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三叶草基因工程研究进展 被引量:24
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作者 梁哲 姜三杰 +2 位作者 未丽 唐益雄 吴燕民 《草业学报》 CSCD 北大核心 2009年第2期205-211,共7页
三叶草作为优良的牧草,具有营养丰富、品质优良、适口性好等特点。现代生物技术的不断发展为三叶草育种和种质创新提供了新的技术手段,笔者从三叶草再生和遗传转化体系,转基因三叶草在品质改良、抗病性、抗虫性、抗逆性及作为植物反应... 三叶草作为优良的牧草,具有营养丰富、品质优良、适口性好等特点。现代生物技术的不断发展为三叶草育种和种质创新提供了新的技术手段,笔者从三叶草再生和遗传转化体系,转基因三叶草在品质改良、抗病性、抗虫性、抗逆性及作为植物反应器等方面的研究进展进行了全面综述,分析了三叶草基因工程研究中存在的主要问题,并对其应用前景和研究方向进行了展望。 展开更多
关键词 三叶草 再生体系 转化体系 转基因
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18个三叶草品种耐热性综合评价 被引量:22
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作者 张鹤山 刘洋 +2 位作者 王凤 田宏 蔡化 《草业科学》 CAS CSCD 北大核心 2009年第7期44-49,共6页
对18个三叶草品种耐热性进行了比较,并结合芽期和成苗试验,综合评价了各品种的耐热性强弱。结果表明:发芽率、发芽指数随温度的升高而降低,在40℃时种子不发芽;叶片灼伤率、植株存活率和叶片相对含水量在各品种之间差异较大。对耐热性... 对18个三叶草品种耐热性进行了比较,并结合芽期和成苗试验,综合评价了各品种的耐热性强弱。结果表明:发芽率、发芽指数随温度的升高而降低,在40℃时种子不发芽;叶片灼伤率、植株存活率和叶片相对含水量在各品种之间差异较大。对耐热性综合评价表明,18个品种中特特里红三叶属强耐热品种;新西兰白三叶耐热性较强;海法白三叶、胡依阿白三叶、G18白三叶、戈伦红三叶、雷托白三叶、巴东红三叶、帕韦拉红三叶、鄂牧1号白三叶、罗汤地红三叶、新西兰杂三叶、2-2-1杂三叶为中等耐热品种;克劳白三叶、皮陶白三叶、奥罗拉杂三叶和蒂特拉杂三叶品种耐热性较弱,耐热性最弱的是路易斯安娜白三叶。建议在长江流域及长江以南地区选择耐热性较强的几个品种进行推广示范。 展开更多
关键词 三叶草 耐热性 综合评价
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NaCl胁迫对三叶草种子萌发的影响 被引量:16
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作者 张侠 宋莉璐 +5 位作者 魏艳丽 任艳 朱娇 李红梅 李纪顺 杨合同 《山东科学》 CAS 2008年第5期11-14,共4页
研究了不同浓度NaCl溶液胁迫对从澳大利亚引进的3种三叶草(大花三叶草Trifolium michelianum,草莓三叶草Trifolium fragiferum和波斯三叶草Trifolium resupinatum)种子萌发及幼苗生长等相关生理生化特性的影响。研究结果表明NaCl胁迫对... 研究了不同浓度NaCl溶液胁迫对从澳大利亚引进的3种三叶草(大花三叶草Trifolium michelianum,草莓三叶草Trifolium fragiferum和波斯三叶草Trifolium resupinatum)种子萌发及幼苗生长等相关生理生化特性的影响。研究结果表明NaCl胁迫对三叶草种子萌发和幼苗生长有一定的抑制作用,且抑制程度随着浓度的提高而增加。综合萌发率、芽长、根长、发芽指数、活力指数和种子膜透性等指标,草莓三叶草(Trifolium fragiferum?)表现出较其他2种三叶草更好的耐盐性,具有可推广潜力。 展开更多
关键词 三叶草 盐胁迫 萌发
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Study on the Degradation Trend of Ryegrass-White Clover Artificial Grassland 被引量:12
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作者 张建波 李相林 +4 位作者 万里强 陈瑞祥 赵相勇 陈伟 陈国南 《Agricultural Science & Technology》 CAS 2009年第1期26-28,88,共4页
[Objective] The study aimed to explore the degradation law and trend of artificial grassland. [Method] Taking the ryegrass (Lolium perenne) - white clover ( Trifolium repens) artificial grassland in Maiping Townsh... [Objective] The study aimed to explore the degradation law and trend of artificial grassland. [Method] Taking the ryegrass (Lolium perenne) - white clover ( Trifolium repens) artificial grassland in Maiping Township, Guizhou Province as the research object, the grassland vegetation of 40 quadrate from different areas (area around the sheep shed, hilltop, hillside, flatland at the foot of the hill) were analyzed by comparing the dominance and richness index. [ Result] Degradation of different degrees appeared in various areas of this artificial grassland. To be specific, the degradation of grassland around the sheep shed was not serious for less consumption by the sheep; however, the areas on the hilltop and hillside degenerated to the grassland types of dallis grass ( Paspalum dilatatum Poir. ) -Indian lovegrass ( Eragrostis pilosa) and dallis grass - cogongrass [ Imperata cylindrica ( Linn. ) Beauv. ], respectively, and the area at the foot of the hill degenerated to the grassland type dominated by garland chrysanthetnum ( Chrysanthemum coronarium L. ) and knotgrass ( Paspalum distichum L. ). [ Conclusion ] This study provided a basis for grassland improvement as well as the efficient and sustainable utilization of grazing-type artificial grassland in South China. 展开更多
关键词 Ryegrass Lolium perenne) White clover( trifolium repens) Artificial grassland DEGRADATION
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三叶青提取物抗ANIT所致小鼠肝损伤作用的研究 被引量:10
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作者 李萍 吉薇薇 彭昕 《中国现代医生》 2018年第30期32-35,共4页
目的研究三叶青提取物对α-异硫氰酸萘酯(ANIT)所致肝损伤小鼠的保护作用。方法 2017年1月~2018年2月选择60只小鼠并随机分为6组,分别为对照组、联苯双酯阳性药物组、模型组、三叶青提取物低、中、高剂量组[(1.0、2.0、4.0)g/kg]。采用... 目的研究三叶青提取物对α-异硫氰酸萘酯(ANIT)所致肝损伤小鼠的保护作用。方法 2017年1月~2018年2月选择60只小鼠并随机分为6组,分别为对照组、联苯双酯阳性药物组、模型组、三叶青提取物低、中、高剂量组[(1.0、2.0、4.0)g/kg]。采用灌胃ANIT的方式复制急性黄疸型肝炎模型,造模48 h后测其血清中ALT、AST、TBA、TBIL、TNF-α的含量;检测肝组织匀浆中SOD、MDA、GSH-Px的水平。结果三叶青提取物各剂量组均可显著降低TBIL和TNF-α水平(P<0.05),中、高剂量组可以降低血清中ALT、AST、TBA、TBIL、TNF-α含量(P<0.05),各剂量组均显著降低MDA含量,中、高剂量组可显著提高SOD活性(P<0.05),而GSH-Px水平无显著变化。结论三叶青提取物对ANIT所致小鼠肝损伤有一定的保护作用,其保护作用可能与减轻炎症因子产生、促进总胆红素的代谢、降低脂质过氧化程度有关。 展开更多
关键词 三叶青 ANIT 肝损伤 抗氧化
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五种重金属胁迫对白三叶和红三叶种子萌发及幼苗生长的影响 被引量:10
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作者 宋凤鸣 刘建华 +2 位作者 吴彩琼 雷江丽 徐义炎 《亚热带植物科学》 2014年第4期276-280,共5页
通过发芽试验探讨镉、锰、铅、铜、锌五种重金属离子胁迫对海发白三叶和红三叶种子萌发的影响。结果表明:海发白三叶和红三叶较耐镉和锰,铅、铜和锌次之。在国家土壤三级标准的镉离子浓度和800 mg·L-1锰离子浓度胁迫下仍有80%以上... 通过发芽试验探讨镉、锰、铅、铜、锌五种重金属离子胁迫对海发白三叶和红三叶种子萌发的影响。结果表明:海发白三叶和红三叶较耐镉和锰,铅、铜和锌次之。在国家土壤三级标准的镉离子浓度和800 mg·L-1锰离子浓度胁迫下仍有80%以上的发芽率,且幼苗均能保持较好活性。高浓度重金属离子对两种三叶草胚轴和胚根生长有显著抑制,红三叶发芽率整体略优于海发白三叶;两种三叶草均适合于镉、锰污染的土壤修复。 展开更多
关键词 三叶草 重金属离子 胁迫 种子萌发
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三叶草的播种试验 被引量:10
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作者 刘卫东 张端良 郭彤 《经济林研究》 2004年第3期20-22,共3页
对三叶草的2个种的播种、出苗、成坪一系列观察研究结果表明,白三叶海弗(畜)生长较好;白三叶瑞文德(北)、白三叶瑞文德(丹)、红三叶罗亚(兰)和红三叶罗亚(畜)生长适宜;白三叶瑞文德(畜)和白三叶休依(畜)表现欠佳。
关键词 白三叶 三叶草 播种试验 生长 出苗 红三叶 观察研究 适宜 表现
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干旱胁迫下5种三叶草的生理反应(英文) 被引量:8
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作者 蔺万煌 HOFMANN Rainer W STILWELL Stephen A 《应用与环境生物学报》 CAS CSCD 北大核心 2011年第4期580-584,共5页
为了解三叶草属抗旱的生理机理,寻找适宜干旱地区种植的三叶草品种,比较了地三叶草(Trifoliumsubterraneum L.)、大花三叶草(rmichelianum Savi)、自:二三叶草(rrepens L.)、高加索三叶草(T.ambiguum M.Bieb.)和草莓三... 为了解三叶草属抗旱的生理机理,寻找适宜干旱地区种植的三叶草品种,比较了地三叶草(Trifoliumsubterraneum L.)、大花三叶草(rmichelianum Savi)、自:二三叶草(rrepens L.)、高加索三叶草(T.ambiguum M.Bieb.)和草莓三叶草(zfragiferum L.)在人工干旱条件下4wk的生长和生理反应.结果表明,干旱处理下大仡乏叶草、草莓三叶草和白三叶草地上部分干重严重减少,分别降低了70%、66%和64%,而高加索三叶草和地三叶草减少不到50%;高加索三叶草叶片水势降低到-2.0MPa以下,而其他4种三叶草只降低到-1.5MPa左右;高加索三叶草和争莓三叶草叶片光合速率降低25%,而地三叶草、白三叶草和大花三叶草下降35%~63%.此外,干旱条件下,5种供试三叶草叶片中游离脯氨酸含量普遍增加,其中高加索三叶草和白三叶草分别增加到2.5倍和3.1倍.研究显示高加索三叶草在多项重要生长和生理指标中表现出很强的抗旱性,因此认为其可作为干旱地区栽培自叶草的补充或替代种. 展开更多
关键词 三叶草 干旱胁迫 游离脯氨酸 光合作用 水势
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三叶草的研究新进展 被引量:7
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作者 王亚茜 陈楠楠 +2 位作者 林晶晶 黄俊逸 廖鲜艳 《食品研究与开发》 CAS 北大核心 2016年第5期197-201,共5页
三叶草被认为是目前最有开发前途的保健食品和医药新资源之一,也是目前食品保健、生物医学等领域的研究热点。主要介绍了目前国内外对三叶草主要活性成分、功能及其应用等方面的研究进展,并对其进一步的开发进行了展望。
关键词 三叶草 活性成分 抗氧化 抑菌
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丛枝菌根真菌对车轴草属植物生长影响的Meta分析 被引量:7
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作者 秦明森 关佳威 +4 位作者 刘永俊 潘建斌 石国玺 蒋胜竞 冯虎元 《草业科学》 CAS CSCD 北大核心 2015年第10期1576-1585,共10页
应用Meta分析(Meta analysis)方法定量研究了接种丛枝菌根(Arbuscular Mycorrhizae,AM)真菌对车轴草属(Trifolium)植物生物量和营养吸收的影响。通过Web of Science数据库检索,共收集得到30篇原始文献,提取得到525个独立样本值。结果... 应用Meta分析(Meta analysis)方法定量研究了接种丛枝菌根(Arbuscular Mycorrhizae,AM)真菌对车轴草属(Trifolium)植物生物量和营养吸收的影响。通过Web of Science数据库检索,共收集得到30篇原始文献,提取得到525个独立样本值。结果表明,接种AM真菌能够显著增加车轴草属植物的总生物量、地上生物量和地下生物量,促进其对氮、磷的吸收。不同的AM真菌种类以及车轴草种类均对接种效果有影响,其中珠状巨孢囊霉(Gigaspora margarita)对车轴草属植物的生物量的促进效应最大;埃及车轴草(T.alexandrinum)地上、地下生物量对接种AM真菌的响应最强。胁迫处理能显著降低AM真菌对地上、地下生物量的接种效应。随培养时间延长,AM真菌能够显著增加氮、钾、锌吸收的接种效应。 展开更多
关键词 三叶草属 丛枝菌根真菌 META分析 接种 效应值
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Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn,against pathogenic bacteria causing tropical diseases 被引量:4
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作者 Abdul Viqar Khan Qamar Uddin Ahmed +1 位作者 Indu Shukla Athar Ali Khan 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2012年第3期189-194,共6页
Objective:To investigate antibacterial potential of Trifolium alexandrinum(T.alexandrinum) Linn,against seven gram positive and eleven gram negative hospital isolated human pathogenic bacterial strains responsible for... Objective:To investigate antibacterial potential of Trifolium alexandrinum(T.alexandrinum) Linn,against seven gram positive and eleven gram negative hospital isolated human pathogenic bacterial strains responsible for many tropical diseases.Methods:Non-polar and polar extracts of the leaves of T.alexandrinum i.e.,hexane,dichloromethane(DCM),ethyl acetate(EtOAc), methanol(MeOH) and aqueous(AQ) extracts at five different concentrations(1,2,5,10 and 15 mg/ mL) were prepared to evaluate their antibacterial value.NCCL standards were strictly followed to perform antimicrobial disc susceptibility test using disc diffusion method.Results:Polar extracts demonstrated significant antibacterial activity against tested pathogens.EtOAc and MeOH extracts showed maximum antibacterial activity with higher inhibition zone and were found effective against seventeen of the tested pathogens.While AQ plant extract inhibited the growth of sixteen of the test strains.EtOAc and MeOH plant extracts inhibited the growth of all seven gram positive and ten of the gram negative bacterial strains.Conclusions:The present study strongly confirms the effectiveness of crude leaves extracts against tested human pathogenic bacterial strains causing several tropical diseases.Since Egyptian clover is used as a fodder plant,it could be helpful in controlling various infectious diseases associated with cattle as well. 展开更多
关键词 trifolium alexandrinum L. FABACEAE Antibacterial activity Pathogenic BACTERIA GRAM-POSITIVE BACTERIA GRAM-NEGATIVE BACTERIA Tropical DISEASE Infectious DISEASE
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Semisynthetic Derivatives of Sissotrin Isolated from Trifolium baccarinii Chiov. (Fabaceae) and Evaluation of Their Antibacterial Activities
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作者 Donald Léonel Feugap Tsamo Cyrille Ngoufack Tagousop +2 位作者 Arnaud Joseph Nguetse Dongmo Laurence Voutquenne-Nazabadioko David Ngnokam 《Advances in Biological Chemistry》 CAS 2024年第1期27-39,共13页
Starting from sissotrin (1), a natural isoflavonoid isolated from Trifolium baccarinii (Fabaceae), one new semisynthetic derivative, 6-nitrobiochanin A (1b) and two known derivatives, 8-nitrobiochanin A (1a) and 2&quo... Starting from sissotrin (1), a natural isoflavonoid isolated from Trifolium baccarinii (Fabaceae), one new semisynthetic derivative, 6-nitrobiochanin A (1b) and two known derivatives, 8-nitrobiochanin A (1a) and 2",3",4",6"-tetraacetylsissotrin (1c) have been obtained after performing nitration and acetylation reactions. Their structures were assigned after interpretation of their spectrometric (HR-ESI-MS) and spectroscopic (NMR 1D and 2D) data and by comparison with those reported in the literature. The substrate as well as the semisynthetic derivatives were evaluated for their antibacterial activities against six strains. The results reveal that they are inactive or weakly active on the strains tested with the exception of 8-nitrobiochanin A (1a) which showed moderate activity (MIC = 62.5 μg∙mL<sup>−1</sup>) on Staphylococcus aureus ATCC 43300. 展开更多
关键词 trifolium baccarinii ISOFLAVONOID NITRATION ACETYLATION Antibacterial Activity
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白三叶根系分泌物对5种草坪草的生化效应 被引量:4
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作者 余婷 温艳斌 +3 位作者 孟焕文 刘同金 赵慧玲 程智慧 《草地学报》 CAS CSCD 北大核心 2014年第1期158-165,共8页
为探讨根系分泌物在白三叶(Trifolium repens L.)入侵禾本科草坪过程中的生化效应,采用水培法收集其根系分泌物,检测不同浓度白三叶根系分泌物对多年生黑麦草(Lolium perenne L.)、紫羊茅(FestucarubraL.)、高羊茅(Festuca arundinacea ... 为探讨根系分泌物在白三叶(Trifolium repens L.)入侵禾本科草坪过程中的生化效应,采用水培法收集其根系分泌物,检测不同浓度白三叶根系分泌物对多年生黑麦草(Lolium perenne L.)、紫羊茅(FestucarubraL.)、高羊茅(Festuca arundinacea Schreb.)、草地早熟禾(Poapratensis L.)和匍匐翦股颖(Agrostis stolonifera L.)叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性和丙二醛(MDA)含量以及根系活力的影响。结果表明:白三叶根系分泌物对5种草坪草上述生理生化指标均具有浓度效应。在低浓度下(0.002 g·mL^(-1))对SOD和CAT活性具有促进效应,在高浓度下(0.05 g·mL^(-1))则具有抑制效应。5种受体的POD活性和MDA含量均随白三叶根系分泌物浓度增大而升高,根系活力则随着其浓度增大而降低。因此,白三叶根系分泌物可引起受体植物对逆境的响应,5种草坪草化感效应的生化机制可能是分泌物中化感物质通过改变受体幼苗体内保护酶活性及脂膜过氧化来表现的。 展开更多
关键词 白三叶 根系分泌物 草坪草 化感作用 生化指标 trifolium repens L
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Ca^(2+)提高白花苜蓿抗冷性的研究 被引量:3
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作者 刘美艳 张健 张伟 《广西植物》 CAS CSCD 北大核心 2004年第2期174-177,共4页
Ca2 + 处理能缓解冷胁迫时白花苜蓿叶片叶绿素的降解速度 ,提高叶片中可溶性糖、脯氨酸和Vc的含量 ;还能保持冷胁迫时细胞膜透性的稳定 ,减少O 2 和MDA的积累 ;维持较高的SOD和POD的活性。
关键词 CA^2+ 白花苜蓿 抗冷性 冷胁迫 细胞膜透性
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三叶草根—土复合体抗剪切强度试验研究 被引量:6
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作者 方便 罗海波 魏华炜 《贵州大学学报(自然科学版)》 2012年第2期29-32,共4页
本文以三叶草为样本,研究其含根量分别为0.0 g/60 cm3、0.1 g/60 cm3、0.3 g/60 cm3、0.5 g/60 cm3、0.7 g/60 cm3和1.0 g/60 cm3的根—土复合体抗剪切强度,试验中对三叶草根-土复合体进行了法向应力分别为100 kPa、200 kPa、300 kPa及4... 本文以三叶草为样本,研究其含根量分别为0.0 g/60 cm3、0.1 g/60 cm3、0.3 g/60 cm3、0.5 g/60 cm3、0.7 g/60 cm3和1.0 g/60 cm3的根—土复合体抗剪切强度,试验中对三叶草根-土复合体进行了法向应力分别为100 kPa、200 kPa、300 kPa及400 kPa下的抗剪强度。结果表明:三叶草根系增加了土体的抗剪强度;根—土复合体在同一含根量下,其抗剪强度均随着法向应力的增加而增加;在同一法向应力下,随着含根量的增大,根—土复合体的抗剪强度、粘聚力和内摩擦角均呈现出先增大后减小的趋势,均在含根量为0.5 g/60 cm3时出现最大值。三叶草含根量为0.5-0.7 g/60 cm3时护坡效果最好。 展开更多
关键词 生态护坡 抗剪强度 根—土复合体 含根量 三叶草
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Effect of Trifolium pratense extract on methionine-choline- deficient diet-induced steatohepatitis in C57BL/6 mice 被引量:4
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作者 CHEN Tong ZHONG Fo-Jin +3 位作者 HONG Ya-Min SU Wei-Jiao ZHUANG Li-Li QIU Long-Xin 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2014年第3期194-198,共5页
AIM: The potential of Trifolium pratense (red clover) extract in the prevention of lipid disorder has attracted increasing attention in recent years. In this study, the aim was to determine whether and how red clov... AIM: The potential of Trifolium pratense (red clover) extract in the prevention of lipid disorder has attracted increasing attention in recent years. In this study, the aim was to determine whether and how red clover extract affected the development of murine diet-induced non-alcoholic steatohepatitis. METHOD: Non-alcoholic steatohepatitis was induced in C57BL/6 mice by feeding mice with a methionine-choline-deficient (MCD) diet. Hematoxylin and eosin staining was used for histological analyses. Real-time PCR was used to analyze the mRNA expression levels. RESULTS: Hepatic steatosis and neeroinflammation was observed in MCD diet-fed mice, and this diet-induced steatosis was sig- nificantly attenuated, whereas liver inflammation was not significantly attenuated, by red clover extract treatment. Consistent with the results of H&E staining, the MCD diet-induced increase of liver triglycerides and cholesterol levels were significantly reduced by red clover extract treatment. However, with the improvement in hepatic steatosis, mRNA levels of acetyl CoA oxidase, carnitine palrnitoyl transferase-l, and liver fatty acid-binding protein, three genes regulated by peroxisome proliferator-activated receptor (PPAR) ct, were unaffected. CONCLUSION: Red clover extract alleviated MCD diet-induced hepatic steatosis, but did not ameliorate liver inflammation in C57BL/6 mice, and the improvement in hepatic steatosis was not through activating PPAR^t. 展开更多
关键词 trifolium pratense STEATOHEPATITIS Hepatic steatosis Liver inflammation Peroxisome prolifemtor-activated receptor a
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火焰原子吸收光谱法测定红车轴草及其提取物中铁和锰 被引量:4
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作者 李松 黎国兰 +1 位作者 王洪褔 边清泉 《理化检验(化学分册)》 CAS CSCD 北大核心 2007年第1期21-22,25,共3页
应用火焰原子吸收光谱法(FAAS)测定了红车轴草及其提取物中铁和锰的含量,试验了仪器的工作条件并予以优化,经干燥和粉碎的试样于瓷坩埚中用浓硝酸处理,经蒸发至干,于马弗炉中在500℃灼烧1 h重复处理一次后用0.2 mol·L^-1HCl浸取... 应用火焰原子吸收光谱法(FAAS)测定了红车轴草及其提取物中铁和锰的含量,试验了仪器的工作条件并予以优化,经干燥和粉碎的试样于瓷坩埚中用浓硝酸处理,经蒸发至干,于马弗炉中在500℃灼烧1 h重复处理一次后用0.2 mol·L^-1HCl浸取坩埚中残渣,所得溶液进行FAAS测定,两元素的线性范围为0.50~4.00 mg·L^-1(铁)和0.20~3.00 mg.L^-1(锰),相关系数依次为0.999 6和0.999 0。根据标准曲线斜率算得两元素的测定灵敏度依次为0.028和0.011 mg·L^-1/1%。试验得铁的回收率为98.9%,锰的回收率为96.2%,平行5次测定一红车轴草试样中铁及锰量,对平均含铁量为67.0μg.g^-1的相对标准偏差(RSD)值为2.45%,平均含锰量为7.8μg.g^-1的RSD值为2.10%。 展开更多
关键词 火焰原子吸收光谱法 红车轴草
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Genetic Diversity of Clover by SRAP
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作者 Runfang LI Hanfeng DING +2 位作者 Cune WANG Lingyun LU Xiaodong ZHANG 《Agricultural Biotechnology》 CAS 2019年第5期6-8,共3页
In the present study,genetic diversity and genetic relationship of 11materials including red clover ( Trifolium pretense L.),white clover ( T.repen L.) and alsike clover ( T.hybridum L.) and leaf-type variants of whit... In the present study,genetic diversity and genetic relationship of 11materials including red clover ( Trifolium pretense L.),white clover ( T.repen L.) and alsike clover ( T.hybridum L.) and leaf-type variants of white clover were investigated by SRAP (sequence related amplified polymorphism).Forty SRAP primers were screened,and 792 reliable bands were amplified,among which 426 (53.8%) were polymorphic.The number of polymorphic bands per pair of primers ranged from 3 to 38 with an average of 10.6.SRAP cluster analysis showed that the similarity coefficients between white clover materials were from 0.465 to 0.997 with an average of 0.812,indicating that there was certain genetic diversity among white clover.Specific bands appeared in white clover variants,indicating that there were certain differences in DNA sequence between normal while clover plants and their leaf-type variants.The similarity coefficients between red clover materials were from 0.457 to 0.827 with an average of 0.597,indicating that the different red clover germplasms had genetic diversity at the DNA molecular level.The results also showed that the genetic relationship of the alsike clover with the red clover was closer than that with the white clover.This study shows that SRAP technique can be effectively used for the analysis on interspecific and intraspecific relationship,germplasm resource identification and genetic diversity of clover. 展开更多
关键词 WHITE CLOVER ( trifolium repen L.) RED CLOVER ( trifolium pratense L.) Alsike CLOVER ( trifolium hybridum L.) SRAP
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Phenolic Profiles and Polyphenol Oxidase (PPO) Gene Expression of Red Clover (Trifolium pratense) Selected for Decreased Postharvest Browning 被引量:1
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作者 Isabelle A. Kagan Randy D. Dinkins Norman L. Taylor 《American Journal of Plant Sciences》 2016年第10期1478-1489,共12页
Red clover (Trifolium pratense L.) is a legume forage abundant in phenolic compounds. It tends to brown when cut for hay, due to oxidation of phenolic compounds catalyzed by polyphenol oxidase (PPO), and subsequent bi... Red clover (Trifolium pratense L.) is a legume forage abundant in phenolic compounds. It tends to brown when cut for hay, due to oxidation of phenolic compounds catalyzed by polyphenol oxidase (PPO), and subsequent binding to proteins. Selecting for a greener hay may provide information about the relationship of browning, PPO, and phenolics to each other. The red clover Kenland cultivar was selected over eight breeding cycles for decreased browning after being cut and dried 48 h in the field. Expression of PPO1 and PPO3, in Kenland and three of the eight cycles, was compared by real-time quantitative PCR. Phenolic compounds in Kenland and Cycle 8, collected 0, 24, and 48 h after cutting, were quantified by high-performance liquid chromatography (HPLC). Visual browning scores decreased 12% between Kenland and Cycle 8 (P = 0.02). PPO1 and PPO3 gene expression were not affected by selection. Clovamide decreased 26% in Cycle 8 relative to Kenland (P = 0.016). Sissotrin decreased 10% in Cycle 8 (P = 0.043). Neither total formononetin nor total biochanin A was affected by selection (P = 0.63 and 0.45, respectively). These results suggest that when selecting clover for decreased postharvest browning, a decrease occurs in a phenolic compound that can bind protein independently of PPO. However, PPO1 and PPO3 gene expression, and the major red clover isoflavones, are minimally affected. 展开更多
关键词 trifolium pratense L. BROWNING PPO Clovamide ISOFLAVONE HPLC
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Root Exudation of Organic Acids of Herbaceous Pioneer Plants and Their Growth in Sterile and Non-Sterile Nutrient-Poor,Sandy Soils from Post-Mining Sites 被引量:1
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作者 Katja BOLDT-BURISCH Bernd Uwe SCHNEIDER +1 位作者 M.Anne NAETH Reinhard F.HTTL 《Pedosphere》 SCIE CAS CSCD 2019年第1期34-44,共11页
Nutrient-poor, sandy soils form the prevailing substrate at post-mining sites of the Lusatian region(Brandenburg, Germany) and present a challenge for vegetation development. We studied the organic acid quantity and c... Nutrient-poor, sandy soils form the prevailing substrate at post-mining sites of the Lusatian region(Brandenburg, Germany) and present a challenge for vegetation development. We studied the organic acid quantity and composition of three commonly occurring pioneer plant species, the legumes Lotus corniculatus L. and Trifolium arvense L. and the grass Calamagrostis epigeios(L.) Roth, to determine if plant growth and exudation differed with(non-sterilized soil) and without(sterilized soil) an indigenous soil microbial community. We investigated whether organic acids were found in the rhizosphere and surrounding soil and whether this influenced nutrient mobilization. This study consists of linked field investigations and a greenhouse experiment. Plants were grown in the greenhouse in either sterilized or non-sterilized sandy soil from a reclamation site in the Lusatian mining landscape(Welzow Su¨d, East Germany). After seven months, the plant biomass, root morphology, organic acids, and water-soluble nutrients and root colonization with arbuscular mycorrhizal fungi(AMF) and dark septate endophytes(DSE) were analyzed. Roots of all three plants in the field and greenhouse experiments were highly colonized with AMF. Calamagrostis epigeios and T. arvense had a significantly higher colonization frequency with DSE than L. corniculatus. The quantity and composition of organic acids strongly differed among plant species, with the highest number of organic acids found for L. corniculatus and lowest for C. epigeios. The quantity of organic acids was greatly reduced in all plants under sterilized soil conditions. However, the composition of organic acids and plant growth in sterilized soil were reduced for both legumes, but not for C. epigeios, which had a higher biomass under sterilized conditions. Changes in nutrient concentrations in the field rhizosphere soil relative to those in the control were measurable after seven months. While the spectrum of organic acids and the growth of legumes seemed to be dependent on a highly 展开更多
关键词 Calamagrostis epigeios indigenous soil MICROBIAL community Lotus corniculatus MICROBIAL colonization mining landscape NUTRIENT MOBILIZATION root SYMBIOTIC partner trifolium arvense
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