The study was undertaken to assess the genetic effect of quantitative trait loci （QTLs） conferring heat tolerance at flowering stage in rice. A population consisting of 279 F2 individuals from the cross between 996,...The study was undertaken to assess the genetic effect of quantitative trait loci （QTLs） conferring heat tolerance at flowering stage in rice. A population consisting of 279 F2 individuals from the cross between 996, a heat tolerant cultivar and 4628, a heat-sensitive cultivar, was analyzed for their segregation pattern of the difference of seed set rate under optimal temperature condition and high temperature condition. The difference of seed set rate under optimal temperature condition and high temperature condition showed normal distribution, indicating the polygenic control over the trait. To identify main effect of QTL for heat tolerance, the parents were surveyed with 200 primer pairs of simple sequence repeats （SSR）. The parental survey revealed 30% polymorphism between parents. In order to detect the main QTL association with heat tolerance, a strategy of combining the DNA pooling from selected segregants and genotyping was adopted. The association of putative markers identified based on DNA pooling from selected segregants was established by single marker analysis （SMA）. The results of SMA revealed that SSR markers, RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively, accounted for 17 and 3% of the total variation respectively. The heat tolerance during flowering stage in rice was controlled by multiple gene. The SSR markers, RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively, accounted for 17 and 3% of the total variation respectively. The two genetic loci, especially for RM3735 on chromosome 4, can be used in marker-assistant-selected method in heat tolerance breeding in rice.展开更多
Soybean mosaic virus （SMV） is one of the most broadly distributed diseases worldwide. It causes severe yield loss and seed quality deficiency in soybean （Glycine max （L.） Merr.）. SMV Strain SC14 isolated from Sh...Soybean mosaic virus （SMV） is one of the most broadly distributed diseases worldwide. It causes severe yield loss and seed quality deficiency in soybean （Glycine max （L.） Merr.）. SMV Strain SC14 isolated from Shanxi Province, China, was a newly identified virulent strain and can infect Kefeng No. 1, a source with wide spectrum resistance. In the present study, soybean accessions, PI96983, Qihuang No. 1 and Qihuang No. 22 were identified to be resistant （R） and Nannong 1138-2, Pixianchadou susceptible （S） to SC14. Segregation analysis of PI96983 x Nannong 1138-2 indicated that a single dominant gene （designated as Rsc14） controlled the resistance to SC14 at both V2 and R1 developmental stages. The same results were obtained for the crosses of Qihuang No. 1 × Nannong 1138-2 and Qihuang No. 22 x Nannong 1138-2 as in PI96983 x×Nannong 1138-2 at V2 stage, but at R1 stage, the F1 performed as necrosis （a susceptible symptom other than mosaic）, F2 segregated in a ratio of 1R：2N：IS, and the progenies of necrotic （N） F2 individuals segregated also in R, N and S. It indicated that a single gene （designated as Rsc140, to be different from that of PI96983） controlled the resistance to SC14, its dominance was the same as in PI96983 × Nannong 1138-2 （without symptoms） at V2 stage and not the same at R1 stage. The tightly linked co-dominant simple sequence repeat （SSR） marker Satt334 indicated that all the heterozygous bands were completely corresponding to the necrotic F2 individuals, or all the necrotic F2 individuals were heterozygotes. It was inferred that necrosis might be due to the interaction among SMV strains, resistance genes, genetic background of the resistance genes, and plant development stage. Furthermore, the bulked segregant analysis （BSA） of SSR markers was conducted to map the resistance genes. In F2 of PI96983 × Nannong 1138-2, five SSR markers, Sat_297, Sat_234, Sat_154, Sct_033 and Sat_120, were found closely linked to Rsc14, with genetic distances of展开更多
采用微卫星分子标记(SSR)技术分析大菱鲆(Scophthalmus maximus L.)耐高温相关特性,为耐高温大菱鲆的分子辅助育种提供合适的分子标记。将大菱鲆经过高温实验处理,区分为耐高温组与高温敏感组,用于实验分析。采用Salah.M法抽提大菱鲆肌...采用微卫星分子标记(SSR)技术分析大菱鲆(Scophthalmus maximus L.)耐高温相关特性,为耐高温大菱鲆的分子辅助育种提供合适的分子标记。将大菱鲆经过高温实验处理,区分为耐高温组与高温敏感组,用于实验分析。采用Salah.M法抽提大菱鲆肌肉组织的DNA,根据已知的30个大菱鲆微卫星位点的侧翼保守序列设计引物,进行微卫星引物PCR(SSR-PCR)扩增。对PCR扩增出的差异条带进行个体统计,最后进行微卫星位点与耐高温性状的相关性分析。结果表明,有1个微卫星位点与大菱鲆耐高温性状存在一定的负相关性;有3个微卫星位点与大菱鲆耐高温性状存在正相关性,其中位点Sma-USC27 286 bp的等位基因片段与耐高温性状的正相关性极显著,相关系数达到0.383(P<0.01),其余2个位点为一般显著性相关。微卫星位点Sma-USC27所扩增出的差异等位基因片段可作为分子标记指导耐高温大菱鲆的辅助育种。展开更多
A qualitative trait is usually controlled by a single gene, but it may be sometimes controlled by two or even more genes. This phenomenon is called gene interaction. Rapidly searching for linked mo- lecular markers vi...A qualitative trait is usually controlled by a single gene, but it may be sometimes controlled by two or even more genes. This phenomenon is called gene interaction. Rapidly searching for linked mo- lecular markers via bulked segregant analysis (BSA) and then constructing regional linkage map with Mapmaker/Exp has become a common approach to mapping single major genes. However, methods and computer programs developed for mapping single major genes cannot be simply applied to interactive genes because the genetic patterns of gene interac- tions are quite different from that of single-gene in- heritance. Up to now, experimental methods for quickly screening molecular markers linked to inter- active genes and statistical methods and corre- sponding computer softwares for simultaneously analyzing the linkage relationships of multiple mo- lecular markers to an interactive gene have not been available. To solve this problem, in this paper, we propose a strategy for mapping interactive genes using BSA and Mapmaker/Exp. We demonstrate that all interactive genes can be mapped by the 'BSA + Mapmaker/Exp' strategy using F2 generation (in a few cases, F3 generation is also needed). As BSA and Mapmaker/Exp have been broadly used in gene mapping studies and are well known by many re- searchers, the strategies proposed in this paper will be useful for practical researches.展开更多
Head smut of maize （Zea mays L.）, which was caused by Sporisorium reiliana, occurred in most of the maize growing areas of the world. The purpose of this study was to develop SCAR markers for map-based cloning of re...Head smut of maize （Zea mays L.）, which was caused by Sporisorium reiliana, occurred in most of the maize growing areas of the world. The purpose of this study was to develop SCAR markers for map-based cloning of resistance genes and MAS. Two sets of BC3 progenies, one （BC3Q） derived from the cross Qi319 （resistance）×Huangzao 4 （susceptible）, the other （BC3M） from Mol7 （resistance）× Huangzao 4 （susceptible）, were generated. Huangzao 4 was the recurrent parent in both progenies. A combination of BSA （bulked segregant analysis） with AFLP （amplified fragment length polymorphism） method was applied to map the genes involving the resistance to S. reiliana, and corresponding resistant and susceptible bulks and their parental lines were used for screening polymorphic AFLP primer pairs. One fragment of PI3M61-152 was converted into SCAR （sequence charactered amplified fragment） marker S130. The marker was mapped at chromosome bin 2.09, the interval of a major QTL region previously reported to contribute to S. reiliana resistance. Furthermore, S130 was highly and facilitate map-based cloni associated with resistance to S. reiliana, and could be useful for marker-assisted selection ng of resistance genes.展开更多
基金supported by the National Natural Science Foundation of China (30500315)Transformation of Agricultural Scientific and Technological Achievements Program from the Ministry of Science and Technology of China (05EFN214300193)Educational Foundation of Hunan Province,China (07C360)
文摘The study was undertaken to assess the genetic effect of quantitative trait loci （QTLs） conferring heat tolerance at flowering stage in rice. A population consisting of 279 F2 individuals from the cross between 996, a heat tolerant cultivar and 4628, a heat-sensitive cultivar, was analyzed for their segregation pattern of the difference of seed set rate under optimal temperature condition and high temperature condition. The difference of seed set rate under optimal temperature condition and high temperature condition showed normal distribution, indicating the polygenic control over the trait. To identify main effect of QTL for heat tolerance, the parents were surveyed with 200 primer pairs of simple sequence repeats （SSR）. The parental survey revealed 30% polymorphism between parents. In order to detect the main QTL association with heat tolerance, a strategy of combining the DNA pooling from selected segregants and genotyping was adopted. The association of putative markers identified based on DNA pooling from selected segregants was established by single marker analysis （SMA）. The results of SMA revealed that SSR markers, RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively, accounted for 17 and 3% of the total variation respectively. The heat tolerance during flowering stage in rice was controlled by multiple gene. The SSR markers, RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively, accounted for 17 and 3% of the total variation respectively. The two genetic loci, especially for RM3735 on chromosome 4, can be used in marker-assistant-selected method in heat tolerance breeding in rice.
基金Supported by the State Key Basic Research and Development Plan of China （2004CB117203-2 and 2002CB111304）, the National Natural Science Foundation of China （30571176 and 30490250）, the Natural Science Foundation of Jiangsu Province （BK2004100） and the Program for Changjiang Scholars and Innovative Research Team in University （PCSIRT）.
文摘Soybean mosaic virus （SMV） is one of the most broadly distributed diseases worldwide. It causes severe yield loss and seed quality deficiency in soybean （Glycine max （L.） Merr.）. SMV Strain SC14 isolated from Shanxi Province, China, was a newly identified virulent strain and can infect Kefeng No. 1, a source with wide spectrum resistance. In the present study, soybean accessions, PI96983, Qihuang No. 1 and Qihuang No. 22 were identified to be resistant （R） and Nannong 1138-2, Pixianchadou susceptible （S） to SC14. Segregation analysis of PI96983 x Nannong 1138-2 indicated that a single dominant gene （designated as Rsc14） controlled the resistance to SC14 at both V2 and R1 developmental stages. The same results were obtained for the crosses of Qihuang No. 1 × Nannong 1138-2 and Qihuang No. 22 x Nannong 1138-2 as in PI96983 x×Nannong 1138-2 at V2 stage, but at R1 stage, the F1 performed as necrosis （a susceptible symptom other than mosaic）, F2 segregated in a ratio of 1R：2N：IS, and the progenies of necrotic （N） F2 individuals segregated also in R, N and S. It indicated that a single gene （designated as Rsc140, to be different from that of PI96983） controlled the resistance to SC14, its dominance was the same as in PI96983 × Nannong 1138-2 （without symptoms） at V2 stage and not the same at R1 stage. The tightly linked co-dominant simple sequence repeat （SSR） marker Satt334 indicated that all the heterozygous bands were completely corresponding to the necrotic F2 individuals, or all the necrotic F2 individuals were heterozygotes. It was inferred that necrosis might be due to the interaction among SMV strains, resistance genes, genetic background of the resistance genes, and plant development stage. Furthermore, the bulked segregant analysis （BSA） of SSR markers was conducted to map the resistance genes. In F2 of PI96983 × Nannong 1138-2, five SSR markers, Sat_297, Sat_234, Sat_154, Sct_033 and Sat_120, were found closely linked to Rsc14, with genetic distances of
文摘采用微卫星分子标记(SSR)技术分析大菱鲆(Scophthalmus maximus L.)耐高温相关特性,为耐高温大菱鲆的分子辅助育种提供合适的分子标记。将大菱鲆经过高温实验处理,区分为耐高温组与高温敏感组,用于实验分析。采用Salah.M法抽提大菱鲆肌肉组织的DNA,根据已知的30个大菱鲆微卫星位点的侧翼保守序列设计引物,进行微卫星引物PCR(SSR-PCR)扩增。对PCR扩增出的差异条带进行个体统计,最后进行微卫星位点与耐高温性状的相关性分析。结果表明,有1个微卫星位点与大菱鲆耐高温性状存在一定的负相关性;有3个微卫星位点与大菱鲆耐高温性状存在正相关性,其中位点Sma-USC27 286 bp的等位基因片段与耐高温性状的正相关性极显著,相关系数达到0.383(P<0.01),其余2个位点为一般显著性相关。微卫星位点Sma-USC27所扩增出的差异等位基因片段可作为分子标记指导耐高温大菱鲆的辅助育种。
文摘A qualitative trait is usually controlled by a single gene, but it may be sometimes controlled by two or even more genes. This phenomenon is called gene interaction. Rapidly searching for linked mo- lecular markers via bulked segregant analysis (BSA) and then constructing regional linkage map with Mapmaker/Exp has become a common approach to mapping single major genes. However, methods and computer programs developed for mapping single major genes cannot be simply applied to interactive genes because the genetic patterns of gene interac- tions are quite different from that of single-gene in- heritance. Up to now, experimental methods for quickly screening molecular markers linked to inter- active genes and statistical methods and corre- sponding computer softwares for simultaneously analyzing the linkage relationships of multiple mo- lecular markers to an interactive gene have not been available. To solve this problem, in this paper, we propose a strategy for mapping interactive genes using BSA and Mapmaker/Exp. We demonstrate that all interactive genes can be mapped by the 'BSA + Mapmaker/Exp' strategy using F2 generation (in a few cases, F3 generation is also needed). As BSA and Mapmaker/Exp have been broadly used in gene mapping studies and are well known by many re- searchers, the strategies proposed in this paper will be useful for practical researches.
基金funded by the National Hi-Tech R&D Program,China(863Program,2006AA100103,2007AA10Z172)the International Cooperation Project for Science and Technology(2007DFA31010)
文摘Head smut of maize （Zea mays L.）, which was caused by Sporisorium reiliana, occurred in most of the maize growing areas of the world. The purpose of this study was to develop SCAR markers for map-based cloning of resistance genes and MAS. Two sets of BC3 progenies, one （BC3Q） derived from the cross Qi319 （resistance）×Huangzao 4 （susceptible）, the other （BC3M） from Mol7 （resistance）× Huangzao 4 （susceptible）, were generated. Huangzao 4 was the recurrent parent in both progenies. A combination of BSA （bulked segregant analysis） with AFLP （amplified fragment length polymorphism） method was applied to map the genes involving the resistance to S. reiliana, and corresponding resistant and susceptible bulks and their parental lines were used for screening polymorphic AFLP primer pairs. One fragment of PI3M61-152 was converted into SCAR （sequence charactered amplified fragment） marker S130. The marker was mapped at chromosome bin 2.09, the interval of a major QTL region previously reported to contribute to S. reiliana resistance. Furthermore, S130 was highly and facilitate map-based cloni associated with resistance to S. reiliana, and could be useful for marker-assisted selection ng of resistance genes.
