JWA, a cytoskeleton associated gene, was primarily found to be regulated by all trans-retinoic acid (ATRA), 13 cis-retinoic acid (13 cis-RA) and 12-tetradecano-ylphorbol-13-acetate (TPA). Our previous data showed that...JWA, a cytoskeleton associated gene, was primarily found to be regulated by all trans-retinoic acid (ATRA), 13 cis-retinoic acid (13 cis-RA) and 12-tetradecano-ylphorbol-13-acetate (TPA). Our previous data showed that JWA might be involved in both cellular differentiation and apoptosis induced by several chemicals. In this study, we addressed the possible mechanism of JWA in the regulation of cell differentiation and apoptosis in NB4, a human acute promyelocytic leukemia cell line. CD11b/CD33 expression and cell cycle were analyzed for detecting of cell differentiation and apoptosis. Both reverse-transcription polymerase chain reaction (RT-PCR) and Western blot assays were used for understanding the expressions of JWA. The results showed that under the indicated concentrations ATRA (10-6 mol/L) and As2O3 (10-6 mol/L) induced cell differentiation and apoptosis separately; while both 4HPR (10-6mol/L) and TPA (10-7 mol/L) showed dual-directional effects on NB4 cells, they not only trigger cells’展开更多
Two-component signal transduction systems(TCSTSs),consisting of a histidine kinase and a response regulator,play a critical role in regulating virulence gene expression in Gram-negative phytopathogenic bacteria Xantho...Two-component signal transduction systems(TCSTSs),consisting of a histidine kinase and a response regulator,play a critical role in regulating virulence gene expression in Gram-negative phytopathogenic bacteria Xanthomonas spp..To date,12 TCSTS genes have been identified,accounting for approximately 10%of the TCSTS genes in each genome that have been experimen-tally identified to be related to pathogenesis.These TCSTSs modulate the expression of a number of virulence factors through diverse molecular mechanisms such as interacting with DNA,protein-binding and involvement in second messenger metabolism,which generates a high level of regulatory versatility.Here we summarize the current knowledge in thisfield and discuss the emerging themes and remaining questions that are important in deciphering the signaling network of TCSTSs in Xantho-monas.展开更多
Receptor-like kinases(RLKs)are a large group of plant-specific transmembrane proteins mainly acting as receptors or co-receptors of various extracellular signals.They usually turn extracellular signals into intracellu...Receptor-like kinases(RLKs)are a large group of plant-specific transmembrane proteins mainly acting as receptors or co-receptors of various extracellular signals.They usually turn extracellular signals into intracellular responses via altering gene expression profiles.However,recent studies confirmed that many RLKs can physically interact with diverse membrane-localized transport proteins and regulate their activities for speedy responses in limited tissues or cells.In this minireview,we highlight recent discoveries regarding how RLKs can work with membrane transport proteins collaboratively and thereby trigger cellular responses in a precise and rapid manner.It is anticipated that such regulation broadly presents in plants and more examples will be gradually revealed when in-depth analyses are conducted for the functions of RLKs.展开更多
基金This work was supported in part by the National Natural Science Foundation of China (Grant Nos. 30070664 and 30170812)the Natural Science Foundation of Jiangsu Province (Grant No. BK99133) the Science Foundation of Nanjing Medical University (Grant N
文摘JWA, a cytoskeleton associated gene, was primarily found to be regulated by all trans-retinoic acid (ATRA), 13 cis-retinoic acid (13 cis-RA) and 12-tetradecano-ylphorbol-13-acetate (TPA). Our previous data showed that JWA might be involved in both cellular differentiation and apoptosis induced by several chemicals. In this study, we addressed the possible mechanism of JWA in the regulation of cell differentiation and apoptosis in NB4, a human acute promyelocytic leukemia cell line. CD11b/CD33 expression and cell cycle were analyzed for detecting of cell differentiation and apoptosis. Both reverse-transcription polymerase chain reaction (RT-PCR) and Western blot assays were used for understanding the expressions of JWA. The results showed that under the indicated concentrations ATRA (10-6 mol/L) and As2O3 (10-6 mol/L) induced cell differentiation and apoptosis separately; while both 4HPR (10-6mol/L) and TPA (10-7 mol/L) showed dual-directional effects on NB4 cells, they not only trigger cells’
基金supported by the National Natural Science Foundation of China(Grant No.30771401)The National Basic Research Program(No.2011CB100700)from the Ministry of Science and Technology of China.
文摘Two-component signal transduction systems(TCSTSs),consisting of a histidine kinase and a response regulator,play a critical role in regulating virulence gene expression in Gram-negative phytopathogenic bacteria Xanthomonas spp..To date,12 TCSTS genes have been identified,accounting for approximately 10%of the TCSTS genes in each genome that have been experimen-tally identified to be related to pathogenesis.These TCSTSs modulate the expression of a number of virulence factors through diverse molecular mechanisms such as interacting with DNA,protein-binding and involvement in second messenger metabolism,which generates a high level of regulatory versatility.Here we summarize the current knowledge in thisfield and discuss the emerging themes and remaining questions that are important in deciphering the signaling network of TCSTSs in Xantho-monas.
基金The research conducted in J.L.lab is currently supported by the National Natural Science Foundation of China(31720103902 and 32030005 to J.L.)111 Project(B16022 to J.L.)China Postdoctoral Science Foundation(2020M683606 to X.L.)。
文摘Receptor-like kinases(RLKs)are a large group of plant-specific transmembrane proteins mainly acting as receptors or co-receptors of various extracellular signals.They usually turn extracellular signals into intracellular responses via altering gene expression profiles.However,recent studies confirmed that many RLKs can physically interact with diverse membrane-localized transport proteins and regulate their activities for speedy responses in limited tissues or cells.In this minireview,we highlight recent discoveries regarding how RLKs can work with membrane transport proteins collaboratively and thereby trigger cellular responses in a precise and rapid manner.It is anticipated that such regulation broadly presents in plants and more examples will be gradually revealed when in-depth analyses are conducted for the functions of RLKs.
