建立了固相萃取-高效液相色谱法同时测定植物根际促生菌发酵产物中吲哚-3-乙酸、赤霉素和玉米素3种植物激素含量的方法。固相萃取采用Agilent Bond Elut C18固相萃取柱,6 mL甲醇+6 mL 10%甲醇活化;以5 mL 10%甲醇淋洗2次;以5mL 80%甲醇...建立了固相萃取-高效液相色谱法同时测定植物根际促生菌发酵产物中吲哚-3-乙酸、赤霉素和玉米素3种植物激素含量的方法。固相萃取采用Agilent Bond Elut C18固相萃取柱,6 mL甲醇+6 mL 10%甲醇活化;以5 mL 10%甲醇淋洗2次;以5mL 80%甲醇洗脱2次。采用Agilent ZORBAX Eclipse Plus C18色谱柱(250×4.6mm,5μm)分离,流动相采用体积比为2∶3的甲醇-水(含0.2%冰乙酸)二元混合溶剂,流速为1mL·min^(-1),柱温为30℃,进样量为20μL,检测波长为210nm。结果表明:10株供试菌株发酵产物中均能检测到3种植物激素,除菌株003NXZ4外,其余菌株均呈现出玉米素含量最高,赤霉素次之,吲哚-3-乙酸含量最低的趋势。3种植物激素在12min内分离完全,样品的加标回收率达90.3%~104.3%,相对标准偏差(n=6)在1.04%~2.98%之间。该方法简便、准确,可同时检测植物根际促生菌发酵产物中吲哚-3-乙酸、赤霉素和玉米素3种植物激素含量。展开更多
The cytokinin oxidase/dehydrogenase(CKX)enzyme is essential for controlling thefluctuating levels of endogen-ous cytokinin(CK)and has a significant impact on different aspects of plant growth and development.Nonethe-les...The cytokinin oxidase/dehydrogenase(CKX)enzyme is essential for controlling thefluctuating levels of endogen-ous cytokinin(CK)and has a significant impact on different aspects of plant growth and development.Nonethe-less,there is limited knowledge about CKX genes in tomato(Solanum lycopersicum L.).Here we performed genome-wide identification and analysis of nine SlCKX family members in tomatoes using bioinformatics tools.The results revealed that nine SlCKX genes were unevenly distributed onfive chromosomes(Chr.1,Chr.4,Chr.8,Chr.10,and Chr.12).The amino acid length,isoelectric points,and molecular weight of the nine SlCKX proteins ranged from 453 to 553,5.77 to 8.59,and 51.661 to 62.494 kD,respectively.Subcellular localization analysis indi-cated that SlCKX2 proteins were located in both the vacuole and cytoplasmic matrix;SlCKX3 and SlCKX5 pro-teins were located in the vacuole;and SlCKX1,4,6,7,8,and 9 proteins were located in the cytoplasmic matrix.Furthermore,we observed differences in the gene structures and phylogenetic relationships of SlCKX proteins among different members.SlCKX1-9 were positioned on two out of the three branches of the CKX phylogenetic tree in the multispecies phylogenetic tree construction,revealing their strong conservation within phylogenetic subgroups.Unique patterns of expression of CKX genes were noticed in callus cultures exposed to varying con-centrations of exogenous ZT,suggesting their roles in specific developmental and physiological functions in the regeneration system.These results may facilitate subsequent functional analysis of SlCKX genes and provide valu-able insights for establishing an efficient regeneration system for tomatoes.展开更多
This review chronicles the development of the cytokinin research during the last 30 years. Cytokinin and auxin are the two major plant growth hormones that control virtually all aspects of growth and development in hi...This review chronicles the development of the cytokinin research during the last 30 years. Cytokinin and auxin are the two major plant growth hormones that control virtually all aspects of growth and development in higher plants. The pathways for cytokinin biosynthesis and metabolism have been characterized by the identification of isopentenyl pyrophosphate transferase, cytokinin oxidases, cytokinin hydroxylase, zeatin cis-/trans-isomerase, cytokinin phosphoribosyl hydrolases, cytokinin-specific riboside phosphorylase, and others enzymes. Loss-of function mutant phenotypes of cytokinin degradation/activating enzymes indicate the regulation of concentration and spatial distribution of bio-active cytokinin plays a pivotal role in the increase in panicle size, in the numbers of floral organs, and eventually in seed yield. One of the most fundamental questions in the cytokinin field is one concerning the prevalence of cis-zeatin in monocotyledonous crops (rice and maize) and in dicotyledonous legumes (pea, chickpea) and potato/sweet potato. A hypothesis is that cis-zeatin is synthesized by the cis-specific hydroxylation of the terminal methyl group of N6-isopentenyl side chain of N6-isopentenyl adenosine (i6Ado) or of their mono-, di-, or tri-phosphates catalyzed by the cis-specific hydroxylase. A second potential pathway is the isomerization of trans-zeatin to cis-zeatin by zeatin cis-/trans-isomerase. A second fundamental question to be addressed is the physiological role of cis-zeatin. Some have argued for a special function of cis-zeatin to account for the prevalence of the cis-zeatin in the plant kingdom from algae to higher plants.展开更多
文摘建立了固相萃取-高效液相色谱法同时测定植物根际促生菌发酵产物中吲哚-3-乙酸、赤霉素和玉米素3种植物激素含量的方法。固相萃取采用Agilent Bond Elut C18固相萃取柱,6 mL甲醇+6 mL 10%甲醇活化;以5 mL 10%甲醇淋洗2次;以5mL 80%甲醇洗脱2次。采用Agilent ZORBAX Eclipse Plus C18色谱柱(250×4.6mm,5μm)分离,流动相采用体积比为2∶3的甲醇-水(含0.2%冰乙酸)二元混合溶剂,流速为1mL·min^(-1),柱温为30℃,进样量为20μL,检测波长为210nm。结果表明:10株供试菌株发酵产物中均能检测到3种植物激素,除菌株003NXZ4外,其余菌株均呈现出玉米素含量最高,赤霉素次之,吲哚-3-乙酸含量最低的趋势。3种植物激素在12min内分离完全,样品的加标回收率达90.3%~104.3%,相对标准偏差(n=6)在1.04%~2.98%之间。该方法简便、准确,可同时检测植物根际促生菌发酵产物中吲哚-3-乙酸、赤霉素和玉米素3种植物激素含量。
基金funded by the Special Project for Science and Technology Innovation Platform of Fujian Academy of Agricultural Sciences,China(CXPT2023003)the Freely Explore Scientific and Technology Innovation Program of Fujian Academy of Agricultural Sciences(ZYTS202207)the Program for Innovative Research Team of Fujian Academy of Agricultural Sciences,China(CXTD2021006-3)。
文摘The cytokinin oxidase/dehydrogenase(CKX)enzyme is essential for controlling thefluctuating levels of endogen-ous cytokinin(CK)and has a significant impact on different aspects of plant growth and development.Nonethe-less,there is limited knowledge about CKX genes in tomato(Solanum lycopersicum L.).Here we performed genome-wide identification and analysis of nine SlCKX family members in tomatoes using bioinformatics tools.The results revealed that nine SlCKX genes were unevenly distributed onfive chromosomes(Chr.1,Chr.4,Chr.8,Chr.10,and Chr.12).The amino acid length,isoelectric points,and molecular weight of the nine SlCKX proteins ranged from 453 to 553,5.77 to 8.59,and 51.661 to 62.494 kD,respectively.Subcellular localization analysis indi-cated that SlCKX2 proteins were located in both the vacuole and cytoplasmic matrix;SlCKX3 and SlCKX5 pro-teins were located in the vacuole;and SlCKX1,4,6,7,8,and 9 proteins were located in the cytoplasmic matrix.Furthermore,we observed differences in the gene structures and phylogenetic relationships of SlCKX proteins among different members.SlCKX1-9 were positioned on two out of the three branches of the CKX phylogenetic tree in the multispecies phylogenetic tree construction,revealing their strong conservation within phylogenetic subgroups.Unique patterns of expression of CKX genes were noticed in callus cultures exposed to varying con-centrations of exogenous ZT,suggesting their roles in specific developmental and physiological functions in the regeneration system.These results may facilitate subsequent functional analysis of SlCKX genes and provide valu-able insights for establishing an efficient regeneration system for tomatoes.
文摘This review chronicles the development of the cytokinin research during the last 30 years. Cytokinin and auxin are the two major plant growth hormones that control virtually all aspects of growth and development in higher plants. The pathways for cytokinin biosynthesis and metabolism have been characterized by the identification of isopentenyl pyrophosphate transferase, cytokinin oxidases, cytokinin hydroxylase, zeatin cis-/trans-isomerase, cytokinin phosphoribosyl hydrolases, cytokinin-specific riboside phosphorylase, and others enzymes. Loss-of function mutant phenotypes of cytokinin degradation/activating enzymes indicate the regulation of concentration and spatial distribution of bio-active cytokinin plays a pivotal role in the increase in panicle size, in the numbers of floral organs, and eventually in seed yield. One of the most fundamental questions in the cytokinin field is one concerning the prevalence of cis-zeatin in monocotyledonous crops (rice and maize) and in dicotyledonous legumes (pea, chickpea) and potato/sweet potato. A hypothesis is that cis-zeatin is synthesized by the cis-specific hydroxylation of the terminal methyl group of N6-isopentenyl side chain of N6-isopentenyl adenosine (i6Ado) or of their mono-, di-, or tri-phosphates catalyzed by the cis-specific hydroxylase. A second potential pathway is the isomerization of trans-zeatin to cis-zeatin by zeatin cis-/trans-isomerase. A second fundamental question to be addressed is the physiological role of cis-zeatin. Some have argued for a special function of cis-zeatin to account for the prevalence of the cis-zeatin in the plant kingdom from algae to higher plants.
