Objective: To predict preoperative staging using a radiomics approach based on computed tomography (CT)images of patients with esophageal squamous cell carcinoma (ESCC).Methods: This retrospective study included...Objective: To predict preoperative staging using a radiomics approach based on computed tomography (CT)images of patients with esophageal squamous cell carcinoma (ESCC).Methods: This retrospective study included 154 patients (primary cohort: n: t 14; validation cohort: n:40) withpathologically confirmed ESCC. All patients underwent a preoperative CT scan from the neck to abdomen. Highthroughput and quantitative radiomics features were extracted from the CT images for each patient. A radiomicssignature was constructed using the least absolute shrinkage and selection operator (Lasso). Associations betweenradiomics signature, tumor volume and ESCC staging were explored. Diagnostic performance of radiomicsapproach and tumor volume for discriminating between stages Ⅰ-Ⅱand Ⅲ-Ⅳ was evaluated and compared usingthe receiver operating characteristics (ROC) curves and net reclassification improvement (NRI).Results= A total of 9,790 radiomics features were extracted. Ten features were selected to build a radiomicssignature after feature dimension reduction. The radiomics signature was significantly associated with ESCCstaging (P〈0.001), and yielded a better performance for discrimination of early and advanced stage ESCC comparedto tumor volume in both the primary [area under the receiver operating characteristic curve (AUC): 0.795 vs. 0.694,P=0.003; NRI=0.424)] and validation cohorts (AUC: 0.762 vs. 0.624, P=0.035; NRI=0.834).Conclusions: The quantitative approach has the potential to identify stage Ⅰ-Ⅱand Ⅲ-Ⅳ ESCC beforetreatment.展开更多
目的探讨常规MRI与弥散加权成像(DWI)测量的肿瘤容积对直肠癌TN分期的诊断价值。材料与方法对经肠镜确诊的74例直肠癌患者行盆腔3.0 T MRI检査。分析MRI对直肠癌术前TN分期的准确性,用检验评价MRI术前TN分期与病理分期的一致性。用单因...目的探讨常规MRI与弥散加权成像(DWI)测量的肿瘤容积对直肠癌TN分期的诊断价值。材料与方法对经肠镜确诊的74例直肠癌患者行盆腔3.0 T MRI检査。分析MRI对直肠癌术前TN分期的准确性,用检验评价MRI术前TN分期与病理分期的一致性。用单因素方差分析评价DWI(b=10000 s/mm^2)测量的肿瘤容积与病理TN分期的相关性。结果MRI诊断直肠癌T、N分期的准确率分别为87.8%(66/74)、66.2%(49/74);病理学和MRI对T分期诊断有较好的一致性(Kappa=0.78,P=0.000)、对N分期诊断一致性较差(Kappa=0.33,F=0.000)。在DWI上测量肿瘤容积≤T2期、T3期、T4期分别为(4145.13±718,00)mm^3、(14939.73±3591.38)mm^3、(22714.76±4251.71)mm^3;N0期、N1期、N2期分别为(14367.15±6425.83)mm^3,(17967.69±5259.88)mm^3、(19464.00±3588.77)DWI测肿瘤容积越大直肠癌T分期越高,差异有统计学意义(F=75.189,P=0.000);肿瘤容积与直肠癌不同N分期间差异有统计学意义(F=3.545,P=0.034)。结论MRI评价直肠癌T分期准确率较高,对N分期也有一定诊断价值。MR肿瘤容积测量对直肠癌TN分期有重要参考价值。展开更多
AIM:To analyze the value of computed tomography(CT) volume measurements for evaluation of the survival rate of unresectable hepatocellular carcinoma(HCC) patients after transcatheter arterial chemoembolization(TACE).M...AIM:To analyze the value of computed tomography(CT) volume measurements for evaluation of the survival rate of unresectable hepatocellular carcinoma(HCC) patients after transcatheter arterial chemoembolization(TACE).METHODS:One hundred and sixty-six unresectable HCC patients after TACE were involved in this retrospective study.Hepatic CT scan was performed for all patients before and 4 wk to 2 mo after TACE to def ine the morphologic features of HCC including its largest diameter,volume,product of the greatest axial dimension,tumor to liver volume ratio(TTLVR),and tumor shrinkage ratio.Clinical variables used in the study included gender,age,pattern of tumor growth,number of lesions,Child-Pugh classif ication of liver function,repeated TACE times,pre-or post-treatment α-fetoprotein(AFP) level,portal vein cancerous thrombus,tumor metastasis,degree of lipiodol retention within the tumor,and percutaneous ethanol injection.The correlation between survival time and clinical variables of patients or lesions was analyzed by combining morphologic features with the corresponding clinical and general data as input.A Cox proportional hazard model was used to analyze prognostic factors.The Kaplan-Meier method was used to calculate the cumulative survival time.In? uence of the parameters on prognosis was analyzed by the log-rank test.RESULTS:The overall 6,12,24,36 and 60 mo cumulative survival rates were 78.92%,49.85%,23.82%,15.60% and 8.92%,respectively.The median survival time was 12 mo.Univariate and multivariate analysis showed that only 4 parameters were the independent prognostic factors including TTLVR(χ2 = 14.328,P < 0.001),portal vein cancerous thrombus(χ2 = 5.643,P = 0.018),repeated TACE times(χ2 = 8.746,P = 0.003),and post-treatment serum AFP level(χ2 = 5.416,P = 0.020).When the TTLVR value was less than 70%,the survival time was inversely correlated with the TTLVR value.CONCLUSION:CT volume measurement technique can predict the prognosis of unresectable HCC patients after TACE.展开更多
The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (...The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (CNE-2Z cells). Both migrated and non-migrated CNE-2Z cells, when swollen by 47% hypotonic solution, exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5’-triphosphate (ATP), 5-nitro-2-(3- phenylpropylamino) benzoic acid (NPPB) and tamoxifen. However, RVD rate in migrated CNE-2Z cells was bigger than that of non-migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non- migrated cells. ATP, NPPB and tamoxifen also inhibited migration of CNE-2Z cells. The inhibition of migration was positively correlated to the blockage of RVD, with a correlation coefficient (r) = 0.99, suggesting a functional relation- ship between RVD and cell migration. We conclude that RVD is involved in cell migration and RVD may play an important role in migratory process in CNE-2Z cells.展开更多
基金supported by the National Key R&D Program of China (No. 2017YFC1309100)National Natural Scientific Foundation of China (No. 81771912)Science and Technology Planning Project of Guangdong Province (No. 2017B020227012)
文摘Objective: To predict preoperative staging using a radiomics approach based on computed tomography (CT)images of patients with esophageal squamous cell carcinoma (ESCC).Methods: This retrospective study included 154 patients (primary cohort: n: t 14; validation cohort: n:40) withpathologically confirmed ESCC. All patients underwent a preoperative CT scan from the neck to abdomen. Highthroughput and quantitative radiomics features were extracted from the CT images for each patient. A radiomicssignature was constructed using the least absolute shrinkage and selection operator (Lasso). Associations betweenradiomics signature, tumor volume and ESCC staging were explored. Diagnostic performance of radiomicsapproach and tumor volume for discriminating between stages Ⅰ-Ⅱand Ⅲ-Ⅳ was evaluated and compared usingthe receiver operating characteristics (ROC) curves and net reclassification improvement (NRI).Results= A total of 9,790 radiomics features were extracted. Ten features were selected to build a radiomicssignature after feature dimension reduction. The radiomics signature was significantly associated with ESCCstaging (P〈0.001), and yielded a better performance for discrimination of early and advanced stage ESCC comparedto tumor volume in both the primary [area under the receiver operating characteristic curve (AUC): 0.795 vs. 0.694,P=0.003; NRI=0.424)] and validation cohorts (AUC: 0.762 vs. 0.624, P=0.035; NRI=0.834).Conclusions: The quantitative approach has the potential to identify stage Ⅰ-Ⅱand Ⅲ-Ⅳ ESCC beforetreatment.
文摘目的探讨常规MRI与弥散加权成像(DWI)测量的肿瘤容积对直肠癌TN分期的诊断价值。材料与方法对经肠镜确诊的74例直肠癌患者行盆腔3.0 T MRI检査。分析MRI对直肠癌术前TN分期的准确性,用检验评价MRI术前TN分期与病理分期的一致性。用单因素方差分析评价DWI(b=10000 s/mm^2)测量的肿瘤容积与病理TN分期的相关性。结果MRI诊断直肠癌T、N分期的准确率分别为87.8%(66/74)、66.2%(49/74);病理学和MRI对T分期诊断有较好的一致性(Kappa=0.78,P=0.000)、对N分期诊断一致性较差(Kappa=0.33,F=0.000)。在DWI上测量肿瘤容积≤T2期、T3期、T4期分别为(4145.13±718,00)mm^3、(14939.73±3591.38)mm^3、(22714.76±4251.71)mm^3;N0期、N1期、N2期分别为(14367.15±6425.83)mm^3,(17967.69±5259.88)mm^3、(19464.00±3588.77)DWI测肿瘤容积越大直肠癌T分期越高,差异有统计学意义(F=75.189,P=0.000);肿瘤容积与直肠癌不同N分期间差异有统计学意义(F=3.545,P=0.034)。结论MRI评价直肠癌T分期准确率较高,对N分期也有一定诊断价值。MR肿瘤容积测量对直肠癌TN分期有重要参考价值。
文摘AIM:To analyze the value of computed tomography(CT) volume measurements for evaluation of the survival rate of unresectable hepatocellular carcinoma(HCC) patients after transcatheter arterial chemoembolization(TACE).METHODS:One hundred and sixty-six unresectable HCC patients after TACE were involved in this retrospective study.Hepatic CT scan was performed for all patients before and 4 wk to 2 mo after TACE to def ine the morphologic features of HCC including its largest diameter,volume,product of the greatest axial dimension,tumor to liver volume ratio(TTLVR),and tumor shrinkage ratio.Clinical variables used in the study included gender,age,pattern of tumor growth,number of lesions,Child-Pugh classif ication of liver function,repeated TACE times,pre-or post-treatment α-fetoprotein(AFP) level,portal vein cancerous thrombus,tumor metastasis,degree of lipiodol retention within the tumor,and percutaneous ethanol injection.The correlation between survival time and clinical variables of patients or lesions was analyzed by combining morphologic features with the corresponding clinical and general data as input.A Cox proportional hazard model was used to analyze prognostic factors.The Kaplan-Meier method was used to calculate the cumulative survival time.In? uence of the parameters on prognosis was analyzed by the log-rank test.RESULTS:The overall 6,12,24,36 and 60 mo cumulative survival rates were 78.92%,49.85%,23.82%,15.60% and 8.92%,respectively.The median survival time was 12 mo.Univariate and multivariate analysis showed that only 4 parameters were the independent prognostic factors including TTLVR(χ2 = 14.328,P < 0.001),portal vein cancerous thrombus(χ2 = 5.643,P = 0.018),repeated TACE times(χ2 = 8.746,P = 0.003),and post-treatment serum AFP level(χ2 = 5.416,P = 0.020).When the TTLVR value was less than 70%,the survival time was inversely correlated with the TTLVR value.CONCLUSION:CT volume measurement technique can predict the prognosis of unresectable HCC patients after TACE.
基金This work was supported by grants from the Wellcome Trust UK(056909/299/Z)Ministry of Education of China(GJ9901)+1 种基金the Health Department of Guangdong Province(A2001474)Guangdong Medical College Fund for Young Scholars(XQ0306).
文摘The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (CNE-2Z cells). Both migrated and non-migrated CNE-2Z cells, when swollen by 47% hypotonic solution, exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5’-triphosphate (ATP), 5-nitro-2-(3- phenylpropylamino) benzoic acid (NPPB) and tamoxifen. However, RVD rate in migrated CNE-2Z cells was bigger than that of non-migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non- migrated cells. ATP, NPPB and tamoxifen also inhibited migration of CNE-2Z cells. The inhibition of migration was positively correlated to the blockage of RVD, with a correlation coefficient (r) = 0.99, suggesting a functional relation- ship between RVD and cell migration. We conclude that RVD is involved in cell migration and RVD may play an important role in migratory process in CNE-2Z cells.