Aims Myriophyllum spicatum and Hydrilla verticillata are common submerged macrophytes in the Yangtze River basin.To investigate their tolerances and adaptations to water-level fluctuations,an experiment was conducted ...Aims Myriophyllum spicatum and Hydrilla verticillata are common submerged macrophytes in the Yangtze River basin.To investigate their tolerances and adaptations to water-level fluctuations,an experiment was conducted in a pond.Methods We designed five different amplitudes of water-level fluctuations as static,615,630,660 and 690 cm during the 74 days of the experiment.In each amplitude treatment,two cultivation methods were examined as monoculture and mixed culture.Important Findings The results showed that M.spicatum had greater morphological responses to water-level fluctuations than H.verticillata.Fluctuating amplitude had significant effects on branch number,shoot length and root dry weight(DW)of M.spicatum,whereas it only had significant effect on branch number of H.verticillata.Both fluctuating amplitude and cultivation method had significant effects on total DW of M.spicatum,which was higher in monoculture than mixed culture.The total DW of H.verticillata was affected by fluctuating amplitude only,and the largest biomass was in the amplitude of 630 cm.Fruit DW of M.spicatum was largest in the amplitude of 630 cm,high amplitude of water-level fluctuations would inhibit flowering and seed production.Root DW proportion was significantly affected by fluctuating amplitude and cultivation method in both species,and the root DW proportion of M.spicatum was significantly higher in the amplitude-of 690 cm.We conclude that moderate amplitude of water-level fluctuations can promote the distribution and growth of both species,and in order to accelerate the restoration of both species in natural habitats,the optimum amplitude should be keep at 615 to 630 cm.展开更多
Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10umol/L Cd, 5 ...Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10umol/L Cd, 5 umol/L Hg, and 20 umol/L Cu for 96 h, showed changes in chlorophyll, protein content, and in DNA profiles. The changes in DNA profiles included variation in band intensity, presence or absence of certain bands and even appearance of new bands. Genomic template stability test performed for the qualitative measurement of changes in randomly amplified polymorphic DNA （RAPD） profiles, showed significant effect at the given concentration of metals. Cloning and sequencing of bands suggested that these markers although may not be homologous to any known gene but its conversion as a sequence characterized amplified region （SCAR） marker is useful in detecting the effects of genotoxin agents.展开更多
Hydrilla verticillata （Linn. f.） Royle is a submerged plant for phytoremediation on Taihu Lake, China. The planted submerged plant should be harvested in every autumn to reduce the nutrition burden of the lake. Five...Hydrilla verticillata （Linn. f.） Royle is a submerged plant for phytoremediation on Taihu Lake, China. The planted submerged plant should be harvested in every autumn to reduce the nutrition burden of the lake. Five compounds were firstly isolated by column chromatography and purified from the extractions of Hydrilla verticillata （Linn. f.） Royle. 1-（5＇-Hydroxy-4＇-hydroxymethyl-1＇-methyl-1H-pyrrol-2＇-yl）-henicosa-2,12,15-trien-1-one （2） and thymidine （3） were identified by EIMS, 1H NMR, laC NMR and IR spectra. Crystal loliolide （1） and sulfur-gamma （4） were identified by X-ray diffraction. Octadecanedioic acid （5） was also elucidated. Among them, compound 2 is a new natural compound, loliolide （1） and thymidine （3） are known bioactive substances. The results provided basic research data for exploring their application as medical materials.展开更多
[Objective] The research aimed to study the influence of temperature on the growth of Hydrilla verticillata in the wastewater treatment process.[Method] By using the single factor experiment design,four temperatures w...[Objective] The research aimed to study the influence of temperature on the growth of Hydrilla verticillata in the wastewater treatment process.[Method] By using the single factor experiment design,four temperatures which were 5,15,25 and 35 ℃ were set.The growth situation of H.verticillata under the different temperatures was observed in the treatment process of domestic sewerage.[Result] H.verticillata grew luxuriantly at 35 and 25 ℃.Via the cultivation of 15 days,the plant length,branch number,root number and biomass of H.verticillata respectively increased 2.8 and 1.2 cm,7.0 and 5.9 branches,6.2 and 3.7 roots,21% and 25%,which were all significantly or extremely significant higher than that at 5 ℃.Under the cultivation condition of 15 ℃,H.verticillata grew slowly.The increment of plant length,branch number and root number were higher than that at 5 ℃,but they had no significant difference.[Conclusion] During 5-35 ℃,the higher temperature could promote the growth of H.verticillata in wastewater treatment process.展开更多
Amiodarone has been widely used since the 1960s for the treatment of troublesome supraventricular and serious ventricular arrhythmias.Amiodarone is an iodine-containing compound that tends to accumulate in several org...Amiodarone has been widely used since the 1960s for the treatment of troublesome supraventricular and serious ventricular arrhythmias.Amiodarone is an iodine-containing compound that tends to accumulate in several organs,including the thyroid gland,skin,lungs,nerves,and cornea.There are several well-established systemic adverse events of amiodarone,including pulmonary toxicity,1 liver cirrhosis,hyperthyroidism,and photodermatitis.2 Of the ocular adverse events,the most common is the appearance of microdeposits in the cornea named cornea verticillata.3 Also,mild lens opacities without visual impairment and optic neuropathy have been reported in patients who had been treated with amiodarone.展开更多
The acute toxicity of Sodium dodecylbenzene sulfonate （SDBS） to subcamersed macrophyte Hydrilla verticillata （L.f） Royle was studied. Chlorophyll contents and the activities of 3 antioxidant enzymes： superoxide d...The acute toxicity of Sodium dodecylbenzene sulfonate （SDBS） to subcamersed macrophyte Hydrilla verticillata （L.f） Royle was studied. Chlorophyll contents and the activities of 3 antioxidant enzymes： superoxide dismutase （SOD, EC 1.15.1.1）, catalase （CAT, EC 1.11.1.6）, peroxidase （POD, EC 1.11.1.7） in the leaves were investigated under different concentrations of SDBS, The chlorophyll contents in leaves of experimental plants decreased gradually, while the electrical conductivity of culture solution increased gradually with the increased dose of SDBS. Under higher concentrations of SDBS （32 mg/L and 128 mg/L） treatment, SOD lost its activities completely after 24 h. The native PAGE analysis indicated the diversity of POD isoenzymes was obvious under different concentrations of SDBS stress.展开更多
[Objective] This study aimed to clone Scrictosidine-β-D-glucosidase (SGD) gene from Rauvolfia verticillata and analyze its characteristics. [Method] The full-length cDNA of SGD was cloned from R. verticillata with RA...[Objective] This study aimed to clone Scrictosidine-β-D-glucosidase (SGD) gene from Rauvolfia verticillata and analyze its characteristics. [Method] The full-length cDNA of SGD was cloned from R. verticillata with RACE technique. Then the expression levels in different tissues were analyzed with quantitative RT-PCR and the bioinformatic characteristics were also predicted. [Result] The full-length cDNA of RvSGD was 1 856 bp, containing a 1 608 bp CDS that encoded 536 amino acids with a calculated molecular mass of 61.0 kDa and an isoelectric point of 6.16. Bioinformatic analysis revealed that RvSGD shared high similarity with SGDs from Cantharanthus roseus and Rauvolfia serpentina at the amino acids; three conserved catalytic sites His-161, Glu-207 and Glu-419 were also presented in RvSGD. Quantitative RT-PCR showed that expression level of RvSGD was the highest in barks, followed by old leaves, roots, tender leaves and tender stems. [Conclusion] The present study helps to understand more about the functions of the SGD gene at the level of molecular genetics, and provides new targets for molecular regulation of TIAs biosynthesis.展开更多
Based on the introduction of characteristics and ecological habits as mesophilia,photophilia,and certain ability of cold resistance of Hydrilla verticillata,the main points of its landscape application,especially on t...Based on the introduction of characteristics and ecological habits as mesophilia,photophilia,and certain ability of cold resistance of Hydrilla verticillata,the main points of its landscape application,especially on the two aspects of its planting and disposing,are discussed in a detailed way.Hydrilla verticillata can be planted in two ways,that is,directly planted into the bottom of the water,or bound with five or six cutting segments and one small piece of stone to sink in the water.On the prospect of disposing,Hydrilla verticillata can be planted together with other submerged plants as well as be planted alone,and also be with ornamental fishes.All these means of disposing would create excellent water landscape effects.Meanwhile the problems in the planting and disposing of Hydrilla verticillata that should be careful with are analyzed with the hope of providing more sufficient and comprehensive basis for the construction of beautiful ecological water landscape.展开更多
Objective: To investigate the effect and mechanism of Rauwolfia verticillata (Lour.) Baill. var. hainanensis Tsiang extract on mice with colitis induced by Dextran sulphate sodium (DSS). Methods: 80 BALB/C mice were r...Objective: To investigate the effect and mechanism of Rauwolfia verticillata (Lour.) Baill. var. hainanensis Tsiang extract on mice with colitis induced by Dextran sulphate sodium (DSS). Methods: 80 BALB/C mice were randomly divided into (1) normal control group, (2) DSS group, (3) DSS + SASP treatment group, (4) DSS + PP treatment group. After the experiment, the mice in each group were observed daily general conditions, Immunohistochemistry was used to detect the expression of ERK, p38 MAPK and JNK protein in colonic mucosa of mice in each group. The expression of TNF-_and IL-6 cytokines in colonic tissue of mice was detected by ELASA. Results: The general situation of SASP group and PP group was better than DSS group;Compared with DSS group, the expression of ERK, p38 MAPK and JNK protein was significantly decreased. The levels of TNF-a and IL-6 in colon tissue of SASP group and PP group were significantly decreased. Conclusion: The results suggest that pectin polysaccharide from Rauvolfia hainanensis extract has a significant therapeutic effect on colitis in DSS mice, which may via regulating the MAPK signal pathway pathways.展开更多
Ammoides verticillata （Desf.） Briq. is an Algerian endemic species; the phytochemical screening （methods of Harborne, 1973） of the aerials parts wealth in polyphenol compounds： flavonoids, saponins, leucoanthocya...Ammoides verticillata （Desf.） Briq. is an Algerian endemic species; the phytochemical screening （methods of Harborne, 1973） of the aerials parts wealth in polyphenol compounds： flavonoids, saponins, leucoanthocyanes, terpens and steroids and tannins; there is no alkaloids. There is an important quantity of essential oils in the flowers; the interest of this study is that this species remains until then it is not very known. The results of Aromatogram method by incorporation of Mtiller-Hinton on solid medium, showed a significant antimicrobial activity （method of Duraffourd, 1987） of the infusion and the ethanolic extract; the infusion of stems and flowers is indeed much more active on Echerichia coli, Citrobacter, Enterobacter, Staph aureus and Staph epidermidis, such as flowers extracts demonstrate an important antimicrobial activity on Staph aureus, Staph epidermidis, Streptococcus, Pseudomonas and Acinetobacter recognized as antibiotic resistant. This could give opportunities for using this species in the treatment of diverse infections and as a disinfecting additive on nosocomial area. The valorization, preservation and sustainable use ofAmmoides verticillata require the protection of its habitats.展开更多
基金National Natural Science Foundation of China(4100117)Major Science and Technology Program for Water Pollution Control and Treatment of China(2008ZX07103-004).
文摘Aims Myriophyllum spicatum and Hydrilla verticillata are common submerged macrophytes in the Yangtze River basin.To investigate their tolerances and adaptations to water-level fluctuations,an experiment was conducted in a pond.Methods We designed five different amplitudes of water-level fluctuations as static,615,630,660 and 690 cm during the 74 days of the experiment.In each amplitude treatment,two cultivation methods were examined as monoculture and mixed culture.Important Findings The results showed that M.spicatum had greater morphological responses to water-level fluctuations than H.verticillata.Fluctuating amplitude had significant effects on branch number,shoot length and root dry weight(DW)of M.spicatum,whereas it only had significant effect on branch number of H.verticillata.Both fluctuating amplitude and cultivation method had significant effects on total DW of M.spicatum,which was higher in monoculture than mixed culture.The total DW of H.verticillata was affected by fluctuating amplitude only,and the largest biomass was in the amplitude of 630 cm.Fruit DW of M.spicatum was largest in the amplitude of 630 cm,high amplitude of water-level fluctuations would inhibit flowering and seed production.Root DW proportion was significantly affected by fluctuating amplitude and cultivation method in both species,and the root DW proportion of M.spicatum was significantly higher in the amplitude-of 690 cm.We conclude that moderate amplitude of water-level fluctuations can promote the distribution and growth of both species,and in order to accelerate the restoration of both species in natural habitats,the optimum amplitude should be keep at 615 to 630 cm.
基金supported financially by Department of Science and Technology, New Delhi, India under the SERC-DST fasttrack scheme.
文摘Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10umol/L Cd, 5 umol/L Hg, and 20 umol/L Cu for 96 h, showed changes in chlorophyll, protein content, and in DNA profiles. The changes in DNA profiles included variation in band intensity, presence or absence of certain bands and even appearance of new bands. Genomic template stability test performed for the qualitative measurement of changes in randomly amplified polymorphic DNA （RAPD） profiles, showed significant effect at the given concentration of metals. Cloning and sequencing of bands suggested that these markers although may not be homologous to any known gene but its conversion as a sequence characterized amplified region （SCAR） marker is useful in detecting the effects of genotoxin agents.
文摘Hydrilla verticillata （Linn. f.） Royle is a submerged plant for phytoremediation on Taihu Lake, China. The planted submerged plant should be harvested in every autumn to reduce the nutrition burden of the lake. Five compounds were firstly isolated by column chromatography and purified from the extractions of Hydrilla verticillata （Linn. f.） Royle. 1-（5＇-Hydroxy-4＇-hydroxymethyl-1＇-methyl-1H-pyrrol-2＇-yl）-henicosa-2,12,15-trien-1-one （2） and thymidine （3） were identified by EIMS, 1H NMR, laC NMR and IR spectra. Crystal loliolide （1） and sulfur-gamma （4） were identified by X-ray diffraction. Octadecanedioic acid （5） was also elucidated. Among them, compound 2 is a new natural compound, loliolide （1） and thymidine （3） are known bioactive substances. The results provided basic research data for exploring their application as medical materials.
基金Supported by The National Natural Science Foundation(30470346)Natural Science Fund of Guangdong Province(06025056)~~
文摘[Objective] The research aimed to study the influence of temperature on the growth of Hydrilla verticillata in the wastewater treatment process.[Method] By using the single factor experiment design,four temperatures which were 5,15,25 and 35 ℃ were set.The growth situation of H.verticillata under the different temperatures was observed in the treatment process of domestic sewerage.[Result] H.verticillata grew luxuriantly at 35 and 25 ℃.Via the cultivation of 15 days,the plant length,branch number,root number and biomass of H.verticillata respectively increased 2.8 and 1.2 cm,7.0 and 5.9 branches,6.2 and 3.7 roots,21% and 25%,which were all significantly or extremely significant higher than that at 5 ℃.Under the cultivation condition of 15 ℃,H.verticillata grew slowly.The increment of plant length,branch number and root number were higher than that at 5 ℃,but they had no significant difference.[Conclusion] During 5-35 ℃,the higher temperature could promote the growth of H.verticillata in wastewater treatment process.
文摘Amiodarone has been widely used since the 1960s for the treatment of troublesome supraventricular and serious ventricular arrhythmias.Amiodarone is an iodine-containing compound that tends to accumulate in several organs,including the thyroid gland,skin,lungs,nerves,and cornea.There are several well-established systemic adverse events of amiodarone,including pulmonary toxicity,1 liver cirrhosis,hyperthyroidism,and photodermatitis.2 Of the ocular adverse events,the most common is the appearance of microdeposits in the cornea named cornea verticillata.3 Also,mild lens opacities without visual impairment and optic neuropathy have been reported in patients who had been treated with amiodarone.
基金the National Natural Science Foundation of China (39925007)the National High Technology Research and Development Program of China (863 Program) (2002AA601021)the Knowledge Innovation Program Key Project of Chinese Academy Sciences (KSCX2-SW-102)
文摘The acute toxicity of Sodium dodecylbenzene sulfonate （SDBS） to subcamersed macrophyte Hydrilla verticillata （L.f） Royle was studied. Chlorophyll contents and the activities of 3 antioxidant enzymes： superoxide dismutase （SOD, EC 1.15.1.1）, catalase （CAT, EC 1.11.1.6）, peroxidase （POD, EC 1.11.1.7） in the leaves were investigated under different concentrations of SDBS, The chlorophyll contents in leaves of experimental plants decreased gradually, while the electrical conductivity of culture solution increased gradually with the increased dose of SDBS. Under higher concentrations of SDBS （32 mg/L and 128 mg/L） treatment, SOD lost its activities completely after 24 h. The native PAGE analysis indicated the diversity of POD isoenzymes was obvious under different concentrations of SDBS stress.
文摘[Objective] This study aimed to clone Scrictosidine-β-D-glucosidase (SGD) gene from Rauvolfia verticillata and analyze its characteristics. [Method] The full-length cDNA of SGD was cloned from R. verticillata with RACE technique. Then the expression levels in different tissues were analyzed with quantitative RT-PCR and the bioinformatic characteristics were also predicted. [Result] The full-length cDNA of RvSGD was 1 856 bp, containing a 1 608 bp CDS that encoded 536 amino acids with a calculated molecular mass of 61.0 kDa and an isoelectric point of 6.16. Bioinformatic analysis revealed that RvSGD shared high similarity with SGDs from Cantharanthus roseus and Rauvolfia serpentina at the amino acids; three conserved catalytic sites His-161, Glu-207 and Glu-419 were also presented in RvSGD. Quantitative RT-PCR showed that expression level of RvSGD was the highest in barks, followed by old leaves, roots, tender leaves and tender stems. [Conclusion] The present study helps to understand more about the functions of the SGD gene at the level of molecular genetics, and provides new targets for molecular regulation of TIAs biosynthesis.
文摘Based on the introduction of characteristics and ecological habits as mesophilia,photophilia,and certain ability of cold resistance of Hydrilla verticillata,the main points of its landscape application,especially on the two aspects of its planting and disposing,are discussed in a detailed way.Hydrilla verticillata can be planted in two ways,that is,directly planted into the bottom of the water,or bound with five or six cutting segments and one small piece of stone to sink in the water.On the prospect of disposing,Hydrilla verticillata can be planted together with other submerged plants as well as be planted alone,and also be with ornamental fishes.All these means of disposing would create excellent water landscape effects.Meanwhile the problems in the planting and disposing of Hydrilla verticillata that should be careful with are analyzed with the hope of providing more sufficient and comprehensive basis for the construction of beautiful ecological water landscape.
基金National Natural Science Foundation of China(No.81360603)Natural Science Foundation of Hainan Province(No.813215).
文摘Objective: To investigate the effect and mechanism of Rauwolfia verticillata (Lour.) Baill. var. hainanensis Tsiang extract on mice with colitis induced by Dextran sulphate sodium (DSS). Methods: 80 BALB/C mice were randomly divided into (1) normal control group, (2) DSS group, (3) DSS + SASP treatment group, (4) DSS + PP treatment group. After the experiment, the mice in each group were observed daily general conditions, Immunohistochemistry was used to detect the expression of ERK, p38 MAPK and JNK protein in colonic mucosa of mice in each group. The expression of TNF-_and IL-6 cytokines in colonic tissue of mice was detected by ELASA. Results: The general situation of SASP group and PP group was better than DSS group;Compared with DSS group, the expression of ERK, p38 MAPK and JNK protein was significantly decreased. The levels of TNF-a and IL-6 in colon tissue of SASP group and PP group were significantly decreased. Conclusion: The results suggest that pectin polysaccharide from Rauvolfia hainanensis extract has a significant therapeutic effect on colitis in DSS mice, which may via regulating the MAPK signal pathway pathways.
文摘Ammoides verticillata （Desf.） Briq. is an Algerian endemic species; the phytochemical screening （methods of Harborne, 1973） of the aerials parts wealth in polyphenol compounds： flavonoids, saponins, leucoanthocyanes, terpens and steroids and tannins; there is no alkaloids. There is an important quantity of essential oils in the flowers; the interest of this study is that this species remains until then it is not very known. The results of Aromatogram method by incorporation of Mtiller-Hinton on solid medium, showed a significant antimicrobial activity （method of Duraffourd, 1987） of the infusion and the ethanolic extract; the infusion of stems and flowers is indeed much more active on Echerichia coli, Citrobacter, Enterobacter, Staph aureus and Staph epidermidis, such as flowers extracts demonstrate an important antimicrobial activity on Staph aureus, Staph epidermidis, Streptococcus, Pseudomonas and Acinetobacter recognized as antibiotic resistant. This could give opportunities for using this species in the treatment of diverse infections and as a disinfecting additive on nosocomial area. The valorization, preservation and sustainable use ofAmmoides verticillata require the protection of its habitats.
