The ultrastructure of the mature egg and fertilization in the fern Ceratopteris thafictroides （L.） Brongn. were observed by transmission electron microscopy. The results revealed that the mature egg possesses an obv...The ultrastructure of the mature egg and fertilization in the fern Ceratopteris thafictroides （L.） Brongn. were observed by transmission electron microscopy. The results revealed that the mature egg possesses an obvious egg membrane at the periphery of the egg. Furthermore, a fertilization pore was identified in the upper egg membrane of the mature egg. The structure of the pore is described for the first time. The fertilization experiment indicated that spermatozoids crowd into the cavity above the egg through the neck canal of the archegonium; however, only one of these can penetrate into the egg through the fertilization pore. Immediately on penetration of the spermatozoid, the egg begins to shrink. The volume of the fertilized egg decreases to almost one-half that of the unfertilized egg. As a result, the protoplasm of the fertilized egg becomes dense and opaque, which may lead to a situation where the organelles of both the egg and the fertilizing spermatozoid become indistinguishable. Simultaneously, abundant vesicles containing concentric membranes or opaque materials appear near the fertilization pore in the cytoplasm of the fertilized egg. These vesicles are considered to act as a barrier that prevents polyspermy. The present study provides a new insight into the ultrastructure of the mature egg and the cytological mechanism of fertilization in ferns.展开更多
[ Objective] This study was to analyze the two hydrochemical factors affecting the distribution of Ceratopteris thalictroides, further to provide basis for its habitat protection and population restoration. [ Method ]...[ Objective] This study was to analyze the two hydrochemical factors affecting the distribution of Ceratopteris thalictroides, further to provide basis for its habitat protection and population restoration. [ Method ] Twenty two hydrochemical parameters of 22 sampling sites in China＇s tropical and subtropical zones were respectively averaged for calculating their variation coefficients, of which the two showing significant differences were used for multiple comparisons and correlation analyses via least significant difference and correlation coefficient. E Result ] The correlation coefficients of all the 22 tested hydrochemical parameters varied hugely, but only pH value and conductivity showed significantly differences in two water body （type A and type B）. Analysis of correlation presented that pH value were positively correlated with conductivity; further the multiple comparisons showed that the significant difference of pH value was higher than that of conductivity. [Conclusion] With regard to the two hydrochemical parameters showing significant differences, pH value influences the distribution of C. thalictroides more.展开更多
基金Supported by the National Natural Science Foundation of China (30670128).
文摘The ultrastructure of the mature egg and fertilization in the fern Ceratopteris thafictroides （L.） Brongn. were observed by transmission electron microscopy. The results revealed that the mature egg possesses an obvious egg membrane at the periphery of the egg. Furthermore, a fertilization pore was identified in the upper egg membrane of the mature egg. The structure of the pore is described for the first time. The fertilization experiment indicated that spermatozoids crowd into the cavity above the egg through the neck canal of the archegonium; however, only one of these can penetrate into the egg through the fertilization pore. Immediately on penetration of the spermatozoid, the egg begins to shrink. The volume of the fertilized egg decreases to almost one-half that of the unfertilized egg. As a result, the protoplasm of the fertilized egg becomes dense and opaque, which may lead to a situation where the organelles of both the egg and the fertilizing spermatozoid become indistinguishable. Simultaneously, abundant vesicles containing concentric membranes or opaque materials appear near the fertilization pore in the cytoplasm of the fertilized egg. These vesicles are considered to act as a barrier that prevents polyspermy. The present study provides a new insight into the ultrastructure of the mature egg and the cytological mechanism of fertilization in ferns.
基金Supported by National Natural Science Foundation of China(30370098)~~
文摘[ Objective] This study was to analyze the two hydrochemical factors affecting the distribution of Ceratopteris thalictroides, further to provide basis for its habitat protection and population restoration. [ Method ] Twenty two hydrochemical parameters of 22 sampling sites in China＇s tropical and subtropical zones were respectively averaged for calculating their variation coefficients, of which the two showing significant differences were used for multiple comparisons and correlation analyses via least significant difference and correlation coefficient. E Result ] The correlation coefficients of all the 22 tested hydrochemical parameters varied hugely, but only pH value and conductivity showed significantly differences in two water body （type A and type B）. Analysis of correlation presented that pH value were positively correlated with conductivity; further the multiple comparisons showed that the significant difference of pH value was higher than that of conductivity. [Conclusion] With regard to the two hydrochemical parameters showing significant differences, pH value influences the distribution of C. thalictroides more.
