AIM： TO screen the differential expressed genes in colorectal cancer and polyp tissue samples. METHODS： Tissue specimens containing 16 cases of colorectal adenocarcinoma and colorectal polyp vs nor- mal mucosae were...AIM： TO screen the differential expressed genes in colorectal cancer and polyp tissue samples. METHODS： Tissue specimens containing 16 cases of colorectal adenocarcinoma and colorectal polyp vs nor- mal mucosae were collected and subjected to cDNA microarray and bioinformatical analyses. Quantitative reverse transcription-polymerase chain reaction （qRT- PCR） was used to confirm some of the cDNA microarray data.RESULTS： The experimental data showed that eight genes were differentially expressed, most of which were upregulated in adenomatous polyp lesions. Forty-six genes expressions were altered in colorectal cancers, of which 29 were upregulated and 17 downregulated, as compared to the normal mucosae. In addition, 18 genes were similarly altered in both adenomatous polyps and colorectal cancer, qRT-PCR analyses confirmed the cDNA microarray data for four of those 18 genes： MTA1, PDCD4, TSC1 and PDGFRA. CONCLUSION： These differentially expressed genes likely represent biomarkers for early detection of co- Iorectal cancer and may be potential therapeutic targets after confirmed by further studies.展开更多
The B-cell lymphoma/leukemia 11A （BCL11A） gene is essential for normal lymphoid development and has been associated with hematological malignancies. In the current study, the relative expression level of BCL11A in m...The B-cell lymphoma/leukemia 11A （BCL11A） gene is essential for normal lymphoid development and has been associated with hematological malignancies. In the current study, the relative expression level of BCL11A in malignant hematological cell lines was evaluated through real-time quantitative reverse transcription polymerase chain reaction （qRT-PCR）. METHODS The relative expression level of BCLllA mRNA in malignant hematological cell lines was determined through qRT- PCR using SYBR Green I dye. Glyceraldehyde-3-phosphate dehydro- genase was used as the reference gene to confirm the relative expression level of BCL11A gene mRNA. RESULTS The relative expression level of BCL11A mRNA in cell lines from B-cell malignancies was significantly higher compared with that from acute rnyeloid leukemia （P 〈 0.05）. Different cell lines with malignant B-cells exhibited a wide range of BCL11A expressions ranging from 27.37 to 93.38. CONCLUSION The overexpression of BCL11A gene mRNA in malignant B-cells might play a role in B-cell lymphoma/leukemia.展开更多
目的探讨牙龈组织中是否存在β防御素-4(hBD-4)及其分布情况。方法收集健康牙龈标本。采用免疫组织化学法检测18例牙龈组织标本hBD-4蛋白水平的分布;提取组织RNA,进行实时荧光定量逆转录聚合酶链反应(real time RT-PCR)检测30例牙龈标本...目的探讨牙龈组织中是否存在β防御素-4(hBD-4)及其分布情况。方法收集健康牙龈标本。采用免疫组织化学法检测18例牙龈组织标本hBD-4蛋白水平的分布;提取组织RNA,进行实时荧光定量逆转录聚合酶链反应(real time RT-PCR)检测30例牙龈标本中hBD-4基因的表达水平。结果免疫组织化学检测结果显示,18例牙龈样本中有13例检出hBD-4;real time RT-PCR检测结果显示,30例牙龈组织中4例样本检测出hBD-4表达。结论hBD-4在健康牙龈组织中有不同水平的表达和分布,可能在维持牙周组织健康中起作用。展开更多
基金Supported by Grant from Medical Technology Innovation Project of Nanjing Military,No. 09MA066
文摘AIM： TO screen the differential expressed genes in colorectal cancer and polyp tissue samples. METHODS： Tissue specimens containing 16 cases of colorectal adenocarcinoma and colorectal polyp vs nor- mal mucosae were collected and subjected to cDNA microarray and bioinformatical analyses. Quantitative reverse transcription-polymerase chain reaction （qRT- PCR） was used to confirm some of the cDNA microarray data.RESULTS： The experimental data showed that eight genes were differentially expressed, most of which were upregulated in adenomatous polyp lesions. Forty-six genes expressions were altered in colorectal cancers, of which 29 were upregulated and 17 downregulated, as compared to the normal mucosae. In addition, 18 genes were similarly altered in both adenomatous polyps and colorectal cancer, qRT-PCR analyses confirmed the cDNA microarray data for four of those 18 genes： MTA1, PDCD4, TSC1 and PDGFRA. CONCLUSION： These differentially expressed genes likely represent biomarkers for early detection of co- Iorectal cancer and may be potential therapeutic targets after confirmed by further studies.
文摘The B-cell lymphoma/leukemia 11A （BCL11A） gene is essential for normal lymphoid development and has been associated with hematological malignancies. In the current study, the relative expression level of BCL11A in malignant hematological cell lines was evaluated through real-time quantitative reverse transcription polymerase chain reaction （qRT-PCR）. METHODS The relative expression level of BCLllA mRNA in malignant hematological cell lines was determined through qRT- PCR using SYBR Green I dye. Glyceraldehyde-3-phosphate dehydro- genase was used as the reference gene to confirm the relative expression level of BCL11A gene mRNA. RESULTS The relative expression level of BCL11A mRNA in cell lines from B-cell malignancies was significantly higher compared with that from acute rnyeloid leukemia （P 〈 0.05）. Different cell lines with malignant B-cells exhibited a wide range of BCL11A expressions ranging from 27.37 to 93.38. CONCLUSION The overexpression of BCL11A gene mRNA in malignant B-cells might play a role in B-cell lymphoma/leukemia.
文摘目的探讨牙龈组织中是否存在β防御素-4(hBD-4)及其分布情况。方法收集健康牙龈标本。采用免疫组织化学法检测18例牙龈组织标本hBD-4蛋白水平的分布;提取组织RNA,进行实时荧光定量逆转录聚合酶链反应(real time RT-PCR)检测30例牙龈标本中hBD-4基因的表达水平。结果免疫组织化学检测结果显示,18例牙龈样本中有13例检出hBD-4;real time RT-PCR检测结果显示,30例牙龈组织中4例样本检测出hBD-4表达。结论hBD-4在健康牙龈组织中有不同水平的表达和分布,可能在维持牙周组织健康中起作用。
基金supported by the National Natural Science Foundation of China(Nos.30871295)the National Ministry of Personnel Scientific Research Foundation of student(No.[2001]33)+1 种基金the Science Research of the Science Committee of Chongqing(No.CSTC 2009BB5065)
