在不同的钒（V）胁迫浓度（0、25、50、100和200 mg＆#183; kg -1）条件下，通过40 d盆栽试验，研究了枸杞植株对V积累及V对枸杞叶中细胞膜透性、抗氧化酶活性，以及丙二醛（ MDA）、脯氨酸（ Pro）、叶绿素和可溶性蛋白含量的影响。结...在不同的钒（V）胁迫浓度（0、25、50、100和200 mg＆#183; kg -1）条件下，通过40 d盆栽试验，研究了枸杞植株对V积累及V对枸杞叶中细胞膜透性、抗氧化酶活性，以及丙二醛（ MDA）、脯氨酸（ Pro）、叶绿素和可溶性蛋白含量的影响。结果表明，枸杞中各部位V含量分布大小的顺序依次为根＞叶＞茎。随着V浓度增大，过氧化氢酶（CAT）和过氧化物酶（POD）活性、叶绿素含量和可溶性蛋白含量等呈先上升、后下降的变化趋势，当 V 胁迫浓度≤100 mg ＆#183; kg -1时，与对照组相比， CAT和POD的活性、叶绿素含量和可溶性蛋白含量分别增大了227.9％、76.2％、40.2％和117.4％；当V胁迫浓度＞100 mg＆#183; kg -1，则分别降低了5.1％、5.8％、22.8％和36.7％。在不同浓度V的处理下， MDA含量、细胞膜透性和 Pro 含量比对照分别增加了13.3％～220.7％、12.4％～76.9％和69.7％～276.3％。可见，低浓度V （＜100 mg＆#183; kg -1）刺激了叶绿素和抗氧化酶活性的增加，促进了植物可溶性蛋白质的合成；高浓度V （≥100 mg ＆#183; kg-1）破坏了叶绿素结构，抑制了抗氧化酶和可溶性蛋白的合成，膜脂过氧化作用和细胞膜透性显著增大，游离脯氨酸含量明显增加。展开更多
The objective of this paper was to evaluate the effect of vanadium(V)in high-fat diets sourced from egg yolk on body weight gain,feed intake,blood characteristics and antioxidative status of Wistar rats.A total of 72 ...The objective of this paper was to evaluate the effect of vanadium(V)in high-fat diets sourced from egg yolk on body weight gain,feed intake,blood characteristics and antioxidative status of Wistar rats.A total of 72 female Wistar rats were allocated according to a 2 x 4 factorial design throughout a 5-wk trial,including 2 levels of dietary fat(normal and high;ether extract 40.3 and 301.2 g/kg;fat sourced from egg yolk)and 4 levels of dietary V(0,3,15 and 30 mg/kg).Vanadium decreased(P<0.05)body weight gain(V at 30 mg/kg during wk 1 and 2;V at 15 and 30 mg/kg during the overall phase),feed intake(V at30 mg/kg during wk 3 and the overall phase;V at 15 and 30 mg/kg during wk 4),but increased the relative weight of liver(V at 30 mg/kg,P<0.05).Moreover,increasing dietary V significantly increased(P<0.05)plasma aspartate aminotransferase,alanine aminotransferase and malondialdehyde levels and decreased triglyceride level,and V at 30 mg/kg in high-fat treatment had the highest or lowest values(interaction,P<0.05).Under the same dietary V dose,V residual content in liver(dietary V at 15 and30 mg/kg)and kidney(dietary V at 15 mg/kg)was higher in high-fat diet treatment compared with normal-fat diet treatment(P<0.05).In conclusion,it is suggested that V could decrease the body weight together with the feed intake,and the high fat could enhance oxidative stress induced by V of Wistar rats.展开更多
Single-pass compression tests were performed to investigate the hot deformation behavior of low-carbon boron microalloyed steel containing three various vanadium contents at 900-1100℃and strain rate of 0.01-10 s-1 us...Single-pass compression tests were performed to investigate the hot deformation behavior of low-carbon boron microalloyed steel containing three various vanadium contents at 900-1100℃and strain rate of 0.01-10 s-1 using the MMS-300 thermal mechanical simulator.The flow stress curves of investigated steels were obtained under the different deformation conditions,and the effects of the deformation temperature and strain rate on the flow stress were discussed.The characteristic points of flow stress were obtained from the stress dependence of strain hardening rate;the activation energy of investigated steels was determined by the regression analysis;the flow stress constitutive equations were developed;the effect of vanadium content on the flow stress and dynamic recrystallization(DRX)was investigated.The result showed that the flow stress and activation energy(3-6.5 kJ mol-1)of the steel containing 0.18 wt%V were significantly higher than those of the steels with0.042 wt%and 0.086 wt%V,which was related to the increase in solute drag and precipitation effects for higher vanadium content.DRX analysis showed that the addition of vanadium can delay the initiation and the rate of DRX.展开更多
文摘在不同的钒（V）胁迫浓度（0、25、50、100和200 mg＆#183; kg -1）条件下，通过40 d盆栽试验，研究了枸杞植株对V积累及V对枸杞叶中细胞膜透性、抗氧化酶活性，以及丙二醛（ MDA）、脯氨酸（ Pro）、叶绿素和可溶性蛋白含量的影响。结果表明，枸杞中各部位V含量分布大小的顺序依次为根＞叶＞茎。随着V浓度增大，过氧化氢酶（CAT）和过氧化物酶（POD）活性、叶绿素含量和可溶性蛋白含量等呈先上升、后下降的变化趋势，当 V 胁迫浓度≤100 mg ＆#183; kg -1时，与对照组相比， CAT和POD的活性、叶绿素含量和可溶性蛋白含量分别增大了227.9％、76.2％、40.2％和117.4％；当V胁迫浓度＞100 mg＆#183; kg -1，则分别降低了5.1％、5.8％、22.8％和36.7％。在不同浓度V的处理下， MDA含量、细胞膜透性和 Pro 含量比对照分别增加了13.3％～220.7％、12.4％～76.9％和69.7％～276.3％。可见，低浓度V （＜100 mg＆#183; kg -1）刺激了叶绿素和抗氧化酶活性的增加，促进了植物可溶性蛋白质的合成；高浓度V （≥100 mg ＆#183; kg-1）破坏了叶绿素结构，抑制了抗氧化酶和可溶性蛋白的合成，膜脂过氧化作用和细胞膜透性显著增大，游离脯氨酸含量明显增加。
基金fanatically supported by Ministry of Science and Technology Support Program(2014BAD13B04)National Natural Science Foundation of China(31402031)+1 种基金Department of Education Project of Sichuan Province(13ZB0290)Department of Science and Technology Project of Sichuan Province(2014NZ0043,2014NZ0002,2013NZ0054)
文摘The objective of this paper was to evaluate the effect of vanadium(V)in high-fat diets sourced from egg yolk on body weight gain,feed intake,blood characteristics and antioxidative status of Wistar rats.A total of 72 female Wistar rats were allocated according to a 2 x 4 factorial design throughout a 5-wk trial,including 2 levels of dietary fat(normal and high;ether extract 40.3 and 301.2 g/kg;fat sourced from egg yolk)and 4 levels of dietary V(0,3,15 and 30 mg/kg).Vanadium decreased(P<0.05)body weight gain(V at 30 mg/kg during wk 1 and 2;V at 15 and 30 mg/kg during the overall phase),feed intake(V at30 mg/kg during wk 3 and the overall phase;V at 15 and 30 mg/kg during wk 4),but increased the relative weight of liver(V at 30 mg/kg,P<0.05).Moreover,increasing dietary V significantly increased(P<0.05)plasma aspartate aminotransferase,alanine aminotransferase and malondialdehyde levels and decreased triglyceride level,and V at 30 mg/kg in high-fat treatment had the highest or lowest values(interaction,P<0.05).Under the same dietary V dose,V residual content in liver(dietary V at 15 and30 mg/kg)and kidney(dietary V at 15 mg/kg)was higher in high-fat diet treatment compared with normal-fat diet treatment(P<0.05).In conclusion,it is suggested that V could decrease the body weight together with the feed intake,and the high fat could enhance oxidative stress induced by V of Wistar rats.
基金supported by the Kim Chaek University of Technology of DPR of Koreathe State Key Laboratory of Rolling and Automation of Northeastern University of China
文摘Single-pass compression tests were performed to investigate the hot deformation behavior of low-carbon boron microalloyed steel containing three various vanadium contents at 900-1100℃and strain rate of 0.01-10 s-1 using the MMS-300 thermal mechanical simulator.The flow stress curves of investigated steels were obtained under the different deformation conditions,and the effects of the deformation temperature and strain rate on the flow stress were discussed.The characteristic points of flow stress were obtained from the stress dependence of strain hardening rate;the activation energy of investigated steels was determined by the regression analysis;the flow stress constitutive equations were developed;the effect of vanadium content on the flow stress and dynamic recrystallization(DRX)was investigated.The result showed that the flow stress and activation energy(3-6.5 kJ mol-1)of the steel containing 0.18 wt%V were significantly higher than those of the steels with0.042 wt%and 0.086 wt%V,which was related to the increase in solute drag and precipitation effects for higher vanadium content.DRX analysis showed that the addition of vanadium can delay the initiation and the rate of DRX.
