Zoige wetland is one of the most important methane emission centers in China. The oxidation of methane in the wetland affects global warming, soil ecology and atmospheric chemistry. Despite their global significance, ...Zoige wetland is one of the most important methane emission centers in China. The oxidation of methane in the wetland affects global warming, soil ecology and atmospheric chemistry. Despite their global significance, microorganisms that consume methane in Zoige wetland remain poorly characterized. In this study, we investigated methanotrophs diversity in soil samples from both anaerobic site and aerobic site in Zoige wetland using pmoA gene as a molecular marker. The cloning library was constructed according to the pmoA sequences detected. Four clusters of methanotrophs were detected. The phylogenetic tree showed that all four clusters detected were affiliated to type I methanotrophs. Two novel clusters （cluster 1, cluster 2） were found to relate to none of the recognized genera of methanotrophs. These clusters have no cultured representatives and reveal an ecological adaptation of particular uncultured methanotrophs in Zoige wetland. Two clusters were belonging to Methylobacter and Methylococcus separately. Denaturing gradient gel electrophoresis gel bands pattern retrieved from these two samples revealed that the community compositions of anaerobic soil and aerobic soil were different from each other while anaerobic soil showed a higher metanotrophs diversity. Real-time PCR assays of the two samples demonstrated that aerobic soil sample in Zoige wetland was 1.5 times as much copy numbers as anaerobic soil. These data illustrated that methanotrophs are a group of microorganisms influence the methane consumption in Zoige wetland.展开更多
叶面积指数LAI(Leaf Area Index)是表征植被冠层结构的重要参数,然而由于云等大气因素的影响,MODISLAI时间序列产品在时间与空间尺度的连续性仍存在问题。随着先验知识在遥感反演中的地位不断得到加强,本文将多年LAI历史数据作为先验知...叶面积指数LAI(Leaf Area Index)是表征植被冠层结构的重要参数,然而由于云等大气因素的影响,MODISLAI时间序列产品在时间与空间尺度的连续性仍存在问题。随着先验知识在遥感反演中的地位不断得到加强,本文将多年LAI历史数据作为先验知识,用以建立LAI背景库,并提出了基于LAI背景库的Savitzky-Golay(SG)滤波算法来实现LAI时间序列数据的降噪工作。结果表明,与传统SG滤波相比,新算法能够更加客观有效地重建LAI时间序列。展开更多
基金supported by the Knowledge InnovationGrant of the Chinese Academy of Sciences (No. kzcx2-yw-418-03)
文摘Zoige wetland is one of the most important methane emission centers in China. The oxidation of methane in the wetland affects global warming, soil ecology and atmospheric chemistry. Despite their global significance, microorganisms that consume methane in Zoige wetland remain poorly characterized. In this study, we investigated methanotrophs diversity in soil samples from both anaerobic site and aerobic site in Zoige wetland using pmoA gene as a molecular marker. The cloning library was constructed according to the pmoA sequences detected. Four clusters of methanotrophs were detected. The phylogenetic tree showed that all four clusters detected were affiliated to type I methanotrophs. Two novel clusters （cluster 1, cluster 2） were found to relate to none of the recognized genera of methanotrophs. These clusters have no cultured representatives and reveal an ecological adaptation of particular uncultured methanotrophs in Zoige wetland. Two clusters were belonging to Methylobacter and Methylococcus separately. Denaturing gradient gel electrophoresis gel bands pattern retrieved from these two samples revealed that the community compositions of anaerobic soil and aerobic soil were different from each other while anaerobic soil showed a higher metanotrophs diversity. Real-time PCR assays of the two samples demonstrated that aerobic soil sample in Zoige wetland was 1.5 times as much copy numbers as anaerobic soil. These data illustrated that methanotrophs are a group of microorganisms influence the methane consumption in Zoige wetland.
文摘叶面积指数LAI(Leaf Area Index)是表征植被冠层结构的重要参数,然而由于云等大气因素的影响,MODISLAI时间序列产品在时间与空间尺度的连续性仍存在问题。随着先验知识在遥感反演中的地位不断得到加强,本文将多年LAI历史数据作为先验知识,用以建立LAI背景库,并提出了基于LAI背景库的Savitzky-Golay(SG)滤波算法来实现LAI时间序列数据的降噪工作。结果表明,与传统SG滤波相比,新算法能够更加客观有效地重建LAI时间序列。
