目的研究优化糙叶五加叶总黄酮的大孔吸附树脂纯化工艺。方法以处理后的总黄酮的质量分数和总黄酮得率为考察指标,采用单因素实验结合响应曲面设计法进行纯化工艺优化研究。结果 D101型大孔吸附树脂对糙叶五加叶总黄酮有较好的吸附和洗...目的研究优化糙叶五加叶总黄酮的大孔吸附树脂纯化工艺。方法以处理后的总黄酮的质量分数和总黄酮得率为考察指标,采用单因素实验结合响应曲面设计法进行纯化工艺优化研究。结果 D101型大孔吸附树脂对糙叶五加叶总黄酮有较好的吸附和洗脱效果,其最佳的纯化工艺条件为色谱柱的径高比为1∶10,25.0 g D101大孔树脂的上样量为750 mg,溶剂体系为50%乙醇,洗脱体积流量为5 m L/min,洗脱体积为130 m L。在此最佳优化条件下,糙叶五加叶总黄酮的理论质量分数为75.69%,实际质量分数达到75.87%;总黄酮得率为30.13%。结论 D101型大孔吸附树脂宜于纯化糙叶五加叶中的总黄酮,且响应面法能有效地优化其纯化工艺。展开更多
[Objectives] To optimize the extraction of total flavone in folium Ginkgo biloba leaves.[Methods] Using the ultrasonic extraction method, the three levels of maximum impact were selected by each factor selection, and ...[Objectives] To optimize the extraction of total flavone in folium Ginkgo biloba leaves.[Methods] Using the ultrasonic extraction method, the three levels of maximum impact were selected by each factor selection, and the optimal extraction technology was obtained by box-benhnken test design analysis.[Results] After analyzing the results, the optimal process was as follows: a time of 28.89 min and liquid/material ratio of 31.38∶ 1, the temperature was 40.74℃. In this way, NaNO 2-Al (NO 3) 3-NaOH was used as color developing agent, and the theoretical extraction of flavone in ginkgo leaf can reach 11.74 mg/g.[Conclusions] This method can be used to extract total flavonoids from G. biloba leaves with simple operation, low cost and good repeatability.展开更多
文摘目的研究优化糙叶五加叶总黄酮的大孔吸附树脂纯化工艺。方法以处理后的总黄酮的质量分数和总黄酮得率为考察指标,采用单因素实验结合响应曲面设计法进行纯化工艺优化研究。结果 D101型大孔吸附树脂对糙叶五加叶总黄酮有较好的吸附和洗脱效果,其最佳的纯化工艺条件为色谱柱的径高比为1∶10,25.0 g D101大孔树脂的上样量为750 mg,溶剂体系为50%乙醇,洗脱体积流量为5 m L/min,洗脱体积为130 m L。在此最佳优化条件下,糙叶五加叶总黄酮的理论质量分数为75.69%,实际质量分数达到75.87%;总黄酮得率为30.13%。结论 D101型大孔吸附树脂宜于纯化糙叶五加叶中的总黄酮,且响应面法能有效地优化其纯化工艺。
基金Supported by Natural Science Research Projects of Colleges and Universities in Anhui Province(KJ2019A1180,KJ2018A0884,KJ2017A772)
文摘[Objectives] To optimize the extraction of total flavone in folium Ginkgo biloba leaves.[Methods] Using the ultrasonic extraction method, the three levels of maximum impact were selected by each factor selection, and the optimal extraction technology was obtained by box-benhnken test design analysis.[Results] After analyzing the results, the optimal process was as follows: a time of 28.89 min and liquid/material ratio of 31.38∶ 1, the temperature was 40.74℃. In this way, NaNO 2-Al (NO 3) 3-NaOH was used as color developing agent, and the theoretical extraction of flavone in ginkgo leaf can reach 11.74 mg/g.[Conclusions] This method can be used to extract total flavonoids from G. biloba leaves with simple operation, low cost and good repeatability.
