The biochemical effects of gadolinium chloride were studied using high-resolution IH nuclear magnetic resonance （NMR） spec- troscopy to investigate the biochemical composition of tissue （liver and kidney） aqueous ...The biochemical effects of gadolinium chloride were studied using high-resolution IH nuclear magnetic resonance （NMR） spec- troscopy to investigate the biochemical composition of tissue （liver and kidney） aqueous extracts obtained from control and gadolinium chloride （GdCl3） （10 and 50 mg/kg body weight, intraperitoneal injection, i.p.） treated rats. Tissue samples were collected at 48, 96 and 168 h p.d. after exposure to GdCI3, and extracted using methanol/chloroform solvent system. ^1H NMR spectra of tissue extracts were analyzed by pat- tern recognition using principal components analysis. The liver damages caused by GdCl3 were characterized by increased succinate and decreased glycogen level and elevated lactate, alanine and betaine concentration in liver. Furthermore, the increase of creatine and lactate, and decrease of glutamate, alanine, phosphocholine, glycophosphocholine （GPC）, betaine, myo-inositol and trimethylamine N-oxide （TMAO） levels in kidney illustrated kidney disturbance induced by GdCl3.展开更多
To study the changes of thrombomodulin(TM) in both plasma and tissue extracts of cancer patients for evaluating its clinical significance. Methods: PlasmaTM levels were measured by enzyme-linked immunosorbent assay (E...To study the changes of thrombomodulin(TM) in both plasma and tissue extracts of cancer patients for evaluating its clinical significance. Methods: PlasmaTM levels were measured by enzyme-linked immunosorbent assay (ELISA) in both plasma of 188 cancer patients and 24 cancer tissue extractsincluding their adjacent non-cancer tissues. Results:The plasma TM levels both in cancer patients and in metastasis patients were significantly higher than that in controls [(33.4714.25)mg/L, (41.6816.96)mg/L, vs(20.40 7.22) mg/L,P<0.01]. The plasma TM levels incancer patients after operation decreased obviously thanthat before operation [(18.459.96)mg/L, vs (28.2911.74)mg/L, P<0.01], whereas, the plasma TM levels in patientswith recurrence and metastasis after operation increasedobviously [(34.5012.57)mg/L]. Among the types of cancer,the plasma TM levels in metastasis lung cancers, gastric cancers and pancreatic cancers were significantly higherthan that in non-metastasis respective cancers. Nosignificant differences were found between controls andnon-metastasis cancers including gastric cancers,pancreatic cancers, nasopharyngeal cancers, large intestine cancers and laryngeal cancers (P>0.05). The TM levels incancer tissue extracts were significantly lower than that intheir adjacent non-cancer tissue extracts [(647.71317.51)mg/L vs (1455.63772.22)mg/L, P<0.01]. On the contrary, the plasma TM levels in these cancers were significantly higher than that in controls. Conclusion: The rise of plasma TMlevels in cancer patients was associated with metastasis and diffusion of cancers. The TM levels can be served as ansensitive index for judging progression and metastasis of展开更多
基金supported by the National Natural Science Foundation of China (20575065,20637010)
文摘The biochemical effects of gadolinium chloride were studied using high-resolution IH nuclear magnetic resonance （NMR） spec- troscopy to investigate the biochemical composition of tissue （liver and kidney） aqueous extracts obtained from control and gadolinium chloride （GdCl3） （10 and 50 mg/kg body weight, intraperitoneal injection, i.p.） treated rats. Tissue samples were collected at 48, 96 and 168 h p.d. after exposure to GdCI3, and extracted using methanol/chloroform solvent system. ^1H NMR spectra of tissue extracts were analyzed by pat- tern recognition using principal components analysis. The liver damages caused by GdCl3 were characterized by increased succinate and decreased glycogen level and elevated lactate, alanine and betaine concentration in liver. Furthermore, the increase of creatine and lactate, and decrease of glutamate, alanine, phosphocholine, glycophosphocholine （GPC）, betaine, myo-inositol and trimethylamine N-oxide （TMAO） levels in kidney illustrated kidney disturbance induced by GdCl3.
文摘To study the changes of thrombomodulin(TM) in both plasma and tissue extracts of cancer patients for evaluating its clinical significance. Methods: PlasmaTM levels were measured by enzyme-linked immunosorbent assay (ELISA) in both plasma of 188 cancer patients and 24 cancer tissue extractsincluding their adjacent non-cancer tissues. Results:The plasma TM levels both in cancer patients and in metastasis patients were significantly higher than that in controls [(33.4714.25)mg/L, (41.6816.96)mg/L, vs(20.40 7.22) mg/L,P<0.01]. The plasma TM levels incancer patients after operation decreased obviously thanthat before operation [(18.459.96)mg/L, vs (28.2911.74)mg/L, P<0.01], whereas, the plasma TM levels in patientswith recurrence and metastasis after operation increasedobviously [(34.5012.57)mg/L]. Among the types of cancer,the plasma TM levels in metastasis lung cancers, gastric cancers and pancreatic cancers were significantly higherthan that in non-metastasis respective cancers. Nosignificant differences were found between controls andnon-metastasis cancers including gastric cancers,pancreatic cancers, nasopharyngeal cancers, large intestine cancers and laryngeal cancers (P>0.05). The TM levels incancer tissue extracts were significantly lower than that intheir adjacent non-cancer tissue extracts [(647.71317.51)mg/L vs (1455.63772.22)mg/L, P<0.01]. On the contrary, the plasma TM levels in these cancers were significantly higher than that in controls. Conclusion: The rise of plasma TMlevels in cancer patients was associated with metastasis and diffusion of cancers. The TM levels can be served as ansensitive index for judging progression and metastasis of
