Micromelalopha troglodyta （Graeser） is an important pest of poplar in China. Glutathione S-transferases （GSTs） are known to be responsible for adaptation mechanisms of M. troglodyta. The activities and kinetic con...Micromelalopha troglodyta （Graeser） is an important pest of poplar in China. Glutathione S-transferases （GSTs） are known to be responsible for adaptation mechanisms of M. troglodyta. The activities and kinetic constants of glutathione S-transferases in M. troglodyta were studied. Significant differences in glutathione S-transferase activity and kinetic characteristics were observed among five instars of M. troglodyta larvae. Furthermore, the inhibition of glutathione S-transferase activity in five instars by 24 inhibitors was conducted. The results show the inhibition of GST activity of different instars by 24 inhibitors was different. For GST activity in the 1st instar, chlorpyrifos, lambda-cyhalothrin, endosulfan, abamectin, fipronil and pyridaben were the best inhibitors tested, and for GST activity in the 2nd instar, tannic acid and quercetin were the most potent inhibitors tested, and for GST activity in the 3rd instar, the inhibitory effects of quercetin, chlorpyrifos and lambda-cyhalothrin were the highest, and for GST activity in the 4th instar, quercetin and lambda-cyhalothrin were the best inhibitors, and the inhibitory effect of phoxim was the highest for GST activity in the 5th instar. Our results show that glutathione S-transferases in different instars are qualitatively different in isozyme composition and thus different in sensitivity to inhibitors.展开更多
This paper deals with the bionomics of Micromelopha troglodyta .The pest has 2 generations a year and overwinters in pupa in the fallen leaves.In the food lack condition,the last instar larvae is compelled to pupate i...This paper deals with the bionomics of Micromelopha troglodyta .The pest has 2 generations a year and overwinters in pupa in the fallen leaves.In the food lack condition,the last instar larvae is compelled to pupate in advance,the mass of the pupa is much lighter than that of normal pupa.If the mass of the pupa is below 90 mg,it can not emerge.The mathematical model between the probability of the female and the pupa mass is Y i =-6.22+3.256 log X i .The mathematical model between amount of the female eggs and the pupa mass is Y i′ =-1 886.3+1 014.4 log X i .Applying these mathematical models,we can forecast the population dynamics of M.troglodyta.展开更多
基金supported by National Natural Science Foundation of China(Contract No.30600476 and 30972376)
文摘Micromelalopha troglodyta （Graeser） is an important pest of poplar in China. Glutathione S-transferases （GSTs） are known to be responsible for adaptation mechanisms of M. troglodyta. The activities and kinetic constants of glutathione S-transferases in M. troglodyta were studied. Significant differences in glutathione S-transferase activity and kinetic characteristics were observed among five instars of M. troglodyta larvae. Furthermore, the inhibition of glutathione S-transferase activity in five instars by 24 inhibitors was conducted. The results show the inhibition of GST activity of different instars by 24 inhibitors was different. For GST activity in the 1st instar, chlorpyrifos, lambda-cyhalothrin, endosulfan, abamectin, fipronil and pyridaben were the best inhibitors tested, and for GST activity in the 2nd instar, tannic acid and quercetin were the most potent inhibitors tested, and for GST activity in the 3rd instar, the inhibitory effects of quercetin, chlorpyrifos and lambda-cyhalothrin were the highest, and for GST activity in the 4th instar, quercetin and lambda-cyhalothrin were the best inhibitors, and the inhibitory effect of phoxim was the highest for GST activity in the 5th instar. Our results show that glutathione S-transferases in different instars are qualitatively different in isozyme composition and thus different in sensitivity to inhibitors.
文摘This paper deals with the bionomics of Micromelopha troglodyta .The pest has 2 generations a year and overwinters in pupa in the fallen leaves.In the food lack condition,the last instar larvae is compelled to pupate in advance,the mass of the pupa is much lighter than that of normal pupa.If the mass of the pupa is below 90 mg,it can not emerge.The mathematical model between the probability of the female and the pupa mass is Y i =-6.22+3.256 log X i .The mathematical model between amount of the female eggs and the pupa mass is Y i′ =-1 886.3+1 014.4 log X i .Applying these mathematical models,we can forecast the population dynamics of M.troglodyta.
