。结论内服清热化瘀中药配合纳米银凝胶直肠给药治疗精囊炎,能明显提高临床疗效。展开更多
We aimed to establish a novel rat model of seminal vesiculitis that would provide an effective approach to investigate the pathogenesis of this disease in the future. Eight male rats received the same operation, durin...We aimed to establish a novel rat model of seminal vesiculitis that would provide an effective approach to investigate the pathogenesis of this disease in the future. Eight male rats received the same operation, during which the root of one of the two seminal vesicles was partly ligatured with sutures and the other vesicle was left in tact. The samples of seminal vesicles were harvested on the 8th day followi ng the operation. Hematoxylin and eosi n and Mass on's trichrome stains were used to observe the histopathology and the presenee of fibrous tissue in seminal vesicles, respectively. Immunoblotting and immunohistochemistry were applied to determine the tumor n ecrosis factor-alpha and cyclooxygenase-2 levels in semi nal vesicle tissues. Real-time fluoresce nee qua ntitative polymerase chain reaction was performed to measure the gene expression levels of proinflammatory cytokines. H2O2 levels in the seminal plasma from the seminal vesicle were also measured. Hematoxylin and eosin staining suggested that there was inflammatory cell in filtration into the semi nal vesicles treated by partial root ligati on. The tumor n ecrosis factor-alpha and cyclooxygenase-2 proteins were significantly upregulated in the treated seminal vesicles. The tumor necrosis factor-alpha, cyclooxygenase, interleukin 6, and inducible nitric oxide synthase mRNA expression levels were also upregulated in the treated seminal vesicles. The H2O2 levels in the seminal plasma from seminal vesicles with partial root ligation were significantly elevated compared with those from vesicle left intact. In conclusion, partially ligating the root of the seminal vesicle via sutures in rats is an effective method to establish a seminal vesiculitis rat model.展开更多
文摘目的观察清热化瘀法联合纳米银凝胶治疗精囊炎的临床疗效。方法 2014年1月至2015年3月,选取住院精囊炎患者62例,按就诊顺序随机分为治疗组32例和对照组30例,治疗组(内外合治)内服清热化瘀中药200 m L,2次/天;同时予纳米银凝胶纳肛,1支/天。对照组(內治)内服清热化瘀中药200 m L,2次/天。治疗3周后复查评价疗效。结果治疗组(内外合治)疗效明显优于对照组(內治)疗效。两组疗效比较差异极显著(P=0.002<0.01)。结论内服清热化瘀中药配合纳米银凝胶直肠给药治疗精囊炎,能明显提高临床疗效。
文摘We aimed to establish a novel rat model of seminal vesiculitis that would provide an effective approach to investigate the pathogenesis of this disease in the future. Eight male rats received the same operation, during which the root of one of the two seminal vesicles was partly ligatured with sutures and the other vesicle was left in tact. The samples of seminal vesicles were harvested on the 8th day followi ng the operation. Hematoxylin and eosi n and Mass on's trichrome stains were used to observe the histopathology and the presenee of fibrous tissue in seminal vesicles, respectively. Immunoblotting and immunohistochemistry were applied to determine the tumor n ecrosis factor-alpha and cyclooxygenase-2 levels in semi nal vesicle tissues. Real-time fluoresce nee qua ntitative polymerase chain reaction was performed to measure the gene expression levels of proinflammatory cytokines. H2O2 levels in the seminal plasma from the seminal vesicle were also measured. Hematoxylin and eosin staining suggested that there was inflammatory cell in filtration into the semi nal vesicles treated by partial root ligati on. The tumor n ecrosis factor-alpha and cyclooxygenase-2 proteins were significantly upregulated in the treated seminal vesicles. The tumor necrosis factor-alpha, cyclooxygenase, interleukin 6, and inducible nitric oxide synthase mRNA expression levels were also upregulated in the treated seminal vesicles. The H2O2 levels in the seminal plasma from seminal vesicles with partial root ligation were significantly elevated compared with those from vesicle left intact. In conclusion, partially ligating the root of the seminal vesicle via sutures in rats is an effective method to establish a seminal vesiculitis rat model.
