Tea is the world＇s oldest and most popular caffeine-containing beverage with immense economic, medicinal, and cultural importance. Here, we present the first high-quality nucleotide sequence of the repeat-rich （80.9...Tea is the world＇s oldest and most popular caffeine-containing beverage with immense economic, medicinal, and cultural importance. Here, we present the first high-quality nucleotide sequence of the repeat-rich （80.9%）, 3.02-Gb genome of the cultivated tea tree Camellia sinensis. We show that an extraordinarily large genome size of tea tree is resulted from the slow, steady, and long-term amplification of a few LTR retrotransposon families. In addition to a recent whole-genome duplication event, lineage-specific expansions of genes associated with flavonoid metabolic biosynthesis were discovered, which enhance catechin production, terpene enzyme activation, and stress tolerance, important features for tea flavor and adaptation. We demonstrate an independent and rapid evolution of the tea caffeine synthesis pathway relative to cacao and coffee. A comparative study among 25 Camellia species revealed that higher expression levels of most flavonoid- and caffeinebut not theanine-related genes contribute to the increased production of catechins and caffeine and thus enhance tea-processing suitability and tea quality. These novel findings pave the way for further metabolomic and functional genomic refinement of characteristic biosynthesis pathways and will help develop a more diversified set of tea flavors that would eventually satisfy and attract more tea drinkers worldwide.展开更多
By combining living trees and archaeological wood, the annual mean temperatures were reconstructed based on ring-width indices of the mid-eastern Tibetan Plateau for the past 2485 years. The climate variations reveale...By combining living trees and archaeological wood, the annual mean temperatures were reconstructed based on ring-width indices of the mid-eastern Tibetan Plateau for the past 2485 years. The climate variations revealed by the reconstruction indicate that there were four periods to have average tem- peratures similar to or even higher than that mean of 1970 to 2000 AD. A particularly notable rapid shift from cold to warm, we call it the "Eastern Jin Event", occurred from 348 AD to 413 AD. Calculation re- sults show that the temperature variations over the mid-eastern Tibetan Plateau are not only repre- sentative for large parts of north-central China, but also closely correspond to those of the entire Northern Hemisphere over long time scales. During the last 2485 years, the downfall of most major dynasties in China coincides with intervals of low temperature. Compared with the temperature records in other regions of China during the last 1000 years, this reconstruction from the Tibetan Plateau shows a significant warming trend after the 1950s.展开更多
Land surface temperature （LST）, which is heavily influenced by urban surface structures, is a significant parameter in urban environmental analysis. This study examined the effect impervious surfaces （IS） spatial ...Land surface temperature （LST）, which is heavily influenced by urban surface structures, is a significant parameter in urban environmental analysis. This study examined the effect impervious surfaces （IS） spatial patterns have on LST in Beijing, China. A classification and regression tree model （CART） was adopted to estimate IS as a continuous variable using Landsat images from two seasons combined with QuickBird. LST was retrieved from the Landsat Thematic Mapper （TM） image to examine the relationships between IS and LST. The results revealed that CART was capable of consistently predicting LST with acceptable accuracy （correlation coefficient of 0.94 and the average error of 8.59%）. Spatial patterns of IS exhibited changing gradients across the various urban-rural transects, with LST values showing a concentric shape that increased as you moved from the outskirts towards the downtown areas. Transect analysis also indicated that the changes in both IS and LST patterns were similar at various resolution levels, which suggests a distinct linear relationship between them. Results of correlation analysis further showed that IS tended to be positively correlated with LST, and that the correlation coefficients increased from 0.807 to 0.925 with increases in IS pixel size. The findings identified in this study provide a theoretical basis for improving urban planning efforts to lessen urban temperatures and thus dampen urban heat island effects.展开更多
基金This work was supported by the project of Yunnan Innovation Team Project, the Hundreds Oversea Talents Program of Yunnan Province, the Top Talents Program of Yunnan Province （Grant 20080A009）, the Key Project of the Natural Science Foundation of Yunnan Province （201401 PC00397）, National Science Foundation of China （U0936603）, Key Project of Natural Science Foundation of Yunnan Province （2008CC016）, Frontier Grant of Kunming Institute of Botany, CAS （672705232515）, Top Talents Program of Yunnan Province （20080A009）, and Hundreds Talents Program of Chinese Academy of Sciences （CAS） （to L.G.）.
文摘Tea is the world＇s oldest and most popular caffeine-containing beverage with immense economic, medicinal, and cultural importance. Here, we present the first high-quality nucleotide sequence of the repeat-rich （80.9%）, 3.02-Gb genome of the cultivated tea tree Camellia sinensis. We show that an extraordinarily large genome size of tea tree is resulted from the slow, steady, and long-term amplification of a few LTR retrotransposon families. In addition to a recent whole-genome duplication event, lineage-specific expansions of genes associated with flavonoid metabolic biosynthesis were discovered, which enhance catechin production, terpene enzyme activation, and stress tolerance, important features for tea flavor and adaptation. We demonstrate an independent and rapid evolution of the tea caffeine synthesis pathway relative to cacao and coffee. A comparative study among 25 Camellia species revealed that higher expression levels of most flavonoid- and caffeinebut not theanine-related genes contribute to the increased production of catechins and caffeine and thus enhance tea-processing suitability and tea quality. These novel findings pave the way for further metabolomic and functional genomic refinement of characteristic biosynthesis pathways and will help develop a more diversified set of tea flavors that would eventually satisfy and attract more tea drinkers worldwide.
基金Supported by National Natural Science Foundation of China (Grant Nos. 40525004, 40599420, 40890051)National Basic Research Program of China (Grant Nos. 2007BAC30B00, 2004CB720200, 2006CB400503)the Swedish International Development Cooperation Agency (SIDA, Grant to Hans W. Linderholm)
文摘By combining living trees and archaeological wood, the annual mean temperatures were reconstructed based on ring-width indices of the mid-eastern Tibetan Plateau for the past 2485 years. The climate variations revealed by the reconstruction indicate that there were four periods to have average tem- peratures similar to or even higher than that mean of 1970 to 2000 AD. A particularly notable rapid shift from cold to warm, we call it the "Eastern Jin Event", occurred from 348 AD to 413 AD. Calculation re- sults show that the temperature variations over the mid-eastern Tibetan Plateau are not only repre- sentative for large parts of north-central China, but also closely correspond to those of the entire Northern Hemisphere over long time scales. During the last 2485 years, the downfall of most major dynasties in China coincides with intervals of low temperature. Compared with the temperature records in other regions of China during the last 1000 years, this reconstruction from the Tibetan Plateau shows a significant warming trend after the 1950s.
基金Project supported by the Pilot Project of Knowledge Innovadon Program of Chinese Academy of Sciences (No. KZCX32SW2424).
文摘Land surface temperature （LST）, which is heavily influenced by urban surface structures, is a significant parameter in urban environmental analysis. This study examined the effect impervious surfaces （IS） spatial patterns have on LST in Beijing, China. A classification and regression tree model （CART） was adopted to estimate IS as a continuous variable using Landsat images from two seasons combined with QuickBird. LST was retrieved from the Landsat Thematic Mapper （TM） image to examine the relationships between IS and LST. The results revealed that CART was capable of consistently predicting LST with acceptable accuracy （correlation coefficient of 0.94 and the average error of 8.59%）. Spatial patterns of IS exhibited changing gradients across the various urban-rural transects, with LST values showing a concentric shape that increased as you moved from the outskirts towards the downtown areas. Transect analysis also indicated that the changes in both IS and LST patterns were similar at various resolution levels, which suggests a distinct linear relationship between them. Results of correlation analysis further showed that IS tended to be positively correlated with LST, and that the correlation coefficients increased from 0.807 to 0.925 with increases in IS pixel size. The findings identified in this study provide a theoretical basis for improving urban planning efforts to lessen urban temperatures and thus dampen urban heat island effects.
