This research proposes a new theory in the field of physics and space technology, based on several scientific hypotheses and conclusions, as it plans to manufacture a “new magnetic focus” by means of high-speed cent...This research proposes a new theory in the field of physics and space technology, based on several scientific hypotheses and conclusions, as it plans to manufacture a “new magnetic focus” by means of high-speed centrifugal engines, by equipping a group of them, and installing heavy, dense fans, not for the purpose of air pressure, Rather, it works to rotate at a speed of up to 1000 kilometers per hour at an angle of 45 degrees, and is lined up in front of each other in a circle, so that the final result is to transfer the effort and weight of the propellers to an upper point in their midst, and this point will reach a large degree of density, size and mass, causing the generation of a point independent magnetic attraction, which pulls the motors upward, as a result of the mutual gravitational effect created by the motors. These hypotheses need a large number of experiments, applications, and analyze the use of specialists in physics, technology, technology, engine engineers, and others, and the correct results will cause a major revolution in the field of space technology, and this theory will be relied upon in many means of land, air, and space transportation and different fields.展开更多
文摘目的 比较超高速差速离心法和试剂盒法提取肺动脉平滑肌细胞(PASMCs)源性外泌体的纯度,并评价超高速差速离心法提取外泌体的稳定性和适用性。探究低氧肺动脉高压(PH)大鼠血浆源性外泌体对PASMCs表型转化和肺动脉内皮细胞(PAECs)内皮间质转化的作用。方法 ① 收集PASMCs培养基上清液,分别用超高速差速离心法和试剂盒法提取外泌体,通过透射电子显微镜观察外泌体形态、纳米微粒示踪分析检测外泌体粒径分布、蛋白质免疫印迹法检测外泌体标志蛋白表达,评估外泌体纯度;② 收集PAECs培养基上清液和大鼠血浆,超高速差速离心法分别提取外泌体并评估外泌体纯度;③ 构建低氧PH大鼠模型,超高速差速离心法分别提取常氧和低氧大鼠血浆源性外泌体并作用于PASMCs和PAECs,免疫荧光检测各标志蛋白表达以评估外泌体对PASMCs表型转化和PAECs内皮间质转化的影响。结果 ① 超高速差速离心法提取的PASMCs、PAECs和血浆源性外泌体形态典型,纯度较高;而试剂盒法提取的PASMCs源性外泌体纯度较低。② 低氧PH大鼠血浆源性外泌体诱导PASMCs发生表型转化和PAECs发生内皮间质转化。结论 超高速差速离心法提取外泌体纯度优于试剂盒法,实验方法稳定且适用性强。低氧PH大鼠血浆源性外泌体可诱导PH肺血管重构。
文摘This research proposes a new theory in the field of physics and space technology, based on several scientific hypotheses and conclusions, as it plans to manufacture a “new magnetic focus” by means of high-speed centrifugal engines, by equipping a group of them, and installing heavy, dense fans, not for the purpose of air pressure, Rather, it works to rotate at a speed of up to 1000 kilometers per hour at an angle of 45 degrees, and is lined up in front of each other in a circle, so that the final result is to transfer the effort and weight of the propellers to an upper point in their midst, and this point will reach a large degree of density, size and mass, causing the generation of a point independent magnetic attraction, which pulls the motors upward, as a result of the mutual gravitational effect created by the motors. These hypotheses need a large number of experiments, applications, and analyze the use of specialists in physics, technology, technology, engine engineers, and others, and the correct results will cause a major revolution in the field of space technology, and this theory will be relied upon in many means of land, air, and space transportation and different fields.
