The spectroscopic properties of a new chlorophyll derivate photosensitizer (CDP) are studied under the excitation wavelengths at 800 and 400 nm using femtosecond pulses from a Ti: sapphire laser. The damaging effect o...The spectroscopic properties of a new chlorophyll derivate photosensitizer (CDP) are studied under the excitation wavelengths at 800 and 400 nm using femtosecond pulses from a Ti: sapphire laser. The damaging effect of CDP on the BEL-7402 cancer cells is also investigated upon two-photon illumination at 800 nm. The normalized fluorescence spectra of CDP in tetrahydrofuran (THF) show that two-photon and one-photon spectra have the same distributions and the same emission bands (675 nm). The lifetimes of two- and one-photon induced fluorescence of this molecule are of the order of 5.0 ns. By comparing the data it is shown that there is some difference between the two lifetimes, but the difference is less than one nanosecond. The two-photon absorption cross section of the molecule is also measured at 800 nm and estimated as about σ'_2 ≈ 31.5x10^(-50)cm^4·s·photon^(-1). The results of two-photon photodynamic therapy (TPPDT) tests show that CDP can kill all of the tested cancer cells according to the usual Eosine assessment. Our results indicate that the two-photon-induced photophysical, photochemical and photosensitizing processes of CDP may be basically similar to those of one-photon excitation. These behaviors of the sample suggest that one may find other possible methods to estimate some photosensitizers' effects in details such as their distribution in cells and the reactive targets of the sub-cellular parts of some tumor cells via two-photon excitation techniques.展开更多
Different types of stilbene derivatives (D-p-D, A-p-A, D-p-A) were investigated with AM1, and specially, equilibrium geometries of symmetrical stilbene derivatives (D-p-D) were studied using of PM3. With the same meth...Different types of stilbene derivatives (D-p-D, A-p-A, D-p-A) were investigated with AM1, and specially, equilibrium geometries of symmetrical stilbene derivatives (D-p-D) were studied using of PM3. With the same method INDO/CI, the UV-vis spectra were explored and the position and strength of the two-photon absorption were predicated by Sum-Over-States expression. The relationships of the structures, spectra and nonlinear optical properties have been examined. The influence of various substituents on two photon absorption cross-sections was discussed micromechanically.展开更多
合成了一种新型三取代结构的双光子聚合引发剂1,3,5-三[4-(N,N-二乙氨基)苯基]乙烯基]苯基]苯(4),通过元素分析、电喷雾质谱、1 H NMR和红外谱对其进行表征。测试了紫外吸收光谱、单光子荧光光谱和双光子荧光光谱,并测出其双光子吸收截...合成了一种新型三取代结构的双光子聚合引发剂1,3,5-三[4-(N,N-二乙氨基)苯基]乙烯基]苯基]苯(4),通过元素分析、电喷雾质谱、1 H NMR和红外谱对其进行表征。测试了紫外吸收光谱、单光子荧光光谱和双光子荧光光谱,并测出其双光子吸收截面为9.87×10-48cm4.s/photon。利用合成的1,3,5-三[4-(N,N-二乙氨基)苯基]乙烯基]苯基]苯作双光子聚合引发剂,以甲基丙烯酸甲酯齐聚物作单体(CN120C80),200fs,76MHz、Ti:sapphire飞秒激光器作光源,进行了三维周期微结构的制作。所合成化合物是潜在应用前景的双光子吸收材料。展开更多
基金the Science and Technology Committee of Tianjin,China(Grant No. 023107611)the Key Subject Construction Project of Hebei Provincial Universitythe Creative Funds of Nankai University and the Fund of Hebei University of Technology
文摘The spectroscopic properties of a new chlorophyll derivate photosensitizer (CDP) are studied under the excitation wavelengths at 800 and 400 nm using femtosecond pulses from a Ti: sapphire laser. The damaging effect of CDP on the BEL-7402 cancer cells is also investigated upon two-photon illumination at 800 nm. The normalized fluorescence spectra of CDP in tetrahydrofuran (THF) show that two-photon and one-photon spectra have the same distributions and the same emission bands (675 nm). The lifetimes of two- and one-photon induced fluorescence of this molecule are of the order of 5.0 ns. By comparing the data it is shown that there is some difference between the two lifetimes, but the difference is less than one nanosecond. The two-photon absorption cross section of the molecule is also measured at 800 nm and estimated as about σ'_2 ≈ 31.5x10^(-50)cm^4·s·photon^(-1). The results of two-photon photodynamic therapy (TPPDT) tests show that CDP can kill all of the tested cancer cells according to the usual Eosine assessment. Our results indicate that the two-photon-induced photophysical, photochemical and photosensitizing processes of CDP may be basically similar to those of one-photon excitation. These behaviors of the sample suggest that one may find other possible methods to estimate some photosensitizers' effects in details such as their distribution in cells and the reactive targets of the sub-cellular parts of some tumor cells via two-photon excitation techniques.
基金Project supported by the National Natural Science Foundation of China (Nos. 20273023 90101026) and the Key Laboratory for Supramolecular Structure and Material of Jilin University.
文摘Different types of stilbene derivatives (D-p-D, A-p-A, D-p-A) were investigated with AM1, and specially, equilibrium geometries of symmetrical stilbene derivatives (D-p-D) were studied using of PM3. With the same method INDO/CI, the UV-vis spectra were explored and the position and strength of the two-photon absorption were predicated by Sum-Over-States expression. The relationships of the structures, spectra and nonlinear optical properties have been examined. The influence of various substituents on two photon absorption cross-sections was discussed micromechanically.
文摘合成了一种新型三取代结构的双光子聚合引发剂1,3,5-三[4-(N,N-二乙氨基)苯基]乙烯基]苯基]苯(4),通过元素分析、电喷雾质谱、1 H NMR和红外谱对其进行表征。测试了紫外吸收光谱、单光子荧光光谱和双光子荧光光谱,并测出其双光子吸收截面为9.87×10-48cm4.s/photon。利用合成的1,3,5-三[4-(N,N-二乙氨基)苯基]乙烯基]苯基]苯作双光子聚合引发剂,以甲基丙烯酸甲酯齐聚物作单体(CN120C80),200fs,76MHz、Ti:sapphire飞秒激光器作光源,进行了三维周期微结构的制作。所合成化合物是潜在应用前景的双光子吸收材料。
