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Two-dimensional silicon nanomaterials for optoelectronics
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作者 Xuebiao Deng Huai Chen Zhenyu Yang 《Journal of Semiconductors》 EI CAS CSCD 2023年第4期15-29,共15页
Silicon nanomaterials have been of immense interest in the last few decades due to their remarkable optoelectronic responses,elemental abundance,and higher biocompatibility.Two-dimensional silicon is one of the new al... Silicon nanomaterials have been of immense interest in the last few decades due to their remarkable optoelectronic responses,elemental abundance,and higher biocompatibility.Two-dimensional silicon is one of the new allotropes of silicon and has many compelling properties such as quantum-confined photoluminescence,high charge carrier mobilities,anisotropic electronic and magnetic response,and non-linear optical properties.This review summarizes the recent advances in the synthesis of two-dimensional silicon nanomaterials with a range of structures(silicene,silicane,and multilayered silicon),surface ligand engineering,and corresponding optoelectronic applications. 展开更多
关键词 two-dimensionality SILICON NANOMATERIALS SYNTHESIS surface engineering OPTOELECTRONICS
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Low Rm MHD Turbulence:The Role of Boundaries
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作者 Alban Pothérat 《Journal of Iron and Steel Research International》 SCIE EI CAS CSCD 2012年第S1期514-518,共5页
In this Keynote lecture,we review the main known features of MHD turbulence at low magnetic Reynolds number,that is when the flow isn't intense enough to disturb an externally applied magnetic field.The emphasis i... In this Keynote lecture,we review the main known features of MHD turbulence at low magnetic Reynolds number,that is when the flow isn't intense enough to disturb an externally applied magnetic field.The emphasis is deliberately placed on the very specific physical mechanisms of this type of flows,rather than their numerical modelling. We also focus on homogeneous magnetic fields which have received most attention.We stress in particular that the tendency to two-dimensionality observed in these flows casts the boundaries of the domain into a leading role. 展开更多
关键词 magnetic field MHD turbulence Lorentz force two-dimensionality THREE-dimensionality
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高负荷扩压叶栅吹风试验流场二维性控制技术研究 被引量:1
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作者 高丽敏 刘哲 +1 位作者 蔡明 黎浩学 《实验流体力学》 CAS CSCD 北大核心 2021年第2期13-21,共9页
平面叶栅风洞侧壁附面层引起流道收缩,破坏叶栅流场二维性,扩压叶栅逆压梯度会加剧收缩,且随负荷增加越发显著。针对某高负荷扩压叶栅,研究了影响叶栅吹风试验二维性的因素及不同轴向位置端壁抽吸的改善效果并探索了分布式抽吸。结果表... 平面叶栅风洞侧壁附面层引起流道收缩,破坏叶栅流场二维性,扩压叶栅逆压梯度会加剧收缩,且随负荷增加越发显著。针对某高负荷扩压叶栅,研究了影响叶栅吹风试验二维性的因素及不同轴向位置端壁抽吸的改善效果并探索了分布式抽吸。结果表明:常规试验叶栅端壁附面层发展会挤压主流,使其加速,扩压性下降,造成流场失真,总压损失偏差最小达23%。前部、中部抽吸可整体控制叶栅二维性,但展向二维区较窄;尾部抽吸出口展向二维区较宽,但仅局部改善近尾缘处二维性。前部抽吸在全攻角下控制良好,中部抽吸的负攻角特性较好,尾部抽吸流量则随攻角呈线性变化。分布式抽吸能整体控制二维性同时拓宽展向二维区,值得探索与应用。 展开更多
关键词 高负荷扩压叶栅 吹风试验 二维性 端壁抽吸 控制技术
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三维CAD在减速顶设计中的开发与应用
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作者 钮艳华 《减速顶与调速技术》 2000年第3期11-13,共3页
本文对三维 CAD技术进行了简单介绍 ,同时针对减速顶设计对软件的二次开发进行了讨论。
关键词 减速顶 参数化 设计 三维CAD 软件开发 应用 设计
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社会认同的内在二维图式——以北京市农民工的社会认同研究为例 被引量:18
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作者 郭星华 邢朝国 《江苏社会科学》 CSSCI 北大核心 2009年第4期54-60,共7页
本文提出社会认同内在二维性这一概念,认为社会认同不是同质线性的,而是在两个维度上发生变化,即对社会群体本身的认知与评价以及对自身与社会群体之间关系的认知与评价。这两个维度并不一定同时变化,且变化方向也不一定相同。文章认为... 本文提出社会认同内在二维性这一概念,认为社会认同不是同质线性的,而是在两个维度上发生变化,即对社会群体本身的认知与评价以及对自身与社会群体之间关系的认知与评价。这两个维度并不一定同时变化,且变化方向也不一定相同。文章认为社会认同的这种内在二维性是导致个体社会认同的张力和矛盾性的重要原因。在此基础上,文章对北京市农民工的社会认同状况进行分析,尝试勾勒农民工社会认同的图式,并以此说明社会认同的内在二维性问题。结果显示农民工的社会认同并不是单维度线性的,而是具有多样化的发展方向,并呈现出多元化的图景。 展开更多
关键词 社会认同 内在二维性 北京市农民工
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身份认同的内在二维图式——以鄂西北HL村艾滋病人的身份认同研究为例 被引量:1
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作者 陈静 张建 《社会工作》 2012年第5期85-88,共4页
根据身份认同的定义,个体身份认同具有内在二维属性,即对身份角色的确认和对所属群体的情感归属。而这两个维度并不一定是同时发生,也不一定向同方向发生转化。而身份认同的内在二维性是导致个体身份认同的张力和矛盾的重要原因。在此... 根据身份认同的定义,个体身份认同具有内在二维属性,即对身份角色的确认和对所属群体的情感归属。而这两个维度并不一定是同时发生,也不一定向同方向发生转化。而身份认同的内在二维性是导致个体身份认同的张力和矛盾的重要原因。在此基础上,本文对XY市HL村的艾滋病人身份认同进行分析,尝试勾勒出作为特殊群体的艾滋病人身份认同的图示,说明身份认同的非单线和非单向性,个体身份认同的建构是内在主体行动和社会外在力量共同作用的结果。 展开更多
关键词 身份认同 内在二维性 艾滋病人
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海岸河口潮流数值模拟的研究与进展 被引量:64
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作者 李孟国 曹祖德 《海洋学报》 CAS CSCD 北大核心 1999年第1期111-125,共15页
对海岸河口地区潮流场的数值模拟方法进行了较为系统的归纳总结和评述,对潮流数值模拟的发展趋势进行了分析.
关键词 海岸河口 潮流 数值模拟 潮汐
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Validation of real-time three-dimensional echocardiography for quantifying left and right ventricular volumes:an experimental study 被引量:38
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作者 费洪文 王新房 +5 位作者 谢明星 庄磊 陈立新 杨颖 黄润青 王静 《Chinese Medical Journal》 SCIE CAS CSCD 2004年第5期695-699,共5页
Background Assessment of the left ventricular (LV) and the right ventricular (RV) volumes and their functions is important for prognostic prediction and clinical decision making. We compared the accuracy for quantify... Background Assessment of the left ventricular (LV) and the right ventricular (RV) volumes and their functions is important for prognostic prediction and clinical decision making. We compared the accuracy for quantifying the LV and the RV volumes in vitro between conventional two-dimensional echocardiography (2DE) and real-time three-dimensional echocardiography (RT3DE) Methods The volumes of 37 rubber-models (10 regularly shaped to simulate normal LV, 7 shaped to simulate LV with symmetric aneurysm, 8 shaped to simulate LV with asymmetric aneurysm, and 12 irregularly shaped to simulate normal RV) and 10 excised canine hearts were measured by RT3DE and 2DE On RT3DE 'full volume' imaging, the inner-surfaces of the rubber-models and canine LV and RV were outlined and the volumes were measured using 2-, 4-, 8- and 16-plane methods with the RT3DE analysis software On 2DE imaging, the volumes were measured by the Simpson method The LV and RV volumes measured by drained water were served as reference values, with which we compared RT3DE and 2DE data Results In rubber models mimicking normal LV and LV with symmetric aneurysms, RT3DE results were strongly correlated with reference values ( r =0 795-0 998) and there was a good correlation between 2DE estimates and reference values ( r =0 715-0 729) There were no significant differences between RT3DE estimates, 2DE results and reference values ( P >0 05) In rubber models mimicking the RV and LV with asymmetric aneurysm, RT3DE strongly correlated with reference values ( r =0 765-0 988), but 2DE weakly correlated with reference values ( r =0 518-0 592) There were no differences between RT3DE and reference values ( P >0 05), but a significant difference between 2DE and reference values occurred ( P <0 05) For excised canine hearts, there was a strong correlation between RT3DE and reference values ( r =0 728-0 914), while 2DE showed a less obvious correlation ( r =0 502-0 615) Again, there were no significant differenc 展开更多
关键词 real-time three-dimensional echocardiography · two-dimensional echocardiography · left ventricular volume · right ventricular volume
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新媒体时代的弹幕文化现象分析 被引量:38
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作者 付晶晶 《南京邮电大学学报(社会科学版)》 2016年第2期9-16,89,共9页
随着新媒体的不断发展,作为一种新兴文化现象的弹幕首先出现在小众范围的传播环境中。弹幕现象的出现,是社交网络发展的必然产物,也印证了网络世界群体狂欢行为的一种形式和可能。通过弹幕这种新型的视频观看形式,观看者对视频内容的共... 随着新媒体的不断发展,作为一种新兴文化现象的弹幕首先出现在小众范围的传播环境中。弹幕现象的出现,是社交网络发展的必然产物,也印证了网络世界群体狂欢行为的一种形式和可能。通过弹幕这种新型的视频观看形式,观看者对视频内容的共鸣体验得以实现。作为亚文化的弹幕由于具有典型的超时空的交互性、碎片化、反艺术、群体狂欢等特点,具备极大的发展潜力,进入主流文化的领域已逐渐成为现实。但弹幕在从小众到大众的发展道路上引发的争议不断,遇到的障碍和困难不可忽视。弹幕发言的自由并不等于恣意和无序,作为一种特殊的网络行为,同样需要他律和自律。 展开更多
关键词 新媒体 弹幕 弹幕文化 反艺术 二次元
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一种磁记忆检测定量分析的新方法 被引量:36
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作者 任吉林 王进 +2 位作者 范振中 宋凯 唐继红 《仪器仪表学报》 EI CAS CSCD 北大核心 2010年第2期431-436,共6页
金属磁记忆检测技术是利用磁记忆效应对铁磁性材料的应力集中区进行无损检测的新方法。磁记忆效应的特征是应力集中部位表面的漏磁场的切向分量最大、法向分量存在过零现象。在目前的研究工作中,研究方法主要是以测量磁记忆法向信号过... 金属磁记忆检测技术是利用磁记忆效应对铁磁性材料的应力集中区进行无损检测的新方法。磁记忆效应的特征是应力集中部位表面的漏磁场的切向分量最大、法向分量存在过零现象。在目前的研究工作中,研究方法主要是以测量磁记忆法向信号过零点位置来判断应力集中,从而割裂了切向与法向之间内在的关系。探讨了采用法向切向联合检测的方法,利用一种新型的磁阻传感器HMC1002,设计组装了高精度的二维弱磁场测量传感器。通过对拉伸试件进行2种方法检测的对比实验,结果表明,此方法比单一的法向磁场分量过零值点的检测更有效,可望在磁记忆检测定量分析研究中有很好的实际应用价值。 展开更多
关键词 磁记忆检测 二维 定量分析
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Recent advances in friction and lubrication of graphene and other 2D materials:Mechanisms and applications 被引量:37
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作者 Lincong LIU Ming ZHOU +6 位作者 Long JIN Liangchuan LI Youtang MO Guoshi SU Xiao LI Hongwei ZHU Yu TIAN 《Friction》 SCIE CSCD 2019年第3期199-216,共18页
Two-dimensional materials having a layered structure comprise a monolayer or multilayers of atomic thickness and ultra-low shear strength.Their high specific surface area,in-plane strength,weak layer-layer interaction... Two-dimensional materials having a layered structure comprise a monolayer or multilayers of atomic thickness and ultra-low shear strength.Their high specific surface area,in-plane strength,weak layer-layer interaction,and surface chemical stability result in remarkably low friction and wear-resisting properties.Thus,2D materials have attracted considerable attention.In recent years,great advances have been made in the scientific research and industrial applications of anti-friction,anti-wear,and lubrication of 2D materials.In this article,the basic nanoscale friction mechanisms of 2D materials including interfacial friction and surface friction mechanisms are summarized.This paper also includes a review of reports on lubrication mechanisms based on the film-formation,self-healing,and ball bearing mechanisms and applications based on lubricant additives,nanoscale lubricating films,and space lubrication materials of 2D materials in detail.Finally,the challenges and potential applications of 2D materials in the field of lubrication were also presented. 展开更多
关键词 two-dimensional MATERIALS GRAPHENE anti-friction ANTI-WEAR LUBRICATION
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Double contrast-enhanced two-dimensional and three-dimensional ultrasonography for evaluation of gastric lesions 被引量:37
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作者 Hong Shi Xiu-Hua Yu +7 位作者 Xin-Zhang Guo Yuan Guo Hong Zhang Bin Qian Zhang-Rui Wei Li Li Xian-Chen Wang Zi-Xiang Kong 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第31期4136-4144,共9页
AIM: To investigate the value of two-dimensional (2D) and three-dimensional (3D) double contrast-enhanced ultrasonography (DCUS) imaging for evaluation of gas- tric lesions. METHODS: 2D and 3D DCUS imaging wit... AIM: To investigate the value of two-dimensional (2D) and three-dimensional (3D) double contrast-enhanced ultrasonography (DCUS) imaging for evaluation of gas- tric lesions. METHODS: 2D and 3D DCUS imaging with both oral and intravenous administrations of contrast agents was used to assess gastroscopiclly-confirmed gastric lesions in 46 patients with benign and malignant diseases. Initially, liquid-based ultrasound contrast agent (Xinzhang) was given orally at dose of 500-600 mL for conventional ultrasound examination of the gastric lesions, and then a microbubble-based contrast agent (SonoVue) was injected intravenously at dose of 1.2-2.4 mL in bolus fashion to assess the perfusion pattern of the lesions using contrast imaging modes. The parameters derived from time-intensity curves including the arrival time (AT), time to peak (lq-P), peak intensity (PI) and en- hanced intensity (EI) were measured on the 2D DCUS imaging. 3D DCUS of the lesions was acquired to dem- onstrate the value of this imaging mode. RESULTS: There were 22 cases with benign lesions including chronic gastritis (n= 5), gastric ulcer (n = 9), gastric polyps (n = 3), gastric stromal tumors (n = 5), and 24 cases with malignant lesions including gastric cancer (n = 20), gastric cardia carcinoma (n = 3) and post-operative recurrent gastric cancer (n = 1) in the study, The oral contrast-enhanced ultrasonography (CEUS) imaging of the stomach clearly demonstrated the anatomy of the stomach and morphologic features of gastric lesions, With optimal scanning window and imaging display under oral CEUS, intravenous CEUS clearly showed the perfusion of gastric lesions with various characteristic manifestations. Both 2D and 3D DCUS images clearly demonstrated normal gastric wall as a three-layer structure, from the inside out, hypere- choic mucosa, hypoechoic muscularis and hyperechoic serosa, respectively. There were statistical significant differences of AT (8.68±2.06 vs 10.43 ± 展开更多
关键词 Contrast-enhanced ultrasonography Gastriclesions two-dimensional imaging Three-dimensionalimaging Contrast media
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彩色多普勒超声对甲状腺癌诊断的临床应用价值 被引量:32
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作者 郭艳 潘如凤 +2 位作者 宋伟 徐园园 吕宏安 《中华全科医学》 2013年第9期1454-1455,F0003,共3页
目的研究应用彩色多普勒超声诊断及鉴别诊断甲状腺癌的临床价值。方法回顾性分析我院有甲状腺结节者110例,其中良性结节119个,恶性结节83个,均经过手术及病理证实。结果 119个良性结节和83个恶性结节的二维及彩色多普勒超声的声像图特... 目的研究应用彩色多普勒超声诊断及鉴别诊断甲状腺癌的临床价值。方法回顾性分析我院有甲状腺结节者110例,其中良性结节119个,恶性结节83个,均经过手术及病理证实。结果 119个良性结节和83个恶性结节的二维及彩色多普勒超声的声像图特征进行分析对比,甲状腺癌的主要表现为结节外形不规则、内部回声杂乱,多为单发,多为低回声,血流走行杂乱,分枝型血流;脉冲多普勒显示的动脉血流为高阻力性血流。结果显示:结节的外形、包膜、内部回声、微粒钙化及血流分级、阻力指数等方面比较,两组差异均有统计意义(P<0.05)。良、恶性结节的动脉峰值血流流速比较差异统计学意义(P>0.05)。结论二维及彩色多普勒超声对甲状腺结节术前诊断及鉴别诊断具有较高的临床价值。 展开更多
关键词 二维超声 彩色多普勒 甲状腺癌
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Two-dimensional semiconductors with possible high room temperature mobility 被引量:32
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作者 Wenxu Zhang Zhishuo Huang Wanli Zhang Yanrong Li 《Nano Research》 SCIE EI CAS CSCD 2014年第12期1731-1737,共7页
We have calculated the longitudinal acoustic phonon limited electron mobility of 14 twvo-dimensional semiconductors with composition of MX2, where M (= Mo, W, Sn, Hf, Zr and Pt) is the transition metal, and X is S, ... We have calculated the longitudinal acoustic phonon limited electron mobility of 14 twvo-dimensional semiconductors with composition of MX2, where M (= Mo, W, Sn, Hf, Zr and Pt) is the transition metal, and X is S, Se and Te. We treated the scattering matrix by the deformation potential approximation. We found that out of 14 compounds, MoTe2, HfSe2 and ZrSe2 are promising regarding to their possible high mobility and finite band gap. The phonon limited mobility can be above 2,500 cm^2·V^-1·s^-1 at room temperature. 展开更多
关键词 electron mobility acoustic phonon ab initio two-dimensional (2D)materials
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Black phosphorus as a new lubricant 被引量:30
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作者 Wei WANG Guoxin XIE Jianbin LUO 《Friction》 SCIE CSCD 2018年第1期116-142,共27页
In recent years, a new 2D-layered material—black phosphorus(BP)—has been a rising star after the era of graphene owing to its high charge carrier mobility, tunable direct bandgap and unique in-plane anisotropic stru... In recent years, a new 2D-layered material—black phosphorus(BP)—has been a rising star after the era of graphene owing to its high charge carrier mobility, tunable direct bandgap and unique in-plane anisotropic structure. With the development of the synthesis and modification methods of BP, its extensive applications, e.g., transistors, batteries and optoelectronics have emerged. In order to explore its full potential, research into the tribological properties of BP 2D-layered materials such as lubrication additives and fillers in self-lubricating composite materials would be not only of high scientific value but also of practical significance. In this work, recent advances on the friction and lubrication properties of BP nanosheets made by our group, including the micro-friction properties, the lubrication properties of BP nanosheets as water-based and oil-based lubrication additives, and the friction and wear of BP/PVDF composites will be presented. Finally, the future challenges and opportunities in the use of BP materials as lubricants will be discussed. 展开更多
关键词 black phosphorus twodimensional (2D) material lubricant additive self-lubricating composite materials friction
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肝细胞癌患者血清蛋白质组成分的双向凝胶电泳-飞行时间质谱分析 被引量:17
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作者 王征 阮幼冰 官阳 《中华病理学杂志》 CAS CSCD 北大核心 2003年第4期333-336,共4页
目的 分析肝细胞癌患者与正常人血清双向凝胶电泳的差异蛋白质 ,寻找肝癌早期血清学诊断的可能指标。方法 固相 pH梯度双向凝胶电泳分离肝细胞癌患者及正常人血清总蛋白。银染显色 ,PDQuest 2DE软件分析 ,对差异蛋白质点用基质辅助激... 目的 分析肝细胞癌患者与正常人血清双向凝胶电泳的差异蛋白质 ,寻找肝癌早期血清学诊断的可能指标。方法 固相 pH梯度双向凝胶电泳分离肝细胞癌患者及正常人血清总蛋白。银染显色 ,PDQuest 2DE软件分析 ,对差异蛋白质点用基质辅助激光解吸电离飞行时间质谱 (MALDI TOF MS)测定其胶内酶解后的肽质指纹图谱 ,用PepIdent软件查询SWISS PROT数据库。 结果 获得了重复性较好的双向电泳银染图谱。图像分析检测 3块胶平均匹配的点数占平均蛋白点数的匹配率是 70 2 %。等电聚焦方向的平均偏差为 (1 0 2± 0 2 2 )mm ,十二烷基硫酸钠 聚丙烯酰胺凝胶电泳 (SDS PAGE)方向的平均偏差为 (0 97± 0 14 )mm。将 2 3个差异点进行胶内原位酶解 质谱指纹图分析 ,获得 15张肽质指纹图 ,查询数据库进行了初步鉴定。结论 固相 pH梯度双向凝胶电泳分离人血清总蛋白获得重复性较好的结果 ,但仍需进一步探索如何去除高丰度已知蛋白及脂类等物质的方法 ,以便得到分辨率更高的双向电泳银染图谱。MALDI TOF 展开更多
关键词 肝细胞癌 蛋白质 双向凝胶电泳 飞行时间质谱 MALDI-TOF-MS肽质指纹图
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二维振动铲装的试验研究 被引量:26
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作者 殷涌光 李俊明 王国强 《农业机械学报》 EI CAS CSCD 北大核心 1994年第2期18-23,共6页
通过小型铲斗改变振动参数的正交试验得出:振幅、频率、插入速度对铲土功和消耗功率均有较显著的影响;频率与插入速度对铲土功和消耗功率的影响一致,即高频率与低插入速度时铲土功与消耗功率较小;振幅对铲土功与功率消耗的影响相反... 通过小型铲斗改变振动参数的正交试验得出:振幅、频率、插入速度对铲土功和消耗功率均有较显著的影响;频率与插入速度对铲土功和消耗功率的影响一致,即高频率与低插入速度时铲土功与消耗功率较小;振幅对铲土功与功率消耗的影响相反,即小振幅时消耗功率小,大振幅时铲土功较小。 展开更多
关键词 振动切削 土壤 二维 功耗 装载机
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二维应变超声心动图的研究进展及临床应用 被引量:25
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作者 王洲 李健 +1 位作者 任永凤 朱天刚 《中国医学影像技术》 CSCD 北大核心 2008年第6期959-962,共4页
二维应变超声心动图是一种通过标准二维成像测量应变的新方法.没有角度依赖性,能够简单、快速、可重复性定量评价整体和局部心肌功能。
关键词 二维超声心动图描记术 斑点追踪 应变 应变率 室壁运动
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Chemical vapor deposition growth of monolayer MoSe2 nanosheets 被引量:29
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作者 Jonathan C. Shaw Hailong Zhou +4 位作者 Yu Chen Nathan O. Weiss Yuan Liu Yu Huang Xiangfeng Duan 《Nano Research》 SCIE EI CAS CSCD 2014年第4期511-517,共7页
The synthesis of two-dimensional (2D) layered materials with controllable thickness is of considerable interest for diverse applications. Here we report the first chemical vapor deposition growth of single- and few-... The synthesis of two-dimensional (2D) layered materials with controllable thickness is of considerable interest for diverse applications. Here we report the first chemical vapor deposition growth of single- and few-layer MoSe2 nanosheets. By using Se and MoO3 as the chemical vapor supply, we demonstrate that highly crystalline MoSe2 can be directly grown on the 300 nm SiO2/Si substrates to form optically distinguishable single- and multi-layer nanosheets, typically in triangular shaped domains with edge lengths around 30 btm, which can merge into continuous thin films upon further growth. Micro-Raman spectroscopy and imaging was used to probe the thickness-dependent vibrational properties. Photoluminescence spectroscopy demonstrates that MoSe2 monolayers exhibit strong near band edge emission at 1.55 eV, while bilayers or multi-layers exhibit much weaker emission, indicating of the transition to a direct band gap semiconductor as the thickness is reduced to a monolayer. 展开更多
关键词 chemical vapor deposition molybdenum diselenide two-dimensional materials transition metaldichalcogenide layered materials semiconductor
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Comparative proteomic analysis of differentially expressed proteins in human pancreatic cancer tissue 被引量:27
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作者 Chen, Jian-Hua Ni, Run-Zhou +2 位作者 Xiao, Ming-Bing Guo, Ji-Guang Zhou, Jia-Wei 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2009年第2期193-200,共8页
BACKGROUND: Pancreatic cancer is one of the most common malignant tumors. Early diagnosis of pancreatic cancer is difficult because of the latent onset and lack of good biomarkers. This study aimed to look for and ide... BACKGROUND: Pancreatic cancer is one of the most common malignant tumors. Early diagnosis of pancreatic cancer is difficult because of the latent onset and lack of good biomarkers. This study aimed to look for and identify differentially expressed proteins in tissues of pancreatic cancer and adjacent noncancerous tissues by proteomic approaches so as to provide information about possible pancreatic cancer markers and therapeutic targets. METHODS: Proteins extracted front 3 paired adjacent noncancerous and cancerous pancreatic tissue specimens were separated by two-dimensional gel electrophoresis (2-DE). The protein spots exhibiting statistical alternations between the two groups through computerized image analysis were then identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). In addition, Western blotting and immunohistochemistry were performed to verify the expression of certain candidate proteins. RESULTS: Twelve proteins were significantly upregulated and 4 were downregulated between cancerous and paired adjacent noncancerous pancreatic tissues. Several proteins (S100A11, Ig gamma-l chain C region, GSTO1 and peroxiredoxin 4) were found for the first time to be associated with pancreatic cancer. Differential expression of some identified proteins was further confirmed by Western blotting analysis and/or immunohistochemical analysis. CONCLUSIONS: Comparative proteomic analysis using 2-DE and MALDI-TOF-MS is an effective method for identifying differentially expressed proteins that may be the potential diagnostic biomarkers and therapeutic targets for pancreatic cancer. 展开更多
关键词 electrophoresis gel two-dimensional pancreatic neoplasms PROTEOMICS
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