The application of medical devices to repair skin damage is clinically accepted and natural polymer enjoys an important role in this field,such as collagen or hyaluronic acid,etc.However,the biosafety and efficacy of ...The application of medical devices to repair skin damage is clinically accepted and natural polymer enjoys an important role in this field,such as collagen or hyaluronic acid,etc.However,the biosafety and efficacy of these implants are still challenged.In this study,a skin damage animal model was prepared by UV-photoaging and recombinant humanized type Ⅲ collagen(rhCol Ⅲ)was applied as a bioactive material to implant in vivo to study its biological effect,comparing with saline and uncrosslinked hyaluronic acid(HA).Animal skin conditions were non-invasively and dynamically monitored during the 8 weeks experiment.Histological observation,specific gene expression and other molecular biological methods were applied by the end of the animal experiment.The results indicated that rhCol Ⅲ could alleviate the skin photoaging caused by UV radiation,including reduce the thickening of epidermis and dermis,increase the secretion of Collagen Ⅰ(Col Ⅰ)and Collagen Ⅲ(Col Ⅲ)and remodel of extracellular matrix(ECM).Although the cell-material interaction and mechanism need more investigation,the effect of rhCol Ⅲ on damaged skin was discussed from influence on cells,reconstruction of ECM,and stimulus of small biological molecules based on current results.In conclusion,our findings provided rigorous biosafety information of rhCol Ⅲ and approved its potential in skin repair and regeneration.Although enormous efforts still need to be made to achieve successful translation from bench to clinic,the recombinant humanized collagen showed superiorities from both safety and efficacy aspects.展开更多
目的:研究抗纤灵方对5/6肾切除小鼠肾纤维化的作用及机制研究。方法:将60只C57小鼠,随机分为假手术组10只和手术组50只,手术组行5/6肾切除术。术后2周,手术组随机分为模型组、抗纤灵低、中、高剂量组(0.1,0.2,0.4 mg·kg^(-1))及雷...目的:研究抗纤灵方对5/6肾切除小鼠肾纤维化的作用及机制研究。方法:将60只C57小鼠,随机分为假手术组10只和手术组50只,手术组行5/6肾切除术。术后2周,手术组随机分为模型组、抗纤灵低、中、高剂量组(0.1,0.2,0.4 mg·kg^(-1))及雷帕霉素组(0.16μg·kg^(-1)),各组10只。各组于术后2周分别给予0.5 mL生理盐水、雷帕霉素及抗纤灵煎剂灌胃。治疗8周后处死小鼠,在处死小鼠前1 d收集24 h小便测24 h尿蛋白(UPRO)定量,眼眶采血测血肌酐(SCr),尿素氮(BUN);取残肾应用免疫荧光法检测肾组织α-平滑肌肌动蛋白(α-SMA)表达;系用Western blot法检测肾组织Ⅰ型及Ⅲ型胶原蛋白表达。结果:与假手术组比较,模型组24 h UPRO定量,SCr,BUN,α-SMA,Ⅰ型及Ⅲ型胶原表达显著升高(P<0.01);与模型组比较,各治疗组24 h UPRO定量,SCr,BUN,α-SMA,Ⅰ型及Ⅲ型胶原表达均有所下降(P<0.05)。结论:抗纤灵方能降低小鼠24 h UPRO定量,改善肾功能,延缓肾纤维化发生,其机制可能与下调α-SMA表达,抑制肾脏Ⅰ型及Ⅲ型胶原表达相关。展开更多
基金financially supported by the National Key Research and Development Program of China(2018YFC1106200 and 2018YFC1106203)the National Natural Science Foundation of China(32071330).
文摘The application of medical devices to repair skin damage is clinically accepted and natural polymer enjoys an important role in this field,such as collagen or hyaluronic acid,etc.However,the biosafety and efficacy of these implants are still challenged.In this study,a skin damage animal model was prepared by UV-photoaging and recombinant humanized type Ⅲ collagen(rhCol Ⅲ)was applied as a bioactive material to implant in vivo to study its biological effect,comparing with saline and uncrosslinked hyaluronic acid(HA).Animal skin conditions were non-invasively and dynamically monitored during the 8 weeks experiment.Histological observation,specific gene expression and other molecular biological methods were applied by the end of the animal experiment.The results indicated that rhCol Ⅲ could alleviate the skin photoaging caused by UV radiation,including reduce the thickening of epidermis and dermis,increase the secretion of Collagen Ⅰ(Col Ⅰ)and Collagen Ⅲ(Col Ⅲ)and remodel of extracellular matrix(ECM).Although the cell-material interaction and mechanism need more investigation,the effect of rhCol Ⅲ on damaged skin was discussed from influence on cells,reconstruction of ECM,and stimulus of small biological molecules based on current results.In conclusion,our findings provided rigorous biosafety information of rhCol Ⅲ and approved its potential in skin repair and regeneration.Although enormous efforts still need to be made to achieve successful translation from bench to clinic,the recombinant humanized collagen showed superiorities from both safety and efficacy aspects.
文摘目的:研究抗纤灵方对5/6肾切除小鼠肾纤维化的作用及机制研究。方法:将60只C57小鼠,随机分为假手术组10只和手术组50只,手术组行5/6肾切除术。术后2周,手术组随机分为模型组、抗纤灵低、中、高剂量组(0.1,0.2,0.4 mg·kg^(-1))及雷帕霉素组(0.16μg·kg^(-1)),各组10只。各组于术后2周分别给予0.5 mL生理盐水、雷帕霉素及抗纤灵煎剂灌胃。治疗8周后处死小鼠,在处死小鼠前1 d收集24 h小便测24 h尿蛋白(UPRO)定量,眼眶采血测血肌酐(SCr),尿素氮(BUN);取残肾应用免疫荧光法检测肾组织α-平滑肌肌动蛋白(α-SMA)表达;系用Western blot法检测肾组织Ⅰ型及Ⅲ型胶原蛋白表达。结果:与假手术组比较,模型组24 h UPRO定量,SCr,BUN,α-SMA,Ⅰ型及Ⅲ型胶原表达显著升高(P<0.01);与模型组比较,各治疗组24 h UPRO定量,SCr,BUN,α-SMA,Ⅰ型及Ⅲ型胶原表达均有所下降(P<0.05)。结论:抗纤灵方能降低小鼠24 h UPRO定量,改善肾功能,延缓肾纤维化发生,其机制可能与下调α-SMA表达,抑制肾脏Ⅰ型及Ⅲ型胶原表达相关。